Lipidomic profiling of plasma and erythrocytes from septic patients reveals potential biomarker candidates

FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOSepsis remains the primary cause of death from infection, despite advances in modern medicine. The identification of reliable diagnostic biomarkers for the early detection of this disease is critical and may reduce the mortality rate as it could allow ear13113FAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOsem informação162191/2015-4The authors thank FAPESP, for financial support, the colleagues, and students of the Unidade Integrada de Farmacologia e Gastroenterologia (UNIFAG) and Laboratório de Análises Clínicas São José from Hospital Universitário São Francisco na Providência de

Analytical methods applied to diverse types of Brazilian propolis

Propolis is a bee product, composed mainly of plant resins and beeswax, therefore its chemical composition varies due to the geographic and plant origins of these resins, as well as the species of bee. Brazil is an important supplier of propolis on the world market and, although green colored propolis from the southeast is the most known and studied, several other types of propolis from Apis mellifera and native stingless bees (also called cerumen) can be found. Propolis is usually consumed as an extract, so the type of solvent and extractive procedures employed further affect its composition. Methods used for the extraction; analysis the percentage of resins, wax and insoluble material in crude propolis; determination of phenolic, flavonoid, amino acid and heavy metal contents are reviewed herein. Different chromatographic methods applied to the separation, identification and quantification of Brazilian propolis components and their relative strengths are discussed; as well as direct insertion mass spectrometry fingerprinting

Investigation of the anti-inflammatory and analgesic activities of a sample of Brazilian propolis

El Propóleo es ampliamente usado en Brasil en la medicina popular en las enfermedades inflamatorias. Ese trabajo estudió la composición química y las actividades antiinflamatoria y antinociceptiva del extracto hidroalcohólico de propóleo (EHP) de Atibaia, São Paulo, Brazil. Los resultados de los análisis químicos revelaron el predominio del ácido 3,5-diprenil-4-hidroxinámico y sus derivados y de los ácidos p-cumárico, cafeico y derivados. La actividad anti-inflamatoria del EHP per os fue evaluada en el edema de pata inducido por la carragenina en ratas y edema de oreja de ratón inducido por aceite de croton. En dosis de 250 mg.Kg-1 causó una reducción significativa en el edema de la pata en dos horas (51,36%) después de inyección subplantar de carragenina en las ratas, persistiendo el efecto hasta la tercera hora (29,68%). Las dosis de 500 y 1000 mg.Kg-1 promovieron la reducción del edema del 52,1% y 60,3% en la primera hora, del 65,6% y 59,7% a la segunda hora, del 56,1% y 52,8% a la tercera hora y del 41,4% y 36,9% en la cuarta hora, respectivamente. Los efectos del EHP administrados en el edema de la oreja inducido por aceite de croton en los ratones en las dosis de 250, 500 y 1000 mg.Kg-1 mostraron reducción del edema en 47%, 50,5% y 48,1%, respectivamente. Para evaluar la actividad antinociceptiva del EHP, administrado oralmente, fueron usados los dos modelos experimentales ("writhing test" en los ratones y "tail-flick" en las ratas). Los resultados obtenidos en el "writhing test" con EHP solamente en la dosis de 1000 mg.Kg-1 significativamente inhibieron las contorsiones en 52,8%. En el análisis "tail-flick", los resultados obtenidos con la administración por vía oral de EHP no evidenciaron actividades analgésicas en ninguna de las dosis utilizadas, pero en administración de 1000 mg.Kg-1, ip, demostraron significativa actividad antinociceptiva, incrementando la latencia de retirada de la cola de las ratas, valor comparable a la droga control morfina. La administración de naloxone asociada al EHP o morfina, ip, disminuyó la latencia de la retirada de las colas de las ratas, respectivamente, en los tiempos 9,67 y 4,9 s; estos resultados sugieren que el EHP contiene sustancias antinociceptivas que parecen estar relacionadas con la activación de los receptores opioides.Propolis is extensively used in Brazilian folk medicine for inflammatory diseases. This work studied the chemical composition, anti-inflammatory and antinociceptive activities of a hydro alcoholic extract of propolis (HEP) from Atibaia, São Paulo, Brazil. The results of the analyses reveal a predominance of 3,5-diprenyl-4-hydroxicinamic acid, and derivatives, as well as p-coumaric acid, caffeic acid its derivatives. The anti-inflammatory activity of HEP, per os was evaluated in the carrageenin-induced rat paw oedema model and mouse ear oedema induced by croton oil. At doses of 250 mg.Kg-1, HEP caused a significant reduction in paw oedema two hours (51.36%) after the subplantar injection of carrageenin in rats, persisting until the third hour (29.68%). Doses of 500 and 1000 mg.Kg-1, promoted the reduction of edema from the first hour until the fourth; after the first hour 52.1% and 60.3%; after two hours 65.6% and 59.7%; after three hours 56.1% and 52.8% and after four hours 41.4% and 36.9%, respectively. The effects of HEP administered on croton oil-induced ear oedema in mice at doses of 250, 500 and 1000 mg.Kg-1, showed reduction of oedema of 47%, 50,5% and 48,1%, respectively. To evaluate the antinociceptive activity of HEP by oral administration, two experimental models were used (writhing test in mice and tail-flick in rats). The results obtained in the writhing test showed that HEP at a dose of 1000 mg.Kg-1 significantly inhibited the acetic acid-induced writhing in mice, decreasing the contortions in 52,8%. In the tail-flick assay, the results obtained with the oral administration of HEP did not show evidence of analgesic activities at the doses used, but an ip administration of 1000 mg.Kg-1, demonstrated signficative antinociceptive activity increasing the latency of tail retreat in rats, comparable to the control drug, morphine. The ip administration of naloxone associated with HPE or morphine, decreased the latency of tail retreat in rats, such results suggest that HEP contain antinociceptive substances which appear to be unrelated to the activation of opioid receptors.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Investigation of the anti-inflammatory and analgesic activities of a sample of Brazilian propolis

