15,502 research outputs found
Plant Cell Wall Hydration and Plant Physiology: An Exploration of the Consequences of Direct Effects of Water Deficit on the Plant Cell Wall
The extensibility of synthetic polymers is routinely modulated by the addition of lower molecular weight spacing molecules known as plasticizers, and there is some evidence that water may have similar effects on plant cell walls. Furthermore, it appears that changes in wall hydration could affect wall behavior to a degree that seems likely to have physiological consequences at water potentials that many plants would experience under field conditions. Osmotica large enough to be excluded from plant cell walls and bacterial cellulose composites with other cell wall polysaccharides were used to alter their water content and to demonstrate that the relationship between water potential and degree of hydration of these materials is affected by their composition. Additionally, it was found that expansins facilitate rehydration of bacterial cellulose and cellulose composites and cause swelling of plant cell wall fragments in suspension and that these responses are also affected by polysaccharide composition. Given these observations, it seems probable that plant environmental responses include measures to regulate cell wall water content or mitigate the consequences of changes in wall hydration and that it may be possible to exploit such mechanisms to improve crop resilience
Beyond recurrent costs: an institutional analysis of the unsustainability of donor-supported reforms in agricultural extension
International donors have spent billions of dollars over the past four decades in developing and/or reforming the agricultural extension service delivery arrangements in developing countries. However, many of these reforms, supported through short-term projects, became unsustainable once aid funding had ceased. The unavailability of recurrent funding has predominantly been highlighted in the literature as the key reason for this undesirable outcome, while little has been written about institutional factors. The purpose of this article is to examine the usefulness of taking an institutional perspective in explaining the unsustainability of donor-supported extension reforms and derive lessons for improvement. Using a framework drawn from the school of institutionalism in a Bangladeshi case study, we have found that a reform becomes unsustainable because of poor demands for extension information and advice; missing, weak, incongruent, and perverse institutional frameworks governing the exchange of extension goods (services); and a lack of institutional learning and change during the reform process. Accordingly, we have argued that strategies for sustainable extension reforms should move beyond financial considerations and include such measures as making extension goods (services) more tangible and monetary in nature, commissioning in-depth studies to learn about local institutions, crafting new institutions and/or reforming the weak and perverse institutions prevailing in developing countries. We emphasize the need to address three categories of institutions – regulative, normative, and cultural-cognitive – and call for an alignment among them. We further argue that, in order to be sustainable, a reform should take a systemic approach in institutional capacity building and, for this to be possible, adopt a long-term program approach, as opposed to a short-term project approach
Deep-Elastic pp Scattering at LHC from Low-x Gluons
Deep-elastic pp scattering at c.m. energy 14 TeV at LHC in the momentum
transfer range 4 GeV*2 < |t| < 10 GeV*2 is planned to be measured by the TOTEM
group. We study this process in a model where the deep-elastic scattering is
due to a single hard collision of a valence quark from one proton with a
valence quark from the other proton. The hard collision originates from the
low-x gluon cloud around one valence quark interacting with that of the other.
The low-x gluon cloud can be identified as color glass condensate and has size
~0.3 F. Our prediction is that pp differential cross section in the large |t|
region decreases smoothly as momentum transfer increases. This is in contrast
to the prediction of pp differential cross section with visible oscillations
and smaller cross sections by a large number of other models.Comment: 10 pages, including 4 figure
Microarray screening of Guillain-Barré syndrome sera for antibodies to glycolipid complexes
Objective: To characterize the patterns of autoantibodies to glycolipid complexes in a large cohort of Guillain-Barré syndrome (GBS) and control samples collected in Bangladesh using a newly developed microarray technique.
Methods: Twelve commonly studied glycolipids and lipids, plus their 66 possible heteromeric complexes, totaling 78 antigens, were applied to polyvinylidene fluoride–coated slides using a microarray printer. Arrays were probed with 266 GBS and 579 control sera (2 μL per serum, diluted 1/50) and bound immunoglobulin G detected with secondary antibody. Scanned arrays were subjected to statistical analyses.
