Terminal restriction fragment length polymorphism is an “old school” reliable technique for swift microbial community screening in anaerobic digestion

The microbial community in anaerobic digestion has been analysed through microbial fingerprinting techniques, such as terminal restriction fragment length polymorphism (TRFLP), for decades. In the last decade, high-throughput 16S rRNA gene amplicon sequencing has replaced these techniques, but the time-consuming and complex nature of high-throughput techniques is a potential bottleneck for full-scale anaerobic digestion application, when monitoring community dynamics. Here, the bacterial and archaeal TRFLP profiles were compared with 16S rRNA gene amplicon profiles (Illumina platform) of 25 full-scale anaerobic digestion plants. The α-diversity analysis revealed a higher richness based on Illumina data, compared with the TRFLP data. This coincided with a clear difference in community organisation, Pareto distribution, and co-occurrence network statistics, i.e., betweenness centrality and normalised degree. The β-diversity analysis showed a similar clustering profile for the Illumina, bacterial TRFLP and archaeal TRFLP data, based on different distance measures and independent of phylogenetic identification, with pH and temperature as the two key operational parameters determining microbial community composition. The combined knowledge of temporal dynamics and projected clustering in the β-diversity profile, based on the TRFLP data, distinctly showed that TRFLP is a reliable technique for swift microbial community dynamics screening in full-scale anaerobic digestion plants

Metagenomic sequencing unravels gene fragments with phylogenetic signatures of O2-tolerant NiFe membrane-bound hydrogenases in lacustrine sediment

Many promising hydrogen technologies utilising hydrogenase enzymes have been slowed by the fact that most hydrogenases are extremely sensitive to O2. Within the group 1 membrane-bound NiFe hydrogenase, naturally occurring tolerant enzymes do exist, and O2 tolerance has been largely attributed to changes in iron–sulphur clusters coordinated by different numbers of cysteine residues in the enzyme’s small subunit. Indeed, previous work has provided a robust phylogenetic signature of O2 tolerance [1], which when combined with new sequencing technologies makes bio prospecting in nature a far more viable endeavour. However, making sense of such a vast diversity is still challenging and could be simplified if known species with O2-tolerant enzymes were annotated with information on metabolism and natural environments. Here, we utilised a bioinformatics approach to compare O2-tolerant and sensitive membrane-bound NiFe hydrogenases from 177 bacterial species with fully sequenced genomes for differences in their taxonomy, O2 requirements, and natural environment. Following this, we interrogated a metagenome from lacustrine surface sediment for novel hydrogenases via high-throughput shotgun DNA sequencing using the Illumina™ MiSeq platform. We found 44 new NiFe group 1 membrane-bound hydrogenase sequence fragments, five of which segregated with the tolerant group on the phylogenetic tree of the enzyme’s small subunit, and four with the large subunit, indicating de novo O2-tolerant protein sequences that could help engineer more efficient hydrogenases

Malaysian Halal Standard (Ms1500:2004) : Do Halal Food Manufacturers Have Intention To Be Certified ?

The Malaysian government has a vision of becoming a global halal hub. In line with this MS1500:2004 Halal Standard was introduced as extension to the existing halal logo and certification. Kerajaan Malaysia mempunyai visi untuk menjadi hub halal global. Selaras dengan ini, Standard Halal MS1500:2004 diperkenalkan sebagai tambahan kepada pensijilan logo halal yang sedia ada

A New Weighting Scheme in Weighted Markov Model for Predicting the Probability of Drought Episodes

Drought is a complex stochastic natural hazard caused by prolonged shortage of rainfall. Several environmental factors are involved in determining drought classes at the specific monitoring station. Therefore, efficient sequence processing techniques are required to explore and predict the periodic information about the various episodes of drought classes. In this study, we proposed a new weighting scheme to predict the probability of various drought classes under Weighted Markov Chain (WMC) model. We provide a standardized scheme of weights for ordinal sequences of drought classifications by normalizing squared weighted Cohen Kappa. Illustrations of the proposed scheme are given by including temporal ordinal data on drought classes determined by the standardized precipitation temperature index (SPTI). Experimental results show that the proposed weighting scheme for WMC model is sufficiently flexible to address actual changes in drought classifications by restructuring the transient behavior of a Markov chain. In summary, this paper proposes a new weighting scheme to improve the accuracy of the WMC, specifically in the field of hydrology

Compensating for source depth change and observing surface waves using underwater communication signals

