The antimicrobial evaluation of some biginelli-based azo pyridone dyes

Heterociklična azo jedinjenja predstavljaju značajnu klasnu organskih sintetskih boja. Pored njihovih izuzetnih svojstava za bojenje, heterociklična azo jedinjenja imaju i biološku aktivnost. Mnoge studije pokazuju da azo boje sa heterocikličnim fragmentima u strukturi mogu ispoljavati antibakterijska, antifungalna, antivirusna, antiinflamatorna i antikancerogena svojstva. Rezistencija mikroorganizama na postojeće antimikrobne agense predstavlja jedan odnajvećih problema u svet-skom zdravstvu. Zbog toga je razvijanje novih molekula sa antimikrobnim svojstvima kako značajno, tako i neophodno za globalno očuvanje zdravlja. U ovom radu, ispitivanje antimikrobne aktivnosti dve Biđineli-azo piridonske boje je urađeno pomoću metode difuzije na agarnoj podlozi. Anti-mikrobna svojstva su ispitana prema patogenim sojevima Staphylococcus aureus (Gram-pozitivna bakterija), Escherichia coli (Gram-negativna bakterija) i Candida albicans (oportunistički kvasac). Preliminarni rezultati istraživanja ukazali su na antimikrobni potencijal ispitivanih azo jedinjenja.The heterocyclic azo dyes represent significant synthetic organic colorants. In addition to their exceptional coloration properties, heterocyclic azo compounds exhibit antibacterial, antifungal, an-tiviral, antioxidant, anti-inflammatory and anticancer properties. The antimicrobial resistance is one of the major problems for global public health. Therefore, finding new organic structures with anti-microbial properties represents a challenging research field. In this study, antimicrobial screening of two Biginelli-based azo pyridone dyes has been carried out. The antimicrobial activity of synthe-tized dyes wasstudied against bacteria (Staphylococcus aureus, Escherichia coli) and yeast (Candida albicans), using agar diffusion method. The preliminary research results indicate the antimicrobial potential of the investigated compounds35. Međunarodni kongres o procesnoj industriji - PROCESING ′22 : zbornik radova ; 1-3. jun, Beogra

