Antifungal activity of essential oil Hyssopus officinalis L. against micopathogen Mycogone perniciosa (Mang)

The most commonly cultivated mushroom species is the Agaricus bisporus Lange (Imb). One of the major pathogenic diseases of the cultivated mushroom in Serbia is Mycogone perniciosa (Mang). Biological control systems are not much used in mushroom cultivation. Medical and aromatic plants have been placed in the focus of intense studies. Pure culture of the M. perniciosa was isolated from infected A. bisporus. The essential oil of Hyssopus officinalis L. is used as a potential antifungal agent. The most abundant components in oil are isopinocamphone (43.29%), pinocamphone (16.79%) and b-pinene (16.31%). Antifungal activity of Hyssop was investigated by the modified microatmosphere method. The minimal inhibitory quantity was 5 μL/mL and a minimal fungicidal quantity was 15-20 μL/mL. There is no report on the use of Hyssop essential oil in mushroom disease.Agaricus bisporus Lange (Imb) je najčešće komercijalno gajena jestiva gljiva Različiti mikroorganizmi gljive, bakterije i virusi su izazivači bolesti u gajilištima šampinjona. Mycogone perniciosa (Mang) je izazivač bolesti poznate pod nazivom vlažni mehur i najčešći uzročnik gubitaka u gajilištima u Srbiji. Biološka kontrola, koja je uspešno primenjivana na nekim poljoprivrednim kulturama, nije korišćena prilikom uzgoja gljiva. Jedna od mogućnosti je primena biljnih sprejova. Lekovite i aromatične vrste biljaka se intenzivno istražuju kao mogući antifungalni agensi. Uzorci obolelih šampinjona su sakupljani u gajilištima u Srbiji. Kulture M. perniciosa su izolovane sa obolelih plodonosnih tela A. bisporus. Korišćeno je etarsko ulje Hyssopus officinalis. Najzastupljenije komponente ulja su izopinokamfon (43.29%) trans-pinokamfon (16.79%) i b-pinen (16.31%). Antifungalna aktivnost etarskog ulja izopa ispitivana je modifikovanom "mikroatmosfera"- metodom. Minimalna inhibitorna količina je bila 5 μL/mL, a minimalna fungicidna količina 15-20 μL/mL. Velik broj preparata je napravljen i primenjen za kontrolisanje oboljenja pečuraka: fungicidi, primena mikrotalasa ili dejstvo nekih antagonističkih bakterija. Dosad nije bilo saopštenja o primeni etarskog ulja izopa protiv izazivača bolesti gajenih gljiva.nul

Morpho-physiological characteristics and interactions of isolates of Mycogone perniciosa (Magnus) Delacr

Mycogone perniciosa (Magnus) Delacr., which causes wet bubble disease of Agaricus bisporus Lange (Imb), results in a considerable crop loss on mushroom farms in Serbia. The isolation and identification of five isolates of M. perniciosa from diseased fruit bodies of white button mushroom from mushroom units in Serbia, Bosnia and Herzegovina and Holland were made. Morpho-physiological characteristics and inter-relationships of the obtained isolates were studied. Macroscopic and microscopic investigations of different zones between colonies of the isolates of M. perniciosa revealed the phenomenon of the hyphal interference between different isolates. The obtained results suggest that hyphal interference could serve as an additional parameter for a more reliable determination of fungal specifity.Mycogone perniciosa (Magnus) Delacr., izazivač oboljenja mokre truleži najčešći je uzročnik gubitaka u gajilištima Agaricus bisporus Lange (Imb) u Srbiji. Izvršena je izolacija i identifikacija 5 izolata M. perniciosa sa obolelih plodonosnih tela šampinjona iz gajilišta u Srbiji, Bosni i Hercegovini i Holandiji. Ispitivane su morfo-fiziološke karakteristike kao i stepen srodnosti proučavanih izolata na osnovu analize međusobnog delovanja kolonija, odnosno korišćenjem fenomena hifalne interferencije. Makroskopska i mikroskopska istraživanja odnosa izolata i dobijeni rezultati ukazuju da hifalna interferencija može predstavljati dodatni parametar u razlikovanju izolata M. perniciosa. Izolati dobijeni iz gajilišta u Srbiji slični su međusobno, kao i izolati iz Holandije koji su pokazali međusobnu sličnost ali se razlikuju od prethodnih. Izolati iz Bosne i Hercegovine razlikovali su se i od srpskih i od holandskih.Projekat ministarstva br. 14304

Effects of agaricus bisporus mushroom extract on honey bees infected with nosema ceranae

