Crystal structure of nucleotide-free dynamin

Dynamin is a mechanochemical GTPase that oligomerizes around the neck of clathrin-coated pits and catalyses vesicle scission in a GTP-hydrolysis-dependent manner. The molecular details of oligomerization and the mechanism of the mechanochemical coupling are currently unknown. Here we present the crystal structure of human dynamin 1 in the nucleotide-free state with a four-domain architecture comprising the GTPase domain, the bundle signalling element, the stalk and the pleckstrin homology domain. Dynamin 1 oligomerized in the crystals via the stalks, which assemble in a criss-cross fashion. The stalks further interact via conserved surfaces with the pleckstrin homology domain and the bundle signalling element of the neighbouring dynamin molecule. This intricate domain interaction rationalizes a number of disease-related mutations in dynamin 2 and suggests a structural model for the mechanochemical coupling that reconciles previous models of dynamin function

Can mHealth Technology Help Mitigate the Effects of the COVID-19 Pandemic?

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Microprocessor Coding For Biometric Device

The objective of this research project was to create a wearable device that monitors bodily functions for the user to view on their smartphone. Sensor data is processed using the Arduino Nano 33 BLE microcontroller. The sensors used in this project include proximity, temperature, humidity, heart rate, pressure, and skin impedance. This project takes advantage of the Arduinos Bluetooth low energy (BLE) capabilities so that all the data can be transmitted to a smartphone. This presentation examines the challenges of programming these devices, such as sensor timing, challenges related to heart rate processing, and Bluetooth transmission. Even though the device worked correctly, using two Arduinos to process the data doubles the power consumption. The signal processing may be improved upon by using a single device which contains two processing cores

Is objective testing for menorrhagia in general practice practical?: results from a qualitative study

Objectives: to explore the interpretative character of medical knowledge and the way that clinicians respond to the patient's assertion that her menstrual blood loss is excessive. In particular, we are interested in the boundary between ‘normal’ and ‘abnormal’, and whether or not general practitioners would consider conducting objective tests for menorrhagia in their surgeries. We also wanted to explore the extent to which clinicians pay attention to women's subjective accounts of ‘heavy’ menstrual blood loss when making a diagnosis. The consequences of making a distinction between ‘normal’ and ‘abnormal’ blood loss may be considerable. A diagnosis of menorrhagia may crucially affect quality of life, morbidity and mortality.Method: qualitative study using 73 semi-structured interviews with general practitioners in Northwest England.Results: two thirds of the respondents indicated that they seriously attempt an assessment of menstrual blood loss, while one third of the respondents appeared to pay more attention to the women's subjective assessment of unacceptable ‘heavy’ bleeding. Some general practitioners had a very negative attitude to menstrual blood. Very few would consider conducting objective tests for menstrual blood loss if such tests involved the collection of soiled pads and tampons. However, about half of the respondents thought that a pictorial chart might be useful when trying to estimate menstrual blood loss.Conclusion: since general practitioners are not in agreement about the manner in which women's complaints of heavy bleeding should be assessed, evidence-based clinical guidelines that deal with both ‘subjective’ and ‘objective’ menorrhagia are timel