UTILITY OF CERTAIN σ AND π-ACCEPTORS FOR THE SPECTROPHOTOMETRIC DETERMINATION OF VORICONAZOL ANTIFUGAL DRUG IN PHARMACEUTICAL FORMULATION

Objective: Studies were carried out, for the first time, to investigate the charge-transfer reactions of voriconazole antifungal drug (VOR) as n-electron donor with the σ-acceptor iodine (I2) and various Ï€-acceptors: 7,7,8,8-tetracyanoquinodimethane (TCNQ); 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) and p-chloranilic acid (p-CLA).Methods: The formation of the colored charge-transfer complexes were utilized in the development of simple, rapid and accurate spectrophotometric methods for the analysis of voriconazole in pure form as well as in its pharmaceutical formulation (tablets). Different variables affecting the reactions were studied and optimized.Results: Under the optimum reaction conditions, linear relationships with good correlation coefficients (0.9994–0.9999) were found between the absorbance and the concentration of voriconazole in the range of 2.0–120 μg mL−1. For more accurate analysis, Ringbom optimum concentration range was found to be between 4.0-110 μg mL-1. The limits of detection ranged from 0.36 to 1.23 μg mL−1andthe limits of quantification ranged from 1.20 to 4.10 μg mL−1. A Job's plot of the absorbance versus the molar ratio of voriconazole to each of acceptors under consideration indicated (1:1) ratio.Conclusion: The proposed methods were applied successfully for simultaneous determination of voriconazole in tablets with good accuracy and precision and without interferences from common additives. The results were compared favourably with the reported method.Â

GREEN VORTEX-ASSISTED IONIC LIQUID-BASED DISPERSIVE LIQUID-LIQUID MICROEXTRACTION FOR ENRICHMENT AND DETERMINATION OF CADMIUM AND LEAD IN WATER, VEGETABLES AND TOBACCO SAMPLES

Objective: An eco-friendly, simple and sensitive vortex-assisted ionic liquid-based dispersive liquid-liquid microextraction method (VA-IL-DLLµE) has been proposed to enrich and determine trace levels of cadmium (Cd2+ ) and lead (Pb2+ ) ions in water, vegetables and tobacco samples, prior to its FAAS determination. Methods: The proposed method based on utilization of ionic liquid (IL) (1-hexyl-3-methylimidazolium tris(pentafluoroethyl)trifluorophosphate [HMIM][FAP]) as an extraction solvent for both ions after the complexation with 4,5-dihydroxy-3-phenylazo-2,7-naphthalenedisulfonic acid, disodium salt (Chromotrope 2R) at pH 6.5. The impact of different analytical parameters on microextraction efficiency was optimized. Results: In the ranges of 1.0–300 and 2.0-400 μg/ml, the calibration graphs were linear. The limits of detection were 0.3 and 0.6 μg/ml for Cd2+ and Pb2+ ions, respectively. The preconcentration factor was 100. The relative standard deviation (RSD %)<3.0%, which indicates the proposed method has high precision. Conclusion: The proposed VA-IL-DLLµE method was developed and applied for the estimation of Cd2+ and Pb2+ ion content in various water, vegetables and tobacco samples, and satisfactory results were obtained. The obtained recovery values showed good agreement with the certified values

EFFECT OF BENZYLADENINE ON MICROPROPAGATION OF BANANA SHOOTS TIP

The effect of benzyl adenine (BA) at 0, 2, 4, 6 and 8 mg l-1 on micropropagation of banana shoot tips was studied. This study also included the morphological responses of banana shoot tips especially with 0 & 6 mg l-1 of BA treatments in relation to some biochemical compositions (total soluble phenols, free amino acids, total soluble sugars, chlorophyll a, b and carotenoids). Growth in 6  mg l-1 of BA resulted in increase in the most morphological parameters compared to the rest treatments. The results showed that 6 mg l-1 of BA treatment significantly increased fresh and dry weights, number of shootlets, shootlet and root length and number of leaves and roots/plantlet as compared without BA. Accumulation of total soluble sugars, free amino acids and chlorophylls was enhanced by 6 mg l-1 of BA while the reverse was true with the rest biochemical compositions (total soluble phenols and carotenoids). The biochemical status and BA treatment at 6 mg l-1 during micropropagation of shoot tips in banana may be important for the development and optimization of strategies for large scale propagation and germplasm conservation

Treatment of CandidaalbicansBiofilms – Associated with Dry Socket orDenture Stomatitis by PropolisPaste

