Validity of the N\'{e}el-Arrhenius model for highly anisotropic Co_xFe_{3-x}O_4 nanoparticles

We report a systematic study on the structural and magnetic properties of Co_{x}Fe_{3-x}O_{4} magnetic nanoparticles with sizes between $5$ to $25$ nm, prepared by thermal decomposition of Fe(acac)_{3} and Co(acac)_{2}. The large magneto-crystalline anisotropy of the synthesized particles resulted in high blocking temperatures ($42$ K \leqq $T_B$ $\leqq 345$ K for $5 \leqq$ d $\leqq 13$ nm ) and large coercive fields ($H_C \approxeq 1600$ kA/m for $T = 5$ K). The smallest particles ($=5$ nm) revealed the existence of a magnetically hard, spin-disordered surface. The thermal dependence of static and dynamic magnetic properties of the whole series of samples could be explained within the N\'{e}el-Arrhenius relaxation framework without the need of ad-hoc corrections, by including the thermal dependence of the magnetocrystalline anisotropy constant $K_1(T)$ through the empirical Br\"{u}khatov-Kirensky relation. This approach provided $K_1(0)$ values very similar to the bulk material from either static or dynamic magnetic measurements, as well as realistic values for the response times ($\tau_0 \simeq 10^{-10}$ s). Deviations from the bulk anisotropy values found for the smallest particles could be qualitatively explained based on Zener\'{}s relation between $K_1(T)$ and M(T)

Design issues and experimental characterization of a continuously-tuned adaptive CMOS LNA

This paper presents the design implementation and experimental characterization of an adaptive Low Noise Amplifier (LNA) intended for multi-standard Radio Frequency (RF) wireless transceivers. The circuit —fabricated in a 90-nm CMOS technology— is a two-stage inductively degenerated common-source topology that combines PMOS varactors with programmable load to make the operation of the circuit continuously tunable. Practical design issues are analyzed, considering the effect of circuit parasitics associated to the chip package and integrated inductors, capacitors and varactors. Experimental measurements show a continuous tuning of NF and Sparameters within the 1.75-2.23GHz band, featuring NF19.6dB and IIP3> −9.8dBm, with a power dissipation < 23mW from a 1-V supply voltage.Ministerio de Ciencia e Innovación (FEDER) TEC2007-67247-C02-01/MICJunta de Andalucía, Consejo Regional de Innovación, ciencia y empresa TIC-253

Cell bystander effect induced by radiofrequency electromagnetic fields and magnetic nanoparticles

Induced effects by direct exposure to ionizing radiation (IR) are a central issue in many fields like radiation protection, clinic diagnosis and oncological therapies. Direct irradiation at certain doses induce cell death, but similar effects can also occur in cells no directly exposed to IR, a mechanism known as bystander effect. Non-IR (radiofrequency waves) can induce the death of cells loaded with MNPs in a focused oncological therapy known as magnetic hyperthermia. Indirect mechanisms are also able to induce the death of unloaded MNPs cells. Using in vitro cell models, we found that colocalization of the MNPs at the lysosomes and the non-increase of the temperature induces bystander effect under non-IR. Our results provide a landscape in which bystander effects are a more general mechanism, up to now only observed and clinically used in the field of radiotherapy.Comment: 16 pages, 4 figures, submitted to International Journal of Radiation Biolog

Protein adsorption onto Fe3O4 nanoparticles with opposite surface charge and its impact on cell uptake

Nanoparticles (NPs) engineered for biomedical applications are meant to be in contact with protein-rich physiological fluids. These proteins are usually adsorbed onto the NP surface, forming a swaddling layer called protein corona that influences cell internalization. We present a study on protein adsorption onto different magnetic NPs (MNPs) when immersed in cell culture medium, and how these changes affect the cellular uptake. Two colloids with magnetite cores of 25 nm, same hydrodynamic size and opposite surface charge were in situ coated with (a) positive polyethyleneimine (PEI-MNPs) and (b) negative poly(acrylic acid) (PAA-MNPs). After few minutes of incubation in cell culture medium the wrapping of the MNPs by protein adsorption resulted in a 5-fold size increase. After 24 h of incubation large MNP-protein aggregates with hydrodynamic sizes 1500 to 3000 nm (PAA-MNPs and PEI-MNPs respectively) were observed. Each cluster contained an estimated number of magnetic cores between 450 and 1000, indicating the formation of large aggregates with a "plum pudding" structure of MNPs embedded into a protein network of negative surface charge irrespective of the MNP_core charge. We demonstrated that PEI-MNPs are incorporated in much larger amounts than the PAA-MNPs units. Quantitative analysis showed that SH-SY5Y cells can incorporate 100 per cent of the added PEI-MNPs up to about 100 pg per cell, whereas for PAA-MNPs the uptake was less than 50 percent. The final cellular distribution showed also notable differences regarding partial attachment to the cell membrane. These results highlight the need to characterize the final properties of MNPs after protein adsorption in biological media, and demonstrate the impact of these properties on the internalization mechanisms in neural cells.Comment: 32 pages, 10 figure

On the Commutative Equivalence of Context-Free Languages

The problem of the commutative equivalence of context-free and regular languages is studied. In particular conditions ensuring that a context-free language of exponential growth is commutatively equivalent with a regular language are investigated