Efectos de la herbivoría de peces sobre las praderas marinas de Guang-guang, ciudad de Mati, Filipinas

This study provides information on the effects of fish grazing on a seagrass bed that was dominated by Thalassia hemprichii in Guang-guang, Dahican, Mati City, Davao Oriental, Philippines. We tested the assumption that herbivore exclusion keeps the growth of seagrass shoots up to a minimum length. Fish visual census was used to count the abundance of fish in the area during high tide at a distance of 5 m away from the treatment cages with a 15 m transect length. Among all species, Siganus fuscescens was the most abundant (499) followed by S. guttatus (153), while Thalassoma jansenii was the less abundant (13). The result of the weekly fish count in the seagrass bed showed highly significant variations in the number and census of species present in the area (df = 11, MS = 2.88, F = 30.10, p < 0.001). Analysis of shoot length measurement in treatment cages also exhibited highly significant differences throughout the sampling period (df = 11, MS = 726.71, F = 1,633.10, p < 0.001). Comparison between initial and final shoot counts also showed significant differences, with a higher shoot count in the full cage (X2 = 8, df = 2, p = 0.018; FC > PC > OP). This study raises clear evidence to support the assumption that herbivore exclusion positively influences the growth of T. hemprichii in this area.Este estudio proporciona información sobre los efectos del forrajeo de peces en un lecho de pastos marinos dominado por Thalassia hemprichii en Guang-guang, Dahican, ciudad de Mati, Davao Oriental, Filipinas. Se probó la suposición de que la exclusión de herbívoros mantiene el crecimiento de los brotes de pastos marinos hasta una longitud mínima. Se utilizó el censo visual de peces para contar la abundancia de peces en el área durante la marea alta a una distancia de 5 m de las jaulas de tratamiento con una longitud de transectas de 15 m. Entre todas las especies, Siganus fuscescens fue la más abundante (499) seguida de S. guttatus (153), mientras que Thalassoma jansenii fue la menos abundante (13). El resultado del conteo semanal de peces en el lecho de fanerógamas marinas mostró variaciones altamente significativas en el número y censo de especies presentes en el área (df = 11, MS = 2,88, F = 30,10, p < 0.001). El análisis de la medición de la longitud de los brotes en las jaulas de tratamiento también mostró diferencias muy significativas a lo largo del período de muestreo (df = 11, MS = 726,71, F = 1.633,10, p < 0,001). La comparación entre el conteo de brotes inicial y final también mostró diferencias significativas, con un mayor conteo de brotes en la jaula completa (X2 = 8, df = 2, p = 0,018; FC > PC > OP). Este estudio presenta evidencia clara para apoyar la suposición de que la exclusión de herbívoros influye positivamente en el crecimiento de T. hemprichii en esta área

LEVEL OF STRESS AND COPING MECHANISMS OF COLLEGE STUDENTS IN THE ONLINE DISTANCE LEARNING

This research aimed to investigate the level of stress and coping mechanisms of the College of Education (CED) students at Notre Dame of Midsayap College (NDMC) in online distance learning. This study also aimed to determine if there is a significant difference in the level of stress of the respondents when grouped according to sex and age. A descriptive research design was undertaken to assess the respondents' level of stress and coping mechanisms. The data were subjected to appropriate statistical tools utilized in the study such as frequency count, percentage distribution, weighted mean, and one-way ANOVA.  Based on the major findings of the study, it can be concluded that students experience stress during their online distance learning brought upon by the COVID-19 pandemic. Bad internet connection, financial problems, disturbances in academic life, improper learning environment, tiredness, and irritation in performing their schoolwork are the significant causes of the stress of the students in dealing with their online distance learning. It was also found that they have different coping mechanisms in dealing with stress in their online distance learning. Such coping mechanisms were praying or meditating, creating a conducive learning environment, thinking positively, and playing online games. It was determined that male respondents experienced more stress in online distance learning than female respondents. It was also noted that younger respondents experience more stress than older respondents.  Article visualizations

ING116070: a study of the pharmacokinetics and antiviral activity of dolutegravir in cerebrospinal fluid in HIV-1-infected, antiretroviral therapy-naive subjects.