El Propóleo es ampliamente usado en Brasil en la medicina popular en las enfermedades inflamatorias. Ese trabajo estudió la composición química y las actividades antiinflamatoria y antinociceptiva del extracto hidroalcohólico de propóleo (EHP) de Atibaia, São Paulo, Brazil. Los resultados de los análisis químicos revelaron el predominio del ácido 3,5-diprenil-4-hidroxinámico y sus derivados y de los ácidos p-cumárico, cafeico y derivados. La actividad anti-inflamatoria del EHP per os fue evaluada en el edema de pata inducido por la carragenina en ratas y edema de oreja de ratón inducido por aceite de croton. En dosis de 250 mg.Kg-1 causó una reducción significativa en el edema de la pata en dos horas (51,36%) después de inyección subplantar de carragenina en las ratas, persistiendo el efecto hasta la tercera hora (29,68%). Las dosis de 500 y 1000 mg.Kg-1 promovieron la reducción del edema del 52,1% y 60,3% en la primera hora, del 65,6% y 59,7% a la segunda hora, del 56,1% y 52,8% a la tercera hora y del 41,4% y 36,9% en la cuarta hora, respectivamente. Los efectos del EHP administrados en el edema de la oreja inducido por aceite de croton en los ratones en las dosis de 250, 500 y 1000 mg.Kg-1 mostraron reducción del edema en 47%, 50,5% y 48,1%, respectivamente. Para evaluar la actividad antinociceptiva del EHP, administrado oralmente, fueron usados los dos modelos experimentales ("writhing test" en los ratones y "tail-flick" en las ratas). Los resultados obtenidos en el "writhing test" con EHP solamente en la dosis de 1000 mg.Kg-1 significativamente inhibieron las contorsiones en 52,8%. En el análisis "tail-flick", los resultados obtenidos con la administración por vía oral de EHP no evidenciaron actividades analgésicas en ninguna de las dosis utilizadas, pero en administración de 1000 mg.Kg-1, ip, demostraron significativa actividad antinociceptiva, incrementando la latencia de retirada de la cola de las ratas, valor comparable a la droga control morfina. La administración de naloxone asociada al EHP o morfina, ip, disminuyó la latencia de la retirada de las colas de las ratas, respectivamente, en los tiempos 9,67 y 4,9 s; estos resultados sugieren que el EHP contiene sustancias antinociceptivas que parecen estar relacionadas con la activación de los receptores opioides.Propolis is extensively used in Brazilian folk medicine for inflammatory diseases. This work studied the chemical composition, anti-inflammatory and antinociceptive activities of a hydro alcoholic extract of propolis (HEP) from Atibaia, São Paulo, Brazil. The results of the analyses reveal a predominance of 3,5-diprenyl-4-hydroxicinamic acid, and derivatives, as well as p-coumaric acid, caffeic acid its derivatives. The anti-inflammatory activity of HEP, per os was evaluated in the carrageenin-induced rat paw oedema model and mouse ear oedema induced by croton oil. At doses of 250 mg.Kg-1, HEP caused a significant reduction in paw oedema two hours (51.36%) after the subplantar injection of carrageenin in rats, persisting until the third hour (29.68%). Doses of 500 and 1000 mg.Kg-1, promoted the reduction of edema from the first hour until the fourth; after the first hour 52.1% and 60.3%; after two hours 65.6% and 59.7%; after three hours 56.1% and 52.8% and after four hours 41.4% and 36.9%, respectively. The effects of HEP administered on croton oil-induced ear oedema in mice at doses of 250, 500 and 1000 mg.Kg-1, showed reduction of oedema of 47%, 50,5% and 48,1%, respectively. To evaluate the antinociceptive activity of HEP by oral administration, two experimental models were used (writhing test in mice and tail-flick in rats). The results obtained in the writhing test showed that HEP at a dose of 1000 mg.Kg-1 significantly inhibited the acetic acid-induced writhing in mice, decreasing the contortions in 52,8%. In the tail-flick assay, the results obtained with the oral administration of HEP did not show evidence of analgesic activities at the doses used, but an ip administration of 1000 mg.Kg-1, demonstrated signficative antinociceptive activity increasing the latency of tail retreat in rats, comparable to the control drug, morphine. The ip administration of naloxone associated with HPE or morphine, decreased the latency of tail retreat in rats, such results suggest that HEP contain antinociceptive substances which appear to be unrelated to the activation of opioid receptors.Colegio de Farmacéuticos de la Provincia de Buenos Aire

