61 research outputs found
Determination of water content in clay and organic soil using microwave oven
The article deals with the techniques of soil water content determination using microwave radiation. Its practical application would allow solving the problems of resource efficiency in geotechnical survey due to reduction of energy and resource intensity of laboratory analysis as well as its acceleration by means of decreasing labour intensity and, as a result, cost reduction. The article presents a detail analysis of approaches to soil water content determination and soil drying, considers its features and application. The study in soil of different composition, typical for Western Siberia including organic and organic-mineral ones, is a peculiarity of the given article, which makes it rather topical. The article compares and analyzes the results of the investigation into soil water content, which are obtained via conventional techniques and the original one developed by the authors, consisting in microwave drying. The authors also give recommendation on microwave technique application to dry soil
Investigation of interaction of carbon dioxide with aerogels nanopores
The absorption spectrum of 2 0 0 12 β 0 0 0 01 band of carbon dioxide, confined in 20 nm nanopores of silica aerogel, was measured with help of a Bruker IFS 125 HR Fourier transform spectrometer at room temperature and a spectral resolution of 0.01 cm-1. The obtained dependence of spectral line half-width values on rotational quantum numbers was studied and compared with data available in the literature. Β© (2015) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only
Hantaviirusinfektsioonid Eestis
Hantaviirusnakkused on zoonootilised β viirused tsirkuleerivad looduses ilma inimese osaluseta, neid levib nΓ€riliste vahendusel. Tuntud on kaks kliiniliselt erinevat haigusvormi β neerusΓΌndroomiga hemorraagiline palavik (levinud Aasias ja Euroopas) ning hantaviiruse pulmonaalne sΓΌndroom (levinud Ameerikas). SΓ΅ltuvalt viirustΓΌΓΌbist vΓ΅ib letaalsus haigestumuse korral ulatuda kuni 40%-ni. Eestis on isoleeritud Dobrava genotΓΌΓΌp viirus. Saaremaal pΓΌΓΌtud juttselg hiirtel on isoleeritud viirus osutunud aga omaette genotΓΌΓΌpi kuuluvaks ja on nimetatud saaremaa hantaviiruseks. Hantaviirusnakkus on levinud ka Eestis β aastal 2001 esines 12 seroloogiliselt kinnitatud haigusjuht
Characterization of Hemorrhagic Fever with Renal Syndrome Caused by Hantaviruses, Estonia
Thirty cases of hemorrhagic fever with renal syndrome (HFRS) due to Puumala virus (PUUV), Saaremaa virus (SAAV), and Dobrava virus infection were confirmed in Estonia. Except for the levels of serum creatinine, no remarkable differences were found in the clinical course of HFRS caused by PUUV and SAAV
Electrochemical Synthesis of Zirconium Pre-Catalysts for Homogeneous Ethylene Oligomerization
The catalytic activity of electrochemically synthesized zirconium carboxylates was studied in the process of ethylene oligomerization. Zirconium carboxylates were electrochemically synthesized directly from metallic zirconium and corresponding carboxylic acids (acetic, octanoic and lauric). A comprehensive study (element analysis, nuclear magnetic resonance (NMR) and infrared (IR) spectroscopy, powder X-ray diffraction (PXRD)) of the synthesized zirconium carboxylates showed that these species contain bidentate carboxylate moieties. It was shown that obtained zirconium carboxylates, in combination with Et3Al2Cl3 (Al/Zr = 20), have a moderate activity of (7.6-9.9) Γ 103 molC2H4β
molZr-1β
h-1 in terms of ethylene oligomerization (at T = 80 ?C, p = 20 bar), leading to even-numbered C4-C10 linear alpha-olefins.1059-105
REACTIVITY OF RAT ERYTHROCYTES UNDER CONDITIONS OF PHYSICAL STRESS OF DIFFERENT INTENSITY
