Serotonin Modulates Oscillations of the Membrane Potential in Isolated Spinal Neurons from Lampreys

Studies were performed on spinal neurons from lampreys isolated by an enzymatic/mechanical method using pronase. The effects of 100 µM serotonin (5-HT) on membrane potential oscillations induced by a variety of excitatory amino acids were studied. 5-HT was found to depolarize branched cells (presumptive motoneurons and interneurons) by 2–6 mV without inducing membrane potential oscillations. However, when oscillations were already present because of an excitatory amino acid, 5-HT changed the parameters of these oscillations, increasing the amplitudes of all types of oscillations, increasing the frequency of irregular oscillations, and increasing the duration of the depolarization plateaus accompanied by action potentials. Serotonin modulation of the effects of excitatory amino acids and the electrical activity of cells in the neural locomotor network facilitates motor activity and leads to increases in the contraction of truncal muscles and more intense movements by the animal. The possible mechanisms of receptor coactivation are discussed, along with increases in action potential frequency and changes in the parameters of the locomotor rhythm

The Effects of Serotonin on Functionally Diverse Isolated Lamprey Spinal Cord Neurons

The experiments reported here showed that application of serotonin (5-hydroxytryptamine, 5-HT) (100 µ M) did not induce any significant current through the membranes of any of the spinal neurons studied (n = 62). At the same time, the membranes of most motoneurons and interneurons (15 of 18) underwent slight depolarization (2–6 mV) in the presence of 5-HT, which was not accompanied by any change in the input resistance of the cells. Depolarization to 10–20 mV occurred in some cells (3 of 18) of these functional groups, this being accompanied by 20–60% decreases in input resistance. The same concentration of 5-HT induced transient low-amplitude depolarization of most sensory spinal neurons (dorsal sensory cells), this changing smoothly to long-term hyperpolarization by 2–7 mV. The input resistance of the cell membranes in these cases showed no significant change (n = 8). Data were obtained which provided a better understanding of the mechanism by which 5-HT modulates the activity of spinal neurons. Thus, 5-HT facilitates chemoreceptive currents induced by application of NMDA to motoneurons and interneurons, while the NMDA responses of dorsal sensory cells were decreased by 5-HT. 5-HT affected the post-spike afterresponses of neurons. 5-HT significantly decreased the amplitude of afterhyperpolarization arising at the end of the descending phase of action potentials in motoneurons and interneurons and increased the amplitude of afterdepolarization in these types of cells. In sensory spinal neurons, 5-HT had no great effect on post-spike afterresponses. The results obtained here support the suggestion that 5-HT significantly modulates the activity of spinal neurons of different functional types. 5-HT facilitates excitation induced by subthreshold depolarization in motoneurons and some interneurons, facilitating the generation of rhythmic discharges by decreasing afterhyperpolarization. In sensory cells, 5-HT enhances inhibition due to hyperpolarization, suppressing NMDA currents. The differences in the effects of 5-HT on functionally diverse neurons are presumed to be associated with the combination of different types of 5-HT receptors on the membranes of these types of spinal neurons

Quality Management in Pharmaceutical Development of Biotechnology-Derived Medicinal Products: Implementation and Evaluation

Scientific relevance. One of the challenges associated with the development of medicines lies in creating a quality management system (QMS) and tools to assess its performance.Aim. The study aimed to propose a QMS model for the development of biotechnology-derived medicinal products and a performance evaluation tool for this QMS universally applicable in the research centre of the company regardless of the specific activities of its individual divisions.Materials and methods. The study analysed internal audit outcomes, non-compliance responses, and the document flow of the research centre. Parameter values for quality index (QI) calculations were entered into validated Microsoft Excel spreadsheets. Data analysis and visualisation involved using the Microsoft Power Business Intelligence (BI) business analytics platform (mainly, the Power BI Desktop and Power BI Service components).Results. The QMS was implemented, and the authors proposed their QMS performance evaluation tool universally applicable to all the divisions of the research centre.Conclusions. The authors proposed their QMS model for the development of biotechnology-derived medicinal products and the QI tool for collecting digital data, carrying out standardised monitoring, and tracking QMS status changes. The QI tool is universal for all company departments regardless of their requirements, easy to use, and customisable by adding individual company-specific quality parameters. This makes the QI tool applicable not only to drug development departments but also to other research units

