Molecular and morphological phylogenetics of the digitate-tubered clade within subtribe Orchidinae s.s. (Orchidaceae: Orchideae)

The digitate-tubered clade (Dactylorhiza s.l. plus Gymnadenia s.l.) within subtribe Orchidinae is an important element of the North-temperate orchid flora and has become a model system for studying the genetic and epigenetic consequences of organism-wide ploidy change. Here, we integrate morphological phylogenetics with Sanger sequencing of nrITS and the plastid region trnL-F in order to explore phylogenetic relationships and phenotypic character evolution within the clade. The resulting morphological phylogenies are strongly incongruent with the molecular phylogenies, instead reconstructing through parsimony the genus-level boundaries recognised by traditional 20th Century taxonomy. They raise fresh doubts concerning whether Pseudorchis is sister to Platanthera or to Dactylorhiza plus Gymnadenia. Constraining the morphological matrix to the topology derived from ITS sequences increased tree length by 20%, adding considerably to the already exceptional level of phenotypic homoplasy. Both molecular and morphological trees agree that D. viridis and D. iberica are the earliest- diverging species within Dactylorhiza (emphasising the redundancy of the former genus Coeloglossum). Morphology and ITS both suggest that the former genus Nigritella is nested within (and thus part of) Gymnadenia, the Pyrenean endemic 'N.' gabasiana apparently forming a molecular bridge between the two radically contrasting core phenotypes. Comparatively short subtending molecular branches plus widespread (though sporadic) hybridisation indicate that Dactylorhiza and Gymnadenia approximate the minimum level of molecular divergence acceptable in sister genera. They share similar tuber morphologies and base chromosome numbers, and both genera are unusually prone to polyploid speciation. Another prominent feature of multiple speciation events within Gymnadenia is floral paedomorphosis. The 'traditional' morphological and candidate-gene approaches to phylogeny reconstruction are critically appraised.Peer reviewedFinal Published versio

Personal and social correlates of types of drug abuse in Dublin.

Significant differences were found between opioid takers and non-takers among referrals to Jervis Street Drug Advisory and Treatment Centre, Dublin, for the following variables: Sex, Age, Employment Status, Prior Court Appearance, Solitary drug taking. First drug taken abroad. Injection history. Physical complications associated with drug taking, and Maternal psychiatric status (for referrals aged under 21). Further analysis indicated 6 types of abuse associated with particular personal and social circumstances: (1) Minor tranquillisers, (2) Barbiturates only or with minor tranquillisers, (3) Opioids only or mainly, (4) Cannabis and/or L.S.D. only, (5) Poly-abuse excluding opioids, and (6) Poly-abuse including opioids.In broad social terms, these 6 groups may be seen as representing psychiatric or personal inadequacy-groups (1), (2) and (3), subcultural deviance-(5) and (6), and subcultural hedonism in the context of poor family relationships-(4). Groups most at risk were considered to be in the following order-(2), (3), (6),(1). Finally, cluster analyses, using unweighted and weighted variables, were employed to test the significance of distinctions based on types (3), (4), (5) and (6). In general, similar groups were obtained

Nature of behaviours that challenge in residents living in aged care homes: implications for psychosocial interventions and service development

Background/Aims: Competing theories exist regarding the causes and nature of behaviours that challenge (BC). The present study attempted to provide better insight into these behaviours and determine whether there were any underlying relationships for people living in care homes with and without dementia. Methods: Cross sectional data for 2185 residents living in 63 UK care homes using the Challenging Behaviour Scale were collected. The incidence (i.e. presence/absence) of 25 commonly reported behaviours that challenge staff were determined using dichotomous scoring. Cluster analysis and Mokken scaling were used to examine underlying relationships. Mokken is a method of analysing data to determine whether there is a ‘latent’ structure within a data set. Results: The prevalence of reported BC was 87.5%. Cluster analysis revealed three main clusters: apathy, agitation with internal focus, agitation with active external focus. For seven of the 25 items a hierarchical model emerged, where behaviours at the bottom of the hierarchy tended to occur in the presence of those higher up. Behaviours at the bottom of the hierarchy (dangerous behaviour) were less frequently observed than the items at the top (lack of self-care, verbal aggression). Conclusion: Some common BC may occur in groups and themed clusters. This study has shown that a hierarchical structure of BC in residents may be present. The findings testify to the complexity in the aetiology and treatment of BC and hence the need for focussed high intensity bio-psychosocial interventions to be targeted towards those with high levels of ‘unmet need’. Implications for future research and practice are discussed

Systematic review of topical treatments for fungal infections of the skin and nails of the feet

OBJECTIVE: To identify and synthesise the evidence for efficacy and cost effectiveness of topical treatments for superficial fungal infections of the skin and nails of the feet. DESIGN: Systematic review. INTERVENTIONS: Topical treatments for superficial fungal infections. MAIN OUTCOME MEASURES: Cure confirmed by culture and microscopy for skin and by culture for nails in patients with clinically diagnosed fungal infections. RESULTS: Of 126 trials identified in 121 papers, 72 (57.1%) met the inclusion criteria. Placebo controlled trials yielded pooled relative risks of failure to cure skin infections: allylamines (0.30, 95% confidence interval 0.24 to 0.38); azoles (0.54, 0.42 to 0.68); undecenoic acid (0.28, 0.11 to 0.74); and tolnaftate (0.46, 0.17 to 1.22). Although meta-analysis of 11 trials comparing allylamines and azoles showed a relative risk of failure to cure of 0.88 (0.78 to 0.99) in favour of allylamines, there was evidence of language bias. Seven reports in English favoured allylamines (0.79, 0.69 to 0.91), but four reports in foreign languages showed no difference between the two drugs (1.01, 0.90 to 1.13). Neither trial of nail infections showed significant differences between alternative topical treatments. CONCLUSIONS: Allylamines, azoles, and undecenoic acid were efficacious in placebo controlled trials. There are sufficient comparative trials to judge relative efficacy only between allylamines and azoles. Allylamines cure slightly more infections than azoles but are much more expensive than azoles. The most cost effective strategy is first to treat with azoles or undecenoic acid and to use allylamines only if that fails

Irish drug abusers II: their psychological characteristics.

This is the second in a series of 3 articles based on a study of a cohort of Irish drug abusers. This article attempts to identify the psychological traits of drug abusers within the cohort. The cohort consists of 100 drug abusers who attend a drug advisory and treatment centre in Dublin for the first time between November 1977 and February 1979. Three psychosocial, scales (GEFT, NSQ, and PFS) were administered to each subject. Overall, the results indicate that subjects who participated in the study, while of average or above average intelligence, were poorly psychologically adjusted, characterised by a poor sense of identity, a high level of suggestibility and a tendency towards emotional dependence. Their overall level of intelligence was within or above the normal range

Factors Relating to Reconviction among Young Dublin Probationers. General Research Series Paper No. 76, August 1974

THE approach adopted was to test and follow up 15° young Dublin probationers to discover factors associated with relapse into crime. A separate group of 57 non-attenders at school were studied for comparative purposes. Fifty-eight per cent of the probationers relapsed into crime, 35 per cent being committed to an institution