Whole genome sequencing of experimental hybrids supports meiosis-like sexual recombination in Leishmania

Hybrid genotypes have been repeatedly described among natural isolates of Leishmania, and the recovery of experimental hybrids from sand flies co-infected with different strains or species of Leishmania has formally demonstrated that members of the genus possess the machinery for genetic exchange. As neither gamete stages nor cell fusion events have been directly observed during parasite development in the vector, we have relied on a classical genetic analysis to determine if Leishmania has a true sexual cycle. Here, we used whole genome sequencing to follow the chromosomal inheritance patterns of experimental hybrids generated within and between different strains of L. major and L. infantum. We also generated and sequenced the first experimental hybrids in L. tropica. We found that in each case the parental somy and allele contributions matched the inheritance patterns expected under meiosis 97–99% of the time. The hybrids were equivalent to F1 progeny, heterozygous throughout most of the genome for the markers that were homozygous and different between the parents. Rare, non-Mendelian patterns of chromosomal inheritance were observed, including a gain or loss of somy, and loss of heterozygosity, that likely arose during meiosis or during mitotic divisions of the progeny clones in the fly or culture. While the interspecies hybrids appeared to be sterile, the intraspecies hybrids were able to produce backcross and outcross progeny. Analysis of 5 backcross and outcross progeny clones generated from an L. major F1 hybrid, as well as 17 progeny clones generated from backcrosses involving a natural hybrid of L. tropica, revealed genome wide patterns of recombination, demonstrating that classical crossing over occurs at meiosis, and allowed us to construct the first physical and genetic maps in Leishmania. Altogether, the findings provide strong evidence for meiosis-like sexual recombination in Leishmania, presenting clear opportunities for forward genetic analysis and positional cloning of important genes.</div

Menstrual cycle phase does not predict political conservatism

Recent authors have reported a relationship between women's fertility status, as indexed by menstrual cycle phase, and conservatism in moral, social and political values. We conducted a survey to test for the existence of a relationship between menstrual cycle day and conservatism. 2213 women reporting regular menstrual cycles provided data about their political views. Of these women, 2208 provided information about their cycle date, 1260 provided additional evidence of reliability in self-reported cycle date, and of these, 750 also indicated an absence of hormonal disruptors such as recent hormonal contraception use, breastfeeding or pregnancy. Cycle day was used to estimate day-specific fertility rate (probability of conception); political conservatism was measured via direct self-report and via responses to the "Moral Foundations” questionnaire. We also recorded relationship status, which has been reported to interact with menstrual cycle phase in determining political preferences. We found no evidence of a relationship between estimated cyclical fertility changes and conservatism, and no evidence of an interaction between relationship status and cyclical fertility in determining political attitudes. Our findings were robust to multiple inclusion/exclusion criteria and to different methods of estimating fertility and measuring conservatism. In summary, the relationship between cycle-linked reproductive parameters and conservatism may be weaker or less reliable than previously thought

Promoting independence in Lewy body dementia through exercise: the PRIDE study

Background: Lewy body dementia (LBD) is an aggressive type of dementia of rapid, fluctuating disease trajectory, higher incidence of adverse events, and poorer functional independence than observed in Alzheimer’s disease dementia. Non-pharmacological treatments such as progressive, high-intensity exercise are effective in other neurological cohorts but have been scarcely evaluated in LBD. Methods: The Promoting Independence in Lewy Body Dementia through Exercise (PRIDE) trial was a non-randomised, non-blinded, crossover pilot trial involving older adults with LBD consisting of a baseline assessment, an 8-week wait-list, and an 8-week exercise intervention. The aims of this study were to evaluate the determinants of the primary outcome functional independence, as measured by the Movement Disorder Society Unified Parkinson’s Disease Rating Scale, and the feasibility and preliminary efficacy of an exercise intervention on this outcome. Additionally, important clinical characteristics were evaluated to explore associations and treatment targets. The exercise intervention was supervised, clinic-based, high-intensity progressive resistance training (PRT), challenging balance, and functional exercises, 3 days/week. Results: Nine participants completed the baseline cross-sectional study, of which five had a diagnosis of Parkinson’s disease dementia (PDD), and four dementia with Lewy Bodies (DLB). Six completed the exercise intervention (three PDD, three DLB). The cohort was diverse, ranging from mild to severe dementia and living in various residential settings. Greater functional independence at baseline was significantly associated with better physical function, balance, cognition, quality of life, muscle mass ratio, walking endurance, faster walking speed and cadence, and lower dementia severity (p 80% adherence), and only one minor exercise-related adverse event occurred. Conclusions: PRIDE is the first exercise trial conducted specifically within individuals diagnosed with LBD, and provides important insight for the design of larger, randomized trials for further evaluation of progressive, high-intensity exercise as a valuable treatment in LBD. Trial registration: The PRIDE trial protocol has previously been prospectively registered (08/04/2016, ANZCTR: ACTRN12616000466448)

Effects of pressure on diffusion and vacancy formation in MgO from non-empirical free-energy integrations

The free energies of vacancy pair formation and migration in MgO were computed via molecular dynamics using free-energy integrations and a non-empirical ionic model with no adjustable parameters. The intrinsic diffusion constant for MgO was obtained at pressures from 0 to 140 GPa and temperatures from 1000 to 5000 K. Excellent agreement was found with the zero pressure diffusion data within experimental error. The homologous temperature model which relates diffusion to the melting curve describes well our high pressure results within our theoretical framework.Comment: 4 pages, latex, 1 figure, revtex, submitted to PR

