The useful-harmful collar in cemented THR - Do we really do what we are going to do?

We are hereby presenting some purely theoretical mechanical considerations on the danger of the uncorrectly positioned collared femoral stem. DOI: 10.17489/biohun/2009/1/0

Analysis of parameters affecting the shelf life of liquid whole egg

In our measurements we tested the changes in viable cell count in liquid whole eggs. Central complex rotation design was used in planning our experiments, and response surface method (RSM) was applied to analyze the effect of each parameter (pH, storage temperature, storage time and preservative content) on the viable cell count. Based on our measurements, in addition to the storage time, the pH value and storage temperature of liquid egg samples significantly affect (p < 0.01) the viable cell count, but any inhibitory effect of preservatives (Na benzoate, K sorbate mixture) on microbial growth could not be clearly detected. Using the secondary polynomial model which was adjusted to our data, the measurements were defined very well; therefore it is hoped that our results will afford real help in estimation of the microbiological condition of liquid whole egg products which are preserved by various methods

Technical note on the validation of a semi-automated image analysis software application for estrogen and progesterone receptor detection in breast cancer

<p>Abstract</p> <p>Background</p> <p>The immunohistochemical detection of estrogen (ER) and progesterone (PR) receptors in breast cancer is routinely used for prognostic and predictive testing. Whole slide digitalization supported by dedicated software tools allows quantization of the image objects (e.g. cell membrane, nuclei) and an unbiased analysis of immunostaining results. Validation studies of image analysis applications for the detection of ER and PR in breast cancer specimens provided strong concordance between the pathologist's manual assessment of slides and scoring performed using different software applications.</p> <p>Methods</p> <p>The effectiveness of two connected semi-automated image analysis software (<it>NuclearQuant </it>v. 1.13 application for <it>Pannoramic</it>™ <it>Viewer </it>v. 1.14) for determination of ER and PR status in formalin-fixed paraffin embedded breast cancer specimens immunostained with the automated Leica Bond Max system was studied. First the detection algorithm was calibrated to the scores provided an independent assessors (pathologist), using selected areas from 38 small digital slides (created from 16 cases) containing a mean number of 195 cells. Each cell was manually marked and scored according to the Allred-system combining frequency and intensity scores. The performance of the calibrated algorithm was tested on 16 cases (14 invasive ductal carcinoma, 2 invasive lobular carcinoma) against the pathologist's manual scoring of digital slides.</p> <p>Results</p> <p>The detection was calibrated to 87 percent object detection agreement and almost perfect Total Score agreement (Cohen's kappa 0.859, quadratic weighted kappa 0.986) from slight or moderate agreement at the start of the study, using the un-calibrated algorithm. The performance of the application was tested against the pathologist's manual scoring of digital slides on 53 regions of interest of 16 ER and PR slides covering all positivity ranges, and the quadratic weighted kappa provided almost perfect agreement (κ = 0.981) among the two scoring schemes.</p> <p>Conclusions</p> <p><it>NuclearQuant </it>v. 1.13 application for <it>Pannoramic</it>™ <it>Viewer </it>v. 1.14 software application proved to be a reliable image analysis tool for pathologists testing ER and PR status in breast cancer.</p

An easy to use device for lubricity examination

The stability of surgical metals implanted into patients is affected by human extracellular fluidthat can enter between different components of the implant system. However data are missingon the lubricity properties of different human fluids/tissues. In our article we would like to presentan easy and inexpensive device to perform and standardize implant lubricity measurements. DOI: 10.17489/biohun/2009/2/0