The impacts of the raising regime of salmon species on their triacylglycerol composition revealed by easy ambient sonic-spray ionization mass spectrometry

Marine fish consumption is rising around the world due to the high quality of its nutritional components, including long chain polyunsaturated fatty acids (LC-PUFA), which are abundant and found as triacylglycerol (TAG) in the muscle and skin of Salmonidae family. Farm raised and wild species of Salmon have different diets, that directly influences their TAG composition. In this work, we demonstrate the evaluation of TAG composition of salmon species as an authenticity screening parameter. For this purpose, we propose the use of ambient mass spectrometry, here represented by the thermal imprinting (TI) easy ambient sonic-spray ionization mass spectrometry (EASI-MS), to offer a fast and ecofriendly method for TAG extraction and characterization of fish muscles and skins. Specifically, TI-EASI-MS was employed to obtain the TAG profiles of different species from Salmonids, which were compared using multivariate statistical analysis. Clear differentiation of wild-raised fishes was achieved based on their higher content of ions attributed to TAG containing PUFA when compared to farm-raised fishes. The TI extraction method was also compared to the standard Bligh & Dyer extraction protocol and the techniques were equivalent for Salmon species differentiation1201925CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPnão temnão temnão te

Processo De Identificação, Caracterização E Determinação Da Falsificação De Méis Naturais Provenientes De Abelhas Apis Melifera E Nativas

