Morphological features of bovine placenta in case of viral, bacterial and protozoal infections

The problem of the intrauterine  infection of fetus is one of the most critical ones in veterinary obstetrics and in perinatology due to the high level of infection in pregnant  cows, the risk of developmental disorder of fetus and the birth of sick calves. Complications of pregnancy occur in case of viral, bacterial and protozoal infections, when the pathogen  enters the uterus in an ascending or descending way with further transplacental  infection of the fetus. Morphological studies of placenta  of Black Pied cattle infected with bovine viral diarrhea, chlamydia and neosporosis were carried out. The presence of the pathogen was confirmed by serological and molecular genetic methods. The material used for histological studies was the fetal part of placenta. After sampling, the material was fixed in a 10% neutral formalin solution, then xylene-free method  for histological preparation  was used. Afterwards, samples were embedded in paraffin. In order to study morphological structures, samples were sectioned at 5–6 µm, and stained with hematoxylin and eosin. Histological sections were analyzed using a Leica DM 1000 light microscope at a magnification of 100×,  200×,  400×,  630×.  On the basis of the conducted studies, it was established that bovine viral diarrhea-associated morphofunctional changes in the “mother – placenta – fetus” system are characterized by involutive-dystrophic changes with microcirculation disorders and the development of an immunity-associated inflammatory process. Chlamydia abortus intrauterine  infection in the “mother – placenta – fetus” system in cows causes a complex of destructive morphological and functional changes of an infectious and toxic nature with a pronounced inflammatory reaction, involvement of blood vessels in the pathological process, and endothelial dysfunction development,  alongside with tissue necrosis in case of a chronic process. The presence of cellular structures in the placenta and the inner part of the umbilical cord is a pathognomonic sign of chlamydia. The role of transplacental transmission of Neospora caninum in cattle was confirmed, the Neospora parasites subjected to basophilic staining were detected not only in the tissues of the placenta, but also in histological sections of the fetus heart and liver. The main characteristic diagnostic sign is the presence of basophilic stained Neospora parasites  in the organs of the mother  and fetus, placenta, and intervillous space. As part of the study morphological features of placenta, one of the most unique histohematic barriers and the basic element of the intrauterine infectious process, were determined

GENETIC FORMATION FACTORS OF DAIRY EFFICIENCY AND QUALITY OF CATTLE MILK

Experimental results of studying the efficiency of cattle selection method use on a genotype are presented to technologies of industrial milk production. For carrying out researches three groups of cows of the Ural black-andwhite breed type from lines Sovering Reflexion, Vis Back Ideal, Montwick Chiftain were created, up to 20 heads in every. The comparative analysis of dairy efficiency and quality of cow milk of different linear accessory is carried out. Impact of linear accessory of cows on a milk yield in 305 days of lactation, the content in milk of fat and protein, a ratio in milk protein of replaceable and irreplaceable amino acids is defined. It is revealed that the Ballele a kappa casein is characterized by the high content of protein in milk with the best abomasal coagulability. 14.3% of cows of the line Montwick Chiftain and 8% Sovering Reflexion had a desirable genotype on kappa casein - Centuries. Protein content in cow milk with a genotype of BB exceeded milk-protein indicators of cows of other genotypes by 0.14-0.18%. By amount of irreplaceable amino acids cow milk of the line Vis Back Ideal was the best that exceeded value of this indicator in cow milk of the line Montwick Chiftain and Sovering Reflexion by 9.5% and 4.5% respectively. It is established that in cow milk with AA genotype the share of irreplaceable amino acids exceeded their content as a part of the general protein in cow milk with a genotype of BB and AB for 8.9 and 39% respectively. Keywords: milk, kappa casein, milk fat and protein, selection, genotype, amino acids

The use of nanosecond electron beam for the eggs surface disinfection in industrial poultry

Development of the technology bases of egg disinfection using surface irradiation is executed by the nanosecond electron beam. Experiments on shell eggs irradiation using the frequency nanosecond URT-0.5 accelerator were made. Determination of absorbed dose distributionwas executed. In case of irradiation by the electron beam with absorbed dose level 5 kGy, it is enough for the full disinfection of the surface and pores of egg shell. The absorbed dose in the egg at the expense of bremsstrahlung will not exceed 0.08 Gy which can't have essential action on protein. Irradiation of shell eggs batch in plastic package of 100 pieces pledged in an incubator together with an inspection lot of not irradiated egg was made. The percent of eggs deductibility and chickens survival of pilot and control batches were identical, indicating that there was no significant radio biological effect from irradiation of egg internal structures. The received chickens were grown up within the 5 weeks. It is established that the chickens who are grown up from the irradiated egg have no essential differences in development. © Published under licence by IOP Publishing Ltd.This work was performed within a support of the Russian Science No. 16-16-04038)

