Investigation of Spatial Distribution of Radiocesium in a Paddy Field as a Potential Sink

Surface soils, under various land uses, were contaminated by radionuclides that were released by the Fukushima Daiichi Nuclear Power Plant accident. Because paddy fields are one of the main land uses in Japan, we investigated the spatial distribution of radiocesium and the influence of irrigation water in a paddy field during cultivation. Soil core samples collected at a paddy field in Fukushima showed that plowing had disturbed the original depth distribution of radiocesium. The horizontal distribution of radiocesium did not show any evidence for significant influence of radiocesium from irrigation water, and its accumulation within the paddy field, since the original amount of radiocesium was much larger than was added into the paddy field by irrigation water. However, it is possible that rainfall significantly increases the loading of radiocesium.published_or_final_versio

Baseline for ostracod-based northwestern Pacific and Indo-Pacific shallow-marine paleoenvironmental reconstructions: ecological modeling of species distributions

Fossil ostracods have been widely used for Quaternary paleoenvironmental reconstructions, especially in marginal marine environments (e.g., for water depth, temperature, salinity, oxygen levels, pollution). But our knowledge of indicator species autoecology, the base of paleoenvironmental reconstructions, remains limited and commonly lacks robust statistical support and comprehensive comparison with environmental data. We analyzed marginal marine ostracod taxa at 52 sites in Hong Kong for which comprehensive environmental data are available. We applied linear regression models to reveal relationships between species distribution and environmental factors for 18 common taxa (mainly species, a few genera) in our Hong Kong dataset and identified indicator species of environmental parameters. For example, Sinocytheridea impressa, a widely distributed euryhaline species throughout the East and South China Sea and the Indo-Pacific, indicates eutrophication and bottom-water hypoxia. Neomonoceratina delicata, a widely known species from nearshore and estuarine environments in the East and South China Sea and the Indo-Pacific, indicates heavy metal pollution and increased turbidity. The 18 taxa used for this study are widely distributed geographically and divided into the following groups: widespread (throughout the northwestern Pacific and Indo-Pacific regions), temperate (South China Sea to Russia (Sea of Japan coast) and Japan), subtropical (Indo-Pacific to the East China Sea), tropical (Indo-Pacific and South China Sea), and globally distributed. With statistical support from ecological modeling and comprehensive environmental data, these results provide a robust baseline for ostracod-based Quaternary–Anthropocene paleoenvironmental reconstructions in the tropical–extratropical northwestern Pacific and Indo-Pacific. Highlights. We provide a robust baseline for ostracod-based (microscopic Arthropods) paleoenvironmental reconstructions from Quaternary and Anthropocene marginal marine sediments. The studied species have wide distributions over the tropics and extratropics of the northwestern Pacific and Indo-Pacific. Ecological modeling has established ostracod species as reliable indicators for paleoenvironmental reconstructions.</ol

Shallow marine ostracode distribution in Hong Kong

Oral PresentationHong Kong is one of the largest and most rapidly developing cities in Asia. It is known that the marine ecosystems of Hong Kong have been seriously influenced by a variety of anthropogenic factors, including eutrophication, bottom trawling, coastal reclamation, pollution, etc. Crustacean ostracodes are known to be sensitive to such environmental degradation. However, basic information of ostracode distribution is limited in Hong Kong. Here we investigated spatial distribution of modern ostracode assemblages in grab samples. Preliminary results obtained from 55 sites covering most areas of Hong Kong …postprin

Sequence and structural determinants of human APOBEC3H deaminase and anti-HIV-1 activities

Background: Human APOBEC3H (A3H) belongs to the A3 family of host restriction factors, which are cytidine deaminases that catalyze conversion of deoxycytidine to deoxyuridine in single-stranded DNA. A3 proteins contain either one (A3A, A3C, A3H) or two (A3B, A3D, A3F, A3G) Zn-binding domains. A3H has seven haplotypes (I-VII) that exhibit diverse biological phenotypes and geographical distribution in the human population. Its single Zn-coordinating deaminase domain belongs to a phylogenetic cluster (Z3) that is different from the Z1- and Z2-type domains in other human A3 proteins. A3H HapII, unlike A3A or A3C, has potent activity against HIV-1. Here, we sought to identify the determinants of A3H HapII deaminase and antiviral activities, using site-directed sequence- and structure-guided mutagenesis together with cell-based, biochemical, and HIV-1 infectivity assays. Results: We have constructed a homology model of A3H HapII, which is similar to the known structures of other A3 proteins. The model revealed a large cluster of basic residues (not present in A3A or A3C) that are likely to be involved in nucleic acid binding. Indeed, RNase A pretreatment of 293T cell lysates expressing A3H was shown to be required for detection of deaminase activity, indicating that interaction with cellular RNAs inhibits A3H catalytic function. Similar observations have been made with A3G. Analysis of A3H deaminase substrate specificity demonstrated that a 5" T adjacent to the catalytic C is preferred. Changing the putative nucleic acid binding residues identified by the model resulted in reduction or abrogation of enzymatic activity, while substituting Z3-specific residues in A3H to the corresponding residues in other A3 proteins did not affect enzyme function. As shown for A3G and A3F, some A3H mutants were defective in catalysis, but retained antiviral activity against HIV-1vif (-) virions. Furthermore, endogenous reverse transcription assays demonstrated that the E56A catalytic mutant inhibits HIV-1 DNA synthesis, although not as efficiently as wild type. Conclusions: The molecular and biological activities of A3H are more similar to those of the double-domain A3 proteins than to those of A3A or A3C. Importantly, A3H appears to use both deaminase-dependent and -independent mechanisms to target reverse transcription and restrict HIV-1 replication