Degradation of oral health is often assumed to progress with aging. However, significantly higher total counts and greater varieties of Candida species can be detected in wearers of removable dentures compared with nondenture wearers.Poorly fitting or unhygienic dentures leads to the presence of yeast like fungi (Candidaalbicans ) attached to it, and cause inflammation. Although candidiasis is highly resistant to antifungal agents, systemic drugs usage are necessary. But the systemic use of these drugs can cause side effects like liver toxicity, drug interactions etc.Usingpropolseasantifungal by localapplication, found that it suppresses the Candida associated dry socket and denture stomatitis. The Propolispast was used in the following manner. It was applied to the fitting surface of denture, twice a day for 2 weeks. The Propolisputty was putted in dry socket after complete removal of inflammatory tissuesfrom it.The results showed pain, redness area and inflammation related stomatitis were disappear gradually after 48 hours until 2 weeks that the denture stomatitis is healing by using a Propolispast.Propolisputty treat the fungal inflammation of dry socket that gradual decreases of the pain and inflammation.After the treatment, all patientswith denture stomatitis were subjected to examination of the palatal mucosa and socket and quantitative culture of Candida from the palatal mucosa and denture fitting surface and diagnosed by three method :Germ tube.GramStain.andChromAgar medi

Autoxidation of 4-Hydrazinylquinolin-2(1H)-one; Synthesis of Pyridazino[4,3-c:5,6-c′]diquinoline-6,7(5H,8H)-diones

An efficient synthesis of a series of pyridazino[4,3-c:5,6-c′]diquinolines was achieved via the autoxidation of 4-hydrazinylquinolin-2(1H)-ones. IR, NMR (1H and 13C), mass spectral data, and elemental analysis were used to fit and elucidate the structures of the newly synthesized compounds. X-ray structure analysis and theoretical calculations unequivocally proved the formation of the structure. The possible mechanism for the reaction is also discussed

Autoxidation of 4-Hydrazinylquinolin-2(1H)-one; Synthesis of Pyridazino[4,3-c:5,6-c′]diquinoline-6,7(5H,8H)-diones

An efficient synthesis of a series of pyridazino[4,3-c:5,6-c′]diquinolines was achieved via the autoxidation of 4-hydrazinylquinolin-2(1H)-ones. IR, NMR (1H and 13C), mass spectral data, and elemental analysis were used to fit and elucidate the structures of the newly synthesized compounds. X-ray structure analysis and theoretical calculations unequivocally proved the formation of the structure. The possible mechanism for the reaction is also discussed

Autoxidation of 4-Hydrazinylquinolin-2(1H)-one; Synthesis of Pyridazino[4,3-c : 5,6-c ']diquinoline-6,7(5H,8H)-diones

An efficient synthesis of a series of pyridazino[4,3-c:5,6-c']diquinolines was achieved via the autoxidation of 4-hydrazinylquinolin-2(1H)-ones. IR, NMR (H-1 and C-13), mass spectral data, and elemental analysis were used to fit and elucidate the structures of the newly synthesized compounds. X-ray structure analysis and theoretical calculations unequivocally proved the formation of the structure. The possible mechanism for the reaction is also discussed.Peer reviewe

Autoxidation of 4-Hydrazinylquinolin-2(1H)-one; Synthesis of Pyridazino[4,3-c:5,6-c′ ]diquinoline-6,7(5H,8H)-diones

An efficient synthesis of a series of pyridazino[4,3-c:5,6-c′]diquinolines was achieved via the autoxidation of 4-hydrazinylquinolin-2(1H)-ones. IR, NMR ($^1$H and $^13$C), mass spectral data, and elemental analysis were used to fit and elucidate the structures of the newly synthesized compounds. X-ray structure analysis and theoretical calculations unequivocally proved the formation of the structure. The possible mechanism for the reaction is also discussed

Insulin Receptor Substrate Adaptor Proteins Mediate Prognostic Gene Expression Profiles in Breast Cancer

Therapies targeting the type I insulin-like growth factor receptor (IGF-1R) have not been developed with predictive biomarkers to identify tumors with receptor activation. We have previously shown that the insulin receptor substrate (IRS) adaptor proteins are necessary for linking IGF1R to downstream signaling pathways and the malignant phenotype in breast cancer cells. The purpose of this study was to identify gene expression profiles downstream of IGF1R and its two adaptor proteins. IRS-null breast cancer cells (T47D-YA) were engineered to express IRS-1 or IRS-2 alone and their ability to mediate IGF ligand-induced proliferation, motility, and gene expression determined. Global gene expression signatures reflecting IRS adaptor specific and primary vs. secondary ligand response were derived (Early IRS-1, Late IRS-1, Early IRS-2 and Late IRS-2) and functional pathway analysis examined. IRS isoforms mediated distinct gene expression profiles, functional pathways, and breast cancer subtype association. For example, IRS-1/2-induced TGFb2 expression and blockade of TGFb2 abrogated IGF-induced cell migration. In addition, the prognostic value of IRS proteins was significant in the luminal B breast tumor subtype. Univariate and multivariate analyses confirmed that IRS adaptor signatures correlated with poor outcome as measured by recurrence-free and overall survival. Thus, IRS adaptor protein expression is required for IGF ligand responses in breast cancer cells. IRS-specific gene signatures represent accurate surrogates of IGF activity and could predict response to anti-IGF therapy in breast cancer