Structure determination of Split-soret Cytochrome from a Desulfovibrio species isolated from a human abdominal abcess

The determined structure of the split-soret cytochrome (SSC) isolated from Desulfovibrio desulfuricans ATCC 27774 (D.d.) revealed a new Heme arrangement, which suggests that this protein constitutes a new cytochrome class.. SSC is a 52.6kDa homodimer containing four hemes at one end of the molecule. In each monomer the two hemes have their edges overlapped within van der Waals contacts. The polypeptide chain of each monomer supplies the sixth ligand to the heme-iron of the other monomer. A similar protein was recently purified from a homologous Desulfovibrio clinical strain isolated from an abdominal wall abscess in human patient2. Crystals of this SSC were grown using vapour diffusion method in the presence of agarose gel. Diffraction data were collected using X-ray synchrotron radiation at the ESRF, beamline, ID 14-1. The structure will be solved by molecular replacement using the structure of the D.d. as a starting model

The mean energy, strength and width of triple giant dipole resonances

We investigate the mean energy, strength and width of the triple giant dipole resonance using sum rules.Comment: 12 page

Recovery of active pathogenesis-related enzymes from the apoplast of Musa acuminata infected by Mycosphaerella fijiensis

The fungus Mycosphaerella fijiensis causes black Sigatoka (BS) disease, a major pathogen in the banana industry worldwide. Numerous molecular and biochemical studies have been done for the M. fijiensis, Musa acuminata interaction, but this is the first study describing the zymographic behavior of β-1,3-glucanase, chitinase and protease in the apoplast and symplast of healthy, BS-infected but asymptomatic and BS diseased banana leaves. In BS-infected tissues, β-1,3-glucanase enzymatic activity was associated with two polypeptides with retention index (Ri) values of 0.43 and 0.56. These were more notable in the apoplast than in the symplast. Chitinase activity in BS-infected tissue in both the apoplast and symplast was mainly associated with a single polypeptide (Ri = 0.89). Both β-1,3-glucanase and chitinase activities were apparently more intense in BS-infected leaves than in healthy leaves. Protease activity was associated with two polypeptides (Ri = 0.04 and 0.14). In both the apoplast and symplast, the Ri 0.04 polypeptide increased in intensity with disease progression, whereas Ri 0.14 polypeptide intensity decreased. Overall protease activity intensity was higher in the symplast. Maximum symplast contamination of the apoplast was 2% as estimated by glucose 6-phosphate dehydrogenase activity, a biochemical marker for symplast. Accumulation of pathogenesis-related enzymatic activities in the apoplast of M. acuminata leaf tissue was caused by hostcontrolled enzyme downloading in response to M. fijiensis infection. Clear differences were identified in the electrophoretic profiles of healthy and diseased banana plants. The results further support a putative role of these enzymes in the extracellular defense repertoire of banana and, more importantly, suggest that M. fijiensis possesses a mechanism for suppression and delay of defense response in M. acuminata.Key words: Black Sigatoka, glucose 6-phosphate dehydrogenase, pathogenesis-related (PR) proteins, polyacrylamide gel electrophoresis (PAGE), retention index (Ri), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)

Stellar parameters for stars of the CoRoT exoplanet field

Aims:To support the computation and evolutionary interpretation of periods associated with the rotational modulation, oscillations, and variability of stars located in the CoRoT fields, we are conducting a spectroscopic survey for stars located in the fields already observed by the satellite. These observations allow us to compute physical and chemical parameters for our stellar sample. Method: Using spectroscopic observations obtained with UVES/VLT and Hydra/Blanco, and based on standard analysis techniques, we computed physical and chemical parameters ($T_{\rm{eff}}$, $\log \,(g)$, $\rm{[Fe/H]}$, $v_{\rm{mic}}$, $v_{\rm{rad}}$, $v \sin \,(i)$, and $A(\rm{Li})$) for a large sample of CoRoT targets. Results: We provide physical and chemical parameters for a sample comprised of 138 CoRoT targets. Our analysis shows the stars in our sample are located in different evolutionary stages, ranging from the main sequence to the red giant branch, and range in spectral type from F to K. The physical and chemical properties for the stellar sample are in agreement with typical values reported for FGK stars. However, we report three stars presenting abnormal lithium behavior in the CoRoT fields. These parameters allow us to properly characterize the intrinsic properties of the stars in these fields. Our results reveal important differences in the distributions of metallicity, $T_{\rm eff}$, and evolutionary status for stars belonging to different CoRoT fields, in agreement with results obtained independently from ground-based photometric surveys. Conclusions: Our spectroscopic catalog, by providing much-needed spectroscopic information for a large sample of CoRoT targets, will be of key importance for the successful accomplishment of several different programs related to the CoRoT mission, thus it will help further boost the scientific return associated with this space mission.Comment: 43 pages, 17 figures, accepted for publication in A&

Accretion Disks Around Young Objects. II. Tests of Well-Mixed Models with Ism Dust

We construct detailed vertical structure models of irradiated accretion disks around T Tauri stars with interstellar medium dust uniformly mixed with gas. The dependence of the structure and emission properties on mass accretion rate, viscosity parameter, and disk radius is explored using these models. The theoretical spectral energy distributions (SEDs) and images for all inclinations are compared with observations of the entire population of Classical T Tauri stars (CTTS) and Class I objects in Taurus. In particular, we find that the median near-infrared fluxes can be explained within the errors with the most recent values for the median accretion rates for CTTS. We further show that the majority of the Class I sources in Taurus cannot be Class II sources viewed edge-on because they are too luminous and their colors would be consistent with disks seen only in a narrow range of inclinations. Our models appear to be too geometrically thick at large radii, as suggested by: (a) larger far-infrared disk emission than in the typical SEDs of T Tauri stars; (b) wider dark dust lanes in the model images than in the images of HH30 and HK Tau/c; and (c) larger predicted number of stars extincted by edge-on disks than consistent with current surveys. The large thickness of the model is a consequence of the assumption that dust and gas are well-mixed, suggesting that some degree of dust settling may be required to explain the observations.Comment: 41 pages, 13 figures, accepted in Ap