126 research outputs found
Characterization of anogenital distance and its relationship to fertility in lactating Holstein cows
peer-reviewedAnogenital distance (AGD) serves as a marker for prenatal androgenization, reproductive development, and fertility in humans and rodents. The primary objectives of this observational study in lactating dairy cows were to (1) characterize the distribution and variability of AGD, (2) determine the relationship among AGD and potential postnatal AGD determinants of age and height, and (3) evaluate the associations between AGD and pregnancy to first artificial insemination (P/AI) and cumulative pregnancy by 250 d in milk (DIM) within parity groups (first, second, and third+ parities). The secondary objective was to evaluate the association between AGD and testosterone concentrations. The AGD (mm), age (yr), and height at hip (cm) at the time of AGD determination, and aforesaid reproductive outcomes were determined in 921 Holstein cows (first, second, and third+ parity; n = 360, 256, and 305, respectively). Plasma concentrations of testosterone were determined in a subset of 93 cows. Overall, AGD had a normal distribution and high variability [mean (±standard deviation); 131.0 ± 12.2 mm], was weakly associated with cow age and height (coefficient of determination = 0.09 and 0.04, respectively), and had an inverse relationship with P/AI in first- and second-parity cows, but not in third+ parity cows. For every 1 mm increase in AGD, the odds of P/AI decreased by 3.4 and 2.4% for first- and second-parity cows, respectively. The optimal AGD threshold to predict probability of P/AI was 127.1 mm for both first- (sensitivity: 66.4; specificity: 56.6%) and second-parity cows (sensitivity: 46.0; specificity: 70.4%). Accordingly, first- and second-parity cows were categorized into either short or long AGD (≤ or >127.1 mm), and associations with reproductive outcomes were evaluated. First-parity cows with long AGD had lower P/AI (30.9 vs. 53.6%) and decreased likelihood (hazard ratio: 0.68) of pregnancy by 250 DIM than those with short AGD. Similarly, second-parity cows with long AGD had reduced P/AI (28.3 vs. 44.4%) and a tendency for decreased likelihood (hazard ratio: 0.76) of pregnancy by 250 DIM than in cows with short AGD. The association between AGD and testosterone was weak and nonsignificant. In summary, AGD in Holstein cows was normally distributed, highly variable, and weakly associated with age and height. Besides, AGD had an inverse relationship with P/AI and cumulative pregnancy by 250 DIM in first- and second-parity cows; however, such a relationship was not evident in older (third+ parity) cows.This project was financially supported in part by Growing Forward 2, a federal-provincial-territorial initiative (Research Opportunities and Innovation–Internal Initiatives Project # DA7642064). M. Gobikrushanth is a recipient of the Teagasc-University of Alberta Walsh Fellowship sponsored by Alberta Innovates Biosolutions
The relationship between serum insulin-like growth factor-1 (IGF-1) concentration and reproductive performance, and genome-wide associations for serum IGF-1 in Holstein cows
peer-reviewedThe objectives of this study were to determine (1) factors associated with serum concentration of insulin-like growth factor-1 (IGF-1); (2) the relationship between serum IGF-1 concentration during the first week postpartum and ovarian cyclicity status by 35 d postpartum (DPP); (3) an optimum serum IGF-1 concentration threshold predictive of pregnancy to first artificial insemination (P/AI), including its diagnostic values; (4) the associations among categories of serum IGF-1 concentration and reproductive outcomes (P/AI and pregnancy risk up to 150 and 250 DPP); and (5) single nucleotide polymorphisms (SNP) associated with phenotypic variation in serum IGF-1 concentration in dairy cows. Serum IGF-1 concentration was determined at 7 (±2.4; ±standard error of the mean) DPP in 647 lactating Holstein cows (213 primiparous, 434 multiparous) from 7 herds in Alberta, Canada. The overall mean, median, minimum, and maximum serum IGF-1 concentrations during the first week postpartum were 37.8 (±1.23), 31.0, 20.0, and 225.0 ng/mL, respectively. Herd, age, parity, precalving body condition score, and season of blood sampling were all identified as factors associated with serum IGF-1 concentrations. Although serum IGF-1 concentration during the first week postpartum had no association with ovarian cyclicity status by 35 DPP in primiparous cows, it was greater in cyclic than in acyclic multiparous cows (32.2 vs. 27.4 ng/mL, respectively). The optimum serum IGF-1 thresholds predictive of P/AI were 85.0 ng/mL (sensitivity = 31.9%; specificity = 89.1%) and 31.0 ng/mL (sensitivity = 45.5%; specificity = 66.9%) for primiparous and multiparous cows, respectively. When