Comparison of growth parameters of CHO-K1 and CCO cells in culture

Tehnologija životinjskih stanica te mogućnost njihove primjene u industriji i proizvodnji korisnih proizvoda zauzima značajno mjesto u biotehnologiji i srodnim znanstvenim područjima. Cilj rada je utvrditi parametre uzgoja CCO i CHO-K1 stanične linije uz naglasak na potrošnju i nastajanje metabolita u mediju za uzgoj. Istraživan je rast CCO stanica tijekom 9 dana uzgoja u dva različita medija, DMEM (ATCC) + 10% FBSa i D-MEM/F12 (Gibco) + 10% NCS-a. Utvrđen je veći prirast CCO stanica nakon 5 dana uzgoja u DMEM mediju s 10% FBS-a u usporedbi s uzgojem u mediju s 5% FBS-a i 2,5% FBS-a. Tijekom 5 dana uzgoja CCO i CHO-K1 stanica praćen je utrošak metabolita u mediju. Rezultati pokazuju da CCO stanice ne koriste glukozu kao primarni izvor energije. Mogući izvor energije za ove stanice su aminokiseline, naročito glutamin. Također je utvrđeno nakupljanje manje količine laktata kao metaboličkog nusproizvoda. Određivanje metabolita u D-MEM/F12 mediju pokazuje da CHO-K1 stanice koriste glukozu kao glavni izvor energije, pri čemu se laktat nakuplja u većoj količini. To upućuje na nepotpunu razgradnju glukoze. Ionskom izmjenom u mediju za uzgoj CCO stanica određeno je nakupljanje alanina i glicina, kao i potrošnja treonina, serina, metionina, arginina, taurina i glutamina. Određivanje aminokiselina u mediju za uzgoj CHO-K1 stanica pokazuje nakupljanje alanina i ornitina, kao i potrošnju asparaginske kiseline, glutaminske kiseline, glutamina, serina i treonina. Nakupljanje laktata i alanina u mediju za uzgoj CHO-K1 stanica ukazuje na veliku potrošnju glukoze i aminokiselina što predstavlja nesrazmjer u potrošnji metabolita i energetskim potrebama stanica u kulturi.Application of animal cell technology for production of active biological components has an important place in biotechnology. Object of this study was determination of growth parameters of CCO and CHO-K1 animal cells as well as monitoring of medium metabolite consumption and production. This study reports on growth of CCO cell line during 9 days of cultivation in two different media, DMEM (ATCC) + 10% FBS and D-MEM/F12 (Gibco) + 10% NCS. Growth of CCO cell line was found better in DMEM medium with 10% FBS than in medium with 5% or 2,5% FBS. Consumption of medium metabolites was monitored during 5 days of growth of CCO and CHO-K1 cell lines. The results show that CCO cells do not use glucose as a primary energy source. Possible source of energy for these cells may be amino acids, especially glutamine. However accumulation of lactate as metabolic byproduct was also detected. Metabolite detection of D-MEM/F12 medium showed that CHO-K1 cells use glucose as a main source of energy while lactate accumulates as metabolic byproduct. This indicates of incomplete break-down of glucose. Accumulation of alanine and glycine, as well as consumption of treonine, serine, methionine, arginine, taurine and glutamine in medium for CCO cells cultivation was detected by ion-exchange chromatography. The amino acid detection in medium for CHO-K1 cell cultivation showed accumulation of alanine and ornithine, as well as consumption of asparartate, glutamic acid, glutamine, serine and threonine. Accumulation of lactate and alanine in growth medium for CHO-K1 cells is due to significant glucose and amino acids consumption, which represents typical metabolic disfunction in energy requirement of cultured cells

Comparison of growth parameters of CHO-K1 and CCO cells in culture

Tehnologija životinjskih stanica te mogućnost njihove primjene u industriji i proizvodnji korisnih proizvoda zauzima značajno mjesto u biotehnologiji i srodnim znanstvenim područjima. Cilj rada je utvrditi parametre uzgoja CCO i CHO-K1 stanične linije uz naglasak na potrošnju i nastajanje metabolita u mediju za uzgoj. Istraživan je rast CCO stanica tijekom 9 dana uzgoja u dva različita medija, DMEM (ATCC) + 10% FBSa i D-MEM/F12 (Gibco) + 10% NCS-a. Utvrđen je veći prirast CCO stanica nakon 5 dana uzgoja u DMEM mediju s 10% FBS-a u usporedbi s uzgojem u mediju s 5% FBS-a i 2,5% FBS-a. Tijekom 5 dana uzgoja CCO i CHO-K1 stanica praćen je utrošak metabolita u mediju. Rezultati pokazuju da CCO stanice ne koriste glukozu kao primarni izvor energije. Mogući izvor energije za ove stanice su aminokiseline, naročito glutamin. Također je utvrđeno nakupljanje manje količine laktata kao metaboličkog nusproizvoda. Određivanje metabolita u D-MEM/F12 mediju pokazuje da CHO-K1 stanice koriste glukozu kao glavni izvor energije, pri čemu se laktat nakuplja u većoj količini. To upućuje na nepotpunu razgradnju glukoze. Ionskom izmjenom u mediju za uzgoj CCO stanica određeno je nakupljanje alanina i glicina, kao i potrošnja treonina, serina, metionina, arginina, taurina i glutamina. Određivanje aminokiselina u mediju za uzgoj CHO-K1 stanica pokazuje nakupljanje alanina i ornitina, kao i potrošnju asparaginske kiseline, glutaminske kiseline, glutamina, serina i treonina. Nakupljanje laktata i alanina u mediju za uzgoj CHO-K1 stanica ukazuje na veliku potrošnju glukoze i aminokiselina što predstavlja nesrazmjer u potrošnji metabolita i energetskim potrebama stanica u kulturi.Application of animal cell technology for production of active biological components has an important place in biotechnology. Object of this study was determination of growth parameters of CCO and CHO-K1 animal cells as well as monitoring of medium metabolite consumption and production. This study reports on growth of CCO cell line during 9 days of cultivation in two different media, DMEM (ATCC) + 10% FBS and D-MEM/F12 (Gibco) + 10% NCS. Growth of CCO cell line was found better in DMEM medium with 10% FBS than in medium with 5% or 2,5% FBS. Consumption of medium metabolites was monitored during 5 days of growth of CCO and CHO-K1 cell lines. The results show that CCO cells do not use glucose as a primary energy source. Possible source of energy for these cells may be amino acids, especially glutamine. However accumulation of lactate as metabolic byproduct was also detected. Metabolite detection of D-MEM/F12 medium showed that CHO-K1 cells use glucose as a main source of energy while lactate accumulates as metabolic byproduct. This indicates of incomplete break-down of glucose. Accumulation of alanine and glycine, as well as consumption of treonine, serine, methionine, arginine, taurine and glutamine in medium for CCO cells cultivation was detected by ion-exchange chromatography. The amino acid detection in medium for CHO-K1 cell cultivation showed accumulation of alanine and ornithine, as well as consumption of asparartate, glutamic acid, glutamine, serine and threonine. Accumulation of lactate and alanine in growth medium for CHO-K1 cells is due to significant glucose and amino acids consumption, which represents typical metabolic disfunction in energy requirement of cultured cells

