Effect of plant growth regulators on in vitro shoot multiplication of Amygdalus communis L. cv. Yaltsinki

An efficient protocol was established for in vitro shoot multiplication from apical shoot tips derived from mature trees of almond (Amygdalus communis L.) cultivars, Yaltsinki. Explants were cultured on Murashige and Skoog (1962) (MS) medium containing various concentrations of 6-benzyladenin (BA) and kinetin (kin) for shoot multiplication. Shoot multiplication was best achieved from explant on MS medium containing 30 gl-1 sucrose, 7 gl-1 agar and 1.0 mgl-1 BA. This amount of BA (1.0 mgl-1) gave the best multiple shoot formation response with an average of 16.10 shoots per explant. In addition, shoots were cultured on the media containing 1.0 mgl-1 BA and kin combined with three different auxins (0.25 and 0.5 mgl-1 of IAA, IBA and NAA) separately. It was noted that 1.0 mgl-1 BA and kin combinated with NAA had inhibitory effect on new shoot formation and no shoot formation was induced. However, explants cultivated on medium containing 1.0 mgl-1 BA and 0.5 mgl-1 IAA resulted in 11.25 shoots per explant. The effect of four different sucrose concentrations (20, 30, 40, 50 gl-1) on the multiplication of shoots was also investigated. The best shoot multiplication was obtained in MS media containing 30 gl-1 sucrose with an average of 15.40 shoots per explant

Studies on accumulation of Mn2+ by Nasturtium officinale in water by biosorption

In present study, Nasturtium officinale has been used as a plant. The plant has been collected from the campus of Dicle university. The study has been achieved in vitro. Air roots of the plant with the same number and morphology of leaf has been chosen. For biosorption of Mn2+ metal ions by Nasturtium officinale, the solutions were prepared as 0.1; 0.3; 0.5; 1; 2; 3; 4 and 5 ppm, respectively. Water samples has been prepared from 1000 ppm stock and put them to balon joje. After this process, the plants have been transferred to the erlens and wait for 24,48 and 72 h. The erlens have been tightly closed with parafilm. The general appearence of the plants have been observed at the beginning and after 24 h. The plants has been analyzed with atomic absorption spectrometry by receiving 2 mL of water samples for calculation of concentration. The A and B values have been found by drawing the calibration lines in UV. The wet weight has been measured with sensitive balance. The quantity of Mn2+ absorption of plants has been calculated with the dependence of the wet weight

Biosorption

In present study, Nasturtium officinale has been used as a plant. The plant has been collected from the campus of Dicle university. The study has been achieved in vitro. Air roots of the plant with the same number and morphology of leaf has been chosen. For biosorption of Mn2+ metal ions by Nasturtium officinale, the solutions were prepared as 0.1; 0.3; 0.5; 1; 2; 3; 4 and 5 ppm, respectively. Water samples has been prepared from 1000 ppm stock and put them to balon joje. After this process, the plants have been transferred to the erlens and wait for 24,48 and 72 h. The erlens have been tightly closed with parafilm. The general appearence of the plants have been observed at the beginning and after 24 h. The plants has been analyzed with atomic absorption spectrometry by receiving 2 mL of water samples for calculation of concentration. The A and B values have been found by drawing the calibration lines in UV. The wet weight has been measured with sensitive balance. The quantity of Mn2+ absorption of plants has been calculated with the dependence of the wet weight

In vitro micropropagation of almond (Amygdalus communis L. cv. Nonpareil)

An efficient in vitro propagation method was developed for almond (Amygdalus communis L. cv. Nonpareil). The effect of BA and kinetin (0.0, 0.5, 1.0, 2.0, 4.0 mgl-1) on the culture initiation of zygoticembryos isolated from mature seeds was investigated. A Murashige and Skoog (1962) (MS) medium containing 30 gl-1 sucrose, 0.5 and 1.0 mgl-1 N6-benzylaminopurine (BA) and 7 gl-1 agar resulted in amultiple shoot initiation at the rate of 11.0 ± 1.32 and 14.7 ± 2.12 shoot per explant, respectively, in 28 days of culture. The effects of a low concentration of BA (0.1, 0.5, 1.0 and 2.0 mgl-1) and differentcombinations of auxin + cytokinin were investigated for shoot proliferation. The best results for new shoot production were obtained from a MS culture medium which was supplemented with 1.0 mgl-1 BA.The rooting was achieved in a ½ MS medium supplemented with 8.0 mgl-1 indole acetic acid (IAA). The in vitro raised plants were acclimatized in a growth room and successfully transplanted to the field.This method here in described will be useful for the rapid multiplication of almond (A. communis L. cv. Nonpareil) in commercial exploitation

The relationship between inflammation markers and ketonuria in hyperemesis gravidarum

Objective Hyperemesis gravidarum is an illness that starts in early pregnancy and manifests itself with oral intake problems, electrolyte imbalance, ketonuria, and weight loss. Inflammation is closely associated with the hyperemesis gravidarum, and inflammatory indicators have been studied to understand its pathophysiology. This study investigates the relationship of ketonuria levels with inflammatory markers platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR), and neutrophil-to-lymphocyte ratio (NLR) for hyperemesis gravidarum patients