Physiological and Morphological Correlates of Presynaptic Inhibition in Primary Afferents of the Lamprey Spinal Cord

Patch-clamp recordings in a whole-cell mode were performed on dorsal sensory cells enzymatically isolated from the spinal cord of two lamprey species, Ichthyomyzon unicuspis and Lampetra fluviatilis. The voltage-activated currents through calcium channels were analysed. GABA and the specific GABAB receptor agonist baclofen reduced the peak amplitude of inward Ba2+ current, as a robust alternate charge carrier through voltage-dependent Ca2+ channels. These effects were dose-dependent and reversible. GABAB receptor antagonists, 2-hydroxysaclofen and δ-amino-n-valeric acid, blocked the reduction of Ba2+ currents by GABA and baclofen, while bicuculline, a GABAA receptor antagonist, had no blocking action. GABA and baclofen did not modify the dorsal sensory cell membrane conductance, indicating that they did not activate ligand-gated channels. However, GABA, but not baclofen, considerably increased membrane conductance and induced Cl- currents in isolated multipolar neurons (presumably interneurons and/or motoneurons). These findings suggest that GABA and baclofen action on lamprey dorsal sensory cells is mediated by GABAB receptors. We concluded that GABA-mediated presynaptic inhibition of lamprey dorsal sensory cell fibers results from GABAB receptor activation followed by a decrease of inward voltage-activated calcium currents. Appositions of GABA-immunoreactive boutons to horseradish peroxidase-labeled fibers from the dorsal root were observed at the ultrastructural level in the dorsal column using postembedding immunogold cytochemistry. It seems likely that these appositions represent the morphological substrate of dorsal sensory cell fiber presynaptic inhibition. In very rare cases, ultrastructural features were observed which could be interpreted as synaptic specializations between the GABA-immunoreactive boutons and the primary afferent fibers. The extrasynaptic action of GABA as a basis of presynaptic inhibition of this population of primary afferent neurons is discussed

Внедрение и оценка системы менеджмента качества в области фармацевтической разработки биотехнологических лекарственных препаратов

Scientific relevance. One of the challenges associated with the development of medicines lies in creating a quality management system (QMS) and tools to assess its performance.Aim. The study aimed to propose a QMS model for the development of biotechnology-derived medicinal products and a performance evaluation tool for this QMS universally applicable in the research centre of the company regardless of the specific activities of its individual divisions.Materials and methods. The study analysed internal audit outcomes, non-compliance responses, and the document flow of the research centre. Parameter values for quality index (QI) calculations were entered into validated Microsoft Excel spreadsheets. Data analysis and visualisation involved using the Microsoft Power Business Intelligence (BI) business analytics platform (mainly, the Power BI Desktop and Power BI Service components).Results. The QMS was implemented, and the authors proposed their QMS performance evaluation tool universally applicable to all the divisions of the research centre.Conclusions. The authors proposed their QMS model for the development of biotechnology-derived medicinal products and the QI tool for collecting digital data, carrying out standardised monitoring, and tracking QMS status changes. The QI tool is universal for all company departments regardless of their requirements, easy to use, and customisable by adding individual company-specific quality parameters. This makes the QI tool applicable not only to drug development departments but also to other research units.Актуальность. Важными задачами при создании лекарственных средств являются формирование в области фармацевтической разработки системы менеджмента качества (СМК) и разработка инструментов оценки ее эффективности.Цель. Предложить модель системы менеджмента качества в области фармацевтической разработки биотехнологических препаратов, а также разработать механизм оценки эффективности функционирования данной системы в различных подразделениях научно-исследовательского центра.Материалы и методы. В работе представлены данные о результатах внутренних аудитов научно-исследовательского центра, результаты работы с несоответствиями и данные о документообороте. Значения параметров для расчета индекса качества регистрировали в валидированных Excel-файлах. Для анализа и визуализации данных использовали программный продукт Microsoft Power BI (Business Intelligence), предназначенный для бизнес-анализа данных. Основными используемыми компонентами платформы Power BI являлись Power BI Desktop и Power BI Service.Результаты. Внедрена СМК и разработан механизм, позволяющий проводить оценку эффективности работы СМК в подразделениях научно-исследовательского центра вне зависимости от индивидуальных требований к каждому подразделению.Выводы. Предложены модель организации СМК в области фармацевтической разработки биотехнологических препаратов и инструмент (индекс качества) для сбора оцифрованных данных, проведения унифицированного мониторинга и отслеживания динамики изменения состояния СМК вне зависимости от индивидуальных требований к каждому подразделению. Отмечено, что индекс качества может быть применен не только в отношении подразделений разработки лекарственных препаратов, но и в отношении других научных подразделений ввиду его универсальности, простоты и возможности модификации за счет введения дополнительных индивидуальных показателей качества