Comparison of the Electronic Structures and Energetics of Ferroelectric LiNbO3 and LiTaO3

This paper explains the origin of the ferroelectric instability in LiNbO3 and LiTaO3 and compares the electronic structures and energetics of the two materials.Comment: 31 pages, 11 Postscript figure

Benefiting From Misfortune: When Harmless Actions Are Judged to Be Morally Blameworthy

Dominant theories of moral blame require an individual to have caused or intended harm. However, the current four studies demonstrate cases where no harm is caused or intended, yet individuals are nonetheless deemed worthy of blame. Specifically, individuals are judged to be blameworthy when they engage in actions that enable them to benefit from another’s misfortune (e.g., betting that a company’s stock will decline or that a natural disaster will occur). Evidence is presented suggesting that perceptions of the actor’s wicked desires are responsible for this phenomenon. It is argued that these results are consistent with a growing literature demonstrating that moral judgments are often the product of evaluations of character in addition to evaluations of acts

Evaluation of multiple protein docking structures using correctly predicted pairwise subunits

<p>Abstract</p> <p>Background</p> <p>Many functionally important proteins in a cell form complexes with multiple chains. Therefore, computational prediction of multiple protein complexes is an important task in bioinformatics. In the development of multiple protein docking methods, it is important to establish a metric for evaluating prediction results in a reasonable and practical fashion. However, since there are only few works done in developing methods for multiple protein docking, there is no study that investigates how accurate structural models of multiple protein complexes should be to allow scientists to gain biological insights.</p> <p>Methods</p> <p>We generated a series of predicted models (decoys) of various accuracies by our multiple protein docking pipeline, Multi-LZerD, for three multi-chain complexes with 3, 4, and 6 chains. We analyzed the decoys in terms of the number of correctly predicted pair conformations in the decoys.</p> <p>Results and conclusion</p> <p>We found that pairs of chains with the correct mutual orientation exist even in the decoys with a large overall root mean square deviation (RMSD) to the native. Therefore, in addition to a global structure similarity measure, such as the global RMSD, the quality of models for multiple chain complexes can be better evaluated by using the local measurement, the number of chain pairs with correct mutual orientation. We termed the fraction of correctly predicted pairs (RMSD at the interface of less than 4.0Å) as <it>fpair </it>and propose to use it for evaluation of the accuracy of multiple protein docking.</p

Ornamental plants: a summary of research, 1990

Physical facilities and capital requirements for establishing a three-acre perennial container nursery in Ohio -1989 / Reed D. Taylor, Elton M. Smith, George P. Pealer -- Production costs of operating a three-acre perennial container nursery in Ohio - 1989 / Reed D. Taylor, Elton M. Smith, George P. Pealer -- Comparative costs of producing alternative plants in a three-acre perennial container nursery in Ohio – 1989 / Reed D. Taylor, Elton M. Smith, George P. Pealer -- Marketing and sales strategies practiced by Ohio nurseries / Reed D. Taylor, Elton M. Smith, W. Timothy Rhodus -- Evaluation of flowering crabapple susceptibility to apple scab in Ohio - 1989 / Elton M. Smith and Sharon A. Treaster -- Fertilizing trees in the landscape: an 18-year evaluation / Elton M. Smith and Sharon A. Treaster -- The influence of irrigation water temperature upon leaf abscision in Ficus benjamina and Dracaena marginata / Julia A. O’Bryan and John C. Peterson -- A two-year tolerance study of narcissus cultivars to selected pre-emergence herbicides / Elton M. Smith and Sharon A. Treaster -- Tolerance of clematis and wisteria to Ronstar and Devrinol / Elton M. Smith and Sharon A. Treaster -- Increasing efficiency of slow-release herbicide tablets / Elton M. Smith and Sharon A. Treaster -- Developing biorational pesticides for the landscape industry / David G. Nielsen -- Interactions between subdue and slow-release fertilizers at high temperatures / Y. Inbar, H. A. J. Hoitink, M. E. Watson, K. D. Cochran, E. M. Smith -- Evaluation of powdery mildew severity on deciduous azaleas at the Secrest Arboretum - 1989 / Kenneth D. Cochran and C. Wayne Ellett -- Winter damage, regrowth and vigor of old garden roses / Gary A. Anderso

Bench behaviour of ice hockey coaches: Psychophysiological and verbal responses to critical game incidents

The purpose of this study was to examine coaches’ psychophysiological and verbal responses to different game situations. The in-game heart rate and verbal responses of three elite ice hockey coaches to four critical game incidents (Goals For/Against; Penalties Taken/Drawn) over four university women’s games were assessed. Verbal comments were categorised using the Coach Behaviour Assessment System, and then comments and heart rate were sequenced to critical incidents recorded on video review. Overall, in-game heart rate was greater than rest and coaches were rarely silent. General encouragement and general commentary were the most common verbal comments. Two hundred and eight critical incident comments were recorded (Goals For/Against 34.6 %; Penalties Taken/Drawn 65.4%) associated with a 10 bpm greater heart rate. Most common verbal responses to critical incidents were general commentary, silence and organisation. The type of comment was affected by the type of critical incident. In 78% of critical incidents, the type of comment made before incidents differed to type of comment after the incident, coaches rarely talked at the same time and silence was common. These novel findings are limited to ice hockey coaches given the small sample size. However, these results should encourage more research into the psychophysiological and verbal responses of coaches in other team sports real game situations to better understand in game coaching behaviour