Results: Measuring antibodies to single targets was 9% less sensitive than to heteromeric complex targets (49.2% vs 58.3%) without significantly affecting specificity (83.9%–85.0%). The optimal screening protocol for GBS sera comprised a panel of 10 glycolipids (4 single glycolipids GM1, GA1, GD1a, GQ1b, and their 6 heteromeric complexes), resulting in an overall assay sensitivity of 64.3% and specificity of 77.1%. Notable heteromeric targets were GM1:GD1a, GM1:GQ1b, and GA1:GD1a, in which exclusive binding to the complex was observed.
Conclusions: Rationalizing the screening protocol to capture the enormous diversity of glycolipid complexes can be achieved by miniaturizing the screening platform to a microarray platform, and applying simple bioinformatics to determine optimal sensitivity and specificity of the targets. Glycolipid complexes are an important category of glycolipid antigens in autoimmune neuropathy cases that require specific analytical and bioinformatics methods for optimal detection
Matrix Adhesion Polarizes Heart Progenitor Induction In The Invertebrate Chordate Ciona Intestinalis
Cell-matrix adhesion strongly influences developmental signaling. Resulting impacts on cell migration and tissue morphogenesis are well characterized. However, the in vivo impact of adhesion on fate induction remains ambiguous. Here, we employ the invertebrate chordate Ciona intestinalis to delineate an essential in vivo role for matrix adhesion in heart progenitor induction. In Ciona pre-cardiac founder cells, invasion of the underlying epidermis promotes localized induction of the heart progenitor lineage. We found that these epidermal invasions are associated with matrix adhesion along the pre-cardiac cell/epidermal boundary. Through targeted manipulations of RAP GTPase activity, we were able to manipulate pre-cardiac cell-matrix adhesion. Targeted disruption of pre-cardiac cell-matrix adhesion blocked heart progenitor induction. Conversely, increased matrix adhesion generated expanded induction. We were also able to selectively restore cell-matrix adhesion and heart progenitor induction through targeted expression of Ci-Integrin β2. These results indicate that matrix adhesion functions as a necessary and sufficient extrinsic cue for regional heart progenitor induction. Furthermore, time-lapse imaging suggests that cytokinesis acts as an intrinsic temporal regulator of heart progenitor adhesion and induction. Our findings highlight a potentially conserved role for matrix adhesion in early steps of vertebrate heart progenitor specification
Pressure-Induced Simultaneous Metal-Insulator and Structural-Phase Transitions in LiH: a Quasiparticle Study
A pressure-induced simultaneous metal-insulator transition (MIT) and
structural-phase transformation in lithium hydride with about 1% volume
collapse has been predicted by means of the local density approximation (LDA)
in conjunction with an all-electron GW approximation method. The LDA wrongly
predicts that the MIT occurs before the structural phase transition. As a
byproduct, it is shown that only the use of the generalized-gradient
approximation together with the zero-point vibration produces an equilibrium
lattice parameter, bulk modulus, and an equation of state that are in excellent
agreement with experimental results.Comment: 7 pages, 4 figures, submitted to Europhysics Letter
Weak-Light Ultraslow Vector Optical Solitons via Electromagnetically Induced Transparency
We propose a scheme to generate temporal vector optical solitons in a
lifetime broadened five-state atomic medium via electromagnetically induced
transparency. We show that this scheme, which is fundamentally different from
the passive one by using optical fibers, is capable of achieving
distortion-free vector optical solitons with ultraslow propagating velocity
under very weak drive conditions. We demonstrate both analytically and
numerically that it is easy to realize Manakov temporal vector solitons by
actively manipulating the dispersion and self- and cross-phase modulation
effects of the system.Comment: 4 pages, 4 figure
Magnetic field induced orientation of superconducting MgB crystallites determined by X-ray diffraction
X-ray diffraction studies of fine polycrystalline samples of MgB in the
superconducting state reveal that crystals orient with their \emph{c}-axis in a
plane normal to the direction of the applied magnetic field. The MgB
samples were thoroughly ground to obtain average grain size 5 - 10 m in
order to increase the population of free single crystal grains in the powder.
By monitoring Bragg reflections in a plane normal to an applied magnetic field
we find that the powder is textured with significantly stronger (\emph{0,0,l})
reflections in comparison to (\emph{h,k,0}), which remain essentially
unchanged. The orientation of the crystals with the \emph{ab}-plane parallel to
the magnetic field at all temperatures below demonstrates that the sign
of the torque under magnetic field does not alter, in disagreement with current
theoretical predictions
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