Чтение как эффективный способ изучения английского языка

Целью данной работы было изучить, как чтение текстов на английском языке помогает ученикам любого уровня эффективно расширять словарный запас, изучать грамматические конструкции и повышать уровень владения иностранным языком для дальнейшей разработки универсальной и эффективной методики обучению английскому языку посредством чтения иностранных текстов.The purpose of this work was to study how reading texts in English helps students of any level to effectively expand their vocabulary, study grammatical constructions and improve their proficiency in a foreign language for further development of a universal and effective method for teaching English through reading foreign texts

Calcium Currents and GABA\u3csub\u3eB\u3c/sub\u3e Receptors in the Dorsal Sensory Cells of the Lamprey Spinal Cord

Patch-clamp studies were performed on the isolated dorsal sensory cells of the spinal cords of three species of lamprey,Ichthyomyzon unicuspis, Petromyzon marinus, andLampetra fluviatilis, to measure changes in the amplitudes of calcium current induced by GABA and its specific antagonists and agonists. The experiments showed that GABA (4 mM) reduced the peak amplitude of the calcium current by 28.5±4.9%, with subsequent recovery to 96.2±9.2% of control (n=45). The GABAB agonist baclofen had similar effects. The GABAA agonists glycine and taurine had no effect on the Ca2+ current. The inhibitory effect of GABA was blocked by 2-hydroxysaclofen (a GABAB antagonist), but persisted in the presence of bicuculline (a GABAA antagonist). These results are evidence that the membranes of dorsal sensory cells contain GABAB receptors, which significantly increases our under-standing of the mechanisms of presynaptic inhibition in the spinal cords of the cyclostomata

The Effects of Baclofen on Calcium Channel Currents in Dorsal Sensory Cells of the Spinal Cord in the Lamprey

Dorsal sensory cells isolated from the spinal cord of the lamprey speciesIchthyomyzon unicuspis andLampetra fluviatilis were used for whole-cell patch-clamp studies of the effects of baclofen on calcium channel currents, evoked in conditions in which Na+, K+ currents were blocked, by depolarizing membranes from constant holding potentials of −100 or −80 mV to +30 mV. Ba ions were used as carriers of currents through calcium channels. These studies demonstrated that baclofen (0.5 mM) decreased the peak amplitude of the Ba2+ current by an average of 22.5±4.2% (n=12) in dorsal sensory cells of the lampreyIchthyomyzon unicuspis and by 28.4±3.3% in the dorsal sensory cells ofLampetra fluviatilis (n=25). The conductivity of dorsal sensory cell membranes in the presence of baclofen (and GABA) did not change. The blocking action of baclofen persisted in the presence of bicuculline (100 μM) and was lifted by addition of δ-aminovaleric acid and 2-hydroxysaclofen to the perfusing solution. These results are interpreted as evidence for the presence of GABAB receptors in dorsal sensory cell membranes. The data were compared with published results, and the question of the functional significance of GABAB receptors in the dorsal sensory cells (primary afferent cells) of cyclostomata is discussed