Transcriptomic-metabolomic reprogramming in EGFR-mutant NSCLC early adaptive drug escape linking TGFβ2-bioenergetics-mitochondrial priming.

The impact of EGFR-mutant NSCLC precision therapy is limited by acquired resistance despite initial excellent response. Classic studies of EGFR-mutant clinical resistance to precision therapy were based on tumor rebiopsies late during clinical tumor progression on therapy. Here, we characterized a novel non-mutational early adaptive drug-escape in EGFR-mutant lung tumor cells only days after therapy initiation, that is MET-independent. The drug-escape cell states were analyzed by integrated transcriptomic and metabolomics profiling uncovering a central role for autocrine TGFβ2 in mediating cellular plasticity through profound cellular adaptive Omics reprogramming, with common mechanistic link to prosurvival mitochondrial priming. Cells undergoing early adaptive drug escape are in proliferative-metabolic quiescent, with enhanced EMT-ness and stem cell signaling, exhibiting global bioenergetics suppression including reverse Warburg, and are susceptible to glutamine deprivation and TGFβ2 inhibition. Our study further supports a preemptive therapeutic targeting of bioenergetics and mitochondrial priming to impact early drug-escape emergence using EGFR precision inhibitor combined with broad BH3-mimetic to interrupt BCL-2/BCL-xL together, but not BCL-2 alone

Designing Learning Analytics for Effective Learning Through Problem-Based Learning

This paper describes the development of a learning analytic course for a post-graduate programme at the authors’ university for adult learning. The analytics course is very timely, given the proliferation of Big Data and the need for industries to embrace analytics to examine and solve a range of business problems using data analytics. This is an educational pedagogy and practice paper to design and develop Learning Analytics using Problem-Based Learning (PBL). The contribution of this paper is to help educators in the community who are keen to design and develop similar courses to have a better understanding of data generation, assessment writing, team formation, and challenges faced. The paper will examine and describe the use of the PBL approach to teach learning analytics. The PBL is adopted as it demonstrates a learning design that integrates industry experience with practice-based learning within the classroom. As an approach, it equips students with the ability to solve complex problems through context-driven inquiries. The course has been well-received by students and this paper will discuss the strengths of this approach. Keywords: course planning and development, learning analytics, problem-based learning, course delivery, assessment development DOI: 10.7176/JEP/11-18-03 Publication date:June 30th 202

Removal of chromium from a local Chrome-tannery Wastewater by sodium sulfite reduction coupled with alkali precipitation

The technical feasibility of sodium sulfite reduction coupled with alkali precipitation for the removal of chromium for a local chrome-tannery effluent was evaluated. Initially, the combined reduction-precipitation process was tried using a synthetic solution of pure hexavalent chromium. Independent effects of operating variables (reaction time, pH and sulfite dose) for chromium removal were explored. Results showed that 20 minute-reaction time was needed to attain maximum reduction efficiency. In addition, Cr(VI) reduction efficiency increases with sulfite dose but decreases with pH. Maximum Cr(VI) reduction efficiency (100% Cr(VI) reduction) was attained at pH 1.0 and 2 g/L, sulfite dose. For alkali precipitation, maximum residual total chromium (1.6 ppm) and minimum residual Cr(VI) (0.023 ppm) concentrations were attained at a pH range of 8 to 12. The treated synthetic solution completed with the DAO 35 limit of 0.1ppm for Cr(VI), however, the total chromium concentration was still above the 0.5ppm standard. Using actual chrome-tannery effluent, results of sulfite reduction experiments showed that a maximum of 50% Cr(VI) reduction efficiency was attained at pH 0.5 and sulfite dose of 15mg/L. alkali precipitation results showed that 100% removal of Cr(VI) (or 0ppm residual Cr(VI)) can be attained at pH 8 whether the actual chrome wastewater underwent sulfite reduction prior to alkali precipitation or not. Respective residual total chromium of 12ppm and 14ppm were attained for combined reduction-precipitation process and for alkali precipitation process only. The treated actual chrome wastewater complied with the DAO 35 limit of 0.1ppm for Cr(VI), however, the total chromium concentration was still above the 0.5ppm standard. The treatment cost per cubic meter of wastewater was estimated at Php 36.50 and Php 334.50 for sulfite reduction and alkali precipitation respectively

Genetics of decayed sexual traits in a parasitoid wasp with endosymbiont-induced asexuality.