BackgroundDolutegravir (DTG), a once-daily, human immunodeficiency virus type 1 (HIV-1) integrase inhibitor, was evaluated for distribution and antiviral activity in cerebrospinal fluid (CSF).MethodsING116070 is an ongoing, single-arm, open-label, multicenter study in antiretroviral therapy-naive, HIV-1-infected adults. Subjects received DTG (50 mg) plus abacavir/lamivudine (600/300 mg) once daily. The CSF and plasma (total and unbound) DTG concentrations were measured at weeks 2 and 16. The HIV-1 RNA levels were measured in CSF at baseline and weeks 2 and 16 and in plasma at baseline and weeks 2, 4, 8, 12, and 16.ResultsThirteen white men enrolled in the study; 2 withdrew prematurely, 1 because of a non-drug-related serious adverse event (pharyngitis) and 1 because of lack of treatment efficacy. The median DTG concentrations in CSF were 18 ng/mL (range, 4-23 ng/mL) at week 2 and 13 ng/mL (4-18 ng/mL) at week 16. Ratios of DTG CSF to total plasma concentration were similar to the unbound fraction of DTG in plasma. Median changes from baseline in CSF (n = 11) and plasma (n = 12) HIV-1 RNA were -3.42 and -3.04 log10 copies/mL, respectively. Nine of 11 subjects (82%) had plasma and CSF HIV-1 RNA levels <50 copies/mL and 10 of 11 (91%) had CSF HIV-1 RNA levels <2 copies/mL at week 16.ConclusionsThe DTG concentrations in CSF were similar to unbound plasma concentrations and exceeded the in vitro 50% inhibitory concentration for wild-type HIV (0.2 ng/mL), suggesting that DTG achieves therapeutic concentrations in the central nervous system. The HIV-1 RNA reductions were similar in CSF and plasma. Clinical Trials Registration. NCT01499199

Expression vectors for Neurospora crassa and expression of a bovine preprochymosin cDNA

The filamentous fungi, owing to their ability to secrete high levels of proteins, are attractive organisms for the expression and secretion of heterologous proteins of commercial and medical value. We report the construction of three expression vectors for the production of heterologous proteins in Neurospora crassa and demonstrate their utility by expression of a bovine preprochymosin cDNA and secretion of processed, enzymatically active bovine chymosin

Recent and future trends in synthetic greenhouse gas radiative forcing

Atmospheric measurements show that emissions of hydrofluorocarbons (HFCs) and hydrochlorofluorocarbons are now the primary drivers of the positive growth in synthetic greenhouse gas (SGHG) radiative forcing. We infer recent SGHG emissions and examine the impact of future emissions scenarios, with a particular focus on proposals to reduce HFC use under the Montreal Protocol. If these proposals are implemented, overall SGHG radiative forcing could peak at around 355 mW m[superscript −2] in 2020, before declining by approximately 26% by 2050, despite continued growth of fully fluorinated greenhouse gas emissions. Compared to “no HFC policy” projections, this amounts to a reduction in radiative forcing of between 50 and 240 mW m[superscript −2] by 2050 or a cumulative emissions saving equivalent to 0.5 to 2.8 years of CO2 emissions at current levels. However, more complete reporting of global HFC emissions is required, as less than half of global emissions are currently accounted for.Natural Environment Research Council (Great Britain) (Advanced Research Fellowship NE/I021365/1)United States. National Aeronautics and Space Administration (Upper Atmospheric Research Program Grant NNX11AF17G)United States. National Oceanic and Atmospheric Administratio

Identification of genes regulating migration and invasion using a new model of metastatic prostate cancer

Background: Understanding the complex, multistep process of metastasis remains a major challenge in cancer research. Metastasis models can reveal insights in tumor development and progression and provide tools to test new intervention strategies. Methods: To develop a new cancer metastasis model, we used DU145 human prostate cancer cells and performed repeated rounds of orthotopic prostate injection and selection of subsequent lymph node metastases. Tumor growth, metastasis, cell migration and invasion were analyzed. Microarray analysis was used to identify cell migration- and cancer-related genes correlating with metastasis. Selected genes were silenced using siRNA, and their roles in cell migration and invasion were determined in transwell migration and Matrigel invasion assays. Results: Our in vivo cycling strategy created cell lines with dramatically increased tumorigenesis and increased ability to colonize lymph nodes (DU145LN1-LN4). Prostate tumor xenografts displayed increased vascularization, enlarged podoplanin-positive lymphatic vessels and invasive margins. Microarray analysis revealed gene expression profiles that correlated with metastatic potential. Using gene network analysis we selected 3 significantly upregulated cell movement and cancer related genes for further analysis: EPCAM (epithelial cell adhesion molecule), ITGB4 (integrin β4) and PLAU (urokinase-type plasminogen activator (uPA)). These genes all showed increased protein expression in the more metastatic DU145-LN4 cells compared to the parental DU145. SiRNA knockdown of EpCAM, integrin-β4 or uPA all significantly reduced cell migration in DU145-LN4 cells. In contrast, only uPA siRNA inhibited cell invasion into Matrigel. This role of uPA in cell invasion was confirmed using the uPA inhibitors, amiloride and UK122. Conclusions: Our approach has identified genes required for the migration and invasion of metastatic tumor cells, and we propose that our new in vivo model system will be a powerful tool to interrogate the metastatic cascade in prostate cancer

