Development of Probes for Bile Acid Transporters and Synthetic Approaches to the Core of Massadine

My graduate work comprises two components: 1) The development of novel probes to study the activities of bile acid (BA) by exploiting a self-cleavable disulfide linker. 2) The synthetic studies toward the racemic and enantioselective versions of a carbocycle core of massadine wherein the application of a formal [3+2] cycloaddition of α,β-unsaturated esters and lithium(trimethylsilyl)diazomethane (LTMSD) is used as a key strategy. The first part involves the design and development of a bile acid (BA) transporter probe for investigating BA transporter activity in real time. BA probes (BA-SS-Luc) containing a luciferin moiety connected via a disulfide cleavable linker were found to be useful tool to measure bile acid uptake in real time in vitro. For the future study, this quantitative approach will be applied to identify compounds that modulate BA transporter activity in vivo. The second part deals with synthetic studies of a carbocycle core of massadine, which is a member of structurally complex dimeric pyrrole- imidazole alkaloids. Due to its unique structural diversity, complexity and significant biological activities, massadine, has been a highly sought- after target for total synthesis among many research groups. Our synthetic approach toward the synthesis of the core skeleton relies on the formal [3+2] cycloaddition between LTMSD and α,β-unsaturated esters followed by protonylitic N–N bond cleavage to construct α-amino-β-cyano groups with excellent stereochemical control. These functional groups would act as a synthetic handle to construct the remaining structural features of massadine

Visual Poetry in the Network of the International Communication. Terms, Categories, Typology

The doctoral thesis is concerned diachronically with the term "visual poetry". The focus is on its progress during the period after the Second World War in the context of the extensively advanced movement, as substantially defined in international expert discussion. The use of the term, which is supported by the arguments of many theoreticians and artists of the period, is studied in the thesis from the viewpoint of the inspirational sources and specific contacts between art and literature. The focus is on the specific positions and strategies of visual perception. Visual poetry is studied - considering the different contexts of the European avant-garde movements and through the analysis of contemporaneous theories which defined three main lines of thought - as an incoherent art stream manifesting itself in the eclectic use of artistic media. In conclusion, the thesis looks at the project of Czech author poetics typology which grew from purely Czech examples based on formal language of the visual poetry works. The thesis seeks to point out the original aspects of the internal discussion of visual poetry, and to place it in the international context

MOESM6 of Acute immobilization stress following contextual fear conditioning reduces fear memory: timing is essential

Additional file 6: Table S6. Tukey HSD for acetylation H3K14 at promoter 3 (Experiment 4)

MOESM8 of Acute immobilization stress following contextual fear conditioning reduces fear memory: timing is essential

Additional file 8: Table S8. Tukey HSD for corticosterone analysis (Experiment 5)

MOESM10 of Acute immobilization stress following contextual fear conditioning reduces fear memory: timing is essential

Additional file 10: Table S10. Tukey HSD for mRNA 2 (Experiment 6)