General non-specific metabolic, neuroendocrine and immune reactions to various water-salt loads in female rats

Background. This article begins with a series of articles on the effects on parameters of water-salt, nitrous and lipid metabolism, as well as the neuroendocrine-immune complex of mineral water, extracted from the bore located in the city Gertsa (Bukovyna, Ukraine). The chemical analysis prompted us to use waters Sophiya and Naftussya from spa Truskavets’ as a reference as well as an artificial salt analogue of Gertsa water, which contains no organic matter or trace elements. Materials and methods. Experiment was performed on 58 healthy female Wistar rats 240-290 g divided into 6 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Instead, the other rats received the same tap water as well as waters Sophiya, Naftussya, Gertsa and its artificial salt analogue through the probe at a dose of 1,5 mL/100 g of body mass for 6 days. The day after the completion of the drinking course in all rats the parameters of metabolism and neuroendocrine-immune complex were registered. Results. Screening registered parameters found 42 among them who in rats subjected to water-salt loads, significantly different from that of intact rats, but on average the same group of animals that received liquids with different mineralization and chemical composition. Conclusion. Takes place nonspecific (general) reaction neuroendocrine-immune complex and metabolism in water-salt load as such, regardless of the specific chemical composition of fluids applied

Role of organic carbon and nitrogen of mineral waters in their metabolic effects at female rats

Background. Organic substances, despite their presence in all drinking mineral waters, are still considered to be active only in the water of Naftussya type (which, due to mineralization less than 1 g/L, are not formally mineral), whereas the physiological activity of true mineral waters (which mineralization is greater than 1-2 g/L) are associated with electrolytes and trace elements. The purpose of this study was to clarify the role of organic carbon and nitrogen of mineral waters in their effects on certain parameters of metabolism. Material and Methods. Experiment was performed on 48 healthy female Wistar rats 240-290 g divided into 5 groups. Animals of the first group for 6 days administered a single tap water through the probe at a dose of 1,5 mL/100 g of body mass. In the second group we administered the water Naftussya from the Truskavets’ layer, in the third group the water Sophiya of the Truskavets’ field. The rats of the fourth group received the native water from the Gertsa (Bucovyna) field, and the last group its artificial salt analogue. The day after the completion of the drinking course in all rats some metabolic parameters were registered. Results. On the basis of the correlation analysis with step-by-step exclusion, four metabolites of blood as well as five metabolites of urine are included in the regressive model for organic carbon (R=0,697; R2=0,486; Adjusted R2=0,347; F(10,4)=3,5; p=0,0025). Organic nitrogen of mineral waters affects five metabolic parameters of urine only, but with approximately the same force (R=0,621; R2=0,385; Adjusted R2=0,312; F(5,4)=5,3; p=0,0008). Conclusion. Organic substances of mineral waters play an essential role in their effects on the parameters of metabolism

Features of neuro-endocrine and immune reactions to various water-salt loads in female rats

Background. Previously, we have shown that the weekly load of rats with water-salt solutions of different chemical compositions causes both general and specific reactions of the parameters of metabolism. The purpose of this study is to identify under these conditions specific neuroendocrine and immune responses. Materials and methods. Experiment was performed on 58 healthy female Wistar rats 240-290 g divided into 6 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Instead, the other rats received the same tap water as well as waters Sophiya, Naftussya, Gertsa and its artificial salt analogue through the probe at a dose of 1,5 mL/100 g of body mass for 6 days. The day after the completion of the drinking course in all rats some neuroendocrine and immune parameters were registered. Results. The method of discriminant analysis revealed 29 parameters of the neuroendocrine-immune complex (10 of them reflect the neuroendocrine regulation, 4 thymus mass and thymocytogram elements, 5 elements of splenocytogram, 10 elements of immunocytogram and leukocytogram of blood and parameters of phagocytosis), according to which the reaction on various water-salt loads are identified with an accuracy of 98.3%. Conclusion. The peculiarities of the reactions of the parameters of the neuroendocrine-immune complex are due to the content of water in sulfate, bicarbonate and magnesium, as well as organic carbon and nitrogen

Similarity Of Effects On EEG Parameters Of Aramaic, Greek Catholic And Krishnaic Prayers

The neurotropic effects of Prayer are manifested in two inhibitory and three activating patterns. The first inhibitory pattern reflects the decrease in elevated and upper limit levels of SPD of θ-and δ-rhythm in frontal loci and the second - decrease in normal SPD levels of β-and θ-rhythm in the frontal, central, temporal and parietal loci. The first activating pattern reflects a small increase in normal levels of β-rhythm index and asymmetry and SPD entropy in locus C3, as well as a further increase in elevated δ-rhythm SPD levels in loci P3 and T3. The second pattern reflects the slight increase in normal SPD levels of θ-rhythm in loci T3, T5, T6, O2 and α-rhythm in locus T5, as well as their indices and entropy SPD in locus O2. The third pattern reflects a slight increase in amplitude and SPD of α-rhythm in central, frontal, temporal and occipital loci

Relationships between caused by Kozyavkin© method changes in parameters of manual function and electroencephalogram, heart rate variability as well as gas discharge visualization in children with spastic form of cerebral palsy

Background. Earlier we reported that in children with spastic forms of cerebral palsy (SFCP) after two-week course of rehabilitation by Kozyavkin© method reducing neural component of muscle tone (NCMT) stated in 79,3% cases while in 13,8% cases changes were not detected and in 2 children it increased. We hypothesized that such a variety of changes in NCMT is due to ambiguous changes in the background activity of the nerve centers. Aim: analysis of relationships between changes (Ch) in NCMT as well as manual functional tests, on the one hand, and parameters of EEG, HRV as well as Gas Discharge Visualization (GDV), on the other hand. Material and research methods. The object of observations were 14 children (6 girls and 8 boys) aged 8÷15 years with SFCP. State motor development at GMFCS was on II÷IV level. Functional status of the hand with MACS was at II÷III level. The estimation of hand function carried out by Dynamometry (D), Box and Block Test (B&B) and Nine Hole Peg Test (NHP). We registered also NCMT by device “NeuroFlexor” (Aggero MedTech AB, Sweden), HRV and EEG simultaneosly by hardware-software complex “Cardiolab+VSR” and “NeuroCom Standard” respectively (KhAI Medica, Kharkiv, Ukraine) as well as GDV by “GDV Chamber” (“Biotechprogress”, St-Pb, RF). Results. After two-week course of rehabilitation at 9 children NCMT reduced from 19,8±3,4 to 12,3±2,8 Newtons (Ch: -7,5±2,0 N), at 3 children NCMT taked 8,2±3,3 before and 7,9±3,5 after rehabilitation (Ch: -0,3±0,3 N) while at one girl NCMT increased from 15,1 to 17,9 N and at one boy from 6,1 to 19,4 N. Manual functional tests also changed ambiguously. The Ch in NCMT are correlated with Ch in parameters HRV&EEG (R2=0,786). The Ch in functional tests of Left hand are correlated with Ch in parameters HRV&EEG to the same extent: the level of R2 is for D 0,799, for NHP 0,773 and for B&B 0,708. Instead, for the Right hand, the correlation is stronger: R2 is 0,973, 0,792 and 0,978 respectively. As regards GDV parameters, connections are weaker, but they are also stronger for the Right hand: R2 is 0,706 vs 0,462 for B&B and 0,679 vs 0,405 for NHP but not for D (0,719 and 0,709). The Ch in NCMT are correlated with Ch in parameters GDV also weaker (R2=0,556). In its turn, changes in GDV parameters are very closely related to changes in parameters of both HRV (R2=0,999) and EEG (R2=0,998). Conclusion. In children with spastic forms of cerebral palsy caused by Kozyavkin© method changes in manual functional tests and neural component of muscle tone are determined by changes in parameters of EEG and HRV as well as GDV

Features of metabolic reactions to various water-salt loads in female rats

Background. In the previous article we reported that screening registered parameters of water-salt, nitrous and lipid metabolism as well as the neuroendocrine-immune complex found 42 among them who in rats subjected to various water-salt loads, significantly different from that of intact rats, but on average the same group of animals that received liquids with different mineralization and chemical composition. The purpose of this article is to find out the features of the reactions of the parameters of metabolism. Materials and methods. Experiment was performed on 58 healthy female Wistar rats 240-290 g divided into 6 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Instead, the other rats received the same tap water as well as waters Sophiya, Naftussya, Gertsa and its artificial salt analogue through the probe at a dose of 1,5 mL/100 g of body mass for 6 days. The day after the completion of the drinking course in all rats the parameters of water-salt, nitrous and lipid metabolism were registered. Results. Found that 16 metabolic parameters the maximum deviates from the level of intact rats under the influence of the salt analogue of Gertsa water, a smaller, but tangible effect is made by the Gertsa native water, even less effective waters Sofiya and Naftussya, instead of ordinary water is almost ineffective in relation to these metabolic parameters. The other 19 parameters deviates to a maximum extent from the reference level after the use of water Naftussya, fresh water is less effective, whereas quasi-isotonic liquids are practically inactive for these parameters. The remaining 13 parameters in animals that use normal water, deviates from intact control to the same extent as in the previous pattern, which, apparently, is also due to the stressful effects of the load course. Both Naftussya and Gertsa water and its salt analogue prevent the stress deviations of these parameters. Instead, by consumption of water Sofiya stresses deviations of these parameters is reversed. The method of discriminant analysis revealed 33 variables (among them 8 refer to plasma/erythrocytes electrolytes, 7 to electrolytes of urine, to other metabolic parameters of plasma 5 and urine 9, as well as glomerular filtration, canalicular reabsorption, diurese and urine osmolarity), the totality of which the metabolic reactions to various water-salt loads are identified (recognized) with an accuracy of 98,3%. Conclusion. The features of the reactions of the parameters of metabolism are due to the content in waters NaCl, SO42- as well as organic carbon and nitrogen