Clinical and morphological features of salmonellosis in cows and calves affected by heat stress

Exposure to abnormally high temperatures in the Middle Urals has ledtochronic heat stress in cattle. Despitethe measures taken toprotect thedairy herdfrom overheating (fans, irrigationwith coolwater, livestocksunshades), the heatstressunderminedtheimmunityofanimals, thus, leadingtoan excessiverisein Salmonella population in the gastrointestinal tract, increasedin test in alpermeability, and could also bethecause of increasedvirulence of such serotypes as – Salmonella choleraesuis, that are not typical for cattle. The simultaneous presenceof twopathogensof Salmonella entericasubsp. entericaspecies: i.e. Salmonella enteritidis and Salmonella choleraesuis – causeda number of clinical and morphological changesin adultcows. In cows, the infection manifested itself by persistent diarrhea with mucus, reduced milkproduction and intoxication. In calves, the disease was accompanied by septic signs, severein toxication, hyperthermia (40.5–41.0 °C), severed ehydration (morethan 7%) and death. Histopathological examination of bovineparenchymal organs revealed changes typical for salmonellos is pathological process: specific salmonella granulomas were foundin parenchymalorgans (liver, spleen). Some pathological processestypical for sepsiswere foundin other parenchymalorgans (kidneys, heart). The nature of pathological changes in dicates that there is as epticprocess that spreads both hematogenically and lymphogenically, and the structure of granulomas most likely indicates that such apathogen as Salmonelacholeraesuis circulates in cattle

Влияние теплового стресса на коров в сухостойный и послеродовой период

In the article, the authors presented information on the effect of heat stress on cows in the dry and postpartum periods. The study aims to study clinical and metabolic manifestations of heat stress in dry and fresh cows. The object of the study is cows of the early and late dry periods and new calves. The authors assessed the parameters of the microclimate of livestock premises, clinical examination of cattle, and blood sampling for hematological and biochemical studies. The authors also calculated the leukocyte index of intoxication, the shift index of blood leukocytes, and the lymphocytic index. Animals were found to have worsened general conditions, weakness, and reduced feed intake during the medical examination. An increase in body temperature (39.42±0.55 °C), tachypnea (86.45±8.04 resp.), and tachycardia (127.65±3.66 bpm) were also revealed. A hematological study revealed a decrease in the absolute number of lymphocytes and a shift in the indicators of the leukocyte formula. According to the results of the assessment of the lymphocytic index in cows during the dry period, the ratio of humoral and cellular immunity was balanced, and in new calves, an imbalance was observed. Biochemical screening of the blood serum of cows under conditions of heat stress showed significant changes in the metabolic profile against the background of a general decrease in feed intake. The content of total protein and globulins in the blood serum in the first days after calving was 68.36±3.48 (p≤0.01) and 35.36±3.89 g/l (p≤0.01), which is 16 .0 and 33.0% lower than in cows of the early dry period. Glucose and cholesterol levels progressively decreased after starting, reaching a minimum in the postpartum period (glucose - 3.14±0.59, total cholesterol - 2.18±0.55 mmol/l (p≤0.01)). The content of urea in the blood of animals increased significantly, reaching a maximum in the first days after calving - 7.44±1.48 mmol/l (p≤0.01). The authors also found an increase in the content of total bilirubin, aspartate aminotransferase, and creatine phosphokinase. Thus, heat stress leads to changes in the clinical and metabolic status of highly productive cows.Представлена информация о влиянии теплового стресса на коров в сухостойный и послеродовой период. Цель исследования – изучить клинико-метаболические проявления теплового стресса у сухостойных и новотельных коров. Объект исследования – коровы раннего, позднего сухостойного периода и новотельные. Проводили оценку параметров микроклимата животноводческих помещений, диспансеризацию крупного рогатого скота и отбор проб крови для гематологического и биохимического исследований. Рассчитывали также лейкоцитарный индекс интоксикации, индекс сдвига лейкоцитов крови и лимфоцитарный индекс. В ходе диспансеризации у животных было установлено ухудшение общего состояния, слабость, снижение потребления корма. Выявлено повышение температуры тела (39,42±0,55 оС), тахипноэ (86,45±8,04 дых. дв.) и тахикардия (127,65±3,66 уд/мин). При гематологическом исследовании установлено снижение абсолютного количества лимфоцитов и смещение показателей лейкоцитарной формулы. По результатам оценки лимфоцитарного индекса у коров сухостойного периода соотношение гуморального и клеточного звеньев иммунитета имело сбалансированный характер, а у новотельных наблюдался дисбаланс. Биохимический скрининг сыворотки крови коров в условиях теплового стресса показал достоверные изменения метаболического профиля на фоне общего снижения потребления корма. Содержание общего белка и глобулинов в сыворотке крови в первые дни после отела составило 68,36±3,48 (p≤0,01) и 35,36±3,89 г/л (p≤0,01), что на 16,0 и 33,0 % ниже, чем у коров раннего сухостойного периода. Уровень глюкозы и холестерина прогрессивно снижался после запуска, достигая минимума в послеродовой период (глюкоза – 3,14±0,59, общий холестерин – 2,18±0,55 ммоль/л (p≤0,01)). Содержание мочевины в крови животных достоверно увеличивалось, достигая максимума в первые дни после отела – 7,44±1,48 ммоль/л (p≤0,01). Установлено также повышение содержания общего билирубина, аспартатаминотрансферазы и креатинфосфокиназы. Таким образом, тепловой стресс приводит к изменениям клинико-метаболического статуса у высокопродуктивных коров

Anglicization of the French Language and Gallicization of the English Language at the Level of a Phraseological System

This article is a continuation of the authors’ research, the first results of which were published in the article “Pride and Prejudice”: the Anglicization of the French Language and the Gallicization of the English Language (2018). This time, the vector of consideration of two historically interdependent phenomena, the interactional nature of which continues for 900 years (from the conquest of England by the Normans to the end of World War II) of the anglicization of the French language and the gallicization of English, became the level of the phraseological system. The purpose of the article is to state the mutual enrichment of national phraseological funds and the replenishment of the system of images. The interaction of English and French in the field of phraseological borrowing is due to the fact that phraseological borrowing is the result of the interaction of these two languages and the history of two peoples, including the formation and development of their literature. The duration of language contact has provided the ability to perceive emotional images in which reality is displayed, even in the presence of different mentalities and different national traits

Quality profile of milk from high producing dairy cows vaccinated against mastitis

One of the raw milk quality criteria is the count of somatic cells, produced by the cow’s immune system to fight infectious diseases of the mammary gland. The paper presents the analysis of somatic cell count and total bacteria count of milk from cows, vaccinated against mastitis using Startvac vaccine. Tests were performed as a comparison between a dairy unit and a farm under different management conditions and using different milking techniques. Six months after the start of the vaccine application the somatic cell count at the dairy unit decreased by 60 thousand/ml, at the farm by 182 thousand/ml. The agent profile was represented by the following bacteria: Entеrococcus faecium, Staphylococcus aureus, Streptococcus spp., Pseudomonas aeruginosa. Staphylococcus saprophyticus, Staphylococcus epidermidis, Entеrococcus faecalis, Escherichia coli, Bacillus, Lactobacillus were also isolated from udder secretion. After a year of immunization somatic cell count both at the unit and on the farm decreased by 245 and 216 thousand/ml respectively; it is noteworthy that 43.75% of microflora isolated from mammary gland secretion was represented by Streptococcus spp. After two years of the vaccine use the somatic cell count was equal to 371 and 725 thousand/ml at the unit and on the farm respectively. Tests of mammary gland secretions revealed Streptococcus spp. in 27.27% of cases, Staphylococcus aureus and Entеrococcus faecium were isolated in 18.18% of tested samples. It was established that after three years of the vaccine use the major cause of mastitis in cows was Streptococcus spp. (55.00%). During four years of tests, a downward trend in somatic cell count of bulk milk from high producing dairy cows as well as in the number of agents responsible for inflammation in a mammary gland was detected. Somatic cell count of milk from vaccinated animals decreased by 286 and 432 thousand/ml at the unit and on the farm respectively. During the test period Staphylococcus aureus isolation rate declined by 19.41%