30 research outputs found
Genetic polymorphism of glutathion S-transferase P1 (GSTP1) Ile105Val and susceptibility to atherogenesis in patients with type 2 diabetes mellitus
One of the characteristics of type 2 diabetes mellitus (T2DM) is the state of persistent oxidative stress (OS) that has been implicated in the pathogenesis of diseases such is atherosclerosis mainly through chronic hyperglycemia that stimulates production of reactive oxygen species (ROS) and increases OS. Glutathione S-transferase P1 (GSTP1) is a member of the cytosolic GST superfamily. It plays an important role in neutralizing OS as an enzyme. Also, it participates in regulation of stress signaling and protects cells against apoptosis via its noncatalytic ligand-binding activity. GSTP1 Ile105Val functional polymorphism influences protein catalytic activity and stability and the aim of this study was to determine whether this gene variation influences susceptibility to atherogenesis in T2DM patients. A total of 240 individuals (140 patients with T2DM, accompanied with clinical manifestations of atherosclerosis, and 100 healthy controls) were included in this study. Genomic DNA was isolated from peripheral blood cells and genotyping was performed using polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) analysis. We obtained no statistically significant differences in the distribution of alleles and genotypes between cases and controls (P>0.05) but association between Ile/Val (OR=0.6, 95%CI=0.35-1.05, P=0.08) and Val/Val (OR=0.45, 95%CI=0.18-1.11, P=0.08) genotypes and disease approached significance (P=0.08). Our results indicated that a larger study group is needed to establish the true relationship between potentialiy protective allele Val and the disease, and to determine the influence of other GSTP1 polymorphisms on atherogenesis in T2DM patients. [Projekat Ministarstva nauke Republike Srbije, br. 175075
GenetiÄki polimorfizam glutation S-transferaze P1 (GSTP1) Ile105Val i osetljivost na aterogenezu kod pacijenata sa dijabetesom tipa 2
One of the characteristics of type 2 diabetes mellitus (T2DM) is the state of persistent oxidative stress (OS) that has been implicated in the pathogenesis of diseases such is atherosclerosis mainly through chronic hyperglycemia that stimulates production of reactive oxygen species (ROS) and increases OS. Glutathione S-transferase P1 (GSTP1) is a member of the cytosolic GST superfamily. It plays an important role in neutralizing OS as an enzyme. Also, it participates in regulation of stress signaling and protects cells against apoptosis via its noncatalytic ligand-binding activity. GSTP1 Ile105Val functional polymorphism influences protein catalytic activity and stability and the aim of this study was to determine whether this gene variation influences susceptibility to atherogenesis in T2DM patients. A total of 240 individuals (140 patients with T2DM, accompanied with clinical manifestations of atherosclerosis, and 100 healthy controls) were included in this study. Genomic DNA was isolated from peripheral blood cells and genotyping was performed using polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) analysis. We obtained no statistically significant differences in the distribution of alleles and genotypes between cases and controls (P>0.05) but association between Ile/Val (OR=0.6, 95%CI=0.35-1.05, P=0.08) and Val/Val (OR=0.45, 95%CI=0.18-1.11, P=0.08) genotypes and disease approached significance (P=0.08). Our results indicated that a larger study group is needed to establish the true relationship between potentialiy protective allele Val and the disease, and to determine the influence of other GSTP1 polymorphisms on atherogenesis in T2DM patients.Jedna od osobina tipa 2 dijabetes mellitus (T2DM) je stanje perzistentnog oksidativnog stresa (OS) koji je sastavni deo patogeneze bolesti kao Å”to je ateroskleroza i to najÄeÅ”Äe putem hroniÄne hiperglikemije koja stimuliÅ”e nastanak reaktivnih vrsta kiseonika (tzv ROS) i poveÄava nivo OS. Glutation S-transferaza P1 (GSTP1) pripada superfamiliji citosolnih GST. Kao enzim, ima važnu