Kinetics of invertase synthesis by Saccharomyces cerevisiae in synthetic medium

Present investigation deals with optimization of appropriate substrate concentration and incubation temperature both for growth of Saccharomyces cerevisiae and invertase production. Submerged fermentation technique was employed in the present study. The maximal production of invertase during the course of study was achieved after 48 h of incubation using initial sucrose concentration, 15.0 g l-1. The sugar consumption and dry cell mass were also examined. Data was subjected to kinetic analysis and on the basis of kinetic parameters such as Yp/x (amount of enzyme produced mg-1 cell mass), Yp/s (amount of enzyme produced mg-1 sugar consumed), Yx/s (mg cells mg-1 substrate consumed), Ys/x (mg sugar consumed mg-1 cell mass produced), qp (Amount of enzyme produced mg-1 sugar consumed h-1), qs (mg mg-1 cells h-1), qx (mg cells mg-1 sugar consumed h-1), µ (mg cells produced h-1), it was found that temperature had a direct influence both on substrate consumption and synthesis of enzyme. Similarly, higher concentrations of sucrose in fermentation medium induced catabolite repression of yeast invertase

Revitalising the Planning Commission

This paper considers the key problems confronting the Pakistan Planning Commission, briefly examines the essentials of planning for a mixed economy, draws lessons from the experience of countries, both developed and developing, that have formulated and implemented successful plans, and suggests measures to revitalize the Planning Commission. Some suggestions are put forward for continuous upgrading of the institution’s skills in the longer run. The paper also describes ways in which policymakers can learn from their counterparts in successful countries in the region

PENDEKATAN COLLABORATIVE GOVERNANCE DALAM PENGELOLAAN SAMPAH PADA BANK SAMPAH KECAMATAN MANGGALA

Garbage is a complex problem that every big city has. The waste problem is acommon problem that requires efforts and efforts from the community, privatesector actors and the government to handle it. Therefore, one of the efforts of the Makassar City Government in handling waste is the establishment of a WasteBank in each region with a Central Waste Bank under the Makassar CityEnvironmental Service. Collaborative Governance was born due to limitedresources and government finances in the continuity of the management of theWaste Bank which is now in every District of Makassar City. One of them is theManggala Sector Waste Bank under the leadership of the Manggala DistrictGovernment which is now collaborating with PT. Pegadaian since 2019. Theinscription of cooperation between Manggala District and PT. Pegadaian wasinaugurated in 2019 and inaugurated by the deputy mayor of Makassar with thetheme “The Gade Clean & Gold Waste Sorting Program Saving Gold PT.Pawnshops and Manggala District". In the ongoing collaboration process,indicators are met in building communication which is reflected by weighingwaste involving community participation, building commitments that have thehopes and desires of the collaborating parties to play a role in sorting waste sothat the volume of waste entering the landfill can be controlled, building trustfrom parties involved. collaborating is the main supporter in achievingconsistency in collaborative performance, and understanding in achievingresults includes the creation of a clean environment and public understandingthat waste can be of value. The research was conducted using qualitativemethods, namely interviews and field observations

Integrating Science and Art of Medicine

The art of medicine and science of medicine are not antagonistic but supplementary to each other. There are both important differences and similarities between science and arts . Art rediscovers , generation after generation , what is necessary to humanness . Basic elements of art, according to Aristotle , are symmetry, proportion and an organic order of parts in united whole. True art is moral. It seeks to improve life and not to debase it. Even artistic modes needs scientific evaluation .Art does not mean wandering in the wilderness. By enlarge medical professionals try to use and construct all these artistic needs in the human body and soul. It is naïve to say that art does badly what science does well, or the contrary. Illness, disease, and medical context provide the mixture of emotion, drama, irony, humour, blood and gore which is grist to the mill of the novel, play, film and a TV serial. Medicine and art have a common goal to complete what nature cannot bring to a finish, i.e., to reach the ideal, to heal creation. This is done by paying attention. Physician attends the patient, artist attends the nature. Art like medicine is not an arrival. It’s a search. That is why medicine by itself is called an art. Being a doctor, considered as a species of logic and rationality, need not to be frightened by the art, as if it is some thing superfluous. Oscar Wilde said very rightly “Life imitates art far more than the art imitates life” &nbsp

INCREASED LOW CARBON STEEL HARDNESS BY CARBURIZING METHOD

INCREASED LOW CARBON STEEL HARDNESS BY CARBURIZING METHO

ANALISIS PENENTUAN FAKTOR PREDIKTOR TINGKAT SKALA STIGMA EMIC (EXPLANATORY MODEL INTERVIEW CATALOGUE) TERMODIFIKASI TERHADAP KUSTA PADA MASYARAKAT GAMPONG MEUNASAH KEUDEE KECAMATAN MASJID RAYA ACEH BESAR

Penyakit kusta merupakan salah satu jenis penyakit menular yang masih menjadi masalah kesehatan di Indonesia. Selain itu, kusta juga dapat menimbulkan masalah sosial yang menyebabkan diskriminasi pada penderita kusta oleh stigma masyarakat terhadap kusta. Penelitian ini bertujuan untuk mengetahui tinggi rendahya stigma terhadap kusta pada masyarakat menggunakan Skala EMIC yang berstandar Internasional. Desain penelitian ini adalah analitik dengan metode cross sectional dan jumlah responden sebanyak 85 orang Penelitian ini dilakukan di bulan November 2013 pada masyarakat Gampong Meunasah Keudee Aceh Besar. Analisis data diuji menggunakan chi-square dan analisis regresi logistik. Hasil penelitian didapatkan faktor yang mempengaruhi tingkat stigma pada masyarakat Gampong Meunasah Keudee adalah ketakutan, pendidikan, dan pengetahuan dengan p value < 0,05, dimana ketakutan adalah hal yang paling mempengaruhi tinggi rendahnya stigma terhadap kusta pada masyarakat Gampong Meunasah Keudee Aceh Besar dan tingkat rata-rata stigma pada masyarakat Gampong Meunasah Keudee adalah tinggi dengan nilai EMIC > 21 , sedangkan faktor jenis kelamin, status perkawinan, pendapatan dan umur tidak mempengaruhi tingkat skala stigma EMIC dengan p value > 0,05. Kesimpulan: Faktor yang mempengaruhi stigma terhadap kusta pada masyarakat adalah ketakutan, pengetahuan, dan pendidikan. Kata Kunci: EMIC, stigma, kust