Increased expression of circulating miRNA-93 in women with polycystic ovary syndrome may represent a novel, non-invasive biomarker for diagnosis

MicroRNAs (miRNA) are a novel class of small noncoding single-stranded RNA molecules that regulate gene expression. There is increasing evidence of their importance in polycystic ovary syndrome (PCOS). The objective was to determine if miRNA-93 and miRNA-223 are differentially expressed in the circulation of women with PCOS compared to age matched women. A case–control study comparing women with PCOS (n = 25) to age and weight matched controls (n = 24) without PCOS was performed. MiRNA-93 and miRNA-223 were determined by total RNA reverse transcription. Both miRNA-93 and miRNA-223 were significantly increased relative to the control group (p < 0.01, p = 0.029 respectively). In both groups there was no correlation of either miRNA-93 or miRNA-223 with insulin, HOMA-IR, HOMA-β or testosterone levels. The area under the receiver operator characteristic curve for miR-223 and miR-93 was 0.66 and 0.72 respectively, suggesting miR-93 is a more efficient biomarker than miR-223 for diagnosis of PCOS. The combination of the two miRNAs together, tested using multiple logistic regression analysis, did not improve the diagnostic potential. In conclusion, circulating miRNA-93 and miRNA-223 were higher in women with PCOS compared to age and weight matched controls independent of insulin resistance and testosterone levels, and miR-93 may represent a novel diagnostic biomarker for PCOS

Newspaper Readership Among College Students in the Information Age: The Influence of Telecommunication Technology

This article focuses on the influence of telecommunication technology on newspaper readership among college students in the U.S. during the 1990s. The findings presented suggest an explanatory role for such factors as age in readership. Income and marital status are also important correlates of readership, perhaps because they gauge one\u27s stake or integration in the local community. The resulting loss of afternoon leisure was a leading cause in the decline of afternoon papers, which have been substituted with nightly TV news reporting. Given the role that demographic and media use variables play in newspaper readership, it will be important to consider the influence of other lifestyle characteristics

Expression and function of transient receptor potential channels in the female bovine reproductive tract

© 2016 Elsevier Inc. The epithelium lining the oviduct is critical for early reproductive events, many of which are mediated via intracellular calcium ions. Despite this, little is known about the regulation of calcium homeostasis in the oviductal epithelium. Epithelial transient receptor potential channels (TRPCs) modulate calcium flux in other tissues, and their expression and functional regulation have therefore been examined using the bovine oviduct as a model for the human. The effects of FSH, LH, 17β-estradiol, and progesterone on TRPCs expression and intracellular calcium flux were determined. Transient receptor potential channels 1, 2, 3, 4, and 6 were expressed in the bovine reproductive tract, and their gene expression varied throughout the estrous cycle. In more detailed studies undertaken on TRPC1 and 6, we show that protein expression varied through the estrus cycle; specifically, 17β-estradiol, FSH, and LH individually and in combination upregulated TRPC1 and 6 expression in cultured bovine oviduct epithelial cells although progesterone antagonized these effects. Functional studies showed changes in calcium mobilization in bovine oviduct epithelial cells were dependent on TRPCs. In conclusion, TRPC1, 2, 3, 4, and 6 are present in the epithelium lining the bovine oviduct, and TRPC1 and 6 vary through the estrous cycle suggesting an important role in early reproductive function

The trade-off between taxi time and fuel consumption in airport ground movement

Environmental impact is a very important agenda item in many sectors nowadays, which the air transportation sector is also trying to reduce as much as possible. One area which has remained relatively unexplored in this context is the ground movement problem for aircraft on the airport’s surface. Aircraft have to be routed from a gate to a runway and vice versa and it is still unknown whether fuel burn and environmental impact reductions will best result from purely minimising the taxi times or whether it is also important to avoid multiple acceleration phases. This paper presents a newly developed multi-objective approach for analysing the trade-off between taxi time and fuel consumption during taxiing. The approach consists of a combination of a graph-based routing algorithm and a population adaptive immune algorithm to discover different speed profiles of aircraft. Analysis with data from a European hub airport has highlighted the impressive performance of the new approach. Furthermore, it is shown that the trade-off between taxi time and fuel consumption is very sensitive to the fuel-related objective function which is used

'A light in a very dark place' : The role of a voluntary organisation providing support for those affected by encephalitis

Voluntary organisations are seen as contributing to the ‘democratisation’ of health and social care. Little, however, is written about their role and this paper, by focusing on the work of The Encephalitis Society, provides insights into the challenges facing voluntary organisations as they manage twin roles as service providers and advocates, of people with neurological disorders. Two studies are presented: a review conducted by the Society, focusing on patient’s experiences of neurological services; and an external evaluation of the Society’s current provision. The first, based on a postal survey of its members affected by encephalitis (n = 339), illustrates the Society’s advocacy role. The survey provided support for the Association of British Neurologists’ recommendation for nationally agreed standards of care. The second study, a postal survey of recent contacts (n = 76) and in-depth telephone interviews (n = 22), illustrates the Society’s value role as a service provider and supports its role in helping rehabilitate affected individuals and their families. These studies provided the Society with information for policy and service development. Importantly, providing the basis of informed action and partnership with stakeholders and informing the organisation’s sense of purpose, in the changing context of welfare provision in the UK

