Measuring Plant Genetic Diversity Using Inter-Simple Sequence Repeats (ISSRS)

Simple sequence repeats (SSRs) have great utility as they are conserved and present in all eukaryotic genomes. Here we report the use of a simple PCR with fluorescently-labelled primers to amplify inter-SSR markers (ISSRs) for diversity assessments. The use of ISSR markers does not rely upon specific genetic sequence information, or prolonged method development and may be measured rapidly using the automated equipment. The major restriction of the ISSR method is at the analysis stage, as the markers are dominant it is not possible to distinguish heterozygotes as loci. We obtained ISSR data from ca. 60 phenotypically characterised Capsella bursa pastoris L. Medic (shepherds purse)accessions that had been isolated from a diverse mix of arable field sites throughout the UK. We developed mathematical scripts for use with the free statistical software tool R (http://www.rproject.org/), that processed the molecular data in a binary format to estimate genetic diversity (using the Jaccard co-efficient), and that related genotype to the plant phenotypic and environmental (site specific) traits. The methodology established has the power to predict the relationship between environmental and plant morphological characteristics

The Impact of Expanded, Multimodal Applications during a German Lied Performance

This study explored the impact of adaptive methods and modalities in Art Song performance in connecting with adult audiences (aged 18 and older) as explored through a live performance of German Lieder. In addition, this study sought to contribute a qualitative experimental study of in-situ audience perception to an emerging field of practice and research regarding the innovative practices in Art Song performance (e.g. Art Song Theatre). The study was derived from a 2012 informal experiment by Professor Rena Sharon, a leading advocate of expanded Art Song performance practice and pedagogy. Specifically, a single English Art Song was performed in three different treatments: traditional, expanded traditional, and theatrical contextualization. The data helped illustrate a difference in performer interpretation and audience comprehension, confirming a stronger affiliation to the more expansive treatments over the more normative one. Inspired by the anecdotal report of this experiment, the current study compared three gestural versions of Art Song performance, similar to the previously mentioned, on a larger scale (i.e. a German song cycle) and recorded the data through more extensive and systematic data collection before three distinct audiences, categorized according to level of prior classical music training and/or familiarity with Art Song. The conclusions observed provide further context on the effectiveness of innovative performance practices within classical music, such as Art Song Theatre, as well as suggest a set of parameters when putting forth a classical music performance – specifically that of Art Song/German Lied – for present-day audiences

Why bean beer?

Beer can be a wholesome beverage, and the art of brewing beer has been intertwined with the development of civilised society for centuries. Today, the latest valuation of the economic value of beer (by accountants Ernst and Young in 2013), reported that Europe is the world’s biggest producer of beer with over 4,500 breweries delivering around 390 million hectolitres annually – which in plain English is 68,632,200,000 pints (since 1 hectolitre is a small spillage less than 176 imperial pints). The industry employs over 2 million people, and around 125,000 of these are employed in breweries themselves. It should also be no surprise then that sales generated 53 billion Euro, which is almost doubled again by the value added from the supply chain. Also, the EU brewing sector had a trade surplus (i.e. exports were greater than imports) to the value of 3 billion Euro in 2012. Beer is serious business

TRUE - Transition Paths to Sustainable Legume Base Systems in Europe: European Countries and Kenya

info:eu-repo/semantics/publishedVersio

Influência do sobrepeso sobre a Estrutura de Sustentação Osteomuscular

In this century, among the numerous chronic diseases, we highlight obesity and osteoporosis as a result of the growing and high prevalence which are now considered epidemic and two major public health problems. There is little multidisciplinary research related to musculoskeletal clinical symptoms in patients with overweight and simultaneously evaluating the state of the musculoskeletal support structure and bone composition. The New Biology Bone, from an early age enables to trace the two bone matrix (protein and inorganic) that make up the support structure of the entire musculoskeletal system. Nowadays you can trace comprehensively bone protein matrix and detect early changes resulting from the habits, drugs, idleness, hormone deficiencies, obesity, chronic diseases among others. The old understanding that obesity exerts protective action to fractures was commuted by research in molecular level, which found that adipocytes and osteoblasts share the same progenitor cell. Objective: To trace the two bone matrix and compare the pattern of bone support structure in healthy young adults and on overweight. Result: The comparison showed that both qualitative tools accused significant difference at p <0.001. Stratification of UBPI tool revealed bone quality was poor in 7.7% of the patients with overweight and none in the control group. Stratification Profile Biophysical Bone (PBO) detected in 43.1% of overweight with Inverse Profile and none in the control group. Regarding quantitative tool (T-score) we didn´t detect differences in its three stratifications. Conclusion: In patients with overweight an early analysis of two bone-qualitative tools (PBO and UBPI) provide important information about the musculoskeletal support structure and enables the clinical nutrition specialist to develop preventive actions in the short term