Ventricular-vascular coupling is predictive of adverse clinical outcome in paediatric pulmonary arterial hypertension

AIMS: Ventricular-vascular coupling, the ratio between the right ventricle's contractile state (Ees) and its afterload (Ea), may be a useful metric in the management of paediatric pulmonary arterial hypertension (PAH). In this study we assess the prognostic capacity of the ventricular-vascular coupling ratio (Ees/Ea) derived using right ventricular (RV) pressure alone in children with PAH. METHODS: One hundred and thirty paediatric patients who were diagnosed with PAH via right heart catheterisation were retrospectively reviewed over a 10-year period. Maximum RV isovolumic pressure and end-systolic pressure were estimated using two single-beat methods from Takeuchi et al (Ees/Ea_(Takeuchi)) and from Kind et al (Ees/Ea_(Kind)) and used with an estimate of end-systolic pressure to compute ventricular-vascular coupling from pressure alone. Patients were identified as either idiopathic/hereditary PAH or associated PAH (IPAH/HPAH and APAH, respectively). Haemodynamic data, clinical functional class and clinical worsening outcomes-separated into soft (mild) and hard (severe) event categories-were assessed. Adverse soft events included functional class worsening, syncopal event, hospitalisation due to a proportional hazard-related event and haemoptysis. Hard events included death, transplantation, initiation of prostanoid therapy and hospitalisation for atrial septostomy and Pott's shunt. Cox proportional hazard modelling was used to assess whether Ees/Ea was predictive of time-to-event. RESULTS: In patients with IPAH/HPAH, Ees/Ea_(Kind) and Ees/Ea_(Takeuchi) were both independently associated with time to hard event (p=0.003 and p=0.001, respectively) and when adjusted for indexed pulmonary vascular resistance (p=0.032 and p=0.013, respectively). Neither Ees/Ea_(Kind) nor Ees/Ea_(Takeuchi) were associated with time to soft event. In patients with APAH, neither Ees/Ea_(Kind) nor Ees/Ea_(Takeuchi) were associated with time to hard event or soft event. CONCLUSIONS: Ees/Ea derived from pressure alone is a strong independent predictor of adverse outcome and could be a potential powerful prognostic tool for paediatric PAH

Survival Differences in Pediatric Pulmonary Arterial Hypertension Clues to a Better Understanding of Outcome and Optimal Treatment Strategies

ObjectivesIn order to describe survival and treatment strategies in pediatric pulmonary arterial hypertension (PAH) in the current era of PAH-targeted drugs and to identify predictors of outcome, we studied uniformly defined contemporary patient cohorts at 3 major referral centers for pediatric PAH (New York [NY], Denver, and the Netherlands [NL]).BackgroundIn pediatric PAH, discrepancies exist in reported survival rates between North American and European patient cohorts, and robust data for long-term treatment effects are lacking.MethodsAccording to uniform inclusion criteria, 275 recently diagnosed consecutive pediatric PAH patients who visited the 3 referral centers between 2000 and 2010 were included.ResultsUnadjusted survival rates differed between the center cohorts (1-, 3-, and 5-year transplantation-free survival rates: 100%, 96%, and 90% for NY; 95%, 87%, and 78% for Denver; and 84%, 71%, and 62% for NL, respectively; p < 0.001). Based on World Health Organization (WHO) functional class and hemodynamic parameters, disease severity at diagnosis differed between the center cohorts. Adjustment for diagnosis, WHO functional class, indexed pulmonary vascular resistance, and pulmonary-to-systemic arterial pressure ratio resolved the observed survival differences. Treatment with PAH-targeted dual and triple therapy during the study period was associated with better survival than treatment with PAH-targeted monotherapy.ConclusionsSurvival rates of pediatric PAH patients differed between 3 major referral centers. This could be explained by differences between the center cohorts in patients’ diagnoses and measures of disease severity, which were identified as important predictors of outcome. In this study, treatment with PAH-targeted combination therapy during the study period was independently associated with improved survival