School gate to dinner plate : sedentary and physically active behaviours in adolescents after school

Against a backdrop of rising overweight and obesity, there is a need to further our understanding of physical activity and sedentary behaviour patterns in young people to aid the development of behaviour change strategies that may bring about an increase in energy expenditure. Sedentary and active behaviours exhibit temporal patterning, thus it may be beneficial to examine behaviour and associations between behaviours during specific periods of the day This thesis presents five studies that examine sedentary and physically active behaviours in adolescents during the three hours immediately after school. Chapter 2, using data from a large study of adolescents in the UK, describes physical activity and sedentary behaviour patterns between 15.30h - 18.30h, and examines the contribution of this period relative to broader leisure-time behaviour patterns. In chapter 3.1, the' associations between a broad range of sedentary behaviours and objectively assessed physical activity are examined during the after school hours, providing unique insight into the interactions between behaviours at this time. Chapter 3.2 explores the social and environmental context of selected sedentary and active behaviours after school, enabling a more complete understanding of where and with whom young people spend their time during these hours. Chapter 4 is a systematic literature review of interventions to increase physical activity in young people conducted in the hours immediately after school. Together, the four studies described above established a rationale and informed the content of a pilot family-based intervention to reduce screen-time and increase physical activity after school, presented in Chapter 5. Approximately 40% of adolescents' leisure-time physical activity occurs in the three hours immediately after school, suggesting that this is a critical period in which young people obtain a significant proportion of their daily leisure-time activity. However, sedentary behaviours, particularly screen-based media, account for the majority of time-use during these hours, and may displace participation in physical activity. The development of time-targeted intervention strategies, with a focus upon the after school period, holds considerable promise for the promotion of physical activity in young people

Effects of human recombinant growth hormone on exercise capacity, cardiac structure, and cardiac function in patients with adult-onset growth hormone deficiency

Objective Epidemiological studies suggest that adult-onset growth hormone deficiency (AGHD) might increase the risk of death from cardiovascular causes. Methods This was a 6-month double-blind, placebo-controlled, randomised, cross-over trial followed by a 6-month open-label phase. Seventeen patients with AGHD received either recombinant human growth hormone (rGH) (0.4 mg injection daily) or placebo for 12 weeks, underwent washout for 2 weeks, and were then crossed over to the alternative treatment for a further 12 weeks. Cardiac magnetic resonance imaging, echocardiography, and cardiopulmonary exercise testing were performed at baseline, 12 weeks, 26 weeks, and the end of the open phase (12 months). The results were compared with those of 16 age- and sex-matched control subjects. Results At baseline, patients with AGHD had a significantly higher systolic blood pressure, ejection fraction, and left ventricular mass than the control group, even when corrected for body surface area. Treatment with rGH normalised the insulin-like growth factor 1 concentration without an effect on exercise capacity, cardiac structure, or cardiac function. Conclusion Administration of rGH therapy for 6 to 9 months failed to normalise the functional and structural cardiac differences observed in patients with AGHD when compared with a control group

Measurement and modelling of the turbulent boundary layer near the attachment line of a swept wing

This work is motivated by the need for low-order aerodynamic models to predict accurately the effect on profile drag of controlling attachment line transition. Head's entrainment method (1) , a rapid integral boundary layer technique used for design studies on swept wings, suffers from the governing swept-tapered turbulent integral boundary layer equations being ill-posed in the vicinity of the attachment line. This singularity has been treated using crude extrapolations of the attachment-line similarity solution for over half a century, but this approach is unlikely to deliver accurate predictions of the effect of changes in the attachment line flow on profile drag. An experimental study has been carried out to explore the nature of the turbulent flow in the vicinity of a highly swept swept attachment line and has revealed a quite complex, non-monotonic development of the momentum thickness in this region. It has also revealed lower levels of twist in the boundary layer velocity profiles than anticipated from the highly curved character of the inviscid flow streamlines. These observations have prompted an alternative approach to the modelling of the flow in this region which not only successfully eliminates the lack of robustness in the swept-tapered equations but which also matches the experimental results to within ±5%

Incompressible turbulent flow at the leading edge of swept wings

Due to a singularity in the governing three-dimensional turbulent momentum integral equations at the attachment line, low order infinite-swept and swept-tapered CFD methods employing the viscous-coupling technique have been unable to model attachment line transition or contamination without approximating the development of the boundary layer immediately downstream of a turbulent attachment line. An experimental study was therefore conducted to explore the flow near a turbulent attachment line, which showed considerable differences to the numerical approximation. On the basis of the experimental data; a modification to the governing equations in the attachment line region has been proposed and tested. Comparisons with experimental measurements show that the proposed numerical model is not only able to predict the flow to within ±5%, but it also captures the non-monotonic behaviour of the momentum thickness, in the vicinity of the attachment line, which has not been reported previously