cows were grouped into either high or low IGF-1 categories (greater or less than or equal to 85.0 ng/mL for primiparous cows and greater or less than or equal to 31.0 ng/mL for multiparous cows, respectively), primiparous cows with high IGF-1 had 4.43 times greater odds of P/AI and a tendency for higher pregnancy risk up to 150 DPP than those with low IGF-1, but not up to 250 DPP. Likewise, multiparous cows with high IGF-1 had 1.61 times greater odds of P/AI than those with low IGF-1. Pregnancy risk up to 150 and 250 DPP, however, did not differ between IGF-1 categories in multiparous cows. Moreover, 37 SNP across 10 Bos taurus autosomes were associated with variation in serum IGF-1 concentration, and 4 previously identified candidate genes related to fertility that were in linkage disequilibrium with some of these SNP were also identified
Active galactic nuclei, gravitational redshifts, and cosmological tensions
Gravitational redshift is a classical effect of Einstein's General
Relativity, already measured in stars, quasars and clusters of galaxies. We
here identify the signature of gravitational redshift in the emission lines of
active galaxies due to supermassive black holes and discuss their impact on
cosmological inference from type Ia supernovae. Firstly, from the full width at
half maximum of lines of 75 Seyfert type I galaxies of the AGN
Black Hole Mass Database, we derive a gravitational redshift . Expanding this analysis to 86755 quasars from DR14 of
SDSS we have a mean value . Then, by comparing
the redshifts of 34 lines measured at the central and outer regions of LINER
galaxies in the SAMI survey we obtain .
These numbers are compatible with central black holes of solar
masses and broad line regions of ~pc. For non-AGN galaxies the
gravitational redshift is compatible with zero and, as they constitute most of
SNe Ia host galaxies, the impact on the cosmological parameters is negligible.Comment: 5 pages, 5 figure
Scalable background-limited polarization-sensitive detectors for mm-wave applications
We report on the status and development of polarization-sensitive detectors
for millimeter-wave applications. The detectors are fabricated on
single-crystal silicon, which functions as a low-loss dielectric substrate for
the microwave circuitry as well as the supporting membrane for the
Transition-Edge Sensor (TES) bolometers. The orthomode transducer (OMT) is
realized as a symmetric structure and on-chip filters are employed to define
the detection bandwidth. A hybridized integrated enclosure reduces the
high-frequency THz mode set that can couple to the TES bolometers. An
implementation of the detector architecture at Q-band achieves 90% efficiency
in each polarization. The design is scalable in both frequency coverage, 30-300
GHz, and in number of detectors with uniform characteristics. Hence, the
detectors are desirable for ground-based or space-borne instruments that
require large arrays of efficient background-limited cryogenic detectors.Comment: 7 pages, 3 figures, Presented at SPIE Astronomical Telescopes and
Instrumentation 2014: Millimeter, Submillimeter, and Far-Infrared Detectors
and Instrumentation for Astronomy VII. To be published in Proceedings of SPIE
Volume 915
Silicon-Based Antenna-Coupled Polarization-Sensitive Millimeter-Wave Bolometer Arrays for Cosmic Microwave Background Instruments
We describe feedhorn-coupled polarization-sensitive detector arrays that
utilize monocrystalline silicon as the dielectric substrate material.
Monocrystalline silicon has a low-loss tangent and repeatable dielectric
constant, characteristics that are critical for realizing efficient and uniform
superconducting microwave circuits. An additional advantage of this material is
its low specific heat. In a detector pixel, two Transition-Edge Sensor (TES)
bolometers are antenna-coupled to in-band radiation via a symmetric planar
orthomode transducer (OMT). Each orthogonal linear polarization is coupled to a
separate superconducting microstrip transmission line circuit. On-chip
filtering is employed to both reject out-of-band radiation from the upper band
edge to the gap frequency of the niobium superconductor, and to flexibly define
the bandwidth for each TES to meet the requirements of the application. The
microwave circuit is compatible with multi-chroic operation. Metalized silicon
platelets are used to define the backshort for the waveguide probes. This
micro-machined structure is also used to mitigate the coupling of out-of-band
radiation to the microwave circuit. At 40 GHz, the detectors have a measured
efficiency of 90%. In this paper, we describe the development of the 90 GHz
detector arrays that will be demonstrated using the Cosmology Large Angular
Scale Surveyor (CLASS) ground-based telescope
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