Expression of pathogenesis related genes in response to salicylic acid, methyl jasmonate and 1-aminocyclopropane-1-carboxylic acid in Malus hupehensis (Pamp.) Rehd

<p>Abstract</p> <p>Background</p> <p>Many studies have been done to find out the molecular mechanism of systemic acquired resistance (SAR) in plants in the past several decades. Numbers of researches have been carried out in the model plants such as arabidopsis, tobacco, rice and so on, however, with little work done in woody plants especially in fruit trees such as apple. Components of the pathway of SAR seem to be extremely conserved in the variety of species. <it>Malus hupehensis</it>, which is origin in China, is strong resistance with rootstock. In the study, we attempted to make the expression pattern of pathogenesis related (PR) genes which were downstream components of the SAR pathway in response to salicylic acid(SA), methyl jasmonate(MeJA) and 1-aminocyclopropane-1-carboxylic acid(ACC) in <it>Malus hupehensis</it>.</p> <p>Findings</p> <p>In order to analyze the expression pattern, the partial sequence of three PR genes from <it>Malus hupehensis</it>, <it>MhPR1</it>, <it>MhPR5 </it>and <it>MhPR8 </it>was isolated. These three PR genes were induced by SA, MeJA and ACC. However, <it>MhPR1</it>, <it>MhPR5 </it>and <it>MhPR8 </it>performed a distinct pattern of expression in different plant organs. <it>MhPR5 </it>and <it>MhPR8 </it>were basal expression in leaves, stems and roots, and <it>MhPR1 </it>was basal expression only in stems. The expression of <it>MhPR1</it>, <it>MhPR5 </it>and <it>MhPR8 </it>was enhanced during the first 48 h post-induced with SA, MeJA and ACC.</p> <p>Conclusions</p> <p>The results showed that a distinct pattern of expression of PR genes in <it>Malus hupehensis </it>which differed from the previous reports on model plants arabidopsis, tobacco and rice. <it>MhPR1</it>, <it>MhPR5 </it>and <it>MhPR8 </it>were induced by SA, MeJA and ACC, which were regarded as the marker genes in the SAR response in <it>Malus hupehensis</it>. In contrast with herbal plants, there could be specific signal pathway in response to SA, JA and ET for woody plants.</p

State-of-the-art three-dimensional analysis of soft tissue changes following Le Fort I maxillary advancement

We describe the comprehensive 3-dimensional analysis of facial changes after Le Fort I osteotomy and introduce a new tool for anthropometric analysis of the face. We studied the cone-beam computed tomograms of 33 patients taken one month before and 6-12 months after Le Fort I maxillary advancement with or without posterior vertical impaction. Use of a generic facial mesh for dense correspondence analysis of changes in the soft tissue showed a mean (SD) anteroposterior advancement of the maxilla of 5.9 (1.7) mm, and mean (SD) minimal anterior and posterior vertical maxillary impaction of 0.1 (1.7) mm and 0.6 (1.45) mm, respectively. It also showed distinctive forward and marked lateral expansion around the upper lip and nose, and pronounced upward movement of the alar curvature and columella. The nose was widened and the nostrils advanced. There was minimal forward change at the base of the nose (subnasale and alar base) but a noticeable upward movement at the nasal tip. Changes at the cheeks were minimal. Analysis showed widening of the midface and upper lip which, to our knowledge, has not been reported before. The nostrils were compressed and widened, and the lower lip shortened. Changes at the chin and lower lip were secondary to the limited maxillary impaction

How do plants achieve immunity? Defence without specialized immune cells

Vertebrates have evolved a sophisticated adaptive immune system that relies on an almost infinite diversity of antigen receptors that are clonally expressed by specialized immune cells that roam the circulatory system. These immune cells provide vertebrates with extraordinary antigen-specific immune capacity and memory, while minimizing self-reactivity. Plants, however, lack specialized mobile immune cells. Instead, every plant cell is thought to be capable of launching an effective immune response. So how do plants achieve specific, self-tolerant immunity and establish immune memory? Recent developments point towards a multilayered plant innate immune system comprised of self-surveillance, systemic signalling and chromosomal changes that together establish effective immunity