1,181 research outputs found
Comparative genomics and mutagenesis analyses of choline metabolism in the marine Roseobacter clade
Choline is ubiquitous in marine eukaryotes and appears to be widely distributed in surface marine waters; however, its metabolism by marine bacteria is poorly understood. Here, using comparative genomics and molecular genetic approaches, we reveal that the capacity for choline catabolism is widespread in marine heterotrophs of the marine Roseobacter clade (MRC). Using the model bacterium Ruegeria pomeroyi, we confirm that the betA, betB and betC genes, encoding choline dehydrogenase, betaine aldehyde dehydrogenase and choline sulfatase, respectively, are involved in choline metabolism. The betT gene, encoding an organic solute transporter, was essential for the rapid uptake of choline but not glycine betaine (GBT). Growth of choline and GBT as a sole carbon source resulted in the re-mineralization of these nitrogen-rich compounds into ammonium. Oxidation of the methyl groups from choline requires formyltetrahydrofolate synthetase encoded by fhs in R.pomeroyi, deletion of which resulted in incomplete degradation of GBT. We demonstrate that this was due to an imbalance in the supply of reducing equivalents required for choline catabolism, which can be alleviated by the addition of formate. Together, our results demonstrate that choline metabolism is ubiquitous in the MRC and reveal the role of Fhs in methyl group oxidation in R.pomeroyi
Resonance distribution in open quantum chaotic systems
In order to study the resonance spectra of chaotic cavities subject to some
damping (which can be due to absorption or partial reflection at the
boundaries), we use a model of damped quantum maps. In the high-frequency
limit, the distribution of (quantum) decay rates is shown to cluster near a
``typical'' value, which is larger than the classical decay rate of the
corresponding damped ray dynamics. The speed of this clustering may be quite
slow, which could explain why it has not been detected in previous numerical
data.Comment: 4 pages. Compared with version 2, we have slightly modified the
figures, corrected some misprints, and added the values for the fits in
figure
On the determination of Moving Boundaries for Hyperbolic Equations
We consider wave equations in domains with time-dependent boundaries (moving
obstacles) contained in a fixed cylinder for all time. We give sufficient
conditions for the determination of the moving boundary from the Cauchy data on
part of the boundary of the cylinder. We also study the related problem of
accessibility of the moving boundary by time-like curves from the boundary of
the cylinder.Comment: The proof of Theorem 4.1 is expanded, Example 1 of section 4.3 is
improved, misprints are correcte
Variational formulas of higher order mean curvatures
In this paper, we establish the first variational formula and its
Euler-Lagrange equation for the total -th mean curvature functional
of a submanifold in a general Riemannian manifold
for . As an example, we prove that closed
complex submanifolds in complex projective spaces are critical points of the
functional , called relatively -minimal submanifolds,
for all . At last, we discuss the relations between relatively -minimal
submanifolds and austere submanifolds in real space forms, as well as a special
variational problem.Comment: 13 pages, to appear in SCIENCE CHINA Mathematics 201
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