A four-dimensional organoid system to visualize cancer cell vascular invasion

Yanagisawa, K.; Konno, M.; Liu, H.; Irie, S.; Mizushima, T.; Mori, M.; Doki, Y.; Eguchi, H.; Matsusaki, M.; Ishii, H. A Four-Dimensional Organoid System to Visualize Cancer Cell Vascular Invasion. Biology 2020, 9, 361

Class I Gap-formation in Highly-viscous Glass-ionomer Restorations: Delayed vs Immediate Polishing

This in vitro study evaluated the effects of delayed versus immediate polishing to permit maturation of interfacial gap-formation around highly viscous conventional glass-ionomer cement (HV-GIC) in Class I restorations, together with determining the associated mechanical properties. Cavity preparations were made on the occlusal surfaces of premolars. Three HV-GICs (Fuji IX GP, GlasIonomer FX-II and Ketac Molar) and one conventional glass-ionomer cement (C-GIC, Fuji II, as a control) were studied, with specimen subgroups (n=10) for each property measured. After polishing, either immediately (six minutes) after setting or after 24 hours storage, the restored teeth were sectioned in a mesiodistal direction through the center of the model Class I restorations. The presence or absence of interfacial-gaps was measured at 1000× magnification at 14 points (each 0.5-mm apart) along the cavity restoration interface (n=10; total points measured per group = 140). Marginal gaps were similarly measured in Teflon molds as swelling data, together with shear-bond-strength to enamel and dentin, flexural strength and moduli. For three HV-GICs and one C-GIC, significant differences (p<0.05) in gap-incidence were observed between polishing immediately and after one-day storage. In the former case, 80–100 gaps were found. In the latter case, only 9–21 gaps were observed. For all materials, their shear-bond-strengths, flexural strength and moduli increased significantly after 24-hour storage.</p

Class I Gap-formation in Highly-viscous Glass-ionomer Restorations: Delayed vs Immediate Polishing

This in vitro study evaluated the effects of delayed versus immediate polishing to permit maturation of interfacial gap-formation around highly viscous conventional glass-ionomer cement (HV-GIC) in Class I restorations, together with determining the associated mechanical properties. Cavity preparations were made on the occlusal surfaces of premolars. Three HV-GICs (Fuji IX GP, GlasIonomer FX-II and Ketac Molar) and one conventional glass-ionomer cement (C-GIC, Fuji II, as a control) were studied, with specimen subgroups (n=10) for each property measured. After polishing, either immediately (six minutes) after setting or after 24 hours storage, the restored teeth were sectioned in a mesiodistal direction through the center of the model Class I restorations. The presence or absence of interfacial-gaps was measured at 1000× magnification at 14 points (each 0.5-mm apart) along the cavity restoration interface (n=10; total points measured per group = 140). Marginal gaps were similarly measured in Teflon molds as swelling data, together with shear-bond-strength to enamel and dentin, flexural strength and moduli. For three HV-GICs and one C-GIC, significant differences (p<0.05) in gap-incidence were observed between polishing immediately and after one-day storage. In the former case, 80–100 gaps were found. In the latter case, only 9–21 gaps were observed. For all materials, their shear-bond-strengths, flexural strength and moduli increased significantly after 24-hour storage.</p

Research on the pathology of Alzheimer\u27s disease based on toxic conformation theory of amyloid β

富山大学・富医薬博甲第428号・牧 俊人・2023/03/23公表論文1.Maki T, Sawahata M, Akutsu I, Amaike S, Hiramatsu G, Uta D, Izuo N, Shimizu T, Irie K, Kume T. APP Knock-In Mice Produce E22P-Aβ Exhibiting an Alzheimer\u27s Disease-like Phenotype with Dysregulation of Hypoxia-Inducible Factor Expression. Int J Mol Sci. 2022 Oct 31;23(21):13259. doi: 10.3390/ijms232113259. PMID: 36362046; PMCID: PMC9654501.2.Maki T, Sawahata M, Uta D, Irie K, Kume T. Chronic Treatment with Aβ42 with a Toxic Conformer and LPS Induces Inflammatory Responses in BV-2 Microglia with Dysregulation of Hypoxia-Inducible Factor Expression. Biol Pharm Bull. 2023;46(2):359-363. doi: 10.1248/bpb.b22-00855. PMID: 36724966.富山大

Influence Maximization Meets Efficiency and Effectiveness: A Hop-Based Approach

Influence Maximization is an extensively-studied problem that targets at selecting a set of initial seed nodes in the Online Social Networks (OSNs) to spread the influence as widely as possible. However, it remains an open challenge to design fast and accurate algorithms to find solutions in large-scale OSNs. Prior Monte-Carlo-simulation-based methods are slow and not scalable, while other heuristic algorithms do not have any theoretical guarantee and they have been shown to produce poor solutions for quite some cases. In this paper, we propose hop-based algorithms that can easily scale to millions of nodes and billions of edges. Unlike previous heuristics, our proposed hop-based approaches can provide certain theoretical guarantees. Experimental evaluations with real OSN datasets demonstrate the efficiency and effectiveness of our algorithms.Comment: Extended version of the conference paper at ASONAM 2017, 11 page