Underwater communications impose great challenges due to the unpredictable changes in the environment. In order to accommodate for these changes, equalizers are used to track the Impulse Responses (IRs) and compensate the intersymbol interference (ISI). Source and array depth shifts are one of the major contributing factors to continuous amplitude and phase changes in IR. These changes magnify the problem of data processing in which correlation between successive received signals is involved, e.g. passive Time Reversal (pTR) where a probe signal is sent ahead of the data for post cross-correlation. In this paper, an environment based algorithm is used for pTR equalization, where an appropriate frequency shift of the estimated IR compensates for the geometric changes such as source and array depth shifts. We have applied this Frequency Shift pTR equalizer (FSpTR) on real data collected from Underwater Acoustic Barrier 2007 (UAB’07) sea trial having 1000 baud BPSK signaling at carrier frequency of 6.25 KHz with a sudden source depth change of 0.5 m at various known instants of time. The results illustrate that a considerable gain can be attained using the environment based equalizer in the presence of source and/or array depth changes. Moreover by close analysis of the FSpTR results we have detected surface wave motion through the frequency shifts caused by the array depth variations

Arrival-based equalizer for underwater communication systems

One of the challenges in the present underwater acoustic communication systems is to combat the underwater channel effects which results in time and frequency spreading of the transmitted signal. The time spreading is caused by the multipath effect while the frequency spreading is due to the time variability of the underwater channel. The passive Time Reversal (pTR) equalizer has been used in underwater communications because of its time focusing property which minimizes the time spreading effect of the underwater channel. In order to compensate for the frequency spreading effect, an improved version of pTR was proposed, called Frequency shift passive time reversal (FSpTR). FSpTR tries to compensate for the frequency spreading by applying a frequency shift in the estimated channel impulse response (IR). In the multipath environment, multiple replicas of the transmitted signal reaches the receiver through different paths where each path is affected differently by environmental variations. In such cases, a single frequency shift fails to compensate for the environmental variations on each path, resulting in degradation in the performance. In this paper, an arrival-based equalizer is proposed to compensate for the environmental variations on each path. The concept of beamforming is integrated with FSpTR equalizer, in this paper, to compensate each arrival separately for the environmental variations. The proposed equalizer is tested with the real data and the results showed that the proposed approach outperforms pTR and FSpTR equalizers and provides a mean MSE gain of 4.9 dB and 4.2 dB respectively.This work is supported by Portuguese Foundation for Science Technology under PHITOM (PTDC/EEATEL/71263/2006) and COGNAT (PTDC/MAR/112446/2009) projects. This work was also supported by European Community’s Sixth Framework Program through the grant to the budget of the Integrated Infrastructure Initiative HYDRALAB III within the Transnational Access Activities, Contract no. 022441

Perception of Individual Consumers toward Islamic Banking Products and Services in Pakistan

Islamic banking system was established in Pakistan since 1980. Now, the Islamic banking products are available to serve the customers but these products are not fully accepted by the customers due to the lack of marketing efforts by the providers. To identify the perception about Islamic banking system researcher fill the questionnaires from respondents that what they think about Islamic banking system. And, these respondents are the customers who hold bank account in conventional or Islamic bank. We analyze data through descriptive analysis to find the results about perception of Islamic banking. This study explores the perception of persons that responsible in financial affairs and this study were usage of Islamic banking facilities and respondents understanding of Islamic banking system on different aspects and to selection criteria of bank. Keywords: Consumer Perception, Islamic Banking system, Consumer awareness, features of Islamic ban

Potential of vertical line array beamforming in underwater acoustic communications

Um dos principais tópicos atuais de pesquisa é ́ a concepçãoo de técnicas de processamento de sinal eficazes para comunicações submarinas. Este interesse é reforç ̧ado pelos desafios inerentes às condições incontroláveis, como a batimetria, a temperatura, e as variações temporais (geométricas) das profundidades da fonte e da antena de receptores. A obtenção de taxas de transmissão modestas é ainda um desafio, devido a estas variações. O presente trabalho aborda o uso potencial do formador de feixes (beamformer) no sistema de comunicações submarinas. O propósito de usar um beamformer neste trabalho é o de separar os diferentes caminhos que chegam do transmissor para o recetor, o que é motivado pelo fato de que, num ambiente real, não é possível distinguir todos os caminhos de propagação, no domínio do tempo. Neste trabalho, é usado o beamformer de atraso-e-soma, que aplica atrasos diferentes para cada hidrofone da antena vertical, e adiciona as saídas resultantes. Cada um dos atrasos é função do ângulo de chegada e da profundidade do hidrofone. O objetivo do beamformer é aplicar os atrasos opostos a cada elemento da antena vertical, para garantir a soma coerente da frente de onda numa direção particular. Ambos resultados com dados simulados e dados reais são apresentados neste trabalho, para mostrar o desempenho do beamformer. Os resultados do beamformer são comparados com os resultados da análise Doppler, para caracterizar chegadas diferentes e fazer corresponder essas chegadas às variações Doppler, mostrando que cada chegada é afetada pelas variações ambientais de uma maneira diferente, resultando numa quantidade diferente de Doppler