Agaricus bisporus water crude extract was tested on honey bees for the first time. The first part of the cage experiment was set for selecting one concentration of the A. bisporus extract. Concentration of 200 µg/g was further tested in the second part of the experiment where bee survival and food consumption were monitored together with Nosema infection level and expression of five genes (abaecin, hymenoptaecin, defensin, apidaecin, and vitellogenin) that were evaluated in bees sampled on days 7 and 15. Survival rate of Nosema-infected bees was significantly greater in groups fed with A. bisporus-enriched syrup compared to those fed with a pure sucrose syrup. Besides, the anti-Nosema effect of A. bisporus extract was greatest when applied from the third day which coincides with the time of infection with N. ceranae. Daily food consumption did not differ between the groups indicating good acceptability and palatability of the extract. A. bisporus extract showed a stimulative effect on four out of five monitored genes. Both anti-Nosema and nutrigenomic effects of A. bisporus extract were observed when supplementation started at the moment of N. ceranae infection or preventively (before or simultaneously with the infection)

Supplementary data for article: Glavinić, U.; Rajković, M.; Vunduk, J.; Vejnović, B.; Stevanović, J.; Milenković, I.; Stanimirović, Z. Effects of Agaricus bisporus Mushroom Extract on Honey Bees Infected with Nosema ceranae. Insects 2021, 12 (10). https://doi.org/10.3390/insects12100915.

Supplementary material for: [https://doi.org/10.3390/insects12100915]Related to published version: [https://vet-erinar.vet.bg.ac.rs/handle/123456789/2234

Supplementary data for article: Čobeljić, B.; Pevec, A.; Turel, I.; Swart, M.; Mitić, D.; Milenković, M.; Marković, I.; Jovanović, M.; Sladić, D.; Jeremić, M.; et al. Synthesis, Characterization, DFT Calculations and Biological Activity of Derivatives of 3-Acetylpyridine and the Zinc(II) Complex with the Condensation Product of 3-Acetylpyridine and Semicarbazide. Inorganica Chimica Acta 2013, 404, 5–12. https://doi.org/10.1016/j.ica.2013.04.017

Supplementary material for: [https://doi.org/10.1016/j.ica.2013.04.017]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1367]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3539

Pozitivan učinak dimetilsulfoksida na hepatoprotektivnu aktivnost volframata

Tungstates and polyoxotungstates have been known to be bioactive compounds for a long time. Based on our previous work about sodium tungstate (ST) and 12-tungstophosphoric acid (WPA) as hepatoprotective agents, we performed a seven week long experiment in rats, using ST and WPA pretreatments in thioacetamide (TAA) induced acute liver necrosis. The possible influences of dimethyl sulfoxide (DMSO), given orally for 3 consecutive days before inducing hepatic lesions, were also investigated. The effects were evaluated by the activity of serum enzymes, oxidative stress parameters, antioxidative defense markers, and histopathology in Wistar rats. The obtained results suggest that oral pretreatment with tungstates, especially ST, in combination with DMSO, before the TAA inducted liver necrosis, could be useful for the prevention of hepatic injury in rats.Volframati i polioksovolframati su decenijama poznati kao biološki aktivna jedinjenja. Kao nastavak prethodnog istraživanja hepatoprotektivnog efekta natrijum volframata (ST) i 12-volframfosforne kiseline (WPA), izveden je eksperiment na Wistar pacovima, koji su pretretirani sa ST i WPA tokom sedam nedelja a nakon tog perioda im je intraperitonealno ubrizgan tioacetamid (TAA) u cilju indukovanja akutne nekroze jetre. Ispitivan je uticaj dimetil sulfoksida (DMSO), koji je primenjivan per os u toku 3 konsekutativna dana pre indukcije hepatične nekroze. Efekti su evaluirani praćenjem aktivnosti serumskih enzima, parametara oksidativnog stresa, antioksidativnog odbrambenog markera, kao i histopatološkom analizom tkiva jetre. Dobijeni rezultati ukazuju da oralni pretretman pacova sa volframatima, pre svega ST, u kombinaciji sa DMSO, može biti koristan u prevenciji nekroze jetre indukovane tioacetamidom

Antifungal activity of essential oil Hyssopus officinalis L. against micopathogen Mycogone perniciosa (Mang)