Aspherical Lagrangian submanifolds, Audin's conjecture and cyclic dilations

Given a closed, oriented Lagrangian submanifold $L$ in a Liouville domain $\overline{M}$, one can define a Maurer-Cartan element with respect to a certain $L_\infty$-structure on the string homology $\widehat{H}_\ast^{S^1}(\mathcal{L}L;\mathbb{R})$, completed with respect to the action filtration. When the first Gutt-Hutchings capacity of $\overline{M}$ is finite, and $L$ is a $K(\pi,1)$ space, it leads to interesting geometric implications. In particular, we show that $L$ bounds a non-constant pseudoholomorphic disc of Maslov index 2. This confirms a general form of Audin's conjecture and generalizes the works of Fukaya and Irie in the case of $\mathbb{C}^n$ to a wide class of Liouville manifolds. In particular, when $\dim_\mathbb{R}(\overline{M})=6$, every closed, orientable, prime Lagrangian 3-manifold $L\subset\overline{M}$ is diffeomorphic to a spherical space form, or $S^1\times\Sigma_g$, where $\Sigma_g$ is a closed oriented surface.Comment: 75 pages, 6 figures. v2: slightly simplified the argument, references adde

Sketch-based Influence Maximization and Computation: Scaling up with Guarantees

Propagation of contagion through networks is a fundamental process. It is used to model the spread of information, influence, or a viral infection. Diffusion patterns can be specified by a probabilistic model, such as Independent Cascade (IC), or captured by a set of representative traces. Basic computational problems in the study of diffusion are influence queries (determining the potency of a specified seed set of nodes) and Influence Maximization (identifying the most influential seed set of a given size). Answering each influence query involves many edge traversals, and does not scale when there are many queries on very large graphs. The gold standard for Influence Maximization is the greedy algorithm, which iteratively adds to the seed set a node maximizing the marginal gain in influence. Greedy has a guaranteed approximation ratio of at least (1-1/e) and actually produces a sequence of nodes, with each prefix having approximation guarantee with respect to the same-size optimum. Since Greedy does not scale well beyond a few million edges, for larger inputs one must currently use either heuristics or alternative algorithms designed for a pre-specified small seed set size. We develop a novel sketch-based design for influence computation. Our greedy Sketch-based Influence Maximization (SKIM) algorithm scales to graphs with billions of edges, with one to two orders of magnitude speedup over the best greedy methods. It still has a guaranteed approximation ratio, and in practice its quality nearly matches that of exact greedy. We also present influence oracles, which use linear-time preprocessing to generate a small sketch for each node, allowing the influence of any seed set to be quickly answered from the sketches of its nodes.Comment: 10 pages, 5 figures. Appeared at the 23rd Conference on Information and Knowledge Management (CIKM 2014) in Shanghai, Chin

Immediate performance of self-etching versus system adhesives with multiple light-activated restoratives

Objectives: The purpose of this study was to evaluate the performance of both single and double applications of (Adper Prompt L-Pop) self-etching dental adhesive, when used with three classes of light-activated restorative materials, in comparison to the performance of each restorative system adhesive. Evaluation parameters to be considered for the adhesive systems were (a) immediate marginal adaptation (or gap formation) in tooth cavities, (b) free setting shrinkage-strain determined by the immediate marginal gap-width in a non-bonding Teflon cavity, and (c) their immediate shear bond-strengths to enamel and to dentin. Methods: The maximum marginal gap-width and the opposing-width (if any) in the tooth cavities and in the Teflon cavities were measured immediately (3 min) after light-activation. The shear bond-strengths to enamel and to dentin were also measured at 3 min. Results: For light-activated restorative materials during early setting (&#60;3 min), application of Adper Prompt L-Pop exhibited generally superior marginal adaptation to most system adhesives. But there was no additional benefit from double application. The marginal-gaps in tooth cavities and the marginal-gaps in Teflon cavities were highly correlated (r=0.86–0.89, p&#60;0.02–0.01). For enamel and dentin shear bond-strengths, there were no significant differences between single and double applications, for all materials tested except Toughwell and Z 250 with enamel. Significance: Single application of a self-etch adhesive was a feasible and beneficial alternative to system adhesives for several classes of restorative. Marginal gap-widths in tooth cavities correlated more strongly with free shrinkage-strain magnitudes than with bond-strengths to tooth structure.</p