26 research outputs found
Expression in E. coli of finger-domain lacking tissue-type plasminogen activator with high fibrin affinity
AbstractTissue-type plasminogen activator (t-PA) has a high affinity for fibrin and induces lysis of fibrin (fibrinolysis) on the surface of fibrin without degrading circulating fibrinogen. cDNA for t-PA which lacks the ‘fingerdomain’ (the site for fibrin affinity) was isolated from Detroit 562 cells. Analysis of the nucleotide sequence revealed a lack of the sequences which code for the finger-domain. A plasmid (pDPAT 1) containing the Escherichia coli tac promoter/operator and the cDNA sequence coding for ‘finger-domain lacking t-PA’ was constructed for expression in E. Coli. The polypeptide so produced was a new type of t-PA lacking finger-domain, but revealed plasminogen activator activity with the function of fibrin affinity.Tissue-type plasminogen activatorFinger-domainFibrin affinityPlasmid E. coli tac promoter/operatorDetroit 562 cel
Estudo comparativo, prospectivo e randomizado entre uroterapia e tratamento farmacológico em crianças com incontinência urinária
OBJECTIVE: To verify and compare the results of behavioral modification plus pelvic floor muscle training and behavioral modifications plus oxybutynin chloride in children with nonmonosymptomatic enuresis. METHODS: A total of 47 children were randomized using opaque and sealed envelopes sequentially numbered. Group I was composed of 21 children who underwent antimuscarinic treatment (oxybutynin), and Group II was composed of 26 patients who underwent pelvic floor muscle training. Both groups were instructed as to behavioral modifications. RESULTS: The voiding diary results were compared each month between Groups I and II. In the first month of treatment, children in Group I presented 12.2 dry nights, 13.4 in the second month, and 15.9 in the last month. In Group II, the results were: 14.9 dry nights in the first month, 20.8 dry nights in the second and 24.0 dry nights in the last month. There was a significant difference between the groups in second and third months. CONCLUSION: Pelvic floor exercises associated with behavioral changes were more effective than pharmacological treatment in children with urinary incontinence.OBJETIVO: Verificar e comparar os resultados da modificação comportamental associado ao treinamento dos músculos do assoalho pélvico e modificação comportamental associado ao uso de cloridrato de oxibutinina em crianças com enurese não monossintomática. MÉTODOS: Foram randomizadas 47 crianças por meio de envelopes opacos e selados com numeração sequencial. O Grupo I foi composto por 21 crianças que receberam tratamento com antimuscarÃnico (oxibutinina) e o Grupo II por 26 pacientes que receberam treinamento dos músculos do assoalho pélvico. Ambos os grupos foram instruÃdos em relação à modificação comportamental. RESULTADOS: Os resultados do diário miccional foram comparados cada mês entre os Grupos I e II. No primeiro mês de tratamento, as crianças do Grupo I apresentaram 12,2 noites secas, 13,4 no segundo mês e 15,9 no último mês. No Grupo II, os resultados foram: 14,9 noites secas no primeiro mês, 20,8 no segundo mês e 24,0 no último mês. Houve diferença significativa entre os grupos no segundo e no terceiro mês. CONCLUSÃO: Os exercÃcios do assoalho pélvico associados a mudança comportamental foram mais efetivos do que o tratamento farmacológico em crianças com incontinência urinária.20320
Subject, protagonist and agent/patient
Osamu Ikari. Subject, protagonist and agent/patient. In: Cahiers de linguistique - Asie orientale, vol. 10, 1981. pp. 43-66
Tratamento percutaneo dos calculos vesicais
Orientador: Nelson Rodrigues Netto JrDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências MédicasResumo: No perÃodo de outubro de 1984 a agosto de 1994, quarenta e dois pacientes, portadores de cálculo vesical, foram tratados por via percutânea. Obteve-se sucesso em trinta e oito pacientes ( 90% ), e o insucesso em quatro pacientes (10%) deveu-se a não fragmentação do cálculo pelo ultra-som. Os pacientes foram distribuÃdos em três grupos, conforme a presença de doenças associadas. Dois grupos foram tratados concomitantemente. No grupo I foi realizada cistolitotripsia associada à ressecção transuretral da próstata e no grupo 11, cistolitotripsia juntamente com uretrotomia endoscópica. Num terceiro grupo, o 111, foi empregada apenas a cistolitotripsia. Não foram observadas complicações adicionais, mesmo em pacientes com doenças associadas e não houve diferença significativa, quando os grupos foram comparados (p < 0,55). Para a realização deste procedimento, não há necessidade de tluoroscopia. A via percutânea evita traumas uretrais e representa uma alternativa no tratamento dos cálculos vesicaisAbstract: A total of 42 patients with bladder stones underwent percutaneous suprapubic cystolithotripsy. The success rate was 90%. There were 10% failures due to non ftagmentation of the stones by the ultrasound probe. According to the presence of associated diseases, 3 groups of patients were stablished. Two groups underwent concomitant treatments for benign prostatic hyperplasia and urethral stricture. No complications occurred even in patients with concomitant treatment. There was no statistically significant difference when these groups were compared (p < 0.55). Fluoroscopy was not necessary during that procedure. Since the technique is simple, safe and effective, it represents an alternative in the management ofbladder stonesMestradoCirurgia GeralMestre em Medicin
Behavior of a minute amount of crotonaldehyde in distillation of aqueous ethanol solution under reduced pressure
Intravesical Calculus Secondary To Ethanol Gel Injection Into The Prostate.
A 78-year-old man with coagulopathy and lower urinary tract symptoms secondary to benign prostatic hyperplasia underwent transurethral prostate ethanol gel injection. Six months after the injection, the patient presented with irritative symptoms and hematuria. Ultrasonography revealed a 5-cm intravesical calculus. Cystolitholapaxy was unsuccessful, and the patient underwent cystolithotomy with retrieval of a large, soft, round, brownish mass. Histologic examination of the mass demonstrated prostatic hyperplastic glandular stroma with extensive coagulate necrosis and peripheral calcification. To our knowledge, this is the first report of a calculus formed by prostatic tissue slosh after ethanol injection.65100