3 research outputs found
Enhanced Osteogenic Commitment of Human Mesenchymal Stem Cells on Polyethylene Glycol-Based Cryogel with Graphene Oxide Substrate
Graphene
oxide (GO) is considered a comparatively recent biomaterial
with enormous potential because of its nontoxicity, high dispersity,
and enhanced interaction with biomolecules. These characteristics
of GO can promote the interactions between the substrates and cell
surfaces. In this study, we incorporated GO in a cryogel-based scaffold
system to observe their influence on the osteogenic responses of human
tonsil-derived mesenchymal stem cells (hTMSCs). Compared to polyethylene
glycol (PEG)-based cryogel scaffold, GO-embedded PEG-based (PEGDA-GO)
cryogels not only showed improved cell attachment and focal adhesion
kinase (FAK) signaling activation but also enhanced cell viability.
Taken together, we demonstrated that PEGDA-GO cryogels can stimulate
osteogenic differentiation under an osteoinductive condition and enhance
osteogenic phenotypes compared to the control group. In summary, we
demonstrate that GO embedded in cryogels system is an effective biofunctionalizing
scaffold to control osteogenic commitment of stem cells
General and Facile Coating of Single Cells via Mild Reduction
Cell surface modification has been
extensively studied to enhance
the efficacy of cell therapy. Still, general accessibility and versatility
are remaining challenges to meet the increasing demand for cell-based
therapy. Herein, we present a facile and universal cell surface modification
method that involves mild reduction of disulfide bonds in cell membrane
protein to thiol groups. The reduced cells are successfully coated
with biomolecules, polymers, and nanoparticles for an assortment of
applications, including rapid cell assembly, in vivo cell monitoring,
and localized cell-based drug delivery. No adverse effect on cellular
morphology, viability, proliferation, and metabolism is observed.
Furthermore, simultaneous coating with polyethylene glycol and dexamethasone-loaded
nanoparticles facilitates enhanced cellular activities in mice, overcoming
immune rejection
Chondroitin Sulfate-Based Biomineralizing Surface Hydrogels for Bone Tissue Engineering
Chondroitin
sulfate (CS) is the major component of glycosaminoglycan
in connective tissue. In this study, we fabricated methacrylated PEGDA/CS-based
hydrogels with varying CS concentration (0, 1, 5, and 10%) and investigated
them as biomineralizing three-dimensional scaffolds for charged ion
binding and depositions. Due to its negative charge from the sulfate
group, CS exhibited an osteogenically favorable microenvironment by
binding charged ions such as calcium and phosphate. Particularly,
ion binding and distribution within negatively charged hydrogel was
dependent on CS concentration. Furthermore, CS dependent biomineralizing
microenvironment induced osteogenic differentiation of human tonsil-derived
mesenchymal stem cells in vitro. Finally, when we transplanted PEGDA/CS-based
hydrogel into a critical sized cranial defect model for 8 weeks, 10%
CS hydrogel induced effective bone formation with highest bone mineral
density. This PEGDA/CS-based biomineralizing hydrogel platform can
be utilized for in situ bone formation in addition to being an investigational
tool for in vivo bone mineralization and resorption mechanisms