Komparativno ispitivanje lokalnog terapijskog dejstva probiotika, aktivnog kiseonika i klindamicin gela kod pacijenata sa hroničnom parodontopatijom

The aim of the study, which was conducted as a clinical prospective study, was to determine the effectiveness of basic periodontal therapy, local application of probiotics, active oxygen and clindamycin gel after basic periodontal disease therapy in subjects with chronic periodontitis. Different approaches to the treatment of chronic periodontitis have been compared. In addition to basic (causal) therapy, which is the "gold standard" in the treatment of chronic periodontitis, probiotic, active oxygen and clindamycin gel therapy was used. All subjects underwent a detailed clinical examination and accurate anamnestic data were taken. The dental examination revealed a chronic form of periodontitis in all subjects, which was confirmed by radiological examination. Clinical parameters were determined before therapy, on the seventh day and 30 days after therapy. Subgingival specimens were collected before basic therapy and after 30 days to identify the following bacterial species: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis and Treponema denticola. The results were compared by clinical examination (oral hygiene indices, gingival and periodontal index) and polymerase chain reaction (PCR) by the method for testing periodontopathogenic bacteria. The study involved 160 respondents divided into 4 groups. The first group consisted of 40 subjects with chronic periodontitis who received only basic therapy (control group). The second group consisted of 40 subjects with chronic periodontitis who were treated with probiotic bacteria, Probiotic® (Ivančić and sons, Hemofarm Stada group, Vršac) in the periodontal pocket for 5 days. The third group consisted of 40 subjects with chronic periodontitis who, after basic therapy, received active oxygen therapy in liquid form (Deverra, Farm DOO, Lebane) in the periodontal pocket for 5 days. The fourth group of subjects, a total of 40, with a chronic form of periodontitis, received basic periodontal therapy, after which clindamycin gel (clindamycin hydrochloride gel 2%) was applied topically to the periodontal pocket, once. The clinical trial showed better results in groups II, III and IV compared to the first control group of subjects in whom only basic periodontal therapy was used. After therapy, a reduction in the presence of the following bacterial species was found: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis and Treponema denticola (p <0.001). Based on this clinical trial, it can be concluded that the combined basic therapy and local application of probiotics and active oxygen gave better results compared to the control group in which only basic therapy was used

Newly designed hemagglutinin-Der p 2 chimera is a potential candidate for allergen specific immunotherapy

Aim To investigate the immunomodulatory potential of a chimera composed of the receptor-binding domain of hemagglutinin 1 (H1s) from Influenza virus and Der p 2 (D2) allergen for allergen-specific immunotherapy of house-dust mite allergy (HDM). Main methods: H1sD2 chimera and D2 allergen were produced by genetic engineering in E. coli. Recombinant antigens were extracted from inclusion bodies by urea, then refolded and purified by immobilized-metal affinity chromatography (IMAC). Purity was verified by 2D-PAGE and secondary structures were assessed by CD spectroscopy. IgE reactivity of H1sD2 and D2 was tested in western blot with sera from 8 persons with clinical history of HDM allergy. Immunogenicity of H1sD2 and D2 were analyzed in Balb/c mice. Cytokine profile was analyzed by ELISA after stimulation of mouse spleen cells with H1sD2 and D2. Leukocyte population abundance of cells isolated from spleen and lymph node was assessed by flow cytometry. Key findings: Purified recombinant proteins H1sD2 (42 kDa) and D2 (15 kDa) revealed well defined secondary structures, and preserved IgE reactive epitopes. Analysis of supernatants of mouse spleen cells after stimulation with H1sD2 and D2, revealed a qualitatively different cytokine profile from H1sD2 immunized mouse cells (increase in IL10). CD8+ cells were decreased in the lymph node of D2 immunized mice, whereas H1sD2 immunization led to an increase of CD8+ cells in both the lymph node and the spleen. Significance: H1sD2 chimera attenuates Der p 2-inherent Th2 response and directs the immune response toward Th1 and Treg phenotype

Terapijska učinkovitost klindamicin gela kao dodatak bazičnoj terapiji kronične parodontopatije

