Metabolic changes in experimental model of metabolic syndrome - induced by high-fructose diet in rats

The global epidemic of metabolic syndrome (MS) correlates with changes in the environment, feeding, behavior and lifestyle, leading to obesity, glucose intolerans, dyslipidemia and elevated cardiovascular risk. AIM: The aim of our study was to develop an experimental model of the MS in rat that imitate the investigated metabolic disorders using high-fructose diet. METHODS: We used two groups: control group (C)- rats, maintained on plain water (n=6); fructose group (FRU)- rats received 12.5% high-fructose corn syrup in drinking water for 12 weeks (n=6). The main markers of metabolic abnormalities (glucose, total cholesterol, triglycerides, uric acid, body and organs weight), the markers of oxidative stress (malondialdehyde (MDA), total thiols) and C-reactive protein (CRP) - inflammatory marker were measured. RESULTS: Our data showed hypercholesterolemia, hyperglycemia, hyperuricemia and significant elevated levels of CRP, MDA, body and organs weight, and inhibited antioxidant defense in fructose- drinking rats. CONCLUSION: The experimental model will support our studies associated with pathophysiology and pharmacology of MS

Teamworking and Concern for Standards as Part of Employability Readiness of UAE Students` Personality

In the time of globalization and technological revolution, we often witness that skillset required to go through life demands are changing dramatically; more digital literacy is required and specific soft skills. The last is a growing concern, especially for growing economies and societies, where involving the local workforce is a challenge. In the United Arab Emirates (UAE), the government places a huge emphasis on Emiratization and puts conditions on employers to include Emirati nationals in the workforce. Preparation for work starts with attitude, raised in the family, affected by the educational system, and includes specific personality factors in specific correlation. This study aims to provide light on the status of employability readiness of the UAE students, specifically team working and concern for standards as factors correlating with employability and shows its correlations.   The last can be considered when updating university programs for preparing the UAE youth to take part in the global workforce. Results showed a strong relationship at the level of significance (α = 0.05) between concern for standards and employability, in addition, there is no effect of the gender variable (male, female) on the student's personality and the degree of his employability

Duration of the patient interval in breast cancer and factors associated with longer delays in low‐and middle‐income countries: A systematic review with meta‐analysis

Objective: Breast cancer survival is lower in low‐ and middle‐income countries (LMICs) partially due to many women being diagnosed with late‐stage disease. The patient interval refers to the time elapsed between the detection of symptoms and the first consultation with a healthcare provider and is considered one of the core indicators for early diagnosis and treatment. The goal of the current research was to conduct a meta‐analysis of the duration of the patient interval in LMICs and investigate the socio‐demographic and socio‐cultural factors related to longer delays in presentation. Methods: We conducted a systematic review with meta‐analysis (pre‐registered protocol CRD42020200752). We searched seven information sources (2009–2022) and included 50 articles reporting the duration of patient intervals for 18,014 breast cancer patients residing in LMICs. Results: The longest patient intervals were reported in studies from the Middle East (3–4 months), followed by South‐East Asia (2 months), Africa (1–2 months), Latin America (1 month), and Eastern Europe (1 month). Older age, not being married, lower socio‐economic status, illiteracy, low knowledge about cancer, disregarding symptoms or not attributing them to cancer, fear, negative beliefs about cancer, and low social support were related to longer delays across most regions. Longer delays were also related to use of alternative medicine in the Middle East, South‐East Asia, and Africa and distrust in the healthcare system in Eastern Europe. Conclusions: There is large variation in the duration of patient intervals across LMICs in different geographical regions. Patient intervals should be reduced and, for this purpose, it is important to explore their determinants taking into account the social, cultural, and economic context.Centro de Investigacion Biomedica en Red de Epidemiologia y Salud PublicaAgencia Estatal de InvestigacionFundacion Cientifica Asociacion Espanola Contra el Cance

TETRANUCLEOTIDE MICROSATELLITES ON THE MAP OF CHROMOSOME 13. THE ANALYSIS OF DISTRIBUTION AND APPLICATION TO THE MARKER'S CREATION

The object of investigation: tetranucleotide repeatitions on the chromosome of 13 of the man. The purpose of the work: the search for the new loci of the tetranucleotide microsatellites on the chromosome 13 and the formation of the new genetic markers on its base. The screening of the ordered clonotheca of chromosome 13 for the content of the consequences of three- and tetranucleotide repeatitions has been performed, and its distribution on the chromosome has been determined. 40 GATG- and GACT-positive cosmides have been localized with the FISH method on the metaphase chromosomes 16 cosmides have been recloned and partially sequenced have been the obtained reclones; 6 markers have been formed; the analysis of the association of the polymorph marker D13S699 (13q14) with shizophrenia has been given (is applied to solving the questions about the controversial paternity). The obtained data can be used in mapping of chromosome 13, in the genome dactiloscopy for identification of a person and in the associative analysis of the definite hereditary diseasesAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio

Biallelic Mutations in TBCD, Encoding the Tubulin Folding Cofactor D, Perturb Microtubule Dynamics and Cause Early-Onset Encephalopathy

Microtubules are dynamic cytoskeletal elements coordinating and supporting a variety of neuronal processes, including cell division, migration, polarity, intracellular trafficking, and signal transduction. Mutations in genes encoding tubulins and microtubule-associated proteins are known to cause neurodevelopmental and neurodegenerative disorders. Growing evidence suggests that altered microtubule dynamics may also underlie or contribute to neurodevelopmental disorders and neurodegeneration. We report that biallelic mutations in TBCD, encoding one of the five co-chaperones required for assembly and disassembly of the αβ-tubulin heterodimer, the structural unit of microtubules, cause a disease with neurodevelopmental and neurodegenerative features characterized by early-onset cortical atrophy, secondary hypomyelination, microcephaly, thin corpus callosum, developmental delay, intellectual disability, seizures, optic atrophy, and spastic quadriplegia. Molecular dynamics simulations predicted long-range and/or local structural perturbations associated with the disease-causing mutations. Biochemical analyses documented variably reduced levels of TBCD, indicating relative instability of mutant proteins, and defective β-tubulin binding in a subset of the tested mutants. Reduced or defective TBCD function resulted in decreased soluble α/β-tubulin levels and accelerated microtubule polymerization in fibroblasts from affected subjects, demonstrating an overall shift toward a more rapidly growing and stable microtubule population. These cells displayed an aberrant mitotic spindle with disorganized, tangle-shaped microtubules and reduced aster formation, which however did not alter appreciably the rate of cell proliferation. Our findings establish that defective TBCD function underlies a recognizable encephalopathy and drives accelerated microtubule polymerization and enhanced microtubule stability, underscoring an additional cause of altered microtubule dynamics with impact on neuronal function and survival in the developing brai