ulogu u neutralizaciji OS. TakoÄe, uÄestvuje u regulaciji signalnog puta stresa ali i Å”titi Äelije od apoptoze putem nekatalitiÄke ligand-vezujuÄe aktivnosti. Funkcionalni polimorfizam Ile105Val GSTP1 gena utiÄe na katalitiÄku aktivnost proteina i na njegovu stabilnost i cilj ove studije je bio da se utvrdi da li ova genska varijanta utiÄe na osetljivost na aterogenezu kod pacijenata sa T2DM. Ukupno 240 osobe (140 pacijenata sa T2DM i nekom od kliniÄkih manifestacija ateroskleroze i 100 zdravih kontrola) je bilo ukljuÄeno u ovu studiju. Genomska DNK je izolovana iz Äelija periferne krvi a genotipizacija je uraÄena primenom reakcije lanÄane polimeraze posle koje je uraÄena analiza dužine restrikcionih fragmenata (PCR-RFLP analiza). Nismo dobili statistiÄki znaÄajne razlike u raspodeli alela i genotipova izmeÄu obolelih i kontrola (P>0.05) ali asocijacija izmeÄu Ile/Val (OR=0.6, 95%CI=0.35-1.05, P=0.08) i Val/Val (OR=0.45, 95%CI=0.18-1.11, P=0.08) genotipova i bolesti se približila znaÄajnosti (P=0.08). NaÅ”i rezultaati pokazuju da je potrebna veÄa grupa ispitanika da bi se utvrdila prava veza izmeÄu potencijalno protektivnog alela Val i bolesti kao i da bi se utvrdio uticaj drugih polimorfizama gena GSTP1 na aterogenezu kod T2DM pacijenata
Multiple Roles of LOXL2 in the Progression of Hepatocellular Carcinoma and Its Potential for Therapeutic Targeting
LOXL2, a copper-dependent amine oxidase, has emerged as a promising therapeutic
target in hepatocellular carcinoma (HCC). Increased LOXL2 expression in HCC has been linked
with an aggressive phenotype and represents a poor prognostic factor. Here, we focus on the
mechanisms through which LOXL2 orchestrates multiple oncogenic functions in HCC development.
We performed a review of the current knowledge on the roles LOXL2 performs in the modulation of
the HCC tumor microenvironment, formation of premetastatic niches, and epithelialāmesenchymal
transition. We also highlighted the complex interplay between LOXL2 and hypoxia, angiogenesis,
and vasculogenic mimicry in HCC. At the end of the review, we summarize the current LOXL2
inhibitors and discuss their potential in HCC precision treatment
Antioxidant Response in Gills of Eurasian Perch ( Perca fluviatilis ) to Cyanobacterial Bloom Exposure in the Gruža Reservoir
The aim of this study was to assess the impact of an Aphanizomenon flos-aquae bloom in the Gruža Reservoir on the antioxidant parameters measured in the gill cells of Eurasian perch (Perca fluviatilis). Effects of the bloom were evaluated through copper, zinc and manganese containing superoxide dismutase (CuZnSOD and MnSOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and biotransformation phase II enzyme glutathione-S-transferase (GST) activities and glutathione (GSH) and sulfydryl (āSH) groups concentrations. The activities of CuZn SOD and Mn SOD decreased during the cyanobacterial bloom compared to the period before the bloom. The opposite trend was observed for CAT and GSH-Px activities that increased. During the bloom GR and GST activities and GSH concentration decreased significantly. CuZn SOD, GR and GST activities are detected as the most important parameters for individual variations. Our results indicated that an A. flos-aquae bloom promotes oxidative stress in the gills of P. fluviatilis. The investigated parameters could be used in environmental risk assessment procedures to monitor the effects of A. flos-aquae blooms on fish. Observed changes in the antioxidant parameters in P. fluviatilis gills make them potential biomarkers in the research of ecological risk situations in freshwater ecosystems with frequent cyanobacterial blooms.Water Research and Management (2016), 6(2): 19-2
Chokeberry (Aronia melanocarpa) fruit extract modulates immune response in vivo and in vitro