Interactions of Indole Derivatives with β-Cyclodextrin: A Quantitative Structure-Property Relationship Study.

Retention factors for 31 indole derivatives, most of them with auxin activity, were determined by high-performance liquid chromatography, using bonded β-cyclodextrin as a stationary phase. A three-parameter QSPR (quantitative structure-property relationship) model, based on physico-chemical and structural descriptors was derived, which accounted for about 98% variations in the retention factors. The model suggests that the indole nucleus occupies the relatively apolar cavity of β-cyclodextrin while the carboxyl group of the indole -3-carboxylic acids makes hydrogen bonds with the hydroxyl groups of β-cyclodextrin. The length and flexibility of the side chain containing carboxyl group strongly affect the binding of these compounds to β-cyclodextrin. Non-acidic derivatives, unlike the indole-3-carboxylic acids, are poorly retained on the column. A reasonably well correlation was found between the retention factors of the indole-3-acetic acids and their relative binding affinities for human serum albumin, a carrier protein in the blood plasma. A less satisfactory correlation was obtained when the retention factors of the indole derivatives were compared with their affinities for auxin-binding protein 1, a plant auxin receptor

Scatter plot of the relationship between relative binding affinities of indole derivatives for human serum albumin (log <i>k</i>_HSA) and their retention factors obtained on Astec Cyclobond^™ I 2000 column (log <i>k</i>_CD).

<p>The equation for the regression line is: log <i>k</i>_HSA = -0.82 (±0.40) + 1.80 (±0.53) log <i>k</i>_CD; <i>n</i> = 18, <i>r</i>² = 0.76, <i>s</i> = 0.16, <i>F</i>^1,16 = 52.2.</p

Williams plot of standardized residuals (<i>y</i>-axis) versus hat values (<i>x</i>-axis) for the final model Eq (9).

<p>The horizontal and vertical lines represent ± 3 standardized residuals and warning hat value (h* = 0.48), respectively. The compounds 3 and 5 are slightly influential compounds in the training set, with hat values (h = 0.50) somewhat higher than the warning hat value.</p

Octanol-water partition coefficient and indicator variables (meaning given in Results and discussion) plus observed and calculated retention factors of the studied indole derivatives obtained on Astec CyclobondTM I 2000 column.

