Developing parametric design fashion products using 3D printing technology

This study created wearable fashion products with parametric design characteristics, using 3D printing technology. The goal of the study was to understand what parametric design features can be simulated with 3D modeling and printing technology, as well as to demonstrate what techniques can be used to produce fashion products using 3D printing technology. This study created two different parametric motifs using an FDM-type 3D printer with TPU and ABS as the printing materials. With those motifs, we produced three garments and two accessories. The limitations found during the process were modeling the exact measurement of the motifs that will merge with the apparel design seamlessly while maintaining the parametric features, as well as attaching the printed motifs to fabric without ruining the integrity of the textile. A significant implication of this study is that it recreates parametric designs on the human body and utilizes 3D printing technology for fashion products. This paper cast a light on a discussion about the technique can be applied on fashion design with full-sized body and encouraged designers to explore further with technological advancements in the future

The effect of the stacking fault on the diffusion of chemisorbed hydrogen atoms inside few-layered graphene

We examined the diffusion of hydrogen atoms in mono-, bi- and tetralayer graphene with AB stacking and two bilayer graphene with stacking faults using density functional theory. The bi- and tetralayer graphene provide diffusion pathways with lower energy barriers inside the interlayer space. Inside the bi- and tetralayer graphene with AB stacking, the in-plane diffusion is more favorable than the inter-plane jumping. However, the stacking faults made by sliding layer planes lowers the energy barrier of the inter-plane jumping and the effective frequency of the inter-plane jump is larger than that of the in-plane diffusion inside the graphene layers with the stacking faults. This suggests that hydrogen atoms can diffuse over a long distance inside few-layered graphene with stacking faults jumping consecutively between adjacent layers.close0

Methionine deprivation suppresses triple-negative breast cancer metastasis in vitro and in vivo

Nutrient deprivation strategies have been proposed as an adjuvant therapy for cancer cells due to their increased metabolic demand. We examined the specific inhibitory effects of amino acid deprivation on the metastatic phenotypes of the human triple-negative breast cancer (TNBC) cell lines MDA-MB-231 and Hs 578T, as well as the orthotopic 4T1 mouse TNBC tumor model. Among the 10 essential amino acids tested, methionine deprivation elicited the strongest inhibitory effects on the migration and invasion of these cancer cells. Methionine deprivation reduced the phosphorylation of focal adhesion kinase, as well as the activity and mRNA expression of matrix metalloproteinases MMP-2 and MMP-9, two major markers of metastasis, while increasing the mRNA expression of tissue inhibitor of metalloproteinase 1 in MDA-MB-231 cells. Furthermore, methionine restriction downregulated the metastasis-related factor urokinase plasminogen activatior and upregulated plasminogen activator inhibitor 1 mRNA expression. Animals on the methionine-deprived diet showed lower lung metastasis rates compared to mice on the control diet. Taken together, these results suggest that methionine restriction could provide a potential nutritional strategy for more effective cancer therapy

Optimized Methods for the Isolation of Arabidopsis Female Central Cells and Their Nuclei

The Arabidopsis female gametophyte contains seven cells with eight haploid nuclei buried within layers of sporophytic tissue. Following double fertilization, the egg and central cells of the gametophyte develop into the embryo and endosperm of the seed, respectively. The epigenetic status of the central cell has long presented an enigma due both to its inaccessibility, and the fascinating epigenome of the endosperm, thought to have been inherited from the central cell following activity of the DEMETER demethylase enzyme, prior to fertilization. Here, we present for the first time, a method to isolate pure populations of Arabidopsis central cell nuclei. Utilizing a protocol designed to isolate leaf mesophyll protoplasts, we systematically optimized each step in order to efficiently separate central cells from the female gametophyte. We use initial manual pistil dissection followed by the derivation of central cell protoplasts, during which process the central cell emerges from the micropylar pole of the embryo sac. Then, we use a modified version of the Isolation of Nuclei TAgged in specific Cell Types (INTACT) protocol to purify central cell nuclei, resulting in a purity of 75-90% and a yield sufficient to undertake downstream molecular analyses. We find that the process is highly dependent on the health of the original plant tissue used, and the efficiency of protoplasting solution infiltration into the gametophyte. By isolating pure central cell populations, we have enabled elucidation of the physiology of this rare cell type, which in the future will provide novel insights into Arabidopsis reproduction.OAIID:RECH_ACHV_DSTSH_NO:T201620049RECH_ACHV_FG:RR00200001ADJUST_YN:EMP_ID:A076495CITE_RATE:2.67FILENAME:molce-39-10-768.pdfDEPT_NM:생명과학부EMAIL:[email protected]_YN:YFILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/4cccba36-07e6-4a85-9eda-27f8dd164627/linkCONFIRM:

Identification of antigen-presenting dendritic cells in mouse aorta and cardiac valves

Presumptive dendritic cells (DCs) bearing the CD11c integrin and other markers have previously been identified in normal mouse and human aorta. We used CD11c promoter–enhanced yellow fluorescent protein (EYFP) transgenic mice to visualize aortic DCs and study their antigen-presenting capacity. Stellate EYFP+ cells were readily identified in the aorta and could be double labeled with antibodies to CD11c and antigen-presenting major histocompatability complex (MHC) II products. The DCs proved to be particularly abundant in the cardiac valves and aortic sinus. In all aortic locations, the CD11c+ cells localized to the subintimal space with occasional processes probing the vascular lumen. Aortic DCs expressed little CD40 but expressed low levels of CD1d, CD80, and CD86. In studies of antigen presentation, DCs selected on the basis of EYFP expression or binding of anti-CD11c antibody were as effective as DCs similarly selected from the spleen. In particular, the aortic DCs could cross-present two different protein antigens on MHC class I to CD8+ TCR transgenic T cells. In addition, after intravenous injection, aortic DCs could capture anti-CD11c antibody and cross-present ovalbumin to T cells. These results indicate that bona fide DCs are a constituent of the normal aorta and cardiac valves

