Elastic constants of borocarbides. New approach to acoustic Measurement technique

A new version of the phase method of determining the sound velocity is proposed and implemented. It utilizes the ``Nonius'' measurement technique and can give acceptable accuracy (~1%) in samples of submillimeter size. Measurements of the sound velocity are made in single-crystal samples of the borocarbides RNi2B2C (R = Y,Lu,Ho). The elastic constants and the Debye temperature are calculated.Comment: 5 figures, 2 table

On the leaf development in Oedipodium (Oedipodiales, Bryophyta)

Leaf development in Oedipodium griffithianum was studied based on herbarium and living material, using microscopic observations of plants at different stages of development and series of sections. It turned out that the apical cell may lose its bifacial structure, thus the leaves develop the bilaterally symmetric areolation pattern, similar to that seen in Oedipodium protonematayesBelgorod State National Research Universit

A Minimal Model of Ribosome Allocation Dynamics Captures Trade-offs in Expression between Endogenous and Synthetic Genes

Cells contain a finite set of resources that must be distributed across many processes to ensure survival. Among them, the largest proportion of cellular resources is dedicated to protein translation. Synthetic biology often exploits these resources to execute orthogonal genetic circuits, yet the burden this places on the cell is rarely considered. Here, we develop a minimal model of ribosome allocation dynamics capturing the demands on translation when expressing a synthetic construct together with endogenous genes required for maintenance of cell physiology. Critically, it contains three key variables related to design parameters of the synthetic construct covering: transcript abundance, translation initiation rate, and elongation time. We show that model-predicted changes in ribosome allocation closely match experimental shifts in synthetic protein expression rate and cellular growth. Intriguingly, the model is also able to accurately infer transcript levels and translation times after further exposure to additional ambient stress. Our results demonstrate that a simple model of resource allocation faithfully captures the redistribution of protein synthesis resources when faced with the burden of synthetic gene expression and environmental stress. The tractable nature of the model makes it a versatile tool for exploring the guiding principles of efficient heterologous expression and the indirect interactions that can arise between synthetic circuits and their host chassis due to competition for shared translational resources

Miscellaneous notes on the genus Forsstroemia in Russia (Neckeraceae, Bryophyta)

Molecular phylogenetic analysis supports the position on the species known as Neckera konoi in the genus Forsstroemia, therefore it is transferred to this genus with a new combination, F. konoi (Broth.) Enroth, Fedosov & Ignatov. Molecular data also confirm the position of F. stricta Laz. described from the Russian Far East in F. producta, a pantropical species. Forstroemia neckeroides is reported from Russia, Primorsky Territory, for the first time.Peer reviewe

Original Russian Text ©

In eukaryotes, rRNA genes form multigenic families consisting of tandemly located repeated units strongly varying in number (from several hundreds to several thousands). A repeated unit includes the genes of 18S, 5.8S, and 26S rRNAs separated by transcribed spacers (ITS) 1 and 2 and by the intergenic spacer (IGS). Depending on localization of the 5S rRNA genes, two types of ribosomal operon organization are described In the present work, we studied specific features of the IGS1 structure of the ribosomal operon from acrocarpous mosses of the Schistidium genus for which we studied earlier the ITS1 structure, as well as the phylogeny of the genus based on the ITS1-2 sequences and regions of the chloroplast genome MATERIALS AND METHODS Thirty-three sequences of IGS1 from 12 species of Schistidium were determined. Below the species are listed, ISSN 0006-2979, Biochemistry (Moscow), 2015, Vol. 80, No. 11, pp. 1485-1491. © Pleiades Publishing, Ltd., 2015. Original Russian Text © I. A. Milyutina, E. A. Ignatova, M. S. Ignatov, D. V. Goryunov, A. V. Troitsky, 2015, published in Biokhimiya, 2015, Vol. 80, No. 11, pp. 1707-1714 On-Line Papers in Press, as Manuscript BM15-232, September 27, 2015. 1485 Abbreviations: bp, nucleotide base pair; IGS1, intergenic spacer 1. * To whom correspondence should be addressed. Sciences, 127276 Moscow, Russia; E-mail: [email protected] Received July 8, 2015 Structure of Intergenic Abstract-The structure of the intergenic spacer 1 (IGS1) of the ribosomal operon from 12 species of Schistidium mosses was studied. In the IGS1 sequences of these species, three conserved regions and two areas of GC-and A-enriched repeats were identified. All of the studied mosses have a conserved pyrimidine-enriched motif at the 5′-end of IGS1. Species-specific nucleotide substitutions and insertions were found in the conserved areas. The repeated units contain single nucleotide substitutions that make unique the majority of repeated units. The positions of such repeats in IGS1 are species-specific, but their number can vary within the species and among operons of the same specimen. The comparison of IGS1 sequences from the Schistidium species and from representatives of ten other moss genera revealed the presence of common conserved motifs with similar localization. Presumably, these motifs are elements of termination of the pre-rRNA transcription and processing of rRNA

Thermal structure in the Venus middle cloud layer

Thermal structure measurements obtained by the two Vega balloons show the Venus atmosphere in the middle cloud layer to be near-adiabatic, on the whole; but discrete air masses are present that differ slightly from one another in potential temperature and entropy. The Vega 1 temperatures are 6.5 K warmer than measured by Vega 2 at given pressures. Measurements taken by the Vega 2 lander on descent through these levels agree with the Vega 2 balloon data

Опыт применения Беродуала-Н при хронической обструктивной болезни легких у рабочих на промышленном предприятии

Chronic obstructive pulmonary disease (COPD) is an actual healthcare problem due to its widespread, progressive course and mortality. A great deal of the patients’ treatment takes a symptomatic therapy. Results of outpatient treatment with Berodual-N of patients with COPD stage 1 to 2 in stable condition are shown in the article. A significant improvement in clinical signs, lung function parameters (FEV1) and physical tolerance were noted.Хроническая обструктивная болезнь легких (ХОБЛ) является актуальной проблемой для здравоохранения из-за широкой распространенности, прогрессирующего течения и смертности. Значительное место в лечении больных ХОБЛ занимает симптоматическая терапия. Приведены результаты лечения с использованием ингаляций Беродуала-Н у пациентов с ХОБЛ I—II стадии при стабильном течении на амбулаторном этапе. Отмечено достоверное улучшение клинических симптомов, показателей функции внешнего дыхания (ОФВ1) и повышение толерантности к физической нагрузке