ПРИНЦИПИАЛЬНОЕ РАЗЛИЧИЕ МЕТОДОВ НИЗКОЧАСТОТНОГО И УЛЬТРАЗВУКОВОГО ВОЗДЕЙСТВИЙ НА РАСПЛАВЫ

There were considered factors that determine difference of low and ultrasonic frequency treatment methods with regard to composite melts: cavitation events, dissipation losses, capability to turbulent mixing of melt. There was proven independence of every of these methods.Рассматриваются факторы, определяющие различие низкочастотного и ультразвукового методов обработки композиционных расплавов: кавитационные явления, диссипативные потери, способность к турбулентному перемешиванию расплава. Доказывается самостоятельность каждого из методов

OPTIMUM NOMINAL METHOD MODIFICATION AT THE MANAGEMENT OF MOVING OBJECTS UNDER UNCERTAINTY

ABSTRACT The articleis devoted to the development of models and information support for the search of the input parameters providing effective functioning of a moving object in the incompleteness data conditions. The problem of optimization is solved by theapplication of fuzzy intervals and variables. Parameters of moving objects of autonomous navigation and function of optimization are considered in the form of linguistic and fuzzy variables. Experts set functions of the accessory of fuzzy variables. The concept of the function of the accessory of deviations from the set face value of input parameter is defined. Modification of the method of the optimum of face value is considered at management of moving objects in the conditions of uncertainty. The algorithm and information support is developed for search of the fuzzy maximum of the function of the productivity of autonomous navigation at management of moving objects. The description of work with information support is provided

Interplay of unsteady aerodynamics and flight dynamics of transport aircraft in icing conditions

Airframe icing causes significant degradation of aerodynamic characteristics and influences the flight safety. Wind tunnel study of longitudinal steady and unsteady aerodynamic characteristics of a transport aircraft in icing conditions is carried out in order to develop mathematical model of aerodynamics in the extended flight envelope. The wind tunnel results are validated through flight tests conducted for the real aircraft. Large, glaze-horn ice shapes, corresponding to holding flight phase, are considered. Influence of an ice protection system as well as its failure is examined. Effect of icing on the unsteady aerodynamics characteristics is studied not only through wind tunnel tests but also via analysis of subsequent influence on the flight dynamics of the aircraft. The conducted study shows that the ice shapes of the holding phase leads to reduced stall angle of attack (AoA), maximum lift, and longitudinal damping. Flight dynamics analysis demonstrates that dangerous aircraft behaviour in the form of high AoA departure and limit cycle oscillations (LCO) can be observed at smaller elevator deflections for the iced aircraft. Taking into account icing influence on the unsteady aerodynamics in the flight dynamics simulations revealed degradation of the dynamic response and deterioration of phase portraits of the system. Even for small AoA and elevator deflection the aircraft might be trapped into the basin of attraction of high-AoA LCO. In addition, incorporating icing effects in unsteady aerodynamics manifest larger amplitude of LCO

Современные технологии в подготовке специалистов по клинической лабораторной диагностике в одесском национальном медицинском университете

Підготовка фахівців з клінічної лабораторної діагностики на сучасному рівні потребує втілення новітніх технологій у практичну складову навчання, як на до -, так і післядипломному етапах. Цьому сприяє нова міжкафедральна клінічна лабораторія ОНМедУ, яка надає можливість навчання на сучасному обладнанні та дозволяє засвоїти сучасні технології лікарям-інтернам, підвищує інтерес до проходження спеціалізації і підвищення кваліфікації на базі ОНМедУ слухачівлаборантів циклів підвищення кваліфікації.Training specialists in clinical laboratory diagnostics at the present stage involves the introduction of new technologies in the practical component of training at the pre- and postgraduate stages. This is facilitated by the new interdisciplinary clinical laboratory of ONMedU, which gives the opportunity to study the programme on modern equipment and allows students and interns to master the latest technologies, increases interest in specialization and advanced training in ONMED among laboratory doctors.Подготовка специалистов по клинической лабораторной диагностике на современном этапе предполагает внедрение новейших технологий в практическую составляющую обучения на до- и последипломном этапах. Этому способствует новая межкафедральная клиническая лаборатория ОНМедУ, дающая возможность обучения на современном оборудовании и позволяющая усвоить новейшие технологии студентам, врачам - интернам, повышает интерес к прохождению специализации и повышения квалификации в ОНМедУ у врачейлаборантов

