Expression of proliferation-dependent antigens during cellular ageing of normal and progeroid human fibroblasts

Normal human fibroblasts display a limited lifespan in culture, which is due to a steadily decreasing fraction of cells that are able to proliferate. Using antibodies that react with antigens present in proliferating cells only, in an indirect immunofluorescence assay, we have estimated the fraction of proliferating cells in cultures of normal human fibroblasts. Furthermore, we have estimated the rate of decline in the fraction of proliferating cells during the process of cellular ageing by application of the assay to normal human fibroblasts throughout their lifespan in culture. Werner’s Syndrome is an autosomal recessive disease in which individuals display symptoms of ageing prematurely. Werner’s Syndrome fibroblasts display a reduced lifespan in culture compared with normal human fibroblasts. Like normal human fibroblasts, the growth of Werner’s Syndrome fibroblasts is characterised by a decreasing fraction of cells reacting with the proliferationassociated antibodies throughout their lifespan in culture. However, the rate of loss of proliferating cells in Werner’s Syndrome fibroblasts during the process of cellular ageing is accelerated 5- to 6-fold compared with the rate determined for normal human fibroblasts

A Bayesian approach towards daily pan-Arctic sea ice freeboard estimates from combined CryoSat-2 and Sentinel-3 satellite observations

Observations of sea ice freeboard from satellite radar altimeters are crucial in the derivation of sea ice thickness estimates, which in turn provide information on sea ice forecasts, volume budgets, and productivity rates. Current spatio-temporal resolution of radar freeboard is limited as 30 d are required in order to generate pan-Arctic coverage from CryoSat-2 and 27 d are required from Sentinel-3 satellites. This therefore hinders our ability to understand physical processes that drive sea ice thickness variability on sub-monthly timescales. In this study we exploit the consistency between CryoSat-2, Sentinel-3A, and Sentinel-3B radar freeboards in order to produce daily gridded pan-Arctic freeboard estimates between December 2018 and April 2019. We use the Bayesian inference approach of Gaussian process regression to learn functional mappings between radar freeboard observations in space and time and to subsequently retrieve pan-Arctic freeboard as well as uncertainty estimates. We also employ an empirical Bayesian approach towards learning the free (hyper)parameters of the model, which allows us to derive daily estimates related to radar freeboard spatial and temporal correlation length scales. The estimated daily radar freeboard predictions are, on average across the 2018–2019 season, equivalent to CryoSat-2 and Sentinel-3 freeboards to within 1 mm (standard deviations <6 cm), and cross-validation experiments show that errors in predictions are, on average, ≤ 4 mm across the same period. We also demonstrate the improved temporal variability of a pan-Arctic daily product by comparing time series of the predicted freeboards, with 31 d running means from CryoSat-2 and Sentinel-3 freeboards, across nine sectors of the Arctic, as well as making comparisons with daily ERA5 snowfall data. Pearson correlations between daily radar freeboard anomalies and snowfall are as high as +0.52 over first-year ice and +0.41 over multi-year ice, suggesting that the estimated daily fields are able to capture real physical radar freeboard variability at sub-weekly timescales

Absorption modes of Möbius strip resonators

This is the final version. Available on open access from Nature Research via the DOI in this record. In this work, the electromagnetic response of a mathematically interesting shape-a Möbius strip-is presented, along with a ring resonator for comparison. Both resonators consist of a central lossy dielectric layer bounded by perfectly conducting layers. For the case of the Möbius strips, the computational results show that there are a family of half-integer wavelength modes within the dielectric layer. These additional modes result in increased absorption, and a corresponding reduction in the radar cross section. Interestingly, rotational scans show that these modes can be excited over a large angular range. This investigation gives an understanding of the electromagnetic response of these structures, paving the way for future experiments on Möbius strip resonators.Engineering and Physical Sciences Research Council (EPSRC