Trait decay may occur when selective pressures shift, owing to changes in environment or life style, rendering formerly adaptive traits non-functional or even maladaptive. It remains largely unknown if such decay would stem from multiple mutations with small effects or rather involve few loci with major phenotypic effects. Here, we investigate the decay of female sexual traits, and the genetic causes thereof, in a transition from haplodiploid sexual reproduction to endosymbiont-induced asexual reproduction in the parasitoid wasp Asobara japonica. We take advantage of the fact that asexual females cured of their endosymbionts produce sons instead of daughters, and that these sons can be crossed with sexual females. By combining behavioral experiments with crosses designed to introgress alleles from the asexual into the sexual genome, we found that sexual attractiveness, mating, egg fertilization and plastic adjustment of offspring sex ratio (in response to variation in local mate competition) are decayed in asexual A. japonica females. Furthermore, introgression experiments revealed that the propensity for cured asexual females to produce only sons (because of decayed sexual attractiveness, mating behavior and/or egg fertilization) is likely caused by recessive genetic effects at a single locus. Recessive effects were also found to cause decay of plastic sex-ratio adjustment under variable levels of local mate competition. Our results suggest that few recessive mutations drive decay of female sexual traits, at least in asexual species deriving from haplodiploid sexual ancestors

Patient Concerns Inventory for head and neck cancer: Brazilian cultural adaptation

Objective: The purpose of this study was to translate, culturally validate and evaluate the Patients Concerns Inventory - Head and Neck (PCI- H&N) in a consecutive series of Brazilian patients. Methods: This study included adult patients treated for upper aerodigestive tract (UADT) cancer. The translation and cultural adaptation of the PCI–H&N followed internationally accepted guidelines and included a pretest sample of patients that completed the first Brazilian-Portuguese version of the PCI. The use/feasibility/acceptability of the PCI was tested subsequently in a consecutive series of UADT cancer patients that completed the final Brazilian-Portuguese version of the PCI and a Brazilian-Portuguese version of the University of Washington Quality of Life Questionnaire (UW-QOL). Associations between physical and socio emotional composite scores from the UW-QOL and PCI were analyzed. Results: Twenty patients participated in the pretest survey (translation and cultural adaptation process), and 84 patients were analysed in the cultural validation study. Issues most selected were: fear of cancer returning, dry mouth, chewing/eating, speech/voice/being understood, swallowing, dental health/teeth, anxiety, fatigue/tiredness, taste and fear of adverse events. The three specialists most selected were speech therapist, dentist and psychologist. Statistically significant relationships between PCI and UW-QOL were found. Conclusions: The translation and cultural adaptation of the PCI for Brazilian-Portuguese language was successful, and the results demonstrate its feasibility and usefulness, making this a valuable tool for use within the Brazilian H&N cancer population

Intertextuality : a residency project at Lingnan University 2013 = 互為文本

The intertextual works articulate their meanings through the process of creation but not through the artists alone. The significances and meanings of the artworks are produced by the copying and referencing of texts that already exist. Creators of these \u27new\u27 works forgo the identity as an author, the tracing of originality and the following of reading habits and traditions are to be violated. The process of reading, deciphering and judging of the artworks injects new meanings into the original texts written by the old masters; any blockages or fragmentation that happens in the process will further promote the conceptualisation and production of new creations. The exhibition emerges from and transcends two projects - A Residency Project at Lingnan University 2013 and Painting On & On. Painting On & On is an initiative started in Spring 2011 by Hong Kong painters; there were exhibitions and a series of discussions with various motifs being organised throughout these two years. I invited Ivy Ma and Michelle Lee Ho Wing to join this exhibition entitled Intertextuality initiated by me, together with the twenty-four students in my class from the Artists\u27 Residency programme at Lingnan University. The artworks to be shown will respond to the theme, and Jeannie Wu is my co-curator for this exhibition. 一件作品的意念並非固定不變的。我們作為觀眾，在欣賞一件作品時受到很多多因素影響，包括觀眾的知識、觀賞時的客觀環境、及因該作品而產生的相關評論等。作為創作者，要逼使觀眾完全按照自己的理念來完整地理解作品可說是徒勞無功; 倒過來說我們面對前人作品時，亦只能從我們有限的認知當中去接近其理念，但當中總存在差異。這些延異，在當代環境中不但沒有成為創作的阻力，反而被視為一種生產力。創作人以及觀眾都可以透過引用、暗示、甚至破壞重組的方式來創作。這次展覽的作品都具有這種互文性 (Intertextuality)。 這類型互文性的作品，發言的不是藝術家，而是創作活動本身。創作人放棄了作者的位置，選擇在眾多文本之間進行抄寫的活動，衍生出新的作品。這些作品賴以存在的不是作者，而是自由選用的文本汪洋。觀眾有時要違反原有的文化與閱讀習慣，不按明確的規則或模式來閱讀。但這並不代表沒有了判斷作品的方法，而是判斷落入繁雜的關係網中。閱讀的過程會因與原來文本的斷裂、或因繁雜的關係網的無窮無盡感到障礙，但同時這些障礙正是令新作品形成生產力，甚至對原本的前人作品加上新的意義與詮釋。 是次展覽結合了「繪畫大道中(四)」及「嶺南大學藝術家駐場」兩個計劃。「繪畫大道中」是一群香港畫家在2011年春天發起，至今兩年以不同主題籌備一系列討論及展覽。這次以「互為文本」為主題，召集了馬琼珠、李可穎、連同本人周俊輝三位藝術家，加上「嶺南大學藝術家駐場計劃」二十四位學生對這主題的回應作品，及胡津如與本人共同策展。https://commons.ln.edu.hk/vs_artist_catalog/1011/thumbnail.jp

