Utrasonographic monitoring of uterine motility in infertile women with adenomyosis = Ультрасонографический мониторинг маточной перистальтики у бесплодных женщин с аденомиозом

Gladchuk I. Z., Rogachev A. P., Garbuzenko N. D., Stamova N. A. Utrasonographic monitoring of uterine motility in infertile women with adenomyosis = Ультрасонографический мониторинг маточной перистальтики у бесплодных женщин с аденомиозом. Journal of Education, Health and Sport. 2015;5(12):691-700. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.44823http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%2812%29%3A691-700https://pbn.nauka.gov.pl/works/695960Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011–2014http://journal.rsw.edu.pl/index.php/JHS/issue/archive     The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport (null) 2391-8306 7© The Author (s) 2015;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 20.12.2015. Revised 25.12.2015. Accepted: 29.12.2015.     UTRASONOGRAPHIC MONITORING OF UTERINE MOTILITY IN INFERTILE WOMEN WITH ADENOMYOSISУльтрасонографический мониторинг маточной перистальтики у бесплодных женщин с аденомиозом I. Z. Gladchuk, A. P. Rogachev, N. D. Garbuzenko, N. A. StamovaИ. З. Гладчук, А. П. Рогачевский, Н. Д. Гарбузенко, Н. А. Стамова Odessa National Medical University, UkraineОдесский национальный медицинский университет, Одесса, Украина AbstractTaking into account that the uterine pump disruption is one of the leading pathogenic links of infertility in external endometriosis, a significant role of this factor can be detected and in adenomyosis. Diagnosis of uterine peristalsis held with ultrasonography (US), and the study of intrauterine transport was made by hysterosalpingoscintigraphy. Disperistaltic waves at were revealed predominantly in periovulatory phase with the average rate of 4.8 ± 0.23 waves / min. In the control group only single disperistaltic waves throughout the cycle, and their frequency did not exceed 0.4 ± 0.11 waves / min. In all the women with the lack of intrauterine transport  either disperistaltic or complete absence of subendometrial layers of myometrium contractions wave were found.  The significant increase of hysterosalpingoscintigraphy negative results and  contralateral transport depending on the patients’ age was revealed (p <0.01 and p <0.05, respectively). Therefore, patients with adenomyosis and impaired uterine peristaltic older than 30 years old should be recommended one of the techniques of extracorporal fertilization with embryo transfer technology, except for intrauterine insemination.Key words: adenomyosis, infertility, uterine pump, uterine peristalsis. Реферат Учитывая, что нарушение работы маточной помпы - одно из ведущих звеньев патогенеза бесплодия при наружном эндометриозе, может быть обнаружена значимая роль этого фактора и при аденомиозе. Диагностика маточной перистальтики проводится при ультрасонографии (УЗИ), а исследование внутриматочного транспорта – методом  гистеросальпингосцинтиграфии (ГССГ). Дисперистальтические волны при аденомиозе обнаружены преимущественно в периовуляторной фазе со средней частотой 4,8±0,23 волн/мин. В контрольной группе наблюдались только единичные дисперистальтические волны на протяжении всего цикла и их частота не превышала 0,4±0,11 волн/мин. У всех женщин с отсутствием внутриматочного транспорта обнаружены дисперистальтика или полное отсутствие волн сокращения субэндометриальных слоев миометрия. В ходе исследований обнаружено, достоверное увеличение числа случаев отрицательных результатов ГССГ и контралатерального транспорта с возрастом пациенток (р<0,01 и p<0,05, соответственно). Поэтому пациенткам с аденомиозом и нарушением маточной перистальтики старше 30 лет следует рекомендовать одну из методик экстракорпорального оплодотворения с технологией переноса эмбриона, исключая внутриматочную инсеминацию.Ключевые слова: аденомиоз, бесплодие, маточная помпа, маточная перистальтика

Ультрасонографический мониторинг особенностей маточной перистальтики у женщин с эндометриоз-ассоциируемой формой бесплодия

Uterine peristalsis studies have been conducted in 60 women with endometriosis-associated infertility. Violations of uterine peristalsis with adenomyosis in the form of dispersal waves were found, which practically are not determined in practically healthy women. The frequency of disperitostatic waves in patients with adenomyosis is significantly higher than that of practically healthy women (p<0,001). A significant decrease in the number of waves of type A and B in patients with adenomyosis (p<0,02) is determined.Проведено исследование маточной перистальтики у 60 женщин с эндометриоз-ассоциируемой формой бесплодия. Выявлено нарушение маточной перистальтики при аденомиозе в виде волн дисперистальтики, которые почти не определяются у практически здоровых женщин. Частота дисперистальтических волн у пациенток с аденомиозом достоверно выше, чем у практически здоровых женщин (р<0,001). Определяется достоверное снижение числа волн типа A и В у пациенток с аденомиозом (р<0,02)

Correlations of Gene Expression with Blood Lead Levels in Children with Autism Compared to Typically Developing Controls

