Features of disease course of some forms of herpesvirus infection

Among the many factors that directly affect the immune system, infections caused by the herpes virus deserve special attention. In recent years, there has been an increase in the number of patients suffering from chronic recurrent herpesvirus infections, which in many cases are accompanied by severe general malaise and a number of therapeutic complaints. Today about 80-95% of the population is infected with Epstein-Barr virus EBV. Primary infection of EBV leads to lifelong persistence of the pathogen with possible periodic reactivation under the action of various immunosuppressive factors, which leads to chronic forms of the disease. EBV can cause chronic manifest and erased forms of the disease, running on the type of chronic mononucleosis. Thus, the study of the nature of clinical and laboratory changes in patients with chronic EBV infection and the peculiarities of the disease is an urgent problem of our time. The aim of this work was to study the features of the course of chronic EBV infection. We performed a comprehensive clinical and laboratory examination of 128 patients with chronic EBV infection (reactivation period). The diagnosis of chronic EBV infection was established on the basis of medical history, complaints, the presence of specific antibodies to EBV antigens and the detection of virus DNA in the blood. Studies of clinical blood tests of patients with chronic EBV infection and the control group showed differences. Thus, in patients with chronic EBV infection, a significantly elevated ESR level was found - 12.6 ± 1.8 versus 4.5 ± 1.1 inthe control group (p <0.05). The levels of erythrocytes, hemoglobin and platelets did not differ significantly from the control group. In patients with chronic EBV infection, there was a decrease in the level of leukocytes (5.13 ± 0.22) x 109/ l against (6.20 ± 1.8) x 109/l in the control group (p> 0.05), but these changes did not go beyond the norms established for healthy people. Analysis of the leukocyte formula revealed the presence of patients with chronic WEB infection with a significant increase in the relative content of lymphocytes - 42.50 ± 2.0% against 33.70 ± 2.68% (p <0.05) and monocytes - 11.15 ± 0.9% vs. 3.0 ± 0.6% (p <0.05), which was the basis for confirming the presence of a chronic infectious process. In general, 94.8% of patients with chronic EBV infection had lymphocytosis and 82.5% had monocytosis. The relative number of neutrophils in patients with chronic WEB infection averaged 45.35 ± 4.1% against 61.7 ± 3.8% in the control group (p <0.05); the relative number of eosinophils and basophils was within normal limits. When studying the absolute number of lymphocytes and monocytes in patients with chronic EBV infection, there was a tendency to increase them and their level was on average (2.13 ± 0.7) x 109/ l and (0.51 ± 0.08) x 109/ l against (2.39 ± 0.7) x 109/ l and (0.47 ± 0.08) x 109/ l in the control group (p> 0.05). The absolute number of neutrophils in patients with chronic WEB infection was significantly lower than in the control group and was 2.70 x 109/ l versus 4.21 x 109/ l (p <0.05). The study of the absolute number of eosinophils and basophils in patients with chronic EBV infection did not reveal statistically significant differences compared with the control group. Thus, according to the results of our study, we found that patients with chronic EBV infection among the clinical manifestations were most often chronic tonsillitis, chronic fatigue syndrome and peripheral lymphadenopathy. Analysis of the results of laboratory studies revealed a significant increase in ESR, relative lymphocytes and monocytes, as well as a decrease in absolute and relative neutrophils, compared with the control group of patients, indicating a shift in white blood cell count, characteristic of the viral etiology of the disease what associated with the EBV.DOI: 10.5281/zenodo.403896

The changes in the amino and fatty acid profiles in the semifinished foodstuffs based on broiler meat and components of chicken eggs after different types of thermal treatment

The changes in the amino and fatty acid profiles in the semifinished foodstuffs (SFFs) based on broiler meat and coagulated chicken egg melange after different types of thermal treatment (water or steam boiling, braising, baking, frying) were studied. The amino acid profiles were determined on Knauer analyzer; tryptophan by standard method. The biological value of the treated products was assessed using amino acid balance coefficients calculated by the method of N. N. Lipatov. It was found that the changes in the initial amino acid profiles of the SFFs were the least after water and steam boiling; braising and baking were found to increase the contents of the essential amino acids. The amino acid profiles in the treated SFFs were close to the reference values. The best criteria of their biological value (coefficient of rationality of amino acid composition, comparable redundance) were found after water and steam boiling. It was found that all types of thermal treatments insignificantly affected the parameters of fatty acid balance within the SFFs; the changes found were primarily related to slight increase in total content of saturated fatty acids and increase in total content of polyunsaturated fatty acids (PUFAs) in compare to initial profiles, by 2.64–3.88% depending on the treatment type. The changes in ω‑6/ω‑3 PUFAs ratios were more substantial especially after braisin

Association study of genetic polymorphisms of vasoactive hormones with the risk of preeclampsia

The present study was designed to investigate an association of genetic polymorphisms of vasoactive hormones with the risk of preeclampsi

The VNTR polymorphism of the endothelial nitric oxide synthase gene and blood pressure in women at the end of pregnancy

Examine the association of the 4a/4b polymorphism of endothelial nitric oxide synthase (eNOS) with blood pressure in women at late pregnanc

Analysis of Involvement of Cytokine Genetic Polymorphisms in Development of Genital Endometriosis

The article outlines the role of cytokines in development of genital endometriosis. There were detected associations between genetic polymorphisms and their combinations and development of genital endometriosis among women in the Central region of Russi

Insilico test of functional role of rs8068318 polymorphism of arterial hypertension-associated TBX2 candidate gene

In the course of this study, the functional role of rs8068318 polymorphism of the TBX2 candidate gene associated with the development of arterial hypertension was studied. The selection of the polymorphic locus was based on the data of the catalog of genome-wide association study (GWAS) of the National Human Genome Research Institute. The functional role was evaluated using online software: HaploReg (v4.1), GTExportal, and PolyPhen-

Candidate genes for age at menarche are associated with endometriosis

Sixteen candidate genes for age at menarche were reported to be associated with endometriosis. The observed pleiotropy of these loci suggests a shared genetic architecture for the two phenotype

Dataset of allele and genotype frequencies of five polymorphisms candidate genes analyzed for association with body mass index in Russian women

Data on the allele and genotype frequencies of the five single nucleotide polymorphisms (SNPs) 5 genes - rs1514175 TNNI3K, rs713586 RBJ, rs887912 FANCL, rs2241423 MAP2K5, rs12444979 GPRC5B in Russian women are presented. Several genome-wide association studies identified these SNPs could be significant genetic markers associated with body mass index (BMI