16 research outputs found
LACTATE DEHYDROGENASE GENE GENOTYPIZATION FOR SPECIES IDENTIFICATION IN A FISH FARM ON THE RIVER NERETVA
There are severale Salmonid species, found in the river Neretva basin, among which S. trutta and S. obtusirostris. Also, natural hybrids such as S. obtusirostris x S. trutta have been observed. In one fish farm on the river Neretva, S. trutta and S. obtusirostris were decided to breed separately. Parental fishes were separated phenotypicaly on the basis of the morphological signs. PCR-RFLP analysis of the exon 3 to exon 4 part of the lactate dehydrogenase (LDH) C1* gene with restriction endonuclease RsaI was employed to identify the presence of other species representatives or intercrosses in two groups of juvenille fishes. Using this method, we were able to identify two S. trutta representatives in the S. obtusirostris group
Pathohistological approach to liver transplantation
Jetra je vitalni organ Äovjeka. Ima važnu ulogu u metabolizmu hranjivih tvari i lijekova, sintezi proteina i filtraciji krvi. Zbog svoje složene graÄe i mnogobrojnih funkcija jetra je Äesto sijelo bolesti. Postoje mnogobrojna patoloÅ”ka stanja jetre, no u kontekstu transplantacijske patologije najbitnija su ona koja dovode do akutnog zatajenja jetre, kroniÄnog zatajenja jetre i ciroze. Ove bolesti lijeÄe se transplantacijom jetre. Transplantacijska patologija bavi se bolestima koje se pojavljuju na alotransplantatu: prezervacijsko oÅ”teÄenje, vaskularne komplikacije, komplikacije žuÄnih vodova, odbacivanje organa, infekcije i recidivirajuÄe bolesti. Prezervacijsko oÅ”teÄenje nastaje kao posljedica produljenog vremena tople i hladne ishemije. Vaskularne komplikacije i komplikacije žuÄnih vodova najÄeÅ”Äe su posljedica kirurÅ”kih komplikacija. Infekcije hepatitis B virusom i hepatitis C virusom Äesto recidiviraju i progresivno vode do zatajenja alotransplantata. Odbacivanje organa dijeli se na humoralno, akutno i kroniÄno. RecidivirajuÄe bolesti ukljuÄuju infektivne, imunoloÅ”ke, tumorske i toksiÄne bolesti jetre. Glavna dijagnostiÄka metoda veÄine ovih stanja je biopsija jetre i patohistoloÅ”ki pregled tkiva. MeÄutim mikroskopska slika ovih bolesti Äesto je nespecifiÄna i vrlo isprepletena. Neke bolesti posebno je teÅ”ko razlikovati samo na temelju mikroskopske slike. Zbog toga je potrebno poznavati vrijeme proteklo od transplantacije, kliniÄku sliku bolesti i stanje pacijenta te Å”iroku diferencijalnu dijagnozu koja dolazi u obzir.The liver is a vital human organ. It has an important role in nutrient and drug metabolism, protein synthesis and blood filtration. Because of its complex structure and multiple functions the liver is a common site of diseases. There are lots of possible pathological states of the liver, but in the context of liver transplantation pathology the most important are: acute liver failure, chronic liver failure and cirrhosis. These diseases are treated with liver transplantation. Transplantation pathology covers diseases that occur on the allograft: preservation injury, vascular complications, bile duct complications, rejection, infections and reccurent diseases. Preservation injury occurs due to prolonged cold and hot ischemia. Vascular complications and bile duct complications are usually a result of surgical complications. Hepatitis B and hepatitis C infections commonly recur in the allograft and lead to progressive allograft failure. Liver rejection is divided into humoral, acute and chronic. Recurrent diseases include infective, immune, neoplastic and toxic liver diseases. The main diagnostic method for most of these diseases is liver biopsy and pathohistological tissue examination. However the microscopic findings can be unspecific and overlapping. Some diseases can be difficult to distinguish based on microscopic findings alone. Therefore it is important to know the time after transplantation, the clinical presentation of the disease and the broad differential diagnosis that needs to be considere
Carcass Composition and Meat Quality of Crna Slavonska Pigs from Two Different Housing Conditions
The aim of the study was to compare carcass composition and meat quality of pigs held in two housing systems suitable for production of Crna slavonska pigs. The experiment was conducted on 104 Crna Slavonska pigs (equal number of barrows and gilts) from two housing systems, outdoor (n=56) and indoor (deep bedding, n=48). The pigs in the outdoor group were reared until 18 months of age, and pigs in deep bedding group were raised until 15 months of age. After reaching the final age, the pigs were sacrificed and following carcass and meat quality traits were determined at the slaughter house and in laboratory: backfat and muscle thickness, length of the carcass from os pubis to atlas and from os pubis to 1st rib, ham length and circumference, loin eye area and fat area of longissimus lumborum muscle, pH values, water holding capacity and drip loss, colour reflectance scores (CIE L* a* b*) and instrumental tenderness. The carcasses of the pigs raised outdoors had lower backfat thickness, higher muscle thickness and lower fat area than pigs raised on deep bedding. Their carcasses were longer with longer hams. However, their ham circumference, as well as the loin eye area was lower than in pigs raised on deep bedding indicating higher production of muscle tissue in the latter. Out of investigated meat quality traits, the pigs raised outdoor had lower pH24 values measured in logissimus lumborum muscle and in semimembranosus muscle, higher drip loss and water holding capacity, higher cooking loss and yellowness (CIE b*)
TROTTERāS EXTERIOR CHARACTERISTICS AND THEIR IMPACT ON SPORT RESULTS
TrkaÄi konji su jedna od najvrjednijih kategorija u uzgoju sportskih konja, a vrijede onoliko koliko su znaÄajni njihovi plasmani na klasnim utrkama, koji donose novÄanu zaradu. Brojnost kasaÄkih grla, pravilni treninzi, hranidba, održavanje trka, kvalitetnije trkaÄe staze i struÄnost uzgajivaÄa i trenera mogu podiÄi genetsku kakvoÄu hrvatskog uzgoja kasaÄa. S obzirom da je uzgojni cilj iskljuÄivo usmjeren postizanju veÄe brzine i izdržljivosti u kasu, manje pažnje posveÄeno je vanjÅ”tini. Cilj ovog rada bio je istražiti morfoloÅ”ka svojstva kasaÄa i njihovu povezanost, odnosno utjecaj tjelesnih mjera na rekordno istrÄano vrijeme u utrkama. Istraživanje morfoloÅ”kih svojstava odnosilo se na izmjerenih 15 tjelesnih mjera 11 kobila i 9 pastuha pasmine hrvatski kasaÄ. ProsjeÄna visina grebena Å”tapom istraživanjem obuhvaÄenog uzorka iznosila je 153.63 cm. UtvrÄeno je da su pastusi imali statistiÄki visoko znaÄajno (P<0.01) veÄu visinu grebena i Å”irinu ramena u odnosu na kobile. Pastusi su imali statistiÄki vrlo visoko znaÄajno (P<0.001) duže sapi u odnosu na kobile, a kobile su u odnosu na pastuhe imale statistiÄki znaÄajno (P<0.05) Å”ire sapi. Pastusi su bili vrlo visoko znaÄajno (P<0.001) brži od kobila. Rekordno istrÄano vrijeme bilo je u negativnoj korelaciji s visinom grebena (vrpcom i Å”tapom), obujmom grudi, Å”irinom sapi te s visinama leÄa, sapi i korijena repa. Sve ostale tjelesne izmjere nisu bile znaÄajno, veÄ samo pozitivno povezane s rekordno istrÄanim vremenom u utrci.Racehorses are one of the most valuable categories in sport horse breeding, and their value is worth their rank at class races, which bring financial income. The number of trotters, regular training, nourishment, races and qualitative running tracks as well as the competence of breeders and trainers can raise the genetic quality of the Croatian trotter breeding. The breeding aim is exclusively directed to higher speed achievement and trot endurance, less attention is drawn to the exterior. The aim of this work was to elaborate the morphological characteristics of trotters and the
influence of physical measurements on the record time at trotter races. The morphological characteristics research referred to 15 physical measurements of 11 mares and 9 stallions of the breed Croatian trotter. The average withers height of the research included sample was 153.63 cm. It was established that stallions had statistically significantly higher withers (P<0.01) related to mares. Shoulders width was in stallions statistically significantly higher (P<0.01) related to mares. Stallions had statistically highly significantly longer crupper (P<0.001) related to mares, and mares had statistically significantly (P<0.05) wider crupper. The stallions were highly significantly faster (P<0.001) than mares. The record time at races was in negative correlation with the withers height, the chest girth, back height, crupper and tail root height and with crupper width measured with a tape and a rod. All other physical measures were not significantly, but positively related to record running time
Relationship between recipient and donor factors and kidney transplant outcome
Cilj: Iako je transplantacija bubrega najbolja metoda nadomjeÅ”tanja bubrežne funkcije, joÅ” uvijek postoji potreba za poboljÅ”anjem dugoroÄnih ishoda. Cilj rada bio je utvrditi neovisnu povezanost demografskih Äimbenika primatelja i darivatelja, osnovne bubrežne bolesti, trajanja lijeÄenja dijalizom, tkivne nepodudarnosti i senzibilizacije s ishodima transplantacije u suvremenoj kohorti pacijenata kojima je presaÄen bubreg. Ispitanici i metode: U istraživanje su ukljuÄeni pacijenti kojima je transplantiran bubreg u KliniÄkoj bolnici Merkur od lipnja 2007. do kraja 2018. god. Ishodi transplantacije praÄeni su do 31. 12. 2019. godine. NajkraÄe vrijeme praÄenja bila je jedna godina. Podaci su prikupljeni koriÅ”tenjem izvjeÅ”Äa iz aplikacije Eurotransplant Network Information System (ENIS; www.eurotransplant.org). Preživljenje je prikazano Kaplan-Meierovim krivuljama. Povezanost preživljenja s odreÄenim obilježjima primatelja i darivatelja analizirana je univarijatnom i multivarijatnom Coxovom regresijom. Rezultati: U razdoblju od lipnja 2007. do konca 2018. presaÄeno je 480 bubrega u 472 pacijenta. 10-godiÅ”nje preživljenje pacijenata iznosilo je 72 %. DesetgodiÅ”nje preživljenje bubrega cenzurirano za smrt pacijenata s bubregom u funkciji bilo je 93 %. U multivarijatnoj analizi jedino dob primatelja pri transplantaciji, Å”eÄerna bolest kao uzrok osnovne bubrežne bolesti i trajanje lijeÄenja dijalizom ostali su neovisno povezani s preživljenjem pacijenata. ZakljuÄak: Transplantacija bubrega rezultira odliÄnim dugoroÄnim preživljenjem bubrega. Potrebno je poboljÅ”ati dugoroÄno preživljenje pacijenata, prevencijom, ranim otkrivanjem i intenzivnim lijeÄenjem kroniÄnih bolesti.Aim: Although kidney transplantation is the best method of replacing renal function, there is still a need to improve long-term outcomes. The aim of this study was to determine the independent association of recipient and donor demographic factors, underlying renal disease, duration of dialysis treatment, tissue typing mismatch, and sensitization with transplant outcomes in a contemporary cohort of kidney transplant patients. Patients and methods: The study included patients who had a kidney transplantation at Clinical Hospital Merkur from June 2007 to the end of 2018. Transplant outcomes were monitored until December 31, 2019. The minimum follow-up time was 1 year. Data were collected using reports from the Eurotransplant Network Information System (ENIS) application (www.eurotransplant.org). Survival is shown by Kaplan-Meier curves. The association of survival with specific recipient and donor characteristics was analyzed by univariate and multivariate Cox regression. Results: In the period from June 2007 to the end of 2018, 480 kidneys were transplanted in 472 patients. The 10-year patient survival was 72%. Ten-year renal survival censored for the death of renal function patients was 93%. In the multivariate analysis, only recipient age at transplantation, diabetes as the cause of underlying renal disease and duration of dialysis remained independently associated with patient survival. Conclusion: Long-term graft survival is excellent after kidney transplantation. Long-term patient survival can be improved by prevention, early detection and intensive treatment of chronic diseases
Pathohistological approach to liver transplantation
Jetra je vitalni organ Äovjeka. Ima važnu ulogu u metabolizmu hranjivih tvari i lijekova, sintezi proteina i filtraciji krvi. Zbog svoje složene graÄe i mnogobrojnih funkcija jetra je Äesto sijelo bolesti. Postoje mnogobrojna patoloÅ”ka stanja jetre, no u kontekstu transplantacijske patologije najbitnija su ona koja dovode do akutnog zatajenja jetre, kroniÄnog zatajenja jetre i ciroze. Ove bolesti lijeÄe se transplantacijom jetre. Transplantacijska patologija bavi se bolestima koje se pojavljuju na alotransplantatu: prezervacijsko oÅ”teÄenje, vaskularne komplikacije, komplikacije žuÄnih vodova, odbacivanje organa, infekcije i recidivirajuÄe bolesti. Prezervacijsko oÅ”teÄenje nastaje kao posljedica produljenog vremena tople i hladne ishemije. Vaskularne komplikacije i komplikacije žuÄnih vodova najÄeÅ”Äe su posljedica kirurÅ”kih komplikacija. Infekcije hepatitis B virusom i hepatitis C virusom Äesto recidiviraju i progresivno vode do zatajenja alotransplantata. Odbacivanje organa dijeli se na humoralno, akutno i kroniÄno. RecidivirajuÄe bolesti ukljuÄuju infektivne, imunoloÅ”ke, tumorske i toksiÄne bolesti jetre. Glavna dijagnostiÄka metoda veÄine ovih stanja je biopsija jetre i patohistoloÅ”ki pregled