Dynamics of Simple Balancing Models with State Dependent Switching Control

Time-delayed control in a balancing problem may be a nonsmooth function for a variety of reasons. In this paper we study a simple model of the control of an inverted pendulum by either a connected movable cart or an applied torque for which the control is turned off when the pendulum is located within certain regions of phase space. Without applying a small angle approximation for deviations about the vertical position, we see structurally stable periodic orbits which may be attracting or repelling. Due to the nonsmooth nature of the control, these periodic orbits are born in various discontinuity-induced bifurcations. Also we show that a coincidence of switching events can produce complicated periodic and aperiodic solutions.Comment: 36 pages, 12 figure

Studies of ultra-intense laser plasma interactions for fast ignition

Copyright 2000 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Physics of Plasmas, 7(5), 2014-2022, 2000 and may be found at http://dx.doi.org/10.1063/1.87402

APOBEC3G and APOBEC3F Require an Endogenous Cofactor to Block HIV-1 Replication

APOBEC3G (A3G)/APOBEC3F (A3F) are two members of APOBEC3 cytidine deaminase subfamily. Although they potently inhibit the replication of vif-deficient HIV-1, this mechanism is still poorly understood. Initially, A3G/A3F were thought to catalyze C-to-U transitions on the minus-strand viral cDNAs during reverse transcription to disrupt the viral life cycle. Recently, it was found more likely that A3G/A3F directly interrupts viral reverse transcription or integration. In addition, A3G/A3F are both found in the high-molecular-mass complex in immortalized cell lines, where they interact with a number of different cellular proteins. However, there has been no evidence to prove that these interactions are required for A3G/A3F function. Here, we studied A3G/A3F-restricted HIV-1 replication in six different human T cell lines by infecting them with wild-type or vif-deficient HIV-1. Interestingly, in a CEM-derived cell line CEM-T4, which expresses high levels of A3G/A3F proteins, the vif-deficient virus replicated as equally well as the wild-type virus, suggesting that these endogenous antiretroviral genes lost anti-HIV activities. It was confirmed that these A3G/A3F genes do not contain any mutation and are functionally normal. Consistently, overexpression of exogenous A3G/A3F in CEM-T4 cells still failed to restore their anti-HIV activities. However, this activity could be restored if CEM-T4 cells were fused to 293T cells to form heterokaryons. These results demonstrate that CEM-T4 cells lack a cellular cofactor, which is critical for A3G/A3F anti-HIV activity. We propose that a further study of this novel factor will provide another strategy for a complete understanding of the A3G/A3F antiretroviral mechanism

Indonesian Throughflow drove Australian climate form humid Pliocene to arid Pleistocene

Late Miocene to mid-Pleistocene sedimentary proxy records reveal that northwest Australia underwent an abrupt transition from dry to humid climate conditions at 5.5 million years (Ma), likely receiving year-round rainfall, but after ~3.3 Ma, climate shifted toward an increasingly seasonal precipitation regime. The progressive constriction of the Indonesian Throughflow likely decreased continental humidity and transferred control of northwest Australian climate from the Pacific to the Indian Ocean, leading to drier conditions punctuated by monsoonal precipitation. The northwest dust pathway and fully established seasonal and orbitally controlled precipitation were in place by ~2.4 Ma, well after the intensification of Northern Hemisphere glaciation. The transition from humid to arid conditions was driven by changes in Pacific and Indian Ocean circulation and regional atmospheric moisture transport, influenced by the emerging Maritime Continent. We conclude that the Maritime Continent is the switchboard modulating teleconnections between tropical and high-latitude climate systems.published_or_final_versio