Clindamycin, a lincosamide antibiotic, has been under-recognized as an antimicrobial agent for use in dentistry. The aim of the present work was to evaluate clinical efficacy of 2% clindamycin gel in addition to the basic mechanical periodontal therapy. At baseline, scaling and root planing (SRP) was performed at all 50 subjects (control group and test group). Clindamycin gel was applied after SRP only in the test group. Clinical measurements including periodontal pocket depth (PPD), clinical attachment level (CAL), bleeding on probing (BOP) and plaque index (PI) were done at baseline, and at 3 and 6 months after treatment. Compared to baseline, the PPD and CAL values significantly decreased in the test group (p<0.05) and were statistically lower (p<0.05) compared to control group. PPD reduction of 2.42 mm was obtained in the test group and could be generally considered as clinically significant. A PPD reduction greater than 2 mm indicated that clindamycin gel could be used efficiently as an adjunct to SRP. Also, between-group difference in BOP and PI scores was statistically significant 6 months after treatment. In conclusion, the application of clindamycin gel in combination with SRP enhanced the efficacy of non surgical periodontal therapy in reducing pocket depth and improving attachment levels in chronic periodontitis subjects and had additional benefits over mechanical therapy alone.Klindamicin, linkozamidni antibiotik, je u širokoj upotrebi u stomatologiji. Cilj ovoga rada je bio procijeniti kliničku učinkovitost 2% klindamicin gela kao dodatne terapije bazičnoj terapiji kronične parodontopatije. Bazična terapija provedena je u svih 50 pacijenata (kontrolna skupina i ispitna skupina). Klindamicin gel je primijenjen nakon bazične terapije parodontopatije samo u ispitnoj skupini. Praćeni su sljedeći klinički parametri: dubina parodontalnog džepa (DPDZ), razina pripojnog epitela (NPE), indeks krvarenja (Ikr), plak indeks (PI). Mjerenje indeksa provedeno je prilikom prve terapije te tri i šest mjeseci nakon tretmana. DPDZ i NPE vrijednosti su bile značajno smanjene u ispitnoj skupini (p<0,05) i bile su statističke niže u odnosu na kontrolnu skupinu (p<0,05). Smanjenje dubine parodontalnog džepa za 2,42 mm zabilježeno je u ispitnoj skupini i može se smatrati klinički značajnim. Ovo smanjenje pokazuje da klindamicin gel ima terapijski učinak kao dodatak bazičnoj terapiji u liječenju kronične parodontopatije. Također je evidentirana statistička razlika između skupina u Ikr i PI šest mjeseci nakon terapije. Zaključuje se da primjena klindamicin gela u kombinaciji s bazičnom terapijom parodontopatije povećava učinkovitost nekirurške terapije

Verminozni gastriti svinja

Verminal gastritis are swine parasitoses caused by nematodes of genus Spiruridea (Arduena strongyllinae, Gnathostoma hispidum,Physocephalus sexalatus and Simondsia paradoxa) and Trichostrongylidae (Hyostrongylus rubidus and Ollulanus tricuspis). In Serbia H. rubidus were occurred at 3-6% farm breeding swine and infection with Arduena strongyllinae, Gnathostoma hispidum, Physocephalus sexalatus and Hyostrongylus rubidus were occurredat extensive breeding condition. Pathology changes were at deep part of stomach mucus where we found deep wound and bacterial infection at fixation places. Clinical signs are apathy, slenderness, convulsion and at hard infection possibility died.Verminozni gastriti svinja je obolenje farmskih i ekstenzivno držanih svinja uzrokovano sa nematodama iz rodova Spiruridea i Trichostrongylidae. Obolenja su raširena širom sveta. U našoj zemlji, H.rubidus se sreće kod 3-6% farmski držanih svinja, dok je u ekstenzivnom držanju ustanovljena infekcija sa Ascarops strongyllinae, Gnathostoma hispidum, Physocephalus sexalatus i Hyostrongylus rubidus ali prevalence nije poznata. Patološko delovanje se ispoljava u vidu dubokih rana u sluznici želuca i posledičnim infekcijama na mestu fiksacije. Od kliničkih simptoma prisutni su apatija, mršavljenje, anemija, a kod jakih infekcija i uginuća. Značaj koji verminozni gastriti imaju kod svinja daleko prevazilazi pažnju koja joj se poklanja u našoj sredini i morala bi biti predmet jednog studioznog istraživanja

Predictors of Vancomycin-Resistant Enterococcus spp. Intestinal Carriage among High-Risk Patients in University Hospitals in Serbia

The predictors of intestinal carriage of vancomycin-resistant Enterococcus spp. (VRE) among high-risk patients in the counties of the Southeast Europe Region are insufficiently investigated, yet they could be of key importance in infection control. The aim of the study was to identify risk factors associated with fecal VRE colonization among high-risk inpatients in university hospitals in Serbia. The study comprised 268 inpatients from three university hospitals. Data on patient demographics and clinical characteristics, length of hospital stay, therapy, and procedures were obtained from medical records. Chi-squared tests and univariate and multivariate logistic regressions were performed. Compared to the hemodialysis departments, stay in the geriatric departments, ICUs, and haemato-oncology departments increased the risk for VRE colonization 7.6, 5.4, and 5.5 times, respectively. Compared to inpatients who were hospitalized 48 h before stool sampling for VRE isolation, inpatients hospitalized 3–7, 8–15, and longer than 16 days before sampling had 5.0-, 4.7-, and 6.6-fold higher risk for VRE colonization, respectively. The use of cephalosporins and fluoroquinolones increased the risk for VRE colonization by 2.2 and 1.9 times, respectively. The age ≥ 65 years increased the risk for VRE colonization 2.3 times. In comparison to the University Clinical Centre of Serbia, the hospital stays at Zemun and Zvezdara University Medical Centres were identified as a protector factors. The obtained results could be valuable in predicting the fecal VRE colonization status at patient admission and consequent implementation of infection control measures targeting at-risk inpatients where VRE screening is not routinely performed