<p>Octanol-water partition coefficient and indicator variables (meaning given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0154339#sec009" target="_blank">Results and discussion</a>) plus observed and calculated retention factors of the studied indole derivatives obtained on Astec CyclobondTM I 2000 column.</p

General structure of indole derivatives.

<p>General structure of indole derivatives.</p

Sorption of divalent heavy metal ions onto functionalized biogenic hydroxyapatite with caffeic acid and 3,4-dihydroxybenzoic acid

The sorption ability of biogenic hydroxyapatite (BHAP) towards heavy metal ions (Pb, Cu, Ni, Cd, and Zn) is compared with functionalized BHAP powders with caffeic acid (CA) and 3,4-dihydroxybenzoic acid (3,4-DHBA). The functionalization of the BHAP with either CA or 3,4-DHBA is indicated by the appearance of the colored powders due to the formation of the interfacial charge transfer (ICT) complexes. The detailed characterization of as-prepared and functionalized BHAP samples was performed using transmission electron microscopy, reflection spectroscopy, thermogravimetric analysis and determination of zeta potential. All three sorbents clearly displayed preferential sorption of Pb ions when the total concentration of multi-component equimolar solutions of heavy metal ions is high. It should be emphasized that the sorption capacity of functionalized BHAP with either CA or 3,4-BHAP was found to be higher, up to 60%, compared to as-prepared BHAP without the decrease of selectivity towards Pb ions. © 2019, © 2019 Taylor & Francis Group, LLC.Supporting information: Sorption of divalent heavy metal ions onto functionalized biogenic hydroxyapatite with caffeic acid and 3,4-dihydroxybenzoic acid. Taylor & Francis. Smičiklas, Ivana D.; Lazić, Vesna M.; Živković, Ljiljana S.; Porobić, Slavica J.; Ahrenkiel, S. Phillip; Nedeljković, Jovan [https://doi.org/10.6084/m9.figshare.8068580.v1]Accepted manuscript version (postprint) available at: [http://vinar.vin.bg.ac.rs/handle/123456789/8188

Ispitivanje antimikrobnih svojstava nekih biđineli-azo piridonskih boja

The heterocyclic azo dyes represent significant synthetic organic colorants. In addition to their exceptional coloration properties, heterocyclic azo compounds exhibit antibacterial, antifungal, an-tiviral, antioxidant, anti-inflammatory and anticancer properties. The antimicrobial resistance is one of the major problems for global public health. Therefore, finding new organic structures with anti-microbial properties represents a challenging research field. In this study, antimicrobial screening of two Biginelli-based azo pyridone dyes has been carried out. The antimicrobial activity of synthe-tized dyes wasstudied against bacteria (Staphylococcus aureus, Escherichia coli) and yeast (Candida albicans), using agar diffusion method. The preliminary research results indicate the antimicrobial potential of the investigated compounds.Heterociklična azo jedinjenja predstavljaju značajnu klasnu organskih sintetskih boja. Pored njihovih izuzetnih svojstava za bojenje, heterociklična azo jedinjenja imaju i biološku aktivnost. Mnoge studije pokazuju da azo boje sa heterocikličnim fragmentima u strukturi mogu ispoljavati antibakterijska, antifungalna, antivirusna, antiinflamatorna i antikancerogena svojstva. Rezistencija mikroorganizama na postojeće antimikrobne agense predstavlja jedan od najvećih problema u svetskom zdravstvu. Zbog toga je razvijanje novih molekula sa antimikrobnim svojstvima kako značajno, tako i neophodno za globalno očuvanje zdravlja. U ovom radu, ispitivanje antimikrobne aktivnosti dve Biđineli-azo piridonske boje je urađeno pomoću metode difuzije na agarnoj podlozi. Antimikrobna svojstva su ispitana prema patogenim sojevima Staphylococcus aureus (Gram-pozitivna bakterija), Escherichia coli (Gram-negativna bakterija) i Candida albicans (oportunistički kvasac). Preliminarni rezultati istraživanja ukazali su na antimikrobni potencijal ispitivanih azo jedinjenja