Chokeberry (Aronia melanocarpa) is known for its anti-oxidant, anti-inflammatory and anti-diabetic properties. Since the effects of chokeberry extract on the immune response have been only sporadically assessed, our aim was to investigate chokeberry fruit water extract on the immune response in vivo and in vitro. When administered orally to healthy mice, the extract exerted immunomodulatory effects in the gut evidenced by the altered proportion of macrophages, dendritic cells and T cells. Importantly, oral consumption of the chokeberry extract resulted in blood glucose level increase in C57BL/6 mice with chemically-induced diabetes. These in vivo results were corroborated by observed up-regulation of nitric oxide and interelukin-1Ī² production in macrophages and dendritic cells, up-regulated phagocytic activity of macrophages, increased T and B lymphocytes proportions and differentiation of interferon-Ī³-producing T cells in vitro. The obtained results imply that our chokeberry extract stimulates pro-inflammatory properties in immune cells of innate and adaptive immunity
The influence of specific aspects of occupational stress on security guards' health and work ability: detailed extension of a previous study
In our earlier study of security guards, we showed that higher occupational stress was associated with health impairments (metabolic syndrome, diabetes, hypertension, cardiovascular diseases) and work disability. The aim of this study was to further explore the association of specific occupational stressors with health impairments and work disability parameters in 399 Serbian male security guards (aged 25-65 years). Ridge linear regression analysis revealed that, after controlling for age, body mass index, and smoking status, professional stressors including high demands, strictness, conflict/uncertainty, threat avoidance and underload were significant positive predictors of fasting glucose, triglycerides, total and LDL cholesterol, blood pressure, heart rate, Framingham cardiovascular risk score, and temporary work disability. The security profession is in expansion worldwide, and more studies are needed to establish precise health risk predictors, since such data are generally lacking
GLYCOSIDE HYDROLASES FROM FRESHWATER FISH GILL MICROBIOTA AS BIOFILM INHIBITORS FOR ENHANCED FOOD SAFETY
The formation of biofilms by foodborne pathogens
is a constant challenge in the food industry,
leading to an increased risk of contamination and
compromising food safety. Many of the chemicals
commonly used for sanitation in the food industry
are unable to remove biofilms, are harmful
to surfaces and can be toxic. The effectiveness
of disinfectants can be improved using enzymes
that specifically target biofilm components such
as exopolysaccharides, extracellular DNA, or proteins.
In this study we investigated the potential
of glycoside hydrolases originating from the
gill microbiota of freshwater fish to control biofilm
formation in the most common foodborne
pathogens. We demonstrated that Ī²-glucosidase
from Microbacterium sp. BG28 (BglB-BG28) effectively
inhibits cellulose-rich biofilms formed by
Salmonella enteritidis, S. typhimurium, S. infantis,
and Escherichia coli. When these bacteria were cultivated overnight with 200 Ī¼L/mL enzyme, up
to 80% less biofilm was formed. By fluorescence
microscopy, we visualised the inhibition of biofilms
on plastic, glass and aluminium, materials
commonly used in the food industry. When used
as a pre-treatment, BglB-BG28 increased the
bactericidal efficacy of OxicidĀ®S, a commercially
available surface disinfectant. Its effectiveness at
temperatures up to 50 Ā°C and in a pH range from
4 to 8 together with compatibility with non-ionic
detergents and high tolerance to sodium chloride
and glucose give BglB-BG28 advantages in
harsh and diverse industrial environments. Importantly,
no toxicity to Caenorhabditis elegans
was observed at enzyme concentrations of up
to 1 mg/ml. Overall, these results demonstrate
the suitability of the Ī²-glucosidase BglB-BG28 for
the formulation of a novel enzyme-based disinfectant
to be used in food processing facilities.Book of abstract: From biotechnology to human and planetary health XIII congress of microbiologists of Serbia with international participation Mikromed regio 5, ums series 24: 4th ā 6th april 2024, Mona Plaza hotel, Belgrade, Serbi
The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier.