tkiva. MeÄutim mikroskopska slika ovih bolesti Äesto je nespecifiÄna i vrlo isprepletena. Neke bolesti posebno je teÅ”ko razlikovati samo na temelju mikroskopske slike. Zbog toga je potrebno poznavati vrijeme proteklo od transplantacije, kliniÄku sliku bolesti i stanje pacijenta te Å”iroku diferencijalnu dijagnozu koja dolazi u obzir.The liver is a vital human organ. It has an important role in nutrient and drug metabolism, protein synthesis and blood filtration. Because of its complex structure and multiple functions the liver is a common site of diseases. There are lots of possible pathological states of the liver, but in the context of liver transplantation pathology the most important are: acute liver failure, chronic liver failure and cirrhosis. These diseases are treated with liver transplantation. Transplantation pathology covers diseases that occur on the allograft: preservation injury, vascular complications, bile duct complications, rejection, infections and reccurent diseases. Preservation injury occurs due to prolonged cold and hot ischemia. Vascular complications and bile duct complications are usually a result of surgical complications. Hepatitis B and hepatitis C infections commonly recur in the allograft and lead to progressive allograft failure. Liver rejection is divided into humoral, acute and chronic. Recurrent diseases include infective, immune, neoplastic and toxic liver diseases. The main diagnostic method for most of these diseases is liver biopsy and pathohistological tissue examination. However the microscopic findings can be unspecific and overlapping. Some diseases can be difficult to distinguish based on microscopic findings alone. Therefore it is important to know the time after transplantation, the clinical presentation of the disease and the broad differential diagnosis that needs to be considere
LACTATE DEHYDROGENASE GENE GENOTYPIZATION FOR SPECIES IDENTIFICATION IN A FISH FARM ON THE RIVER NERETVA
There are severale Salmonid species, found in the river Neretva basin, among which S. trutta and S. obtusirostris. Also, natural hybrids such as S. obtusirostris x S. trutta have been observed. In one fish farm on the river Neretva, S. trutta and S. obtusirostris were decided to breed separately. Parental fishes were separated phenotypicaly on the basis of the morphological signs. PCR-RFLP analysis of the exon 3 to exon 4 part of the lactate dehydrogenase (LDH) C1* gene with restriction endonuclease RsaI was employed to identify the presence of other species representatives or intercrosses in two groups of juvenille fishes. Using this method, we were able to identify two S. trutta representatives in the S. obtusirostris group
Real-life experience with remdesivir for treatment of hospitalized coronavirus disease 2019 patients: matched case-control study from a large tertiary hospital registry
Aim: To evaluate the association of remdesivir use and the survival of hospitalized patients with coronavirus disease 2019 (COVID-19).
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Methods: We retrospectively reviewed the medical records of 5959 COVID-19 patients admitted to our tertiary-level hospital from March 2020 to June 2021. A total of 876 remdesivir-treated patients were matched with 876 control patients in terms of age, sex, Charlson comorbidity index (CCI), WHO-defined COVID-19 severity on admission, and oxygen requirement at the time of remdesivir use.
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Results: Among 1752 COVID-19 patients (median age 66 years, 61.8% men), 1405 (80.2%) had severe and 311 (17.8%) had critically severe COVID-19 on admission. Remdesivir was given at a median of one day after hospital admission and at a median of eight days from the onset of symptoms. Overall, 645 (73.6%) patients received remdesivir before high-flow oxygen therapy (HFOT) or mechanical ventilation (MV), 198 (22.6%) after HFOT institution, and 83 (9.5%) after MV institution. Remdesivir use was associated with improved survival in the entire cohort (hazard ratio 0.79, P=0.006). Survival benefit was evident among patients receiving remdesivir during low-flow oxygen requirement (hazard ratio 0.61, P<0.001) but not among patients who received it after starting HFOT (P=0.499) or MV (P=0.380).
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Conclusion: Remdesivir, if given during low-flow oxygen therapy, might be associated with survival benefit in hospitalized COVID-19 patients