Sustainability Assessment of Two Farming Systems

Objective: Assess the sustainability of two agricultural production systems (native maize and improved maize) by applying the MESMIS methodology Design/methodology/approach: Knowing the social, economic and environmental factors that make up the production systems in the municipality of Jilotepec, State of Mexico, as well as their forms of management, allow us to develop strategies for sustainable rural development. The study contemplated at 30 production systems and compared the level of sustainability between producers of native maize and improved maize. The collection of information in the field was carried out in September 2022 and consisted of participatory workshops, semi-structured interviews and surveys; this information was complemented by soil sampling to analyze fertility Results: The two systems studied have high costs of agricultural production and high dependence on external inputs, especially those using improved maize. Most systems depend on external economic income. Agriculture is increasingly exposed to the conditions of drought and changes in rainfall regimes, in this situation having livestock becomes an important economic support. Changing climate conditions are forcing people to implement adaptability measures. Regionally the soil is fertile and suitable for corn cultivation. The perception of happiness of the inhabitants interviewed is high, they consider that the quality of life is good and therefore do not migrate Findings/conclusions: The present study is the result of the integrated analysis of several methodologies used in the measurement of sustainability indicators

In vitro transcription/translation system: A versatile tool in the search for missing proteins

et al.Approximately 18% of all human genes purported to encode proteins have not been directly evidenced at the protein level, according to the validation criteria established by neXtProt, and are considered to be >missing> proteins. One of the goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to identify as many of these missing proteins as possible in human samples using mass spectrometry-based methods. To further this goal, a consortium of C-HPP teams (chromosomes 5, 10, 16, and 19) has joined forces to devise new strategies to identify missing proteins by use of a cell-free in vitro transcription/translation system (IVTT). The proposed strategy employs LC-MS/MS data-dependent acquisition (DDA) and targeted selective reaction monitoring (SRM) methods to scrutinize low-complexity samples derived from IVTT. The optimized assays are then applied to identify missing proteins in human cells and tissues. We describe the approach and show proof-of-concept results for development of LC-SRM assays for identification of 18 missing proteins. We believe that the IVTT system, when coupled with downstream mass spectrometric identification, can be applied to identify proteins that have eluded more traditional methods of detection.Support from the Cancer Prevention Research Institute of Texas (RML 1122 to C.L.N.) and the University of Texas Medical Branch (C.L.N.) is gratefully acknowledged. Financial support from the Carlos III Health Institute of Spain (ISCIII, FIS PI11/02114/FIS PI14/01538 and FIS PI12/00624), Fondos FEDER (EU) is gratefully acknowledged. The proteomics units at CIC and UCM belong to ProteoRed, PRB2-ISCIII, supported by grant PT13/0001. P.D. and C.D. are supported by JCYL-EDU/346/2013 Ph.D. scholarships.Peer Reviewe

Clavigero. Comunidad de saberes, núm. 25: Ante la crisis alimentaria y ambiental, ¿qué hacemos?

La producción desenfrenada de alimentos ha llevado a que la relación entre la oferta y la demanda no sea equitativa. El crecimiento acelerado de la población mundial ha traído como consecuencia el desarrollo de sistemas productivos extensivos para la producción masiva de alimentos a bajo costo. Mientras se desecha aproximadamente 40% de la producción total de alimentos, al mismo tiempo, 690 millones de personas en el mundo están en condiciones de hambruna. Además, la alta tecnificación de los sistemas productivos también ha provocado que los alimentos sean enriquecidos artificialmente, promoviendo dietas poco nutritivas y causando serios problemas de salud. En este número se exponen algunos retos e iniciativas que se han generado en torno a esta compleja problemática que nos envuelve a todos