The most commonly cultivated mushroom species is the Agaricus bisporus Lange (Imb). One of the major pathogenic diseases of the cultivated mushroom in Serbia is Mycogone perniciosa (Mang). Biological control systems are not much used in mushroom cultivation. Medical and aromatic plants have been placed in the focus of intense studies. Pure culture of the M. perniciosa was isolated from infected A. bisporus. The essential oil of Hyssopus officinalis L. is used as a potential antifungal agent. The most abundant components in oil are isopinocamphone (43.29%), pinocamphone (16.79%) and b-pinene (16.31%). Antifungal activity of Hyssop was investigated by the modified microatmosphere method. The minimal inhibitory quantity was 5 μL/mL and a minimal fungicidal quantity was 15-20 μL/mL. There is no report on the use of Hyssop essential oil in mushroom disease.Agaricus bisporus Lange (Imb) je najčešće komercijalno gajena jestiva gljiva Različiti mikroorganizmi gljive, bakterije i virusi su izazivači bolesti u gajilištima šampinjona. Mycogone perniciosa (Mang) je izazivač bolesti poznate pod nazivom vlažni mehur i najčešći uzročnik gubitaka u gajilištima u Srbiji. Biološka kontrola, koja je uspešno primenjivana na nekim poljoprivrednim kulturama, nije korišćena prilikom uzgoja gljiva. Jedna od mogućnosti je primena biljnih sprejova. Lekovite i aromatične vrste biljaka se intenzivno istražuju kao mogući antifungalni agensi. Uzorci obolelih šampinjona su sakupljani u gajilištima u Srbiji. Kulture M. perniciosa su izolovane sa obolelih plodonosnih tela A. bisporus. Korišćeno je etarsko ulje Hyssopus officinalis. Najzastupljenije komponente ulja su izopinokamfon (43.29%) trans-pinokamfon (16.79%) i b-pinen (16.31%). Antifungalna aktivnost etarskog ulja izopa ispitivana je modifikovanom "mikroatmosfera"- metodom. Minimalna inhibitorna količina je bila 5 μL/mL, a minimalna fungicidna količina 15-20 μL/mL. Velik broj preparata je napravljen i primenjen za kontrolisanje oboljenja pečuraka: fungicidi, primena mikrotalasa ili dejstvo nekih antagonističkih bakterija. Dosad nije bilo saopštenja o primeni etarskog ulja izopa protiv izazivača bolesti gajenih gljiva.nul

Morpho-physiological characteristics and interactions of isolates of Mycogone perniciosa (Magnus) Delacr

Mycogone perniciosa (Magnus) Delacr., which causes wet bubble disease of Agaricus bisporus Lange (Imb), results in a considerable crop loss on mushroom farms in Serbia. The isolation and identification of five isolates of M. perniciosa from diseased fruit bodies of white button mushroom from mushroom units in Serbia, Bosnia and Herzegovina and Holland were made. Morpho-physiological characteristics and inter-relationships of the obtained isolates were studied. Macroscopic and microscopic investigations of different zones between colonies of the isolates of M. perniciosa revealed the phenomenon of the hyphal interference between different isolates. The obtained results suggest that hyphal interference could serve as an additional parameter for a more reliable determination of fungal specifity.Mycogone perniciosa (Magnus) Delacr., izazivač oboljenja mokre truleži najčešći je uzročnik gubitaka u gajilištima Agaricus bisporus Lange (Imb) u Srbiji. Izvršena je izolacija i identifikacija 5 izolata M. perniciosa sa obolelih plodonosnih tela šampinjona iz gajilišta u Srbiji, Bosni i Hercegovini i Holandiji. Ispitivane su morfo-fiziološke karakteristike kao i stepen srodnosti proučavanih izolata na osnovu analize međusobnog delovanja kolonija, odnosno korišćenjem fenomena hifalne interferencije. Makroskopska i mikroskopska istraživanja odnosa izolata i dobijeni rezultati ukazuju da hifalna interferencija može predstavljati dodatni parametar u razlikovanju izolata M. perniciosa. Izolati dobijeni iz gajilišta u Srbiji slični su međusobno, kao i izolati iz Holandije koji su pokazali međusobnu sličnost ali se razlikuju od prethodnih. Izolati iz Bosne i Hercegovine razlikovali su se i od srpskih i od holandskih.Projekat ministarstva br. 14304

Supplementary data for article: Čobeljić, B.; Pevec, A.; Turel, I.; Swart, M.; Mitić, D.; Milenković, M.; Marković, I.; Jovanović, M.; Sladić, D.; Jeremić, M.; et al. Synthesis, Characterization, DFT Calculations and Biological Activity of Derivatives of 3-Acetylpyridine and the Zinc(II) Complex with the Condensation Product of 3-Acetylpyridine and Semicarbazide. Inorganica Chimica Acta 2013, 404, 5–12. https://doi.org/10.1016/j.ica.2013.04.017

Supplementary material for: [https://doi.org/10.1016/j.ica.2013.04.017]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1367]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3539

Jedan od faktora koji utiču na kvarenje steriliziranog mlijeka

Termički tretmani koji se upotrebljavaju za sterilizaciju mlijeka ne uništavaju uvijek sve mikroorganizme, naročito ne izvjesne forme spora termorezistentnih bakterija iz familije Bacillaceae i to rodova Bacillus i Clostridium