Refractive errors in children with autism in a developing country

Background: In a resource.limited country visual problems of mentally challenged individuals are often neglected.Aim: The present study aims to study refractive errors in children diagnosed with autism in a developing country.Materials and Methods: Ophthalmic examination was carried out on children diagnosed with autism attending a school for the mentally  challenged in Enugu, Nigeria between December 2009 and May 2010. Visual acuity was assessed using Lea symbols. Anterior and posterior segments were examined. Cycloplegic refraction was performed. Datawas entered on the protocol prepared for the study and analyzed using Statistical Package for the Social Sciences version 17 (Chicago IL, USA).Results: A total of 21 children with autism were enrolled in the school; 18 of whom were examined giving coverage of 85.7%. The age range was 5.15 years, with a mean of 10.28 years (standard deviation } 3.20). There were 13 boys and 5 girls. One child had bilateral temporal pallor of the disc and one had bilateral maculopathy with diffuse chorioretinal atrophy.  Refraction revealed 4 children (22.2%) had astigmatism and 2 children (11.1%) had hypermetropia.Conclusion: Significant refractive error mainly astigmatism was noted in the children with autism. Identifying refractive errors in these children early and providing appropriate corrective lenses may help optimize their visual functioning and impact their activities of daily life in a positive way.Key words: Autism, developing country, refractive erro

Covert Images Using Surface Plasmon-Mediated Optical Polarization Conversion

This is the author accepted manuscript. The final version is available from Wiley via the DOI in this recordCovert optical signatures are a vital element in anticounterfeiting technologies. Plasmonic surfaces offer a means of manipulating the properties of light including the realization of colored pixels and images. In this work, concealed images with accurate color reproduction using plasmonic pixel arrays are demonstrated. The spectral and spatial control of optical polarization conversion is accomplished by tailoring the interaction of light with surface plasmons through the design and arrangement of surface nanostructures. The latent image is revealed using a polarization-sensitive optical system, which represents a means for the authentication of security features that can be created using these nanostructured devices. A red-green-blue color space is defined containing a wide gamut of chromaticities, enabling comprehensive full-color image capability. The device concept extends the functionality of a polarization-dependent plasmonic response to realize the encoding of a color image in covert form.This work was supported by the UK Engineering and Physical Sciences Research Council (EPSRC) Knowledge Transfer Account programme grant EP/H50012X/1, and by QinetiQ Ltd

Extending the Arctic Sea Ice Freeboard and Sea Level Record with the Sentinel-3 Radar Altimeters

In February 2016 and April 2018 the European Space Agency launched the Sentinel-3A and 3B satellites respectively, as part of the European Commission’s multi-satellite Copernicus Programme. Here we process Sentinel-3A waveform data to estimate Arctic sea level anomaly and radar freeboard from November 2017 to April 2018. We compare our results to those from the CryoSat-2 satellite, and find an intermission bias on sea-level anomaly of 2 cm. We also find a mean radar freeboard difference of 1 cm, which we attribute to the use of empirical retrackers to retrieve lead and floe elevations. Ahead of Sentinel-3B waveform data being made available, we use orbit files to estimate the improvement in sampling resolution afforded by the addition of Sentinel-3A and 3B data to the CryoSat-2 dataset. By combining data from the three satellites, grid resolution or time-sampling can be almost tripled compared with using CryoSat-2 data alone

Effective-periodicity effects in Fibonacci slot arrays (article)

This is the final version. Available from the American Physical Society via the DOI in this record. The data that support the findings of this study are available from the corresponding author upon reasonable request.The dataset associated with this article is available in ORE at: https://doi.org/10.24378/exe.3783In this Letter, the transmission properties of a nonperiodic array of slots arranged in the form of a Fibonacci sequence are investigated. By arranging the slots in this manner, an additional periodicity can be utilized, resulting in corresponding resonance features in the transmitted signal. By investigating the transmission response of a perforated metallic sheet over a broad frequency range (6–40 GHz), it is shown that this simple one-dimensional chain supports two periodicities, one due to the regular periodic separation and one due to average spacing—which is related to the golden ratio. This response replicates the resonant behavior of a two-dimensional periodic array with a single nonperiodic array also creating new families of diffraction lobes in the far-field region.Engineering and Physical Sciences Research CouncilEngineering and Physical Sciences Research CouncilMCIN/AEI/10.13039/50110001103