Comparative Mystical Theology of Love and Emptiness: Nondual Union in Buddhists and Beguines

The mode of ‘comparison’ is essential, especially when it comes to the contemplation of Marguerite Porete and Hadewijch, the French and Dutch Beguine mystics from 13th century; Their notoriously enigmatic mysticism, by which radical and unique meditations on indistinct union with Divine are expressed in erotic and ecstatic manner, may discover a counterpart in nondualist tantric traditions of Buddhism and the broader Indian discourse of self and soul. In short, I will argue for the significance of desire and emotion in the study of comparative mysticism. Chapter 2 “Nondual Anthropology of Nothingness and No-Self,” therefore, attempts an interpretation of French Beguine Porete, and her core notion of nothingness, first and foremost as Buddhist. In order to do so, the problem and method of comparative discourses will be examined and applied. I cope with this challenge by primarily showing how atypical, radical, and nontraditional Porete's theological anthropology of nothingness is; and how her mysticism actually becomes even clearer and more resonant when first compared to Buddhist philosophers such as Personalists (pudgalavadin), and more profoundly, the Tibetan other-emptiness followers (zhentongpa) whose teaching also contain an unorthodox interpretation of no-self and emptiness, both transcendental and unconventionally ‘theological’- introducing Tibetan zhentong philosopher Dölpopa, German mystic Eckhart as the counterparts. They serve the purpose of understanding Porete's nothingness, in nondual relation with Buddhist no-self, that is not total nonexistence of self. Having grounded philosophical commonalities between the Beguines and the Buddhists, Chapter 3 will historically survey between Porete's deification and divinization side-to-side by Buddhist Goddesses tradition, focusing on common features between the Beguine mystic and Buddhist tradition. The chapter reviews the past and current scholarship that has taken keen interest in female deities, divine-human union and the problem of gender, both from Christian and Buddhist fields. Chapter 4 is a prequel to the last, and the serious consideration of how love and desire play a role in mystical union. Here, the psychology in Dutch Beguine Hadewijch, focusing on her noted mode of despair, and love-madness as a genuine human response to the fluctuating experience of nondual divine-human union. I will engage with the relevant notion of ecstatic emotion in South Asian context, such as ‘Mahā-bhāva’― a term for ‘divine ecstasy’ or ‘religious madness,’ in the context of highest spiritual love toward the deity. Finally, in “Joy That is Bliss and Beatitude,” the dissertation culminates in addressing the enigmatic Porete’s joy, her union with Divine Love, modes of illumination via seeing and knowing. Porete’s joy, carrying the connotation of bliss and beatitude, finds profound affinity in Tibetan tantric Buddhist practices, with an influence of Kaśmīr Śaivism, a significant interlocutor of the French Beguine. I will make a tentative conclusion that it is the nondualist tantric union in Buddhism and Śaivism, that truly resonates with Porete best. As evidence, the fundamental concepts in South Asian rapture, such as the notion of aesthetic emotion (rasa) will be introduced, along with sense imageries and phenomena that intensify the ecstatic and erotic union

Immunogenicity of lipid nanoparticles and its impact on the efficacy of mRNA vaccines and therapeutics

Abstract Several studies have utilized a lipid nanoparticle delivery system to enhance the effectiveness of mRNA therapeutics and vaccines. However, these nanoparticles are recognized as foreign materials by the body and stimulate innate immunity, which in turn impacts adaptive immunity. Therefore, it is crucial to understand the specific type of innate immune response triggered by lipid nanoparticles. This article provides an overview of the immunological response in the body, explores how lipid nanoparticles activate the innate immune system, and examines the adverse effects and immunogenicity-related development pathways associated with these nanoparticles. Finally, we highlight and explore strategies for regulating the immunogenicity of lipid nanoparticles

Future changes in Antarctic coastal polynyas and bottom water formation simulated by a high-resolution coupled model

Abstract Antarctic coastal polynyas produce Dense Shelf Water, a precursor to Antarctic Bottom Waters that supply the global abyssal circulation. Future projections of Dense Shelf Water formation are hindered by unresolved small-scale atmosphere-sea ice-ocean interactions in polynyas. Here, we investigate the future evolution of Antarctic coastal polynyas using a high-resolution ocean-ice-atmosphere model. We find that wintertime sea ice production rates remain active even under elevated atmospheric CO2 concentrations. Antarctic winter sea ice production rates are sensitive to atmospheric CO2 concentrations: doubling CO2 (734 ppm) decreases sea ice production by only 6–8%, versus 10–30% under CO2 quadrupling (1468 ppm). While considerable uncertainty remains in future ice-shelf basal melting, which is not accounted for in this study, doubling or quadrupling CO2 substantially freshens Dense Shelf Water due to increased precipitation. Consequently, doubling CO2 weakens Dense Shelf Water formation by ~ 75%, while CO2 quadrupling shuts down Dense Shelf Water formation