The objective of this study was to examine the correlation between gene expression and lead (Pb) levels in blood in children with autism (AU, n = 37) compared to typically developing controls (TD, n = 15). We postulated that, though lead levels did not differ between the groups, AU children might metabolize lead differently compared to TD children. RNA was isolated from blood and processed on Affymetrix microarrays. Separate analyses of covariance (ANCOVA) corrected for age and gender were performed for TD, AU, and all subjects (AU + TD). To reduce false positives, only genes that overlapped these three ANCOVAs were considered. Thus, 48 probe sets correlated with lead levels in both AU and TD subjects and were significantly different between the groups (p(Diagnosis × log2 Pb) < 0.05). These genes were related mainly to immune and inflammatory processes, including MHC Class II family members and CD74. A large number (n = 791) of probe sets correlated (P ≤ 0.05) with lead levels in TD but not in AU subjects; and many probe sets (n = 162) correlated (P ≤ 0.05) with lead levels in AU but not in TD subjects. Only 30 probe sets correlated (P ≤ 0.05) with lead levels in a similar manner in the AU and TD groups. These data show that AU and TD children display different associations between transcript levels and low levels of lead. We postulate that this may relate to the underlying genetic differences between the two groups, though other explanations cannot be excluded

Correlations Between Gene Expression and Mercury Levels in Blood of Boys With and Without Autism

Gene expression in blood was correlated with mercury levels in blood of 2- to 5-year-old boys with autism (AU) compared to age-matched typically developing (TD) control boys. This was done to address the possibility that the two groups might metabolize toxicants, such as mercury, differently. RNA was isolated from blood and gene expression assessed on whole genome Affymetrix Human U133 expression microarrays. Mercury levels were measured using an inductively coupled plasma mass spectrometer. Analysis of covariance (ANCOVA) was performed and partial correlations between gene expression and mercury levels were calculated, after correcting for age and batch effects. To reduce false positives, only genes shared by the ANCOVA models were analyzed. Of the 26 genes that correlated with mercury levels in both AU and TD boys, 11 were significantly different between the groups (P(Diagnosis*Mercury) ≤ 0.05). The expression of a large number of genes (n = 316) correlated with mercury levels in TD but not in AU boys (P ≤ 0.05), the most represented biological functions being cell death and cell morphology. Expression of 189 genes correlated with mercury levels in AU but not in TD boys (P ≤ 0.05), the most represented biological functions being cell morphology, amino acid metabolism, and antigen presentation. These data and those in our companion study on correlation of gene expression and lead levels show that AU and TD children display different correlations between transcript levels and low levels of mercury and lead. These findings might suggest different genetic transcriptional programs associated with mercury in AU compared to TD children

Atypical miRNA expression in temporal cortex associated with dysregulation of immune, cell cycle, and other pathways in autism spectrum disorders

BACKGROUND: Autism spectrum disorders (ASDs) likely involve dysregulation of multiple genes related to brain function and development. Abnormalities in individual regulatory small non-coding RNA (sncRNA), including microRNA (miRNA), could have profound effects upon multiple functional pathways. We assessed whether a brain region associated with core social impairments in ASD, the superior temporal sulcus (STS), would evidence greater transcriptional dysregulation of sncRNA than adjacent, yet functionally distinct, primary auditory cortex (PAC). METHODS: We measured sncRNA expression levels in 34 samples of postmortem brain from STS and PAC to find differentially expressed sncRNA in ASD compared with control cases. For differentially expressed miRNA, we further analyzed their predicted mRNA targets and carried out functional over-representation analysis of KEGG pathways to examine their functional significance and to compare our findings to reported alterations in ASD gene expression. RESULTS: Two mature miRNAs (miR-4753-5p and miR-1) were differentially expressed in ASD relative to control in STS and four (miR-664-3p, miR-4709-3p, miR-4742-3p, and miR-297) in PAC. In both regions, miRNA were functionally related to various nervous system, cell cycle, and canonical signaling pathways, including PI3K-Akt signaling, previously implicated in ASD. Immune pathways were only disrupted in STS. snoRNA and pre-miRNA were also differentially expressed in ASD brain. CONCLUSIONS: Alterations in sncRNA may underlie dysregulation of molecular pathways implicated in autism. sncRNA transcriptional abnormalities in ASD were apparent in STS and in PAC, a brain region not directly associated with core behavioral impairments. Disruption of miRNA in immune pathways, frequently implicated in ASD, was unique to STS. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13229-015-0029-9) contains supplementary material, which is available to authorized users

NK cells and cancer: you can teach innate cells new tricks

Natural killer (NK) cells are the prototype innate lymphoid cells endowed with potent cytolytic function that provide host defence against microbial infection and tumours. Here, we review evidence for the role of NK cells in immune surveillance against cancer and highlight new therapeutic approaches for targeting NK cells in the treatment of cancer