THE RELATIONSHIP OF VACUUM CHILDBIRTH WITH THE INCIDENCE OF ASPHYXIA NEONATORUM

Asphyxia neonatorum is a condition that needs attention because it is related to the quality of life so that at every delivery it is hoped that there will be no disabilities that can affect life in the future. The purpose of this study was to determine the relationship between the relationship of vacuum childbirth with the incidence of asphyxia neonatorum in RSUD Dr. M. Yunus Bengkulu. This research was a quantitative study with a cross sectional design. The total sample was 115 patients that who experienced asphyxia at birth by vacuum extraction. The results showed there was a significant correlation between vacuum childbirth with the incidence of asphyxia neonatorum in RSUD Dr. M. Yunus Bengkulu with p value <0.05

Comparison of a Novel Whole Blood Transglutaminase-based ELISA With a Whole Blood Rapid Antibody Test and Established Conventional Serological Celiac Disease Assays

Objectives: Serum immunoglobulin A-class tissue transglutaminase (tTG-ab) and endomysial antibody (EMA) tests play a key tests play a key role in the diagnostic evaluation of celiac disease. Recently, a novel whole blood rapid test based on self-tissue transglutaminase (tTG) was developed for celiac disease case finding. Based on the same principle, a whole blood self-tTG enzyme-linked immunosorbent assay (ELISA), especially applicable to large-scale screening of celiac disease, has been produced. We assessed the value of this new test in celiac disease antibody detection. Patients and Methods: The new test uses endogenous tTG found in red blood cells of whole blood in IgA-class tTG-ab measurement by detecting tTG-tTG-ab complexes formed after hemolysis of the whole e blood sample. Stored whole blood samples from 150 untreated celiac disease patients and 107 control individuals without celiac disease Were evaluated, and the test results were compared with those of the whole blood rapid test, 2 conventional serum-based tTG-ab ELISA tests, and 2 EMA tests. Results: A total of 15 whole blood samples were found to tic clotted or dried after storage and were excluded from further evaluation. The whole blood ELISA test had a specificity (98%) comparable to that of the conventional serological tests, the sensitivity (91%) being slightly lower. The test was concordant with the whole blood rapid test in 92% of cases, with 2 serological ELISA tests in 91% and 94% of cases and with EMA tests in 94% and 93% of cases. Conclusions: Whole blood self-tTG-based testing is accurate in celiac antibody detection, also when all ELISA method is applied. The testing requires no serum separation or external tTG