Macrophage migration inhibitory factor (MIF) is a multifunctional protein that is involved in the development of gut-related inflammation. To investigate the role of MIF in the function of the intestinal barrier, we have explored intestinal permeability and gut-associated immune response in MIF-deficient (MIF-KO) mice. The absence of MIF provoked impairment of tight and adherens epithelial junctions in the colon through the disturbance of E-cadherin, zonula occludens-1, occludin and claudin-2 expression, which lead to the increase of intestinal barrier permeability. In these circumstances the diversity and content of gut microbiota in MIF-KO mice was considerably different compared to wild type mice. This change in microbiota was accompanied by an increased intestinal IgA concentration and a higher production of pro-inflammatory cytokines TNF and IFN-Ī³ in mesenteric lymph nodes of MIF-KO mice. The forced changes of microbiota executed by antibiotics prevented the "leakage" of the barrier in MIF-KO mice, probably through up-regulation of occludin expression and normalization of cellular pore diameters. In addition, cytokine secretion was normalized after the treatment with antibiotics. These results suggest that MIF participates in the maintenance of physiological microbiota diversity and immunosurveillance, which in turn enables the proper intestinal barrier function
Health-related quality of life in elderly patients hospitalized with chronic heart failure
Background: Chronic heart failure is a very common condition in the elderly, characterized not only by high mortality rates, but also by a strong impact on health-related quality of life (HRQOL). Previous studies of HRQOL in elderly heart failure subjects have included mostly outpatients, and little is known about determinants of HRQOL in hospitalized elderly population, especially in Serbia. In this study, we tried to identify factors that influence HRQOL in elderly patients hospitalized with chronic heart failure in Serbia. Methods: The study population consisted of 136 patients aged 65 years or older hospitalized for chronic heart failure. HRQOL was assessed using the Minnesota Living with Heart Failure questionnaire. Predictors of HRQOL were identified by multiple linear regression analysis. Results: Univariate analysis showed that patients with lower income, a longer history of chronic heart failure, and longer length of hospital stay, as well as those receiving aldosterone antagonists and digoxin, taking multiple medications, in a higher NYHA class, and showing signs of depression and cognitive impairment had significantly worse HRQOL. Presence of depressive symptoms (P lt 0.001), higher NYHA class (P=0.021), lower income (P=0.029), and longer duration of heart failure (P=0.049) were independent predictors of poor HRQOL. Conclusion: Depressive symptoms, higher NYHA class, lower income, and longer duration of chronic heart failure are independent predictors of poor HRQOL in elderly patients hospitalized with chronic heart failure in Serbia. Further, there is an association between multiple medication usage and poor HRQOL, as well as a negative impact of cognitive impairment on HRQOL. Hence, measures should be implemented to identify such patients, especially those with depressive symptoms, and appropriate interventions undertaken in order to improve their HRQOL
Ultimate pH, colour characteristics and proximate and mineral composition of edible organs, glands and kidney fat from Saanen goat male kids
Ultimate pH value and instrumental colour (CIEL*a*b* values) characteristics, proximate (moisture, protein, total fat and total ash) and mineral composition (K, P, Na, Mg, Ca, Zn, Fe, Cu, Ni and Mn) were determined in 10 (heart, tongue, lungs, spleen, liver, kidney, brain, testicle, thymus and kidney fat) edible by-products of Saanen goat male kids. Many significant or numerical differences were found in the mean values of quality characteristics among the edible by-products. Among edible organs and glands, liver had the lowest surface CIEL* value (darkest colour), and the highest levels of protein, Zn, Cu and Mn. Furthermore, the highest pH(24h), total ash, K, P and Mg levels were determined in the thymus. The testicle had the highest moisture, Ca and Ni levels. The spleen had the lowest fresh cut cross-section CIEL* value (darkest colour), and the highest Fe level. The highest total fat content and Na level were determined in the brain and kidney, respectively. Among all the edible by-products, kidney fat had the highest pH(24h), surface CIEL* value (lightest colour) and total fat content, and the lowest moisture, protein, total ash, K, P, Na, Mg, Ca, Zn, Fe, Cu, Ni and Mn levels