Π¦Π΅Π»Ρ. ΠΠ·ΡΡΠΈΡΡ ΠΌΠΎΡΡΠΎΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ΅ ΡΠΎΡΡΠΎΡΠ½ΠΈΠ΅ ΠΊΡΠ°ΡΠ½ΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ ΠΊΡΡΡ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π°Π³ΡΡΠ·ΠΊΠΈ Β«Π΄ΠΎ ΠΎΡΠΊΠ°Π·Π°Β».ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
ΠΏΠΎΠ»ΠΎΠ²ΠΎΠ·ΡΠ΅Π»ΡΡ
ΠΊΡΡΡ-ΡΠ°ΠΌΡΠΎΠ² Wistar: ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ, Π½Π°Ρ
ΠΎΠΆΠ΄Π΅Π½ΠΈΠ΅ Π² Π²ΠΎΠ΄Π΅ Π±Π΅Π· Π΄Π²ΠΈΠΆΠ΅Π½ΠΈΡ, ΡΠ²ΠΎΠ±ΠΎΠ΄Π½ΠΎΠ΅ ΠΏΠ»Π°Π²Π°Π½ΠΈΠ΅ ΠΈ ΠΏΠ»Π°Π²Π°Π½ΠΈΠ΅ Ρ Π³ΡΡΠ·ΠΎΠΌ 2%, 6%, 8%, 10% ΠΈ 15% ΠΎΡ ΠΌΠ°ΡΡΡ ΡΠ΅Π»Π° Β«Π΄ΠΎ ΠΎΡΠΊΠ°Π·Π°Β» ΡΠΎΠ³Π»Π°ΡΠ½ΠΎ ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Ρ ΠΏΠΎ Π±ΠΈΠΎΡΡΠΈΠΊΠ΅ ΡΡΠ²Π΅ΡΠΆΠ΄Π΅Π½Π½ΠΎΠΌΡ ΠΠ€ ΠΠΎΠΌΠΈ ΠΠ¦ Π£ΡΠ Π ΠΠ. Π ΠΊΡΠΎΠ²ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΡΡΠΎΠ²Π΅Π½Ρ Π³Π΅ΠΌΠΎΠ³Π»ΠΎΠ±ΠΈΠ½Π°, Π³Π΅ΠΌΠ°ΡΠΎΠΊΡΠΈΡΠ°, ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² ΠΈ ΡΠ΅ΡΠΈΠΊΡΠ»ΠΎΡΠΈΡΠΎΠ². ΠΠ·ΠΌΠ΅ΡΡΠ»ΠΈ Π΄ΠΈΠ°ΠΌΠ΅ΡΡ 100 ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² ΠΎΠΊΡΠ°ΡΠ΅Π½Π½ΡΡ
ΠΏΠΎ Π ΠΎΠΌΠ°Π½ΠΎΠ²ΡΠΊΠΎΠΌΡ-ΠΠΈΠΌΠ·Π° ΠΈ Π±ΡΠΈΠ»Π»ΠΈΠ°Π½ΡΠΎΠ²ΡΠΌ ΠΊΡΠ΅Π·ΠΈΠ»ΠΎΠ²ΡΠΌ ΡΠΈΠ½ΠΈΠΌ. Π‘ΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΡΡ Π·Π½Π°ΡΠΈΠΌΠΎΡΡΡ ΡΠ°Π·Π»ΠΈΡΠΈΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΉ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π½Π΅ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΡΠΈΡΠ΅ΡΠΈΡ ΠΡΡΡΠΊΠ°Π»Π°-Π£ΠΎΠ»Π»ΠΈΡΠ°.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π€ΠΈΠ·ΠΈΡΠ΅ΡΠΊΠ°Ρ Π½Π°Π³ΡΡΠ·ΠΊΠ° Β«Π΄ΠΎ ΠΎΡΠΊΠ°Π·Π°Β» Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π»Π°ΡΡ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ΠΌ Π²Π΅Π»ΠΈΡΠΈΠ½Ρ Π³Π΅ΠΌΠ°ΡΠΎΠΊΡΠΈΡΠ°, Π³Π΅ΠΌΠΎΠ³Π»ΠΎΠ±ΠΈΠ½Π°, ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎΠΌ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² Π² ΠΊΡΠΎΠ²ΠΈ ΠΊΡΡΡ ΠΈ ΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΠ΅ΡΠΈΠΊΡΠ»ΠΎΡΠΈΡΠΎΠ·ΠΎΠΌ, Π½Π°ΡΡΠ΄Ρ ΡΠΎ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Π³Π΅ΠΌΠΎΠ³Π»ΠΎΠ±ΠΈΠ½Π° Π² ΡΡΠΈΡΡΠΎΡΠΈΡΠ΅. ΠΡΠΌΠ΅ΡΠ΅Π½ΠΎ, ΡΡΠΎ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠ΅ΡΠΈΠΊΡΠ»ΠΎΡΠΈΡΠΎΠ² ΠΏΠΎ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΡ Π² ΠΏΡΠ΅Π΄Π΅Π»Π°Ρ
6.4β6.7 ΠΌΠΊΠΌ Ρ ΠΏΠ»Π°Π²Π°ΡΡΠΈΡ
ΠΊΡΡΡ Ρ Π³ΡΡΠ·ΠΎΠΌ 8% ΠΎΡ ΠΌΠ°ΡΡΡ ΡΠ΅Π»Π° ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΠ΅Ρ ΠΊΡΠΈΠ²ΠΎΠΉ ΠΡΠ°ΠΉΡ-ΠΠΆΠΎΠ½ΡΠ° ΠΈΠ½ΡΠ°ΠΊΡΠ½ΡΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π£ ΠΊΡΡΡ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ° ΠΈ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠΈΠ·ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π°Π³ΡΡΠ·ΠΊΠΈ ΠΏΡΠΎΡΠ²ΠΈΠ»ΠΈΡΡ ΡΠ°Π·Π½ΠΎΠ½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠ΅ ΡΠ΄Π²ΠΈΠ³ΠΈ Π² ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΌ ΡΠΎΡΡΠ°Π²Π΅ ΠΊΡΠ°ΡΠ½ΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ. ΠΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΎΠ»ΡΠΊΠΎ ΡΠ΅ΡΡΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠ»Π°Π²Π°Π½ΠΈΡ ΠΊΡΡΡ Ρ Π³ΡΡΠ·ΠΎΠΌ 8 % ΠΎΡ ΠΌΠ°ΡΡΡ ΡΠ΅Π»Π°, ΡΡΠ΄Ρ ΠΏΠΎ ΡΠ΅Π°ΠΊΡΠΈΠΈ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ², ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ ΠΎΠ±ΠΎΡΠ½ΠΎΠ²Π°Π½Π½ΡΠΌ.Background. To study the morphofunctional state of red blood in rats depending on the physical load βto failureβ.Materials and methods. The study used practically healthy adult male rats of the Wistar: control, staying in water without movement, free swimming and swimming with a load of 2%, 6%, 8%, 10% and 15% of body weight βto failureβ according to the protocol on bioethics approved by the IPhys Komi SC UB RAS. In the blood, the level of hemoglobin, hematocrit, the number of erythrocytes and reticulocytes was determined. The diameter of 100 erythrocytes stained according to Romanovsky-Giemsa and diamond cresyl blue was measured. The statistical significance of the