Assessment of the water quality of ten Waikato lakes based on zooplankton community composition. CBER Contract Report No. 60

Zooplankton communities were documented from ten Waikato lakes from net haul samples collected in late 2006 and from species hatched from diapausing eggs in sediments collected in early 2007. Lake trophic state was inferred based on the rotifer assemblages observed, and these inferred values were compared with predetermined water quality gradients assessed based on a limited dataset by Environment Waikato

Detection and distribution of Craspedacusta sowerbii: Observations of medusae are not enough

The freshwater cnidarian Craspedacusta sowerbii, native to the Yangtze valley, has invaded lakes and ponds throughout the world. Most distribution records have to date been based on observations of the medusa (jellyfish) stage, including numerous recent publications. We aimed to determine whether polyps are widespread in lakes, and geographical areas, outside of where medusae have been observed, and whether constructed waters are more easily invaded than natural waters. Our results show that C. sowerbii is more common and widespread than is apparent from observations of medusae. We argue that observed occurrences of medusae provide little useful information regarding the distribution of this species, and that published records of new jellyfish occurrences provide unreliable estimates of the timing of introduction, establishment or spread of C. sowerbii in new regions. We found no evidence that constructed waters were more readily invaded than natural waters. Overall, accurate determination of Craspedacusta occurrence and distribution requires systematic surveys of the polyp stages

Identifying invertebrate invasions using morphological and molecular analyses: North American Daphnia ‘pulex’ in New Zealand fresh waters

We used a DNA barcoding approach to identify specimens of the Daphnia pulex complex occurring in New Zealand lakes, documenting the establishment of non-indigenous North American Daphnia 'pulex'. Morphological delineation of species in this complex is problematic due to a lack of good morphological traits to distinguish the species, as there is a relatively high degree of morphological stasis within the group through evolutionary time. Accordingly, genetic analyses were used to determine the specific identity and likely geographic origin of this species. Morphologically, individuals most closely resembled Daphnia pulicaria or Daphnia pulex sensu lato, which cannot be separated morphologically. Furthermore, each of these taxa comprises separate species in North America and Europe, despite carrying the same names. We identified individuals using a 658 bp nucleotide portion of the mitochondrial cytochrome c oxidase subunit 1 gene (COI) as North American Daphnia 'pulex', being distinct from European Daphnia pulex sensu stricto and D. pulicaria from Europe or North America. Cellulose allozyme electrophoresis was used to confirm that individuals were not hybrids with D. pulicaria. North American Daphnia 'pulex' in New Zealand were first recorded in New Zealand from South Island lakes that are popular for overseas recreational fishers, indicating a possible source of introduction for this species (e.g. on/in fishing gear). Our study provides an additional example of how genetic techniques can be used for the accurate identification of non-indigenous taxa, particularly when morphological species determination is not possible. The growth of global databases such as GenBank and Barcode of Life Datasystems (BOLD) will further enhance this identification capacity

Wild record of an apple snail in the Waikato River, Hamilton, New Zealand and their incidence in freshwater aquaria

We report the discovery of a single specimen of a live apple snail Pomacea diffusa Blume 1957 (Ampullariidae: Prosobranchia), from the Waikato River, Hamilton city, central North Island, New Zealand. This species, along with the congeneric P. insularum, is imported for the aquarium trade, and its occurrence in the river likely stemmed from an aquarium release. A survey of 55 aquaria belonging to 43 hobbyists revealed 27 apple snails, with one owner having 22 snails. Assessment of environmental tolerances and impacts of P. diffusa, based largely on studies of the closely related and commonly confused congener P. bridgesii, suggests that direct habitat impacts by this species are likely to be minor. However, there could be indirect influences on native biodiversity through predation on eggs or competition for food supplies with other detritivorous species if densities were to become high. Water temperatures in the Waikato River below Hamilton (10-23˚C in 2009) may enable released individuals to persist for an extended period, and over summer may exceed the threshold required to enable breeding. However, population establishment would be most likely in locations where water is heated through geothermal influences or industrial cooling water discharges

Correspondence between zooplankton assemblages and the Estuary Environment Classification system

We tested whether variability in zooplankton assemblages was consistent with the categories of estuarine environments proposed by the 'Estuary Environment Classification' system (EEC) (Hume et al., 2007) across a variety of North Island, New Zealand, estuaries. The EEC classifies estuaries in to eight categories (A to F) based primarily on a combination of three abiotic controlling factors: ocean forcing, river forcing and basin morphometry. Additionally, we tested whether Remane's curve, which predicts higher diversities of benthic macrofauna and high and low salinities, can be applied to zooplankton assemblages. We focused on three of the eight EEC categories (B, D and F), which covered the range of estuaries with river inputs dominating (B) to ocean influence dominating (F). Additionally, we included samples from river (FW) and sea (MW) to encompass the entire salinity range. Zooplankton assemblages varied across the categories examined in accordance with a salinity gradient predicted by the EEC. Three groups of zooplankton were distinguishable: the first formed by the most freshwater categories, FW and B, and dominated by rotifers (primarily Bdelloidea) and estuarine copepods (Gladioferans pectinatus), a second group formed by categories D and F, of intermediate salinity, dominated by copepods (Euterpina acutifrons), and a final group including the purely marine category MW and dominated also by E. acutifrons along with other marine taxa. Zooplankton diversity responded to the salinity gradient in a manner expected from Remane's curve. The results of this study support others which have shown salinity to be the main factor driving zooplankton community composition and diversity. (C) 2016 Elsevier Ltd. All rights reserved.Peer reviewe

