Chitosan extracted from the Persian Gulf chiton shells: Induction of apoptosis in liver cancer cell line

Here for the first time, we investigated the cytotoxic effects of the chitosan extracted from the Persian Gulf Chiton shell (Acanthopleura vaillantii) on liver cancer cell line (HepG_2). Chitosan extraction was implemented following this method: chitin was produced by demineralization and deproteinization procedure, and the extracted chitin was converted into soluble chitosan using deacetylation method. The cytotoxic effects of extracted chitosan were evaluated using four different tests, including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Annexin V-FITC, propidium iodide (PI) staining, 4',6-diamidino-2-phenylindole (DAPI) staining, and Caspase activity analysis. The IC_50 inhibitory concentrations of chitosan were obtained at 250 µg/mL after 24 h. Chitosan clearly inhibited the growth of hepatocarcinoma cells in vitro in a dose-dependent manner. For detecting the induced cell apoptosis, HepG_2 cells were treated with 125, 250 and 500 µg/ml of chitosan for 24 h. According to the result of Annex in V/PI kit, in 125, 250, and 500 µg/ml of chitosan, 28.2, 49.1, and 83.3% of HepG_2 cells undergone late apoptosis, respectively. The morphology of treated cells by DAPI staining showed non uniform plasma membrane and DNA fragmentation compared to untreated cells with perfect nucleus. The analysis of cell cycle using flow cytometry demonstrated that the rate of sub-G1 peak was increased to 52.7%. Both caspase-3 and -9 activities increased by the extracted chitosan, but it was only significant for caspase-3. The results of the present study suggested that the extracted chitosan has efficient cytotoxicity on HepG_2 cells. Therefore, the extracted chitosan from the shell of the Chiton may be considered as a futuristic natural product regarding the treatment of liver cancer

Electron beam structuring of Ti6Al4V: New insights on the metal surface properties influencing the bacterial adhesion

Soft tissue adhesion and infection prevention are currently challenging for dental transmucosal or percutaneous orthopedic implants. It has previously been shown that aligned micro-grooves obtained by Electron Beam (EB) can drive fibroblast alignment for improved soft tissue adhesion. In this work, evidence is presented that the same technique can also be effective for a reduction of the infection risk. Grooves 10-30 \u3bcm wide and around 0.2 \u3bcm deep were obtained on Ti6Al4V by EB. EB treatment changes the crystalline structure and microstructure in a surface layer that is thicker than the groove depth. Unexpectedly, a significant bacterial reduction was observed. The surfaces were characterized by field emission scanning electron microscopy, X-ray diffraction, confocal microscopy, contact profilometry, wettability and bacterial adhesion tests. The influence of surface topography, microstructure and crystallography on bacterial adhesion was systematically investigated: it was evidenced that the bacterial reduction after EB surface treatment is not correlated with the grooves, but with the microstructure induced by the EB treatment, with a significant bacterial reduction when the surface microstructure has a high density of grain boundaries. This correlation between microstructure and bacterial adhesion was reported for the first time for Ti alloys

Electrohydrodynamic drying versus conventional drying methods: A comparison of key performance indicators

Preserving fruits and vegetables by drying is a traditional yet effective way of reducing food waste. Existing drying methods are either energy-intensive or lead to a significant reduction in product quality. Electrohydrodynamic (EHD) drying is an energy-efficient low-temperature drying method that presents an opportunity to comply with the current challenges of existing drying methods. However, despite its promising characteristics, EHD drying is yet to be accepted by industry and farmers. The adoption of EHD drying is hindered due to different reasons, such as uncertainties surrounding its scalability, quality of dried product, cost of operation, and sustainability compared to conventional drying methods. To address these concerns, this study quantifies and benchmarks the Key Performance Indicators (KPIs) of EHD drying compared to the standard conventional drying methods based on lab-scale experiments. These drying methods include hot-air, freeze, microwave, and solar drying. The results show that drying food using EHD is at least 1.6, 20, and 70 times more energy-efficient than the microwave, freeze, and hot-air, respectively. Similar results could be observed for exergy efficiency. EHD drying has superior product quality compared to other drying methods. For instance, it could retain 62% higher total phenolic content with 21% less color degradation than freeze-drying. Although microwave drying resulted in significantly higher drying kinetics than other techniques, EHD performed better than solar and freeze-drying but was comparable with hot-air drying. EHD drying also shows promising results in economic performance assessment. It is the cheapest drying method after solar drying and has the highest estimated net present value (NPV) after hot-air drying. Overall, compared to the currently used drying methods for small to medium-scale drying, EHD was found to be a more exergy and energy-efficient, cost-effective, and sustainable alternative that can provide higher-quality dried products. However, its drying kinetics should be improved for industrial applications

Effects of Pyriproxyfen on Life Table Indices of Plutella xylostella

The diamondback moth (DBM), Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a major pest of Brassicaceae family in Iran. This study investigated the sublethal effects of pyriproxyfen, a juvenile hormone analogue, on DBM, for two subsequent generations. The leaf dip bioassay method was adopted in conducting this experiment. Sublethal concentrations resulted in a significant decrease in fecundity, oviposition period, and pupal weight of parents and offspring generations. Also, the development time of DBM was prolonged after exposure to pyriproxyfen. The biological parameters such as net reproductive rate (R0) and intrinsic (rm) and finite (λ) rate of increase were lower than control in treatment groups, significantly. From our investigations, pyriproxyfen is a good choice for control of the diamondback moth population through continuous generations

A study on relationship between female employees’ organizational citizenship behavior and job satisfaction within organization

This paper presents an investigation to assess the relationship between female employees’ organizational citizenship behavior and job satisfaction within organization. A questionnaire is designed and distributed among employees who work in city of Qom, Iran and after analyzing the responses by SPSS software program, the relationships between variables are assessed by Pearson test after confirming the normality of the data using Kolmogrov-Smirnov test. The results of the studies reflect a strong relationship between citizen-organization behavior of female employees and job satisfaction. In addition, the survey examines six sub-hypotheses and confirms that there are positive and meaningful relationships between female employees’ custom, altruism, work consciousness, mutual coordination, fairness and courtesy on one side and job satisfaction on the other side

Effect of coagulation and sonication on the dissolved air flotation (DAF) process for thickening of biological sludge in wastewater treatment

Background: Dissolved air floatation (DAF) is one of the methods has been used for the sludge thickening in wastewater treatment plants. This study aimed to investigate the effects of coagulation and sonication processes as additional configurations on the efficiency of a lab-scale DAF process for thickening of the biological sludge of an industrial wastewater treatment plant in Kashan, Iran. Methods: The required amounts of sludge samples were collected from a wastewater treatment plant and kept at temperature of 4°C. Variables, such as pressure (3, 5, and 7 atm), flotation time (5 and 10 minutes), ultrasonic irradiation power (0, 75, and 150 W), and presence/absence of Fe-based coagulant were considered on a sequencing batch reactor (SBR) included coagulation, flotation, and sonication processes, respectively. Results: The use of ultrasonic waves led to an insignificant increase in the DAF efficiency (P > 0.05), however, the application of coagulant significantly increased the thickening efficiency (P < 0.05). The maximum efficiency of the process was achieved at flotation time of 5 min, pressure of 3 atm, and sonication power of 75 W. Conclusion: According to the results, DAF has a proper efficiency for thickening of biological sludge. Coagulation compared to sonication has a greater effect on the efficiency of the process. Keywords: Ultrasonic waves, Industrial effluent, Dissolved air flotation, Sonicatio

Surveying and evaluation services quality in container terminals (case study: Shahid Rajaee container terminal)

This research aims to evaluate quality services in the Rajaee Port container terminal by using SERVQUAL model that is a tool for measuring quality services, identification and for analyzing available gaps between expectations and perceptions services. Container terminal customers at the port of Rajaee are community statistics in this Research. Judgmental sampling method use for selecting sample. The standard and localized questionnaires with Cronbach's alpha coefficient in the perceptions section was 0.921 and in expectations section was 0.895 that was distributed among the 150 members of sample, which 102 (68%) questionnaires were available. First for analyze the data, the researcher used of LISREL software to check reliability SERVQUAL model (Size Making Model First and Second term) and then used SPSS software to do T Test pair sample for analyzing significant gap between expectations and perceptions respondents. Results obtained in this study indicated that there is a gap between customer’s expectation services and perceptions services in container terminal of Rajaee Port in all five dimension of quality services and from weight point of view. the physical dimension is the most important dimension between all dimensions and after that, reliability, assurance, responsiveness and empathy are also important. In addition, physical dimension have maximum gap and empathy dimension have minimum gap between customers’ expectations and perceptions

Vision-based particle filtering for quad-copter attitude estimation using multirate delayed measurements

In this paper, the problem of attitude estimation of a quad-copter system equipped with a multi-rate camera and gyroscope sensors is addressed through extension of a sampling importance re-sampling (SIR) particle filter (PF). Attitude measurement sensors, such as cameras, usually suffer from a slow sampling rate and processing time delay compared to inertial sensors, such as gyroscopes. A discretized attitude kinematics in Euler angles is employed where the gyroscope noisy measurements are considered the model input, leading to a stochastic uncertain system model. Then, a multi-rate delayed PF is proposed so that when no camera measurement is available, the sampling part is performed only. In this case, the delayed camera measurements are used for weight computation and re-sampling. Finally, the efficiency of the proposed method is demonstrated through both numerical simulation and experimental work on the DJI Tello quad-copter system. The images captured by the camera are processed using the ORB feature extraction method and the homography method in Python-OpenCV, which is used to calculate the rotation matrix from the Tello’s image frames