differences was assessed using the nonparametric Kruskal-Wallis criterion.Results. The physical load Β«to failureΒ» was characterized by an increase in hematocrit, hemoglobin, and the number of erythrocytes in the blood of rats and physiological reticulocytosis, along with a decrease in the concentration of hemoglobin in the erythrocyte. It was noted that the distribution of reticulocytes of diameters within 6.4β6.7 ΞΌm in floating rats with a weight of 8% of body weight corresponds to the Price-Jones curve of intact animals.Conclusion. In rats, depending on the intensity of exercise, multidirectional shifts in the cellular composition of red blood appeared. Practical use only of the test swimming of rats with a weight of 8% of body weight, judging by the nature of the reaction of erythrocytes, is physiologically justified
S-layer protein 2 of 'Lactobacillus crispatus' 2029, its structural and immunomodulatory characteristics and roles in protective potential of the whole bacteria against foodborne pathogens
We have previously demonstrated that human vaginal Lactobacillus crispatus 2029 (LC2029) strain is highly adhesive to cervicovaginal epithelial cells, exhibits antagonistic activity against genitourinary pathogens and expresses surface-layer protein (Slp). The aims of the present study were elucidation of Slp structural and immunomodulatory characteristics
and its roles in protective properties of the whole vaginal LC2029 bacteria against foodborne pathogens. Enteric Caco-2 and colon HT-29 cell lines were used as the in vitro models of the human intestinal epithelial layer. LC2029 strain has two homologous surface-layer (S-layer) genes, slp1 and slp2. Whilst we found no evidence for the expression of slp1 under the
growth conditions used, a very high level of expression of the slp2 gene was detected. C-terminal part of the amino sequence of Slp2 protein was found to be highly similar to that of
the conserved C-terminal region of SlpA protein of L. crispatus Zj001 isolated from pig intestines and CbsA protein of L. crispatus JCM5810 isolated from chicken intestines, and
was substantially variable at the N-terminal and middle regions. The amino acid sequence identity between SlpA and CbsA was as high as 84%, whilst the identity levels of these sequences with that of Slp2 were only 49% and 50% (respectively). LC2029 strain was found to be both acid and bile tolerant. Survival in simulated gastric and intestinal juices of LC2029
cells unable to produce Slp2 was reduced by 2-3 logs. Vaginal L. crispatus 1385 (LC1385) strain not expressing Slp was also very sensitive to gastric and intestinal stresses. Slp2 was
found to be non-covalently bound to the surface of the bacterium, acting as an adhesin and facilitating interaction of LC2029 lactobacilli with the host immature or fully differentiated Caco-2 cells, as well as HT-29 cells. No toxicity to or damage of Caco-2 or HT-29 epithelial cells were detected after 24 h of colonization by LC2029 lactobacilli. Both Slp2 protein and LC2029 cells induced NF-kB activation in Caco-2 and HT-29 cells, but did not induce expression of innate immunity mediators Il-8, Il-1Ξ², and TNF-Ξ±. Slp2 and LC2029 inhibited Il-8 production in Caco-2 and HT-29 cells induced by MALP-2 and increased production of anti-inflammatory cytokine Il-6. Slp2 inhibited production of CXCL1 and RANTES by
Caco-2 cells during differentiation and maturation process within 15 days. Culturing Caco-2 and HT-29 cells in the presence of Slp2 increased adhesion of bifidobacteria BLI-2780 to these enterocytes. Upon binding to Caco-2 and HT-29 cells, Slp2 protein and LC2029 lactobacilli were recognized by toll-like receptors (TLR) 2/6. It was shown that LC2029 strain is a strong co-aggregator of foodborne pathogens Campylobacter jejuni, Salmonella enteritidis, and Escherichia coli O157:H used in this study. The Slp2 was responsible for the ability of LC2029 to co-aggregate these enteropathogens. Slp2 and intact LC2029 lactobacilli inhibited foodborne pathogen-induced activation of caspase-9 and caspase-3 as apoptotic biomarkers in Caco-2 and HT-29 cells. In addition, Slp2 and Slp2-positive LC2029 strain
