1,409 research outputs found

    Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae, contains a RecQ helicase-like gene.

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    In most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18?26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses showed that TAH1 homologues are present in all other anomalous M. anisopliae strains studied, as well as in some other polymorphic strains, where the recQ-like gene also appears to be telomere-associated.Published online: 2 June 2005

    Analytical relationship for the cranking inertia

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    The wave function of a spheroidal harmonic oscillator without spin-orbit interaction is expressed in terms of associated Laguerre and Hermite polynomials. The pairing gap and Fermi energy are found by solving the BCS system of two equations. Analytical relationships for the matrix elements of inertia are obtained function of the main quantum numbers and potential derivative. They may be used to test complex computer codes one should develop in a realistic approach of the fission dynamics. The results given for the 240^{240}Pu nucleus are compared with a hydrodynamical model. The importance of taking into account the correction term due to the variation of the occupation number is stressed.Comment: 12 pages, 4 figure

    Wideband digital phase comparator for high current shunts

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    A wideband phase comparator for precise measurements of phase difference of high current shunts has been developed at INRIM. The two-input digital phase detector is realized with a precision wideband digitizer connected through a pair of symmetric active guarded transformers to the outputs of the shunts under comparison. Data are first acquired asynchronously, and then transferred from on-board memory to host memory. Because of the large amount of data collected the filtering process and the analysis algorithms are performed outside the acquisition routine. Most of the systematic errors can be compensated by a proper inversion procedure. The system is suitable for comparing shunts in a wide range of currents, from several hundred of milliampere up to 100 A, and frequencies ranging between 500 Hz and 100 kHz. Expanded uncertainty (k=2) less than 0.05 mrad, for frequency up to 100 kHz, is obtained in the measurement of the phase difference of a group of 10 A shunts, provided by some European NMIs, using a digitizer with sampling frequency up to 1 MHz. An enhanced version of the phase comparator employs a new digital phase detector with higher sampling frequency and vertical resolution. This permits to decrease the contribution to the uncertainty budget of the phase detector of a factor two from 20 kHz to 100 kHz. Theories and experiments show that the phase difference between two high precision wideband digitizers, coupled as phase detector, depends on multiple factors derived from both analog and digital imprint of each sampling system.Comment: 20 pages, 9 figure

    Spatially Resolved Far-Ultraviolet Spectroscopy of the Nuclear Region of NGC 1068

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    We carry out high-resolution FUSE spectroscopy of the nuclear region of NGC 1068. The first set of spectra was obtained with a 30" square aperture that collects all emission from the narrow-line region. The data reveal a strong broad OVI component of FWHM ~3500 kms-1 and two narrow OVI 1031/1037 components of ~350 kms-1. The CIII 977 and NIII 991 emission lines in this spectrum can be fitted with a narrow component of FWHM ~1000 kms-1 and a broad one of ~2500 kms-1. Another set of seven spatially resolved spectra were made using a long slit of 1.25" X 20", at steps of ~1" along the axis of the emission-line cone. We find that (1) Major emission lines in the FUSE wavelength range consist of a broad and a narrow component; (2) There is a gradient in the velocity field for the narrow OVI component of ~200 kms-1 from ~2" southwest of the nucleus to ~4" northeast. A similar pattern is also observed with the broad OVI component, with a gradient of ~3000 kms-1. These are consistent with the HST/STIS findings and suggest a biconical structure in which the velocity field is mainly radial outflow; (3) A major portion of the CIII and NIII line flux is produced in the compact core. They are therefore not effective temperature diagnostics for the conical region; and (4) The best-fitted UV continuum suggests virtually no reddening, and the HeII 1085/1640 ratio suggests a consistently low extinction factor across the cone.Comment: To appear in the Astrophysical Journal. 37 pages with 12 figure

    HST Observations of the Serendipitous X-ray Companion to Mrk 273: Cluster at z=0.46?

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    We have used HST I-band images to identify Mrk 273X, the very unusual high-redshift X-ray-luminous Seyfert 2 galaxy found by ROSAT in the same field-of-view as Mrk 273. We have measured the photometric properties of Mrk 273X and have also analyzed the luminosity distribution of the faint galaxy population seen in the HST image. The luminosity of the galaxy and the properties of the surrounding environment suggest that Mrk 273X is the brightest galaxy in a relatively poor cluster at a redshift near 0.46. Its off-center location in the cluster and the presence of other galaxy groupings in the HST image may indicate that this is a dynamically young cluster on the verge of merging with its neighboring clusters. We find that Mrk 273X is a bright featureless elliptical galaxy with no evidence for a disk. It follows the de Vaucouleurs (r^{1/4}) surface brightness law very well over a range of 8 magnitudes. Though the surface brightness profile does not appear to be dominated by the AGN, the galaxy has very blue colors that do appear to be produced by the AGN. Mrk 273X is most similar to the IC 5063 class of active galaxies --- a hybrid Sy 2 / powerful radio galaxy.Comment: Accepted for publication in the Astrophysical Journal. 8 pages, including 4 postscript figures. Uses emulateapj.sty and psfig.sty. Higher quality version of Figure 1 is available at http://rings.gsfc.nasa.gov/~borne/fig1-markgals.gi

    Time-odd mean fields in the rotating frame: microscopic nature of nuclear magnetism

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    The microscopic role of nuclear magnetism in rotating frame is investigated for the first time in the framework of the cranked relativistic mean field theory. It is shown that nuclear magnetism modifies the expectation values of single-particle spin, orbital and total angular momenta along the rotational axis effectively creating additional angular momentum. This effect leads to the increase of kinematic and dynamic moments of inertia at given rotational frequency and has an impact on effective alignments.Comment: 16 pages, 4 figures, submitted to Physical Review

    Generalized Involution Models for Wreath Products

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    We prove that if a finite group HH has a generalized involution model, as defined by Bump and Ginzburg, then the wreath product H≀SnH \wr S_n also has a generalized involution model. This extends the work of Baddeley concerning involution models for wreath products. As an application, we construct a Gelfand model for wreath products of the form A≀SnA \wr S_n with AA abelian, and give an alternate proof of a recent result due to Adin, Postnikov, and Roichman describing a particularly elegant Gelfand model for the wreath product \ZZ_r \wr S_n. We conclude by discussing some notable properties of this representation and its decomposition into irreducible constituents, proving a conjecture of Adin, Roichman, and Postnikov's.Comment: 29 page

    Detection of Trichomonas vaginalis Using the Polymerase Chain Reaction in Pregnant and Non-Pregnant Women

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    Objective: Trichomonas vaginalis vaginal infections are often both asymptomatic and difficult to detect by current methods. We evaluated the ability of a newly developed polymerase chain reaction (PCR) assay to identify T. vaginalis in vaginal samples from pregnant and non-pregnant women

    Investigating on the nuclear obscuration in two types of Seyfert 2 galaxies

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    We build a large sample of Seyfert 2 galaxies (Sy2s) with both optical spectropolarimetric and X-ray data available, in which 29 Sy2s with the detection of polarized broad emission line (PBL) and 25 without. We find that for luminous Sy2s with L_[OIII] > 10^41 erg/s, sources with PBL have smaller X-ray absorption column density comparing with those without PBL (at 92.3% confidence level): most of the Sy2s with N_H<10^23.8 cm^-2 show PBL (86%, 12 out 14), while the fraction is much smaller for sources with heavier obscuration (54%, 15 out 28). The confidence level of the difference in absorption bounces up to 99.1% while using the "T" ratio (F_2-10keV/F_[O III]) as an indicator. We rule out observation or selection bias as the origin for the difference. Our results, for the first time with high statistical confidence, show that, in additional to the nuclei activity, the nuclear obscuration also plays an important role in the visibility of PBL in Sy2s. These results can be interpreted in the framework of the unified model. We can reach these results in the unified model if: a) the absorption column density is higher at large inclinations and b) the scattering region is obscured at large inclinations.Comment: 28 pages, including 7 figures and 3 tables, ApJ accepte

    A Radio Study of the Seyfert Galaxy IC 5063: Evidence for Fast Gas Outflow

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    New radio continuum (8 GHz and 1.4 GHz) and HI 21 cm line observations of the Seyfert 2 galaxy IC 5063 (PKS 2048-572) were obtained with the Australia Telescope Compact Array (ATCA). The 8 GHz image reveals a linear triple structure (~4'', 1.5 kpc) oriented perpendicular to the optical polarization position angle. It is aligned with the inner dust lane and shows strong morphological association with the narrow emission line region (NLR). At 21 cm, very broad (~700 km/s) HI absorption is observed against the strong continuum source. This absorption is almost entirely blueshifted, indicating a fast net outflow, but a faint and narrow redshifted component is also present. In IC 5063 we see clear evidence for strong shocks resulting from the radio plasma-ISM interaction in the central few kpc. However, the energy flux in the radio plasma is an order of magnitude smaller than the energy emitted in emission lines. Thus, shocks are unlikely to account solely for the global ionization of the emission line region, particularly at large distances. The HI emission outlines a warped disk associated with the system of dust lanes some ~2' (~38 kpc) in radius. The lack of kinematically disturbed gas outside the central few kpc, coupled with the disk warp and close morphological connection of the inner dust lanes and the large-scale ionized gas, support the idea that the gas at large radii is photoionized by the central region, while shadowing effects are important in defining its X-shaped morphology. The kinematics of the ionized and of the neutral gas suggests the existence of a dark halo.Comment: 18 pages, 8 Postscript figures, 3 jpeg figures, Postscript preprint is available from http://jhufos.pha.jhu.edu/~zlatan/papers.htm
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