INTEREST IN MATHEMATICS AND ACADEMIC PERFORMANCE OF PRE-SERVICE TEACHERS IN A PUBLIC HIGHER EDUCATION INSTITUTION

This study assessed the relationship between math interest and academic performance of the first-year pre-service teachers enrolled in the Bachelor of Secondary Education Major in Mathematics at Talisay City College, Cebu, Philippines, for the school year 2022-2023 using a descriptive-correlational design. The respondents were identified using simple random sampling. They were asked to answer the questionnaire assessing their math interest, while their performance in math was assessed using their grades in History of Mathematics (MM1) and Fundamentals of Mathematics (Math 1). The research tool was validated using content and face validity. The data gathered were treated using frequency count, percentage, weighted mean, standard deviation, and Pearson Product-Moment Correlation Coefficient. The results revealed that the respondents had a high interest in math. Moreover, they exhibited good performance in MM1 and Math 1. However, no significant relationship was found between math interest and the respondents' MM1 and Math 1 performance. Thus, it is recommended that instructors utilize strategies that enhance students’ interest in math and provide math-related activities that could improve students’ engagement and performance.  Article visualizations

Talking dictionary

Abstract onlyThis study focuses of the Talking Dictionary System of the CPU Library, to change it from a manual system to an automated one. It covers the all types and categories of the Dictionary includes, Talking Koi Encyclopedia and Tagalog, Visayan and Ilokano Language in CD.Includes bibliographical referencesBachelor of Science in Computer Scienc

Practical nutritional recovery strategies for elite soccer players when limited time separates repeated matches

Specific guidelines that aim to facilitate the recovery of soccer players from the demands of training and a congested fixture schedule are lacking; especially in relation to evidence-based nutritional recommendations. The importance of repeated high level performance and injury avoidance while addressing the challenges of fixture scheduling, travel to away venues, and training commitments requires a strategic and practically feasible method of implementing specific nutritional strategies. Here we present evidence-based guidelines regarding nutritional recovery strategies within the context of soccer. An emphasis is placed on providing practically applicable guidelines for facilitation of recovery when multiple matches are played within a short period of time (i.e. 48 h). Following match-play, the restoration of liver and muscle glycogen stores (via consumption of ~1.2 gkg-1h-1 of carbohydrate) and augmentation of protein synthesis (via ~40 g of protein) should be prioritised in the first 20 minutes of recovery. Daily intakes of 6-10 gkg-1 body mass of carbohydrate are recommended when limited time separates repeated matches while daily protein intakes of >1.5 gkg-1 body mass should be targeted; possibly in the form of multiple smaller feedings (e.g., 6 x 20-40 g). At least 150% of the body mass lost during exercise should be consumed within 1 h and electrolytes added such that fluid losses are ameliorated. Strategic use of protein, leucine, creatine, polyphenols and omega-3 supplements could also offer practical means of enhancing post-match recovery. Keywords: soccer, nutrition, recovery, polyphenols, omega-3, creatine, fixture, congestio