reduced adhesion of tested pathogenic bacteria to Caco-2 and HT-29 cells. Slp2-positive LC2029 strain but not Slp2 alone provided bactericidal effect on foodborne pathogens. These
results suggest a range of mechanisms involved in inhibition of growth, viability, and cell adhesion properties of pathogenic Proteobacteria by the Slp2 producing LC2029, which may
be useful in treatment of necrotizing enterocolitis (NEC) in newborns and foodborne infectious diseases in children and adults, increasing the colonization resistance and maintaining the intestinal homeostasis
'Limosilactobacillus fermentum' Strain 3872 : antibacterial and immunoregulatory properties and synergy with prebiotics against socially significant antibiotic-resistant infections of animals and humans
Limosilactobacillus fermentum strain 3872 (LF3872) was originally isolated from the breast milk of a healthy woman during lactation and the breastfeeding of a child. The high-quality genome sequencing of LF3872 was performed, and a gene encoding a unique bacteriocin was discovered. It was established that the bacteriocin produced by LF3872 (BLF3872) belongs to the family of cell-wall-degrading proteins that cause cell lysis. The antibacterial properties of LF3872 were studied using test cultures of antibiotic-resistant Gram-positive and Gram-negative pathogens. Gram-positive pathogens (Staphylococcus aureus strain 8325-4 and S. aureus strain IIE CI-SA 1246) were highly sensitive to the bacteriolytic action of LF3872. Gram-negative pathogens (Escherichia coli, Salmonella strains, and Campylobacter jejuni strains) were more resistant to the bacteriolytic action of LF3872 compared to Gram-positive pathogens. LF3872 is a strong co-aggregator of Gram-negative pathogens. The cell-free culture supernatant of LF3872 (CSLF3872) induced cell damage in the Gram-positive and Gram-negative test cultures and ATP leakage. In the in vitro experiments, it was found that LF3872 and Actigen prebiotic (Alltech Inc., Nicholasville, KY, USA) exhibited synergistic anti-adhesive activity against Gram-negative pathogens. LF3872 has immunoregulatory properties: it inhibited the lipopolysaccharide-induced production of proinflammatory cytokines IL-8, IL-1Ξ², and TNF-Ξ± in a monolayer of Caco-2 cells; inhibited the production of IL-12 and stimulated the production of IL-10 in immature human dendritic cells; and stimulated the production of TGF-Ξ², IFN-Ξ³, and IgA in the immunocompetent cells of intestinal Peyerβs patches (PPs) in mice. These results indicate the possibility of creating a synbiotic based on LF3872 and a prebiotic derived from Saccharomyces cerevisiae cell wall components. Such innovative drugs and biologically active additives are necessary for the implementation of a strategy to reduce the spread of antibiotic-resistant strains of socially significant animal and human infections
Educational transformation in transitional China and Russia : a comparative analysis of private schooling in Beijing and Moscow
This thesis is a comparative study of private schooling in Beijing and Moscow. It studies the educational transformation in transitional China and Russia. This innovative study goes beyond the comparative social, economic and cultural measurement of selected private schools and analyses the role of globalisation in education in these societies, which resulted in the development of the system of private schools
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