Filovirus RefSeq Entries: Evaluation and Selection of Filovirus Type Variants, Type Sequences, and Names

Sequence determination of complete or coding-complete genomes of viruses is becoming common practice for supporting the work of epidemiologists, ecologists, virologists, and taxonomists. Sequencing duration and costs are rapidly decreasing, sequencing hardware is under modification for use by non-experts, and software is constantly being improved to simplify sequence data management and analysis. Thus, analysis of virus disease outbreaks on the molecular level is now feasible, including characterization of the evolution of individual virus populations in single patients over time. The increasing accumulation of sequencing data creates a management problem for the curators of commonly used sequence databases and an entry retrieval problem for end users. Therefore, utilizing the data to their fullest potential will require setting nomenclature and annotation standards for virus isolates and associated genomic sequences. The National Center for Biotechnology Information’s (NCBI’s) RefSeq is a non-redundant, curated database for reference (or type) nucleotide sequence records that supplies source data to numerous other databases. Building on recently proposed templates for filovirus variant naming [ ()////-], we report consensus decisions from a majority of past and currently active filovirus experts on the eight filovirus type variants and isolates to be represented in RefSeq, their final designations, and their associated sequences

Virus nomenclature below the species level : a standardized nomenclature for filovirus strains and variants rescued from cDNA

Specific alterations (mutations, deletions, insertions) of virus genomes are crucial for the functional characterization of their regulatory elements and their expression products, as well as a prerequisite for the creation of attenuated viruses that could serve as vaccine candidates. Virus genome tailoring can be performed either by using traditionally cloned genomes as starting materials, followed by site-directed mutagenesis, or by de novo synthesis of modified virus genomes or parts thereof. A systematic nomenclature for such recombinant viruses is necessary to set them apart from wild-type and laboratoryadapted viruses, and to improve communication and collaborations among researchers who may want to use recombinant viruses or create novel viruses based on them. A large group of filovirus experts has recently proposed nomenclatures for natural and laboratory animal-adapted filoviruses that aim to simplify the retrieval of sequence data from electronic databases. Here, this work is extended to include nomenclature for filoviruses obtained in the laboratory via reverse genetics systems. The previously developed template for natural filovirus genetic variant naming,\virus name[(\strain[/)\isolation host-suffix[/ \country of sampling[/\year of sampling[/\genetic variant designation[-\isolate designation[, is retained, but we propose to adapt the type of information added to each field for cDNA clone-derived filoviruses. For instance, the full-length designation of an Ebola virus Kikwit variant rescued from a plasmid developed at the US Centers for Disease Control and Prevention could be akin to ‘‘Ebola virus H.sapiens-rec/COD/1995/Kikwit-abc1’’ (with the suffix ‘‘rec’’ identifying the recombinant nature of the virus and ‘‘abc1’’ being a placeholder for any meaningful isolate designator). Such a full-length designation should be used in databases and the methods section of publications. Shortened designations (such as ‘‘EBOV H.sap/COD/95/ Kik-abc1’’) and abbreviations (such as ‘‘EBOV/Kik-abc1’’) could be used in the remainder of the text, depending on how critical it is to convey information contained in the full-length name. ‘‘EBOV’’ would suffice if only one EBOV strain/variant/isolate is addressed.http://link.springer.com/journal/705hb201

Virus nomenclature below the species level : a standardized nomenclature for laboratory animal-adapted strains and variants of viruses assigned to the family Filoviridae

The International Committee on Taxonomy of Viruses (ICTV) organizes the classification of viruses into taxa, but is not responsible for the nomenclature for taxa members. International experts groups, such as the ICTV Study Groups, recommend the classification and naming of viruses and their strains, variants, and isolates. The ICTV Filoviridae Study Group has recently introduced an updated classification and nomenclature for filoviruses. Subsequently, and together with numerous other filovirus experts, a consistent nomenclature for their natural genetic variants and isolates was developed that aims at simplifying the retrieval of sequence data from electronic databases. This is a first important step toward a viral genome annotation standard as sought by the US National Center for Biotechnology Information (NCBI). Here, this work is extended to include filoviruses obtained in the laboratory by artificial selection through passage in laboratory hosts. The previously developed template for natural filovirus genetic variant naming ( //<year of sampling>/-) is retained, but it is proposed to adapt the type of information added to each field for laboratory animal-adapted variants. For instance, the full-length designation of an Ebola virus Mayinga variant adapted at the State Research Center for Virology and Biotechnology “Vector” to cause disease in guinea pigs after seven passages would be akin to “Ebola virus VECTOR/C.porcellus-lab/COD/1976/Mayinga- GPA-P7”. As was proposed for the names of natural filovirus variants, we suggest using the fulllength designation in databases, as well as in the method section of publications. Shortened designations (such as “EBOV VECTOR/C.por/COD/76/May-GPA-P7”) and abbreviations (such as “EBOV/May-GPA-P7”) could be used in the remainder of the text depending on how critical it is to convey information contained in the full-length name. “EBOV” would suffice if only one EBOV strain/variant/isolate is addressed.This work was funded in part by the Joint Science and Technology Office for Chem Bio Defense (proposal #TMTI0048_09_RD_T to SB).http://www.springerlink.com/content/0304-8608/hb2013ab201

Bacterial communities associated with atherosclerotic plaques from Russian individuals with atherosclerosis

© 2016 Ziganshina et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Atherosclerosis is considered a chronic disease of the arterial wall and is the major cause of severe disease and death among individuals all over the world. Some recent studies have established the presence of bacteria in atherosclerotic plaque samples and suggested their possible contribution to the development of cardiovascular disease. The main objective of this preliminary pilot study was to better understand the bacterial diversity and abundance in human atherosclerotic plaques derived from common carotid arteries of individuals with atherosclerosis (Russian nationwide group) and contribute towards the further identification of a main group of atherosclerotic plaque bacteria by 454 pyrosequencing their 16S ribosomal RNA (16S rRNA) genes. The applied approach enabled the detection of bacterial DNA in all atherosclerotic plaques. We found that distinct members of the order Burkholderiales were present at high levels in all atherosclerotic plaques obtained from patients with atherosclerosis with the genus Curvibacter being predominant in all plaque samples. Moreover, unclassified Burkholderiales as well as members of the genera Propionibacterium and Ralstonia were typically the most significant taxa for all atherosclerotic plaques. Other genera such as Burkholderia, Corynebacterium and Sediminibacterium as well as unclassified Comamonadaceae, Oxalobacteraceae, Rhodospirillaceae, Bradyrhizobiaceae and Burkholderiaceae were always found but at low relative abundances of the total 16S rRNA gene population derived from all samples. Also, we found that some bacteria found in plaque samples correlated with some clinical parameters, including total cholesterol, alanine aminotransferase and fibrinogen levels. Finally, our study indicates that some bacterial agents at least partially may be involved in affecting the development of cardiovascular disease through different mechanisms

Speech by Deputy-Minister HB Holomisa : Local Agenda 21 African Regional Seminar; ESKOM Conference Centre, 10 APRIL 1995

© 2016 Ziganshina et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Atherosclerosis is considered a chronic disease of the arterial wall and is the major cause of severe disease and death among individuals all over the world. Some recent studies have established the presence of bacteria in atherosclerotic plaque samples and suggested their possible contribution to the development of cardiovascular disease. The main objective of this preliminary pilot study was to better understand the bacterial diversity and abundance in human atherosclerotic plaques derived from common carotid arteries of individuals with atherosclerosis (Russian nationwide group) and contribute towards the further identification of a main group of atherosclerotic plaque bacteria by 454 pyrosequencing their 16S ribosomal RNA (16S rRNA) genes. The applied approach enabled the detection of bacterial DNA in all atherosclerotic plaques. We found that distinct members of the order Burkholderiales were present at high levels in all atherosclerotic plaques obtained from patients with atherosclerosis with the genus Curvibacter being predominant in all plaque samples. Moreover, unclassified Burkholderiales as well as members of the genera Propionibacterium and Ralstonia were typically the most significant taxa for all atherosclerotic plaques. Other genera such as Burkholderia, Corynebacterium and Sediminibacterium as well as unclassified Comamonadaceae, Oxalobacteraceae, Rhodospirillaceae, Bradyrhizobiaceae and Burkholderiaceae were always found but at low relative abundances of the total 16S rRNA gene population derived from all samples. Also, we found that some bacteria found in plaque samples correlated with some clinical parameters, including total cholesterol, alanine aminotransferase and fibrinogen levels. Finally, our study indicates that some bacterial agents at least partially may be involved in affecting the development of cardiovascular disease through different mechanisms