Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae, contains a RecQ helicase-like gene.

In most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18?26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses showed that TAH1 homologues are present in all other anomalous M. anisopliae strains studied, as well as in some other polymorphic strains, where the recQ-like gene also appears to be telomere-associated.Published online: 2 June 2005

The influence of Harmonia axyridis morbidity on 2-Isopropyl-3-methoxypyrazine in 'Cabernet Sauvignon' wine

Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae; the Multicolored Asian Lady Beetle; MALB) is a vineyard pest in many winemaking regions of the world due to its ability to taint juice and wine (‘ladybug taint’) through excretion or extraction of 2-isopropyl-3-methoxypyrazine (IPMP) when the beetles are incorporated with the grapes at harvest. A common vineyard intervention is the use of insecticidal sprays and the resulting dead beetles are often incorporated in with the harvested fruit. The main objective of this study was to quantify the impact of dead MALB on IPMP concentrations in red wine. Duplicate 'Cabernet Sauvignon' wines were produced with the addition of 10 beetles·l-1 juice, added either live or at 1, 3, 7, or 60-days postmortem. A control wine with no added beetles was included. IPMP concentration was substantially higher in live-beetle wines, and decreased to base-line levels at approximately 6.5 days post-mortem. These results should assist in decisions on viticultural interventions, such as timing of insecticidal sprays, as well as the establishment of grape quality parameters.

The Mid-Pliocene sea-level conundrum: Glacial isostasy, eustasy and dynamic topography

Determining eustatic sea level during the Mid-Pliocene warm period (~ 3.3 to 2.9 Ma) has been a central but elusive goal in the study of past warm climates. Estimates of eustatic sea level based on geologic data span a broad range; variation that we now recognize is due in part to geographically varying post-depositional displacement caused by glacial isostatic adjustment and dynamic topography. In this study, we combine field observations and glacial isostatic adjustment modeling to estimate the dynamic topography signal in three areas that are important to paleo-sea level studies of the Mid-Pliocene warm period (South Africa, West Australia and southeastern United States). We show that dynamic topography played a significant role in the post-depositional displacement of Pliocene, and even younger Pleistocene, shorelines. In this regard, we provide a robust paleo-sea level elevation data set, corrected for glacial isostatic adjustment, that can be used to evaluate predictions from mantle flow models of dynamic topography

Effects of crack tip geometry on dislocation emission and cleavage: A possible path to enhanced ductility

We present a systematic study of the effect of crack blunting on subsequent crack propagation and dislocation emission. We show that the stress intensity factor required to propagate the crack is increased as the crack is blunted by up to thirteen atomic layers, but only by a relatively modest amount for a crack with a sharp 60$^\circ$ corner. The effect of the blunting is far less than would be expected from a smoothly blunted crack; the sharp corners preserve the stress concentration, reducing the effect of the blunting. However, for some material parameters blunting changes the preferred deformation mode from brittle cleavage to dislocation emission. In such materials, the absorption of preexisting dislocations by the crack tip can cause the crack tip to be locally arrested, causing a significant increase in the microscopic toughness of the crack tip. Continuum plasticity models have shown that even a moderate increase in the microscopic toughness can lead to an increase in the macroscopic fracture toughness of the material by several orders of magnitude. We thus propose an atomic-scale mechanism at the crack tip, that ultimately may lead to a high fracture toughness in some materials where a sharp crack would seem to be able to propagate in a brittle manner. Results for blunt cracks loaded in mode II are also presented.Comment: 12 pages, REVTeX using epsfig.sty. 13 PostScript figures. Final version to appear in Phys. Rev. B. Main changes: Discussion slightly shortened, one figure remove

HST Observations of the Serendipitous X-ray Companion to Mrk 273: Cluster at z=0.46?

We have used HST I-band images to identify Mrk 273X, the very unusual high-redshift X-ray-luminous Seyfert 2 galaxy found by ROSAT in the same field-of-view as Mrk 273. We have measured the photometric properties of Mrk 273X and have also analyzed the luminosity distribution of the faint galaxy population seen in the HST image. The luminosity of the galaxy and the properties of the surrounding environment suggest that Mrk 273X is the brightest galaxy in a relatively poor cluster at a redshift near 0.46. Its off-center location in the cluster and the presence of other galaxy groupings in the HST image may indicate that this is a dynamically young cluster on the verge of merging with its neighboring clusters. We find that Mrk 273X is a bright featureless elliptical galaxy with no evidence for a disk. It follows the de Vaucouleurs (r^{1/4}) surface brightness law very well over a range of 8 magnitudes. Though the surface brightness profile does not appear to be dominated by the AGN, the galaxy has very blue colors that do appear to be produced by the AGN. Mrk 273X is most similar to the IC 5063 class of active galaxies --- a hybrid Sy 2 / powerful radio galaxy.Comment: Accepted for publication in the Astrophysical Journal. 8 pages, including 4 postscript figures. Uses emulateapj.sty and psfig.sty. Higher quality version of Figure 1 is available at http://rings.gsfc.nasa.gov/~borne/fig1-markgals.gi