Skistodiaptomus pallidus (Copepoda: Diaptomidae) establishment in New Zealand natural lakes, and its effects on zooplankton community composition

The North American calanoid copepod Skistodiaptomus pallidus is an emerging invader globally, with non-indigenous populations recorded from constructed waters in New Zealand, Germany and Mexico since 2000. We examined the effects of S. pallidus establishment on the zooplankton community of a natural lake, Lake Kereta, where it was first recorded in late-2008, coincident with releases of domestically cultured grass carp (Ctenopharyngodon idella). Although not present in any of our samples prior to August 2008, S. pallidus was found in all samples collected in the subsequent five years. ANOSIM indicated zooplankton community composition significantly differed between samples collected before and after S. pallidus invasion, whether the invader was included in the analysis or not. Zooplankton species affected most greatly were the copepods Calamoecia lucasi and Mesocyclops sp., which decreased in their relative importance, and the cladocerans Bosmina meridionalis and Daphnia galeata, which increased. Rotifer species were relatively unaffected. As the length of grass carp released were >6.5 cm, direct predatory effects by this species on the zooplankton community are unlikely. Associated reductions in macrophyte biomass could explain increases in the relative abundances of planktonic cladocerans (B. meridionalis and D. galeata). However, the effect of macrophyte reduction by grass carp on zooplankton communities is considered to be limited elsewhere, while the reduced macrophyte biomass cannot explain the decrease in relative abundance of the native planktonic calanoid copepod C. lucasi. Competition between C. lucasi and S. pallidus is the most compelling explanation for the reduction in importance of the native calanoid copepod species. Skistodiaptomus pallidus appears to have undergone a “boom-and-bust” cycle in Lake Kereta, increasing in relative abundance in the first three years following establishment, before declining in importance

Development and evaluation of AGNI : a distributed netgraph data collection and analysis tool

Thesis (M.Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1998.Includes bibliographical references (leaves 46-47).AGNI is a tool developed to facilitate the analysis of communication networks between people in large organizations. Determining patterns of communication within organiza­tions is critical to the analysis of the effectiveness of their structure. Until recently, large organizations presented a special problem. The copious amounts of data that had to be analyzed made the process slow and tedious. AGNI makes the job easier by automating many of the tasks involved in this process. It provides a user friendly graphical environ­ment in which data can be collected and analyzed. It also provides an easy way to display the data in a graphical format so that it is easy to visualize.by Ian J.C. Duggan.M.Eng

Invasion risk of active and diapausing invertebrates from residual ballast in ships entering Chesapeake Bay

ABSTRACT: We examined the invasion risk posed by active invertebrates and their diapausing stages (e.g. resting eggs, quiescent adults) carried in residual sediment and water of non-ballasted ships to Chesapeake Bay. Many taxa were recorded that are not native to Chesapeake Bay, supporting the contention that residual ballast represents an invasion vector of some risk to marine systems. Composition and propagule supply differed relative to that in ships entering the Laurentian Great Lakes (e.g. marine taxa dominated in Chesapeake Bay ships), indicating that risk varies geographically. Average abundances of active invertebrates in residual sediment (1002.1 ind. kg–1) and water (2.7 ind. l–1), and diapausing eggs in sediments (779.4 eggs kg–1), were typically low relative to those in ships entering the Great Lakes (1322.5 ind. kg–1, 10.9 ind. l–1 and 3650.0 eggs kg–1, respectively). However, due to high variability among ships, differences were not statistically significant. The major cause of composition and abundance differences is dissimilar trade routes between each system, with vessels entering Chesapeake Bay primarily originating from marine rather than freshwater ports, and because diapausing stages are less commonly found among marine invertebrates. Low propagule supplies, predominant intra-continental ship movements, and salinity disparity between the upper (20 to 28‰) and lower (3 to 8‰) regions of Chesapeake Bay (where ballast water is loaded and offloaded) may greatly reduce invasion risk and be a contributing factor to the bay’s low invasion rate: invasion risk from non-ballasted ships here may be low relative to hull fouling or ballast water discharge. Other marine coastal areas may be at greater risk from this vector

In situ hatching of invertebrate diapausing eggs from ships’ ballast sediment

Ships that enter the Great Lakes laden with cargo carry only residual ballast water and sediment in ballast tanks. These ships are designated ‘no ballast on board’ (NOBOB) and constitute > 90% of inbound traffic. We conducted in situ experiments using emergence traps to assess the viability and the introduction potential of invertebrate diapausing stages present in ships’ ballast sediment. All trials commenced while vessels operated on the lower lakes (Erie, Ontario) and were completed 6–11 days later at ports on the upper lakes (Michigan, Lake Superior). Eight trials were conducted on four ships using five different ballast sediments. Hatching was observed on every ship, although not from all sediments on all ships. Overall hatch rates were very low (0.5 individuals per 500 g sediment), typically involving activation of < 0.05% of total eggs present. Five species of rotifers and copepod nauplii were hatched from ballast sediments, although only one or two species typically hatched from any one sediment. Results of this study indicate that hatching of diapausing eggs contained in ballast sediment of NOBOB ships poses a relatively low risk of invasion to the Great Lakes. However, as reproduction may occur in tanks, and non-indigenous species may be involved in numerous introduction events, the risk posed by this vector is small but potentially important. While dormancy is a characteristic enabling enhanced survival during transportation in ballast tanks, it becomes a hindrance for introduction.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72804/1/j.1366-9516.2005.00150.x.pd