PROCESSO DE IDENTIFICAÇÃO, CARACTERIZAÇÃO E DETERMINAÇÃO DA FALSIFICAÇÃO DE MÉIS NATURAIS PROVENIENTES DE ABELHAS APIS MELIFERA E NATIVAS. A presente invenção descreve um processo destinado a diferenciação de méis de abelhas Apis melifera ou nativas, ambas de diferentes floradas, e para determinação de falsificação dos mesmos, pela adição de xarope de glicose, caramelo, açúcar invertido e óleos vegetais, através de marcadores químicos com as técnicas de ionização por dessorção a laser assistida por matriz e de ionização a pressão atmosférica acopladas a espectrometria de massas.BRPI0802501 (A2)G01N30/68A23L1/08G01N30/72G01N33/02BR2008PI02501G01N30/68A23L1/08G01N30/72G01N33/0

Electrospray ionization mass spectrometry fingerprinting of propolis of native Brazilian stingless bees

Stingless bees are found in many tropical and subtropical regions of the word. The knowledge of the composition of their propolis as well as the plants that are visited as sources of resins is therefore of prime importance. Here the negative ion mode electrospray ionization mass spectrometry [ESI(-)-MS] fingerprints of propolis from various species of native stingless bees from different regions in Brazil are compared to determine their composition patterns. The correlation among the propolis samples was investigated via chemometric analysis

Antiproliferative activity, antioxidant capacity and chemical composition of extracts from the leaves and stem of Chresta sphaerocephala

In this study, antiproliferative and antioxidant activities of crude extracts (hexane, ethyl acetate and methanol) from leaves and stem of Chresta sphaerocephala DC., Asteraceae, were investigated. Antiproliferative activity was tested in vitro against ten human cancer cells and against VERO (no cancer cell). Antioxidant activities were determined using DPPH and ORAC-FL assays and the total phenolic content was estimated by Folin–Ciocalteu method. Hexane and ethyl acetate extracts (leaves and stem) exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 50.40 and 250 µg/ml. For VERO cell, TGI values were >250 µg/ml for all extracts, except to hexane extract of the stem (TGI 80.92 µg/ml). In an initial evaluation, ethyl acetate and methanol extracts (leaves and stem) have shown levels of phenolic compounds between 6.94 and 30.96 mg GAE/kg in Folin–Ciocalteu assay, DPPH free-radical scavenging activity with SC50 in the range of 75.22 and 400 µg/ml and antioxidant capacity between 290.08 and 1088 µmol TE/g of extract in ORAC-FL assay. HPLC-DAD and ESI-MS analysis allowed the identification of flavonoids in the methanol extract from the leaves of C. sphaerocephala. Three steroids and nine triterpenoids were identified in the bioactive hexane extracts using HRGC.254369374COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informaçãoSem informaçã

Antiproliferative activity, antioxidant capacity and chemical composition of extracts from the leaves and stem of Chresta sphaerocephala

In this study, antiproliferative and antioxidant activities of crude extracts (hexane, ethyl acetate and methanol) from leaves and stem of Chresta sphaerocephala DC., Asteraceae, were investigated. Antiproliferative activity was tested in vitro against ten human cancer cells and against VERO (no cancer cell). Antioxidant activities were determined using DPPH and ORAC-FL assays and the total phenolic content was estimated by Folin–Ciocalteu method. Hexane and ethyl acetate extracts (leaves and stem) exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 50.40 and 250 μg/ml. For VERO cell, TGI values were >250 μg/ml for all extracts, except to hexane extract of the stem (TGI 80.92 μg/ml). In an initial evaluation, ethyl acetate and methanol extracts (leaves and stem) have shown levels of phenolic compounds between 6.94 and 30.96 mg GAE/kg in Folin–Ciocalteu assay, DPPH free-radical scavenging activity with SC50 in the range of 75.22 and 400 μg/ml and antioxidant capacity between 290.08 and 1088 μmol TE/g of extract in ORAC-FL assay. HPLC-DAD and ESI-MS analysis allowed the identification of flavonoids in the methanol extract from the leaves of C. sphaerocephala. Three steroids and nine triterpenoids were identified in the bioactive hexane extracts using HRGC. Keywords: Chresta sphaerocephala, Steroids, Terpenoids, Flavonoids, Antiproliferative and antioxidant activitie