Phylogenetic analyses of typical bovine rotavirus genotypes G6, G10, P[5] and P[11] circulating in Argentinean beef and dairy herds

Group A rotavirus (RVA) is one of the main causes of neonatal calf diarrhea worldwide. RVA strains affecting Argentinean cattle mainly possess combinations of the G6, G10, P[5] and P[11] genotypes. To determine RVA diversity among Argentinean cattle, representative bovine RVA strains detected in diarrheic calves were selected from a survey conducted during 1997–2009. The survey covered the main livestock regions of the country from dairy and beef herds. Different phylogenetic approaches were used to investigate the genetic evolution of RVA strains belonging to the prevalent genotypes. The nucleotide phylogenetic tree showed that all genotypes studied could be divided into several lineages. Argentinean bovine RVA strains were distributed across multiple lineages and most of them were distinct from the lineage containing the vaccine strains. Only the aminoacid phylogenetic tree of G6 RVA strains maintained the same lineages as observed at the nucleotide level, whereas a different clustering pattern was observed for the aminoacid phylogenetic trees of G10, P[5] and P[11] suggesting that the strains are more closely related at the aminoacid level than G6 strains. Association between P[5] and G6(IV), prevalent in beef herd, and between P[11] and G6(III) or G10 (VI and V), prevalent in dairy herds, were found. In addition, Argentinean G6(III), G10, P[5] and P[11] bovine RVA strains grouped together with human strains, highlighting their potential for zoonotic transmission. Phylogenetic studies of RVA circulating in animals raised for consumption and in close contact with humans, such as cattle, contribute to a better understanding of the epidemiology of the RVA infection and evolution.Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garaicoechea, Lorena Laura. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Matthijnssens, J.. University of Leuven. Rega Institute for Medical Research; BélgicaFil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Área de Investigación en Producción y Sanidad Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Área de Investigación en Producción y Sanidad Animal; ArgentinaFil: Bilbao, Gladys Noemí. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Fernandez, Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Parra, G. I.. National Institutes of Health; Estados UnidosFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Diffusion as mixing mechanism in granular materials

We present several numerical results on granular mixtures. In particular, we examine the efficiency of diffusion as a mixing mechanism in these systems. The collisions are inelastic and to compensate the energy loss, we thermalize the grains by adding a random force. Starting with a segregated system, we show that uniform agitation (heating) leads to a uniform mixture of grains of different sizes. We define a characteristic mixing time, $\tau_{mix}$, and study theoretically and numerically its dependence on other parameters like the density. We examine a model for bidisperse systems for which we can calculate some physical quantities. We also examine the effect of a temperature gradient and demonstrate the appearance of an expected segregation.Comment: 15 eps figures, include

Velocity correlations in granular materials

A system of inelastic hard disks in a thin pipe capped by hot walls is studied with the aim of investigating velocity correlations between particles. Two effects lead to such correlations: inelastic collisions help to build localized correlations, while momentum conservation and diffusion produce long ranged correlations. In the quasi-elastic limit, the velocity correlation is weak, but it is still important since it is of the same order as the deviation from uniformity. For system with stronger inelasticity, the pipe contains a clump of particles in highly correlated motion. A theory with empirical parameters is developed. This theory is composed of equations similar to the usual hydrodynamic laws of conservation of particles, energy, and momentum. Numerical results show that the theory describes the dynamics satisfactorily in the quasi-elastic limit, however only qualitatively for stronger inelasticity.Comment: 12 pages (REVTeX), 15 figures (Postscript). submitted to Phys. Rev.

Granular flow down a rough inclined plane: transition between thin and thick piles

The rheology of granular particles in an inclined plane geometry is studied using molecular dynamics simulations. The flow--no-flow boundary is determined for piles of varying heights over a range of inclination angles $\theta$. Three angles determine the phase diagram: $\theta_{r}$, the angle of repose, is the angle at which a flowing system comes to rest; $\theta_{m}$, the maximum angle of stability, is the inclination required to induce flow in a static system; and $\theta_{max}$ is the maximum angle for which stable, steady state flow is observed. In the stable flow region $\theta_{r}<\theta<\theta_{max}$, three flow regimes can be distinguished that depend on how close $\theta$ is to $\theta_{r}$: i) $\theta>>\theta_{r}$: Bagnold rheology, characterized by a mean particle velocity $v_{x}$ in the direction of flow that scales as $v_{x}\propto h^{3/2}$, for a pile of height $h$, ii) $\theta\gtrsim\theta_{r}$: the slow flow regime, characterized by a linear velocity profile with depth, and iii) $\theta\approx\theta_{r}$: avalanche flow characterized by a slow underlying creep motion combined with occasional free surface events and large energy fluctuations. We also probe the physics of the initiation and cessation of flow. The results are compared to several recent experimental studies on chute flows and suggest that differences between measured velocity profiles in these experiments may simply be a consequence of how far the system is from jamming.Comment: 19 pages, 14 figs, submitted to Physics of Fluid

Simulation for the oblique impact of a lattice system

The oblique collision between an elastic disk and an elastic wall is numerically studied. We investigate the dependency of the tangential coefficient of restitution on the incident angle of impact. From the results of simulation, our model reproduces experimental results and can be explained by a phenomenological theory of the oblique impact.Comment: 30 pages, 9 figures, submitted to J. Phys. Soc. Japa