Detection of Trichomonas vaginalis Using the Polymerase Chain Reaction in Pregnant and Non-Pregnant Women

Objective: Trichomonas vaginalis vaginal infections are often both asymptomatic and difficult to detect by current methods. We evaluated the ability of a newly developed polymerase chain reaction (PCR) assay to identify T. vaginalis in vaginal samples from pregnant and non-pregnant women

Hydrological and associated biogeochemical consequences of rapid global warming during the Paleocene-Eocene Thermal Maximum

The Paleocene-Eocene Thermal Maximum (PETM) hyperthermal, ~ 56 million years ago (Ma), is the most dramatic example of abrupt Cenozoic global warming. During the PETM surface temperatures increased between 5 and 9 °C and the onset likely took < 20 kyr. The PETM provides a case study of the impacts of rapid global warming on the Earth system, including both hydrological and associated biogeochemical feedbacks, and proxy data from the PETM can provide constraints on changes in warm climate hydrology simulated by general circulation models (GCMs). In this paper, we provide a critical review of biological and geochemical signatures interpreted as direct or indirect indicators of hydrological change at the PETM, explore the importance of adopting multi-proxy approaches, and present a preliminary model-data comparison. Hydrological records complement those of temperature and indicate that the climatic response at the PETM was complex, with significant regional and temporal variability. This is further illustrated by the biogeochemical consequences of inferred changes in hydrology and, in fact, changes in precipitation and the biogeochemical consequences are often conflated in geochemical signatures. There is also strong evidence in many regions for changes in the episodic and/or intra-annual distribution of precipitation that has not widely been considered when comparing proxy data to GCM output. Crucially, GCM simulations indicate that the response of the hydrological cycle to the PETM was heterogeneous – some regions are associated with increased precipitation – evaporation (P – E), whilst others are characterised by a decrease. Interestingly, the majority of proxy data come from the regions where GCMs predict an increase in PETM precipitation. We propose that comparison of hydrological proxies to GCM output can be an important test of model skill, but this will be enhanced by further data from regions of model-simulated aridity and simulation of extreme precipitation events

A Radio Study of the Seyfert Galaxy IC 5063: Evidence for Fast Gas Outflow

New radio continuum (8 GHz and 1.4 GHz) and HI 21 cm line observations of the Seyfert 2 galaxy IC 5063 (PKS 2048-572) were obtained with the Australia Telescope Compact Array (ATCA). The 8 GHz image reveals a linear triple structure (~4'', 1.5 kpc) oriented perpendicular to the optical polarization position angle. It is aligned with the inner dust lane and shows strong morphological association with the narrow emission line region (NLR). At 21 cm, very broad (~700 km/s) HI absorption is observed against the strong continuum source. This absorption is almost entirely blueshifted, indicating a fast net outflow, but a faint and narrow redshifted component is also present. In IC 5063 we see clear evidence for strong shocks resulting from the radio plasma-ISM interaction in the central few kpc. However, the energy flux in the radio plasma is an order of magnitude smaller than the energy emitted in emission lines. Thus, shocks are unlikely to account solely for the global ionization of the emission line region, particularly at large distances. The HI emission outlines a warped disk associated with the system of dust lanes some ~2' (~38 kpc) in radius. The lack of kinematically disturbed gas outside the central few kpc, coupled with the disk warp and close morphological connection of the inner dust lanes and the large-scale ionized gas, support the idea that the gas at large radii is photoionized by the central region, while shadowing effects are important in defining its X-shaped morphology. The kinematics of the ionized and of the neutral gas suggests the existence of a dark halo.Comment: 18 pages, 8 Postscript figures, 3 jpeg figures, Postscript preprint is available from http://jhufos.pha.jhu.edu/~zlatan/papers.htm

Generalized Involution Models for Wreath Products

We prove that if a finite group $H$ has a generalized involution model, as defined by Bump and Ginzburg, then the wreath product $H \wr S_n$ also has a generalized involution model. This extends the work of Baddeley concerning involution models for wreath products. As an application, we construct a Gelfand model for wreath products of the form $A \wr S_n$ with $A$ abelian, and give an alternate proof of a recent result due to Adin, Postnikov, and Roichman describing a particularly elegant Gelfand model for the wreath product \ZZ_r \wr S_n. We conclude by discussing some notable properties of this representation and its decomposition into irreducible constituents, proving a conjecture of Adin, Roichman, and Postnikov's.Comment: 29 page