Askaridoza - stalno prisutan problem intenzivne svinjarske proizvodnje

Ascaridosis presence mast frequent, and most important, with global distribution with prevalence, dependent of bruding condition, were 5-95%. Chronical development of diseases induce permanent lasses at swine production through by low growth, weakly product results and by condemned of organs when presence larvar migration. At our parers we give outline on most important moment connected with swine askaridosis spread of parasites in our countries, pathogenesis and pathology changes and measure to prevention and eradication of diseases.Askaridoza predstavlja najčešću i najrasprostranjeniju parazitsku infekciju svinja, globalne distribucije, čija se prevalenca zavisno od uslova držanja kreće 5- 95%. Protičući u hroničnom obliku uzrokuje gubitke u svinjarskoj proizvodnji koji se manifestuju u vidu umanjenog prirasta, slabije konverzije hrane i odbacivanja organa kroz koje se odvija migratorna faza parazita. U našem radu dajemo kratak osvrt na najvažnije momente vezane za askaridozu svinja - raširenost ove parazitoze kod nas, patogenezu i patološke promene kao i mere preventive i suzbijanja oboljenja

Šuga - stalno prisutan problem intenzivne svinjarske proizvodnje

Scabies caused by Sarcoptes scabei var. suis presence most frequent, and most important health problem in farm breeding swine with global distribution with prevalence, dependent of bruding condition, were 5-95%. Chronical development of diseases induce permanent lasses at swine production through by low growth, weakly product results and by condemned of organs when presence larvar migration. At our papers we give outline on most important moment connected with swine scabies spread of parasites in our countries pathogenesis and pathology changes and measure to prevention and eradication of diseases.Šuga uzrokovana sa Sarcoptes scabei var. suis predstavlja jednu od najčešćih i najrasprostranjenijih parazitsku infekciju svinja, globalne distribucije čija se prevalenca zavisno od uslova držanja kreće 5-95%. Protičući u akutnom a potom hroničnom obliku uzrokuje rapidne gubitke svinjarskoj proizvodnji kroz umanjen prirast i slabije proizvodne rezultate. U našem radu dajemo kratak osvrt na najvažnije momente vezane za šugu svinja raširenost ove parazitoze kod nas, patogenezu i patološke promene kao i mere preventive i suzbijanja obolenja

Oxidation-reduction potential in the milk from mothers of preterm infants

Health benefits of mother's milk depend on the level of the total antioxidant capacity (TAC). In mother's milk, the TAC should refer to the sum of activities derived from active enzymatic antioxidant systems (e.g. superoxide dismutase, catalase, glutathione peroxidase etc.), non-enzymatic antioxidants, such as vitamins C and E, and the presence of other bioactive factors (e.g. lactoferrin, uric acid etc.). Measuring oxidation-reduction potential (ORP) using RedoxSYS Analyzer may be an alternative to classic methods of measuring TAC. We compared the results of ORR and ascorbic acid content in milk from mothers of preterm infants.Abstract: [https://cer.ihtm.bg.ac.rs/handle/123456789/5531]Poster presented at 3rd International Conference on Nutrition & Growth, March 17-19, 2016, Vienna, Austri

Oxidation-reduction potential in the milk from mothers of preterm infants

Health benefits of mother's milk depend on the level of the total antioxidant capacity (TAC). In mother's milk, the TAC should refer to the sum of activities derived from active enzymatic antioxidant systems (e.g. superoxide dismutase, catalase, glutathione peroxidase etc.), non-enzymatic antioxidants, such as vitamins C and E, and the presence of other bioactive factors (e.g. lactoferrin, uric acid etc.). Measuring oxidation-reduction potential (ORP) using RedoxSYS Analyzer may be an alternative to classic methods of measuring TAC. We compared the results of ORR and ascorbic acid content in milk from mothers of preterm infants.Poster: [https://cer.ihtm.bg.ac.rs/handle/123456789/5532

Periodontal medicine: The emergence of a new branch in periodontology

Introduction: Periodontal medicine is the emergence of a new branch of periodontology. Periodontal medicine is a term used for different purposes in different parts of the world. In certain countries, it relates to the study of the dynamic relationship between periodontal diseases and systemic conditions, such as cardiovascular and cerebrovascular disease, preterm delivery low-birth-weight babies, diabetes mellitus, osteoporosis, and disorders of the respiratory tract. Such studies investigate the peripheral impacts of periodontal inflammation on systemic health. In other countries, 'periodontal medicine' is a term used to describe the periodontal (and gingival) manifestations of medical conditions. This includes their investigation, diagnosis and therapeutic management and how management of the oral condition integrates with the patient's medical management as part of a holistic approach within defined care pathways. Conclusion: The text is a comprehensive analysis of research on periodontal disease and its link to systemic conditions. The periodontal treatment may be important in terms of preventing a patient's overall health. Periodontologists emphasize that infections in the mouth can cause health problems in other parts of the body