6 research outputs found

    Determination of Coenzyme A and Acetyl-Coenzyme A in Biological samples Using HPLC with UV Detection

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    Coenzyme A (CoA) and acetyl-coenzyme A (acetyl-CoA) play essential roles in cell energy metabolism. Dysregulation of the biosynthesis and functioning of both compounds may contribute to various pathological conditions. We describe here a simple and sensitive HPLC-UV based method for simultaneous determination of CoA and acetyl-CoA in a variety of biological samples, including cells in culture, mouse cortex, and rat plasma, liver, kidney, and brain tissues. The limits of detection for CoA and acetyl-CoA are \u3e10-fold lower than those obtained by previously described HPLC procedures, with coefficients of variatio

    Mitophagy plays a central role in mitochondrial ageing

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    The potential of endurance exercise-derived exosomes to treat metabolic diseases

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    Feeding in cartilaginous fishes: An interdisciplinary synthesis

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