Efficient mm-wave photomodulation via coupled Fabry–Perot cavities

This is the author accepted manuscript. The final version is available from AIP Publishing via the DOI in this recordData availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.An efficient mm-wave photomodulator is designed based on coupled Fabry–Perot modes in a low-lifetime silicon wafer and an adjacent cavity formed from a transparent reflector, such as indium tin oxide. The modulation of a reflected beam using this coupled-cavity design is increased by a factor of 7 compared with that from an isolated silicon wafer, while also introducing a degree of tunability and maintaining low angular dispersion. For the particular design built and tested, a modulation of 32% is achieved for an extremely low optical illumination of just 0.006W/cm2 and with a maximum operation rate of more than 3 kHz. The large increase in modulation, coupled with the flexibility of the design and the fact that all components can be industrially manufactured, makes this photomodulator a promising candidate for many communication, imaging, and sensing applications.Engineering and Physical Sciences Research Council (EPSRC)QinetiQ Ltd

Inter-comparison of snow depth over Arctic sea ice from reanalysis reconstructions and satellite retrieval

In this study, we compare eight recently developed snow depth products over Arctic sea ice, which use satellite observations, modeling, or a combination of satellite and modeling approaches. These products are further compared against various ground-truth observations, including those from ice mass balance observations and airborne measurements. Large mean snow depth discrepancies are observed over the Atlantic and Canadian Arctic sectors. The differences between climatology and the snow products early in winter could be in part a result of the delaying in Arctic ice formation that reduces early snow accumulation, leading to shallower snowpacks at the start of the freeze-up season. These differences persist through spring despite overall more winter snow accumulation in the reanalysis-based products than in the climatologies. Among the products evaluated, the University of Washington (UW) snow depth product produces the deepest spring (March-April) snowpacks, while the snow product from the Danish Meteorological Institute (DMI) provides the shallowest spring snow depths. Most snow products show significant correlation with snow depths retrieved from Operational IceBridge (OIB) while correlations are quite low against buoy measurements, with no correlation and very low variability from University of Bremen and DMI products. Inconsistencies in reconstructed snow depth among the products, as well as differences between these products and in situ and airborne observations, can be partially attributed to differences in effective footprint and spatial-temporal coverage, as well as insufficient observations for validation/bias adjustments. Our results highlight the need for more targeted Arctic surveys over different spatial and temporal scales to allow for a more systematic comparison and fusion of airborne, in situ and remote sensing observations

Multi-resonant tessellated anchor-based metasurfaces

This is the final version. Available from Nature Research via the DOI in this record. Data availability: The research data supporting this publication are openly available from the University of Exeter’s institutional repository at: https://doi.org/10.24378/exe.4504.In this work, a multi-resonant metasurface that can be tailored to absorb microwaves at one or more frequencies is explored. Surface shapes based on an ‘anchor’ motif, incorporating hexagonal, square and triangular-shaped resonant elements, are shown to be readily tailorable to provide a targeted range of microwave responses. A metasurface consisting of an etched copper layer, spaced above a ground plane by a thin (<1/10th of a wavelength) low-loss dielectric is experimentally characterised. The fundamental resonances of each shaped element are exhibited at 4.1 GHz (triangular), 6.1 GHz (square) and 10.1 GHz (hexagonal), providing the potential for single- and multi-frequency absorption across a range that is of interest to the food industry. Reflectivity measurements of the metasurface demonstrate that the three fundamental absorption modes are largely independent of incident polarization as well as both azimuthal and elevation angles.Engineering and Physical Sciences Research Council (EPSRC)PepsiC