Extended kinetic theory applied to inclined granular flows: role of boundaries

We compare the predictions of extended kinetic theory (EKT), where the roles of surface friction and correlation in fluctuation velocities are taken into account, with discrete element simulations of steady, fully-developed, inclined flows of identical spheres over bumpy bases, in the presence and absence of flat, frictional sidewalls. We show that the constitutive relation for the pressure of EKT must be modified in the proximity of the boundary, because of the influence of excluded volume and shielding associated with collisions of particles with the boundary itself. We also note that currently available boundary conditions for flows over bumpy planes in kinetic theory underestimate the energy dissipation. These two observations explain the lack of agreement of EKT with the simulations, in terms of the maximum angles of inclination for which steady, fully-developed flows are possible. That is, for some high angles of inclination, EKT does not have solutions, while steady flows are predicted in DEM. However, whenever a solution to the system of differential equations of EKT does exist, the predicted distributions of velocity, solid volume fraction and granular temperature satisfactorily match the numerical measurements. The incompressible, algebraic approximation of EKT, which ignores the conduction of energy in the energy balance, admits solutions for a wider range of angles of inclination, as in the simulations, but fails to reproduce the quantitative and qualitative behaviour of solid volume fraction and granular temperature in the two conductive layers at the top and bottom of the flow. When frictional sidewalls are added to the domain, we show that the spanwise ratio of shear stress to pressure is linearly distributed in the dense core region of the flow, confirming that the sidewalls exert, on average, a Coulomb-like resistance to the flow with an effective friction coefficient which is less than half the actual particle-wall friction

Geolocation and stable isotopes indicate habitat segregation between sexes in Magellanic penguins during the winter dispersion

The Magellanic penguin Spheniscus magellanicus is a top predator and a major consumer of marine resources in the Patagonian Continental Shelf and worldwide. It is the most migratory of the Spheniscus penguins, but until recently, its migration route was only partially known. Our aims with regard to the Magellanic penguin breeding on Isla Quiroga, Argentina, were: 1) to compare the distribution during the winter period between sexes and 2) in case that habitat is segregated, to evaluate possible proximate causes like competitive exclusion and/or habitat specialization. In March 2017 and 2018, prior to migration, we equipped 26 penguins with MK3/4 geolocators, which were recovered in late September/early October. Penguins dispersed northwards up to 29°S and southwards up to 56°S, near the Beagle Channel, being distributed over 2158 ± 50 km of latitudinal range and showing a large-scale distribution pattern across longitudes in waters within the continental shelf. We found a longitudinal segregation between sexes in their winter grounds, with males closer to the 200-m isobath and further from the coastline than females. Morphological and physiological differences between sexes might allow males to dive deeper at a lower cost than females, which could result in a more offshore path for males. This spatial segregation was supported by bulk blood stable isotope values and estimated isotopic niche, which differed between males and females at their arrival from winter breeding grounds, but not while penguin breed and behave as central place foragers in the colony. For the first time in this species, we combined two independent tools, geolocation and stable isotope data, and find that sexual segregation in habitat use could potentially generate an optimal winter foraging strategy for both sexes, precluding potential intersex competition for food.Fil: Barrionuevo, Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Ciancio Blanc, Javier Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; ArgentinaFil: Steinfurth, Antje. Royal Society For The Protection Of Birds; Reino UnidoFil: Frere, Esteban. Universidad Nacional de la Patagonia Austral; Argentin

Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010

Bovine coronavirus (BCoV) is an important viral pathogen associated with neonatal calf diarrhea. Our aim was to investigate the incidence of BCoV in diarrhea outbreaks in beef and dairy herds from Argentina during 1994–2010. A total of 5.365 fecal samples from diarrheic calves were screened for BCoV diagnosis by ELISA. The virus was detected in 1.71% (92/5365) of the samples corresponding to 5.95% (63/1058) of the diarrhea cases in 239 beef and 324 dairy farms. The detection rate of BCoV was significantly higher in dairy than in beef herds: 12.13% (29/239) vs. 4.32% (14/324) respectively. Phylogenetic analysis of the hypervariable S1 region of seven representative samples (from different husbandry systems, farm locations and years of sampling) indicated that BCoV strains circulating in Argentinean beef and dairy herds formed a cluster distinct from other geographical regions. Interestingly, Argentinean strains are distantly related (at both the nucleotide and amino acid levels) with the Mebus historic reference BCoV strain included in the vaccines currently available in Argentina. However, Mebus-induced antibodies were capable of neutralizing the BCoV Arg95, a field strain adapted to grow in vitro, and vice versa, indicating that both strains belong to the same CoV serotype reported in cattle. This work represents the first large survey describing BCoV circulation in Argentinean cattleInstituto de VirologíaFil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Chinese Academy of Agriculture Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; ChinaFil: Rodriguez, Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Nuñes, I. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; BrasilFil: Souza, Sibele P. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; BrasilFil: Bilbao, G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Sanidad Animal; ArgentinaFil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional del Centro de la provincia de Buenos Aires. Facultad Ciencias Veterinarias; ArgentinaFil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Odeon, Anselmo Carlos. Estación Experimental Agropecuaria Balcarce. Laboratorio de Sanidad Animal; ArgentinaFil: Saif, LInda J. Ohio State University. Food Animal Health Research Program; Estados UnidosFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentin