Inverse problems in demography and biodemography

Inverse problems play important role in science and engineering. Estimation of boundary conditions on the temperature distribution inside a metallurgical furnace, reconstruction of tissue density inside body on plane projections obtained with x-rays are examples. The similar problems exist in demography in the form of projection and estimation of population age distributions and age-specific mortality rates. The problem of residual demography is estimation of demographic process in wild nature on its manifestation in marked subjects with unobserved age, which again is inverse problem. The article presents examples and the ways of solution the inverse problems in demography and biodemography, discusses the ways of improving results by combination of demographic and genetic data.

Senescence can play an essential role in modelling and estimation of vector based epidemiological indicators: demographical approach

In the paper basic epidemiological indicators, produced by an aging population of vectors, are calculated. In the study we follow two lines: calculations for demographically structured population and individual life-history approach. We discuss the advantages and limitations of these approaches and compare the results of our calculations with epidemiological indicators obtained for non-aging population of vectors.Gibraltar, age effect, disease control, gerontology

Conditions for nonexistence of static or stationary, Einstein-Maxwell, non-inheriting black-holes

We consider asymptotically-flat, static and stationary solutions of the Einstein equations representing Einstein-Maxwell space-times in which the Maxwell field is not constant along the Killing vector defining stationarity, so that the symmetry of the space-time is not inherited by the electromagnetic field. We find that static degenerate black hole solutions are not possible and, subject to stronger assumptions, nor are static, non-degenerate or stationary black holes. We describe the possibilities if the stronger assumptions are relaxed.Comment: 19 pages, to appear in GER

Non-woven polypropylene fabric modified with carbon nanotubes and decorated with nanoakaganeite for arsenite removal

Due to its harmful impact on human health, the presence of heavy metals, metalloids and other toxic pollutants in drinking or irrigation water is a major concern. Recent studies have proved that nanosized adsorbents are significantly more effective than their microsized counterparts. Particular attention has been given to nanocomposites with nanoadsorbents embedded in matrixes that could provide stability to the material and contribute to eliminating problems that may appear when using conventional granular systems. This study presents the preparation of a novel hybrid filter from a commercially available polypropylene (PP) non-woven fabric matrix modified with multiwall carbon nanotubes (MWCNT) and iron oxy(hydroxide) nanoparticles, and its use in the removal of As(III). A Box–Behnken statistical experimental design has been chosen to explore relevant variables affecting the filter performance: (1) As(III) concentration, (2) pH and (3) sorbent dose. From an As(III) concentration of 10 mg L−1, at pH 6.5 and with a sorbent dose of 5 g L−1, the PP filter modified with MWCNT removes 10% of the initial metalloid concentration, reaching a capacity of 0.27 mg g−1. After modification with iron oxy(hydroxide), the performance of the material is largely enhanced. The filter, under the same conditions, removes 90% of the initial As(III) concentration, reaching a capacity almost tenfold higher (2.54 mg g−1). This work demonstrates that the developed hybrid filter is effective toward the removal of As(III) in a wide range of pHs. A cubic regression model to compute the removal of the filter as a function of pH and sorbent dose is provided.acceptedVersio

Agonist-Specific Desensitization of PGE2-Stimulated cAMP Signaling due to upregulated Phosphodiesterase Expression in Human Lung Fibroblasts

Pulmonary fibrosis is characterized by fibroblasts persisting in an activated form, producing excessive fibrous material that destroys alveolar structure. The second messenger molecule cyclic 3′,5′-adenosine monophosphate (cAMP) has antifibrotic properties, and prostaglandin E2 (PGE2) can stimulate cAMP production through prostaglandin E (EP)2 and EP4 receptors. Although EP receptors are attractive therapeutic targets, the effects of long-term exposure to PGE2 have not been characterized. To determine the effects of long-term exposure of lung fibroblasts to PGE2, human fetal lung (HFL)-1 cells were treated for 24 h with 100 nM PGE2 or other cAMP-elevating agents. cAMP levels stimulated by acute exposure to PGE2 were measured using a fluorescent biosensor. Pretreatment for 24 h with PGE2 shifted the concentration-response curve to PGE2 rightward by approximately 22-fold but did not affect responses to the beta-adrenoceptor agonist isoproterenol. Neither isoproterenol nor forskolin pretreatment altered PGE2 responses, implying that other cAMP-elevating agents do not induce desensitization. Use of EP2- and EP4-selective agonists and antagonists suggested that PGE2-stimulated cAMP responses in HFL-1 cells are mediated by EP2 receptors. EP2 receptors are resistant to classical mechanisms of agonist-specific receptor desensitization, so we hypothesized that increased PDE activity mediates the loss of signaling after PGE2 pretreatment. PGE2 treatment upregulated messenger RNA for PDE3A, PDE3B, PDE4B, and PDE4D and increased overall PDE activity. The PDE4 inhibitor rolipram partially reversed PGE2- mediated desensitization and PDE4 activity was increased, but rolipram did not alter responses to isoproterenol. The PDE3 inhibitor cilostazol had minimal effect. These results show that long-term exposure to PGE2 causes agonist-specific desensitization of EP2 receptor-stimulated cAMP signaling through the increased expression of PDE isozymes, most likely of the PDE4 family

Survey of Nutrition Management Practices in Centers for Pediatric Intestinal Rehabilitation

Background: Nutrition management of pediatric intestinal failure (IF) requires interdisciplinary coordination of parenteral nutrition (PN) and enteral nutrition (EN) support. Nutrition strategies used by specialists in pediatric intestinal rehabilitation to promote gut adaptation and manage complications have not been previously summarized. Methods: A practice survey was distributed to members of the dietitian subgroup of the American Society for Parenteral and Enteral Nutrition Pediatric Intestinal Failure Section. The survey included 24 open‐ended questions related to PN and enteral feeding strategies, nutrition management of PN‐associated liver disease, and laboratory monitoring. Results: Dietitians from 14 centers completed the survey. Management components for patients at risk for cholestasis were consistent and included fat minimization, trace element modification, avoiding PN overfeeding, and providing EN. Parenteral amino acid solutions designed for infants/young children are used in patients <1 or 2 years of age. Trace minerals are dosed individually in 10 of 14 centers. Eleven centers prescribe a continuous infusion of breast milk or elemental formula 1–2 weeks after resection while 3 centers determine the formula type by the extent of resection. Most (86%) centers do not have a protocol for initiating oral/motor therapy. Laboratory panel composition varied widely by center. The selection and frequency of use depended on clinical variables, including cholestatic status, exclusive vs partial PN dependence, postrepletion verification vs routine monitoring, intestinal anatomy, and acuity of care. Conclusion: EN and PN management strategies are relatively consistent among U.S. centers. Collaborative initiatives are necessary to define better practices and establish laboratory monitoring guidelines.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/145220/1/ncp10040_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/145220/2/ncp10040.pd

The Impact II, a Very High-Resolution Quadrupole Time-of-Flight Instrument (QTOF) for Deep Shotgun Proteomics

Hybrid quadrupole time-of-flight (QTOF) mass spectrometry is one of the two major principles used in proteomics. Although based on simple fundamentals, it has over the last decades greatly evolved in terms of achievable resolution, mass accuracy, and dynamic range. The Bruker impact platform of QTOF instruments takes advantage of these developments and here we develop and evaluate the impact II for shotgun proteomics applications. Adaption of our heated liquid chromatography system achieved very narrow peptide elution peaks. The impact II is equipped with a new collision cell with both axial and radial ion ejection, more than doubling ion extraction at high tandem MS frequencies. The new reflectron and detector improve resolving power compared with the previous model up to 80%, i.e. to 40,000 at m/z 1222. We analyzed the ion current from the inlet capillary and found very high transmission (>80%) up to the collision cell. Simulation and measurement indicated 60% transfer into the flight tube. We adapted MaxQuant for QTOF data, improving absolute average mass deviations to better than 1.45 ppm. More than 4800 proteins can be identified in a single run of HeLa digest in a 90 min gradient. The workflow achieved high technical reproducibility (R2 > 0.99) and accurate fold change determination in spike-in experiments in complex mixtures. Using label-free quantification we rapidly quantified haploid against diploid yeast and characterized overall proteome differences in mouse cell lines originating from different tissues. Finally, after high pH reversed-phase fractionation we identified 9515 proteins in a triplicate measurement of HeLa peptide mixture and 11,257 proteins in single measurements of cerebellum-the highest proteome coverage reported with a QTOF instrument so far

Ocena wpływu zmiennego pola magnetycznego na wybrane parametry pracy serca - doniesienie wstępne

Wstęp: W pracy przedstawiono wyniki działania magnetoterapii na czynność serca pacjentów, u których prowadzono w warunkach klinicznych lecznicze zabiegi rehabilitacyjne po przebytych wcześniej urazach kręgosłupa lędźwiowego oraz stawów biodrowych. Materiał i metody: Stosowano przewidziane w takich przypadkach sinusoidalnie zmienne pola magnetyczne o następujących parametrach: indukcja 0,3 mT, częstotliwość 30 Hz (w rehabilitacji kręgosłupa lędźwiowego) oraz odpowiednio: 0,5 mT i 50 Hz (w rehabilitacji stawów biodrowych). Ocenę przeprowadzono na podstawie analizy nieliniowych parametrów krzywych obrazujących akcję serca: eksponent skalowania, które wyznaczono metodą DFA (detrended fluctuation analysis), diagramów Poincarego i entropii. Parametry te wyznaczono z pomiarów sygnałów fonokardiograficznych i elektrokardiograficznych, przeprowadzonych w trakcie działania pola magnetycznego oraz w 5-minutowych okresach bezpośrednio przed jego zastosowaniem i po nim. Wyniki i wnioski: W analizie otrzymanych wyników nie stwierdzono istotnych różnic pomiędzy rezultatami pomiarów na podstawie fonokardiogramu, jakie miały miejsce w trakcie działania pola magnetycznego oraz przed jego zastosowaniem i po tym okresie. Podobne wnioski dotyczą również analizy EKG zarejestrowanego przed magnetoterapią i po jej zastosowaniu. (Folia Cardiol. 2003; 10: 695&#8211;700

PGE 2

In the current study, we investigated the effect of a long-acting β-agonist (salmeterol) and a phosphodiesterase 4 (PDE4) inhibitor (cilomilast) on human lung fibroblast-mediated collagen gel contraction. Higher concentrations of salmeterol (10(−7) and 10(−6) M) inhibited fibroblast-mediated collagen gel contraction. No effect was observed with cilomilast alone (up to 10(−5) M). In the presence of 10(−8) M salmeterol, however, cilomilast could significantly inhibit fibroblast-mediated collagen gel contraction in a concentration-dependent manner (10(−7) ~10(−5) M). Blockade of endogenous PGE(2) by indomethacin further potentiated the inhibitory effect of salmeterol on fibroblast-mediated collagen gel contraction, but it did not affect cilomilast's effect. Pretreatment with PGE(2) abolished the inhibitory effect of salmeterol, but it potentiated the inhibitory effect of cilomilast on fibroblast-mediated collagen gel contraction. Finally, indomethacin slightly inhibited PDE4C expression, while PGE(2) stimulated the expression of PDE4A and -4C in human lung fibroblasts. These findings suggest that long-acting β-agonist and PDE4 inhibitor have a synergistic effect in regulating fibroblast tissue repair functions and that PGE(2) can modulate the effect of β-agonist and PDE4 inhibitor at least in part through the mechanism of regulating PDE4 expression