Complete Genome Sequence of Bifidobacterium longum W11 (LMG P-21586), Used as a Probiotic Strain

We report the complete genome sequence of Bifidobacterium longum W11 (LMG P-21586) isolated from the intestinal microbiota of a healthy man. The analysis of the sequence may provide insights into the microbiological characteristics and the functional activity of this probiotic strain

The role of bifidobacteria in predictive and preventive medicine: A focus on eczema and hypercholesterolemia

Bifidobacteria colonize the human gastrointestinal tract early on in life, their interaction with the host starting soon after birth. The health benefits are strain specific and could be due to the produced polysaccharides. The consumption of probiotics may prevent obesity, irritable bowel syndrome, eczema or atopic dermatitis, and asthma. Non-replicative strains of Bifidobacterium longum (NCC3001 and NCC2705) promote the differentiation of normal human epidermal keratinocytes (NHEKs), inducing a high expression of differentiation markers (keratin —KRT1—, and transglu-taminase —TGM1—) and pro-regeneration markers (cathepsins), including β-defensin-1, which plays an important role in modulating the cutaneous immune response. Strains belonging to the genera Bifidobacterium and Lactobacillus can increase tight-junction proteins in NHEKs and enhance barrier function. Bifidobacteria and lactobacilli may be used as prophylactic or therapeutic agents towards enteric pathogens, antibiotic-associated diarrhea, lactose intolerance, ulcerative colitis, irritable bowel syndrome, colorectal cancer, cholesterol reduction, and control of obesity and metabolic disorders. Bifidobacterium bifidum showed an in vitro capability of lowering cholesterol levels thanks to its absorption into the bacterial membrane. Several strains of the species Lactobacillus acidophilus, L. delbrueckii subsp. bulgaricus, L. casei, and L. gasseri led to a reduced amount of serum cholesterol due to their ability to assimilate cholesterol (in vitro). Lactococcus lactis KF147 and Lactobacillus plantarum Lp81 have also been shown to reduce cholesterol levels by 12%. Clarifying the specific health mechanisms of Bifidobacterium and Lactobacillus strains in preventing high-cost pathologies could be useful for delineating effective guidelines for the treatment of infants and adults

The "15-minutes station": a case study to evaluate the pedestrian accessibility of railway transport in Southern Italy

The recent sustainability challenges that our world is facing have raised, more than ever, the attention to the mobility of passengers and freight in the European and international agendas. The energy transition that has begun globally requires identifying and adopting safe, resilient, and increasingly sustainable mobility solutions. In this perspective, the modal split of passengers plays an essential role. One of the main encouraged policies is to promote an efficient mass rapid transit in urban and suburban areas. More in detail, when considering rail transport, it is necessary to analyze and evaluate the role of stations from at least two points of view: i) the ease of access to the station; ii) the opportunities that can be easily reached in its surroundings, following the concept of the "15-minutes" city. These two issues should be properly addressed to guarantee the role of railway stations as an access point to the transport system and an infrastructural element that can enhance a territory. Starting from these considerations, this research proposes a GIS-based methodology able to analyse railway stations from two points of view: i) walkability, considering the main functional characteristics of the transport network, and ii) impact on the territory, by identifying the services located in an area corresponding to "15-minutes" distances using active modes. For each railway station, the main activities in a 15-minute walking isochrone can be evaluated, both considering the walking distance on the pedestrian network and taking into account the current walkability of each link based on arc characteristics. This allows to study the accessibility of railway stations based on the current pedestrian network and the potential one with ideal characteristics. The method is applied to a case study located in Sicily (Italy), in the case of some urban stations. The final scope is to design a decision-support framework useful for railway station operators and local decision-makers to support strategic decisions regarding the railway system and